Rh2AG216700

protein At4g37920, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013303
Physical Location & Seq
Reverse (-)
22082678 .. 22085856
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG216700.1

Sequence Viewer

Length: 1299 bp
ATGAACAATCTGTTGGGACTTGAGCTTTCCACTTCCACAACCCACCACTCCATCTTCTTCAACACTTCAACCTCCTTTGACTTCTTCCCAACATTCAATGAAATCCTTTCCCCAACTACCCTTATCCCTCCCATATCCTCTGCCCCTCTCACTTCCAACCACAAAAGAAACACAAGAGCAACTCAATGCTTCAAAACCCGTGCTGCCCACTCAAGCCCCACAATCAGTCCTCATGTTCAAGTGGAGGAGCAAGTAGAGGTTGAAGTTGCTGAAGGGTACACCATCACTCAATTTTGTGATAAGATAATAGATATTTTCTTGAATGAGAAGCCTAGGGTAAAGGAATGGAGGAGGTATCTGGTGTTTAGGGAGGAGTGGAAGAAATATAGAGATTGCTTTTATAATAGGTGTAGGAATCGGGCCGATGCGGAGAGTGATGTAACTATGAAGGAAAAATTGGTTTCTATGGGAAGAAAGGTGAAGAAGATTGATGATGAAATGGAAAGACATAGTGAACTTCTCAAGGAGATACAGGATAACCCAACCGACGTCAATGCAATAGTTGCACAGAAGCGTAAAGACTTCACAGAGGAATTCTTTCGACACCTCAACCTACTTTCCGAAGTTTATGATGGTTTGGAAGATCGTGATGCAATGGCTCGGCTTGGGGCTAGATGCTTGTCTGCTGTCAGTGCTTATGATAACACATTAGAATATTTGGACACATTAGATACAGCCCAGGCCAAATTCAATGATATCCTAAGTTCACATTCAATCGATGTAGCTTGTGAGAAAATTAAAAGCCTTGCCAAGGCAAAGGAACTGGATTCTTCATTGGTATTATTGATAAACAGTGCTTGGGCTTCAGCAAAGGAGTCCACAACGATGAAAAATGAGGTCAAAGAAATAATGTATCGTTTGTACAAAGCCACAAAAAGCAGTCTTCGGAGCATTGTACCGAAAGAAATAAAGCTGCTCAAACATTTGCTGAACATAACAGATCCTGAAGAGCGATTCTCAGCACTGGCAACAGCTTTCTGCCCTGGTGATGATAATGAAGCCAAGGACCCCAAAGCTGTGTACAGTACTCCCAAAGAGCTTCACAAGTGGATTAAGATCATGCTCGATGCATACCATCTAAACGCAGAAGAAACTGACCTTAGGGAAGCCAGGCAGATGACTCAGCCTGTGGTTATACAAAGGCTGTTCATCCTCAAGGAAACTATTGAAGAGGAATATTTGGAAAAGAGCACATCTCCGGAGTCTCAAACAGAGGAAACTAAATCAGAGGAGTTGTGA

Protein Analysis

432

Amino Acids

49.76

Weight (kDa)

5.63

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G37920
fragaria_vesca FvH4_1g18990 FvH4_1g18990 FvH4_1g18990 FvH4_1g18990 FvH4_1g18990
malus_domestica MD01G1044700.v1.1
prunus_persica Prupe.6G209500_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0110011
rosa_laevigata RLG00000017777
rosa_multiflora Rmu_sc0001870.1_g000006
rosa_roxburghii Rroxscaffold_2G00133780
rosa_rugosa Rorug02G0165100
rosa_samantha Rh2AG216700 Rh2BG226700 Rh2CG218900 Rh2DG222300
rosa_wichuraiana Rw2G016750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AatII GACGTC 1 cut(s) 552
AccIII TCCGGA 1 cut(s) 1258
AciI CCGC 1 cut(s) 428
AclWI GGATC 1 cut(s) 995
AcsI RAATTY 2 cut(s) 593, 746
AcuI CTGAAG 3 cut(s) 291, 849, 1026
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 5 cut(s) 278, 923, 957, 1082, 1087
AfiI CCNNNNNNNGG 1 cut(s) 811
AjnI CCWGG 3 cut(s) 738, 1042, 1169
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 440, 472
AluBI AGCT 6 cut(s) 25, 785, 973, 1034, 1076, 1099
AluI AGCT 6 cut(s) 25, 785, 973, 1034, 1076, 1099
Alw21I GWGCWC 1 cut(s) 1253
Alw26I GTCTC 1 cut(s) 1269
AlwI GGATC 1 cut(s) 995
AlwNI CAGNNNCTG 1 cut(s) 1004
Aor13HI TCCGGA 1 cut(s) 1258
AoxI GGCC 2 cut(s) 420, 741
ApeKI GCWGC 2 cut(s) 203, 973
ApoI RAATTY 2 cut(s) 593, 746
Asp700I GAANNNNTTC 1 cut(s) 597
AspA2I CCTAGG 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 420, 1066
AsuHPI GGTGA 2 cut(s) 490, 1058
AvaII GGWCC 1 cut(s) 1066
AvrII CCTAGG 1 cut(s) 332
AxyI CCTNAGG 1 cut(s) 1160
BbsI GAAGAC 1 cut(s) 935
Bbv12I GWGCWC 1 cut(s) 1253
BbvI GCAGC 2 cut(s) 190, 960
BccI CCATC 4 cut(s) 59, 290, 626, 1143
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BciT130I CCWGG 3 cut(s) 740, 1044, 1171
BcoDI GTCTC 1 cut(s) 1269
BfaI CTAG 2 cut(s) 333, 672
BisI GCNGC 2 cut(s) 204, 974
BlnI CCTAGG 1 cut(s) 332
BlsI GCNGC 2 cut(s) 205, 975
BmcAI AGTACT 1 cut(s) 1087
Bme1390I CCNGG 3 cut(s) 740, 1044, 1171
Bme18I GGWCC 1 cut(s) 1066
BmgT120I GGNCC 2 cut(s) 420, 1066
BmiI GGNNCC 1 cut(s) 1068
BmrFI CCNGG 3 cut(s) 740, 1044, 1171
BmsI GCATC 4 cut(s) 415, 640, 665, 1117
BpiI GAAGAC 1 cut(s) 935
BplI GAGNNNNNCTC 4 cut(s) 1001, 1033, 1240, 1272
BpuEI CTTGAG 4 cut(s) 41, 196, 506, 1199
Bsa29I ATCGAT 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 1115
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 5 cut(s) 332, 738, 810, 1042, 1062
BsaWI WCCGGW 1 cut(s) 1258
Bsc4I CCNNNNNNNGG 1 cut(s) 811
Bse1I ACTGG 2 cut(s) 828, 1029
Bse21I CCTNAGG 1 cut(s) 1160
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 1115
BseAI TCCGGA 1 cut(s) 1258
BseBI CCWGG 3 cut(s) 740, 1044, 1171
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 5 cut(s) 332, 738, 810, 1042, 1062
BseGI GGATG 1 cut(s) 1209
BseJI GATNNNNATC 1 cut(s) 1115
BseLI CCNNNNNNNGG 1 cut(s) 811
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 2 cut(s) 1032, 1196
BseNI ACTGG 2 cut(s) 828, 1029
BseRI GAGGAG 3 cut(s) 260, 364, 386
BseXI GCAGC 2 cut(s) 190, 960
BshFI GGCC 2 cut(s) 422, 743
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 1253
BsiSI CCGG 1 cut(s) 1259
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 811
BsmAI GTCTC 1 cut(s) 1269
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 2 cut(s) 422, 743
Bsp1286I GDGCHC 1 cut(s) 1253
Bsp13I TCCGGA 1 cut(s) 1258
Bsp1407I TGTACA 2 cut(s) 921, 1080
Bsp143I GATC 3 cut(s) 643, 1000, 1116
BspACI CCGC 1 cut(s) 428
BspANI GGCC 2 cut(s) 422, 743
BspCNI CTCAG 2 cut(s) 1031, 1195
BspDI ATCGAT 1 cut(s) 777
BspEI TCCGGA 1 cut(s) 1258
BspLI GGNNCC 1 cut(s) 1068
BspPI GGATC 1 cut(s) 995
BspQI GCTCTTC 1 cut(s) 1002
BsrDI GCAATG 1 cut(s) 660
BsrGI TGTACA 2 cut(s) 921, 1080
BsrI ACTGG 2 cut(s) 828, 1029
BssECI CCNNGG 5 cut(s) 332, 738, 810, 1042, 1062
BssMI GATC 3 cut(s) 643, 1000, 1116
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 3 cut(s) 332, 810, 1062
Bst2UI CCWGG 3 cut(s) 740, 1044, 1171
Bst4CI ACNGT 2 cut(s) 854, 1085
Bst6I CTCTTC 2 cut(s) 1002, 1224
BstACI GRCGYC 1 cut(s) 549
BstAPI GCANNNNNTGC 1 cut(s) 563
BstAUI TGTACA 2 cut(s) 921, 1080
BstDEI CTNAG 4 cut(s) 761, 1018, 1160, 1182
BstENI CCTNNNNNAGG 1 cut(s) 809
BstF5I GGATG 1 cut(s) 1209
BstKTI GATC 3 cut(s) 646, 1003, 1119
BstMAI GTCTC 1 cut(s) 1269
BstMBI GATC 3 cut(s) 643, 1000, 1116
BstMWI GCNNNNNNNGC 2 cut(s) 563, 692
BstNI CCWGG 3 cut(s) 740, 1044, 1171
BstSCI CCNGG 3 cut(s) 738, 1042, 1169
BstV1I GCAGC 2 cut(s) 190, 960
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 1 cut(s) 1000
BstYI RGATCY 1 cut(s) 1000
Bsu15I ATCGAT 1 cut(s) 777
Bsu36I CCTNAGG 1 cut(s) 1160
BsuRI GGCC 2 cut(s) 422, 743
BsuTUI ATCGAT 1 cut(s) 777
BtsCI GGATG 1 cut(s) 1209
BtsIMutI CAGTG 3 cut(s) 697, 859, 1022
CaiI CAGNNNCTG 1 cut(s) 1004
Cfr13I GGNCC 2 cut(s) 420, 1066
ClaI ATCGAT 1 cut(s) 777
Csp6I GTAC 5 cut(s) 277, 922, 956, 1081, 1086
CviAII CATG 2 cut(s) 233, 1120
CviQI GTAC 5 cut(s) 277, 922, 956, 1081, 1086
DdeI CTNAG 4 cut(s) 761, 1018, 1160, 1182
DpnI GATC 3 cut(s) 645, 1002, 1118
DpnII GATC 3 cut(s) 643, 1000, 1116
Eam1104I CTCTTC 2 cut(s) 1002, 1224
EarI CTCTTC 2 cut(s) 1002, 1224
Eco130I CCWWGG 3 cut(s) 332, 810, 1062
Eco32I GATATC 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 1066
Eco57I CTGAAG 3 cut(s) 291, 849, 1026
Eco81I CCTNAGG 1 cut(s) 1160
EcoNI CCTNNNNNAGG 1 cut(s) 809
EcoO109I RGGNCCY 1 cut(s) 1066
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 738, 1042, 1169
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 3 cut(s) 332, 810, 1062
EcoT22I ATGCAT 1 cut(s) 1132
ErhI CCWWGG 3 cut(s) 332, 810, 1062
FaeI CATG 2 cut(s) 236, 1123
FaqI GGGAC 1 cut(s) 30
FatI CATG 2 cut(s) 232, 1119
Fnu4HI GCNGC 2 cut(s) 204, 974
FokI GGATG 1 cut(s) 1196
Fsp4HI GCNGC 2 cut(s) 204, 974
FspBI CTAG 2 cut(s) 333, 672
GluI GCNGC 2 cut(s) 204, 974
HaeIII GGCC 2 cut(s) 422, 743
HapII CCGG 1 cut(s) 1259
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 2 cut(s) 236, 1123
HinfI GANTC 6 cut(s) 415, 827, 875, 1014, 1180, 1262
HpaII CCGG 1 cut(s) 1259
HphI GGTGA 2 cut(s) 490, 1058
Hpy166II GTNNAC 5 cut(s) 279, 515, 767, 879, 1081
Hpy188I TCNGA 3 cut(s) 622, 948, 1288
Hpy188III TCNNGA 4 cut(s) 319, 647, 1004, 1259
Hpy8I GTNNAC 5 cut(s) 279, 515, 767, 879, 1081
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 2 cut(s) 266, 442
HpyCH4III ACNGT 2 cut(s) 854, 1085
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 4 cut(s) 557, 566, 653, 1130
HpyF10VI GCNNNNNNNGC 2 cut(s) 563, 692
HpyF3I CTNAG 4 cut(s) 761, 1018, 1160, 1182
HpySE526I ACGT 1 cut(s) 549
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 2 cut(s) 236, 1123
Kpn2I TCCGGA 1 cut(s) 1258
Kzo9I GATC 3 cut(s) 643, 1000, 1116
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 2 cut(s) 247, 948
Lsp1109I GCAGC 2 cut(s) 190, 960
LweI GCATC 4 cut(s) 415, 640, 665, 1117
MaeI CTAG 2 cut(s) 333, 672
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 1 cut(s) 439
MalI GATC 3 cut(s) 645, 1002, 1118
MboI GATC 3 cut(s) 643, 1000, 1116
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 1 cut(s) 1253
MluCI AATT 5 cut(s) 290, 455, 593, 746, 795
MlyI GAGTC 3 cut(s) 884, 1174, 1271
MmeI TCCRAC 1 cut(s) 180
Mph1103I ATGCAT 1 cut(s) 1132
MroI TCCGGA 1 cut(s) 1258
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 2 cut(s) 798, 1113
MslI CAYNNNNRTG 1 cut(s) 884
MspI CCGG 1 cut(s) 1259
MspR9I CCNGG 3 cut(s) 740, 1044, 1171
MvaI CCWGG 3 cut(s) 740, 1044, 1171
MwoI GCNNNNNNNGC 2 cut(s) 563, 692
NdeII GATC 3 cut(s) 643, 1000, 1116
NlaIII CATG 2 cut(s) 236, 1123
NlaIV GGNNCC 1 cut(s) 1068
NmeAIII GCCGAG 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 1132
PciSI GCTCTTC 1 cut(s) 1002
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 3 cut(s) 415, 827, 1014
PkrI GCNGC 2 cut(s) 205, 975
PleI GAGTC 3 cut(s) 883, 1174, 1270
PpsI GAGTC 3 cut(s) 883, 1174, 1270
PpuMI RGGWCCY 1 cut(s) 1066
PsiI TTATAA 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 1066
Psp6I CCWGG 3 cut(s) 738, 1042, 1169
PspGI CCWGG 3 cut(s) 738, 1042, 1169
PspN4I GGNNCC 1 cut(s) 1068
PspPI GGNCC 2 cut(s) 420, 1066
PspPPI RGGWCCY 1 cut(s) 1066
PstNI CAGNNNCTG 1 cut(s) 1004
PsuI RGATCY 1 cut(s) 1000
RsaI GTAC 5 cut(s) 278, 923, 957, 1082, 1087
RsaNI GTAC 5 cut(s) 277, 922, 956, 1081, 1086
RseI CAYNNNNRTG 1 cut(s) 884
SapI GCTCTTC 1 cut(s) 1002
SaqAI TTAA 2 cut(s) 798, 1113
SatI GCNGC 2 cut(s) 204, 974
Sau3AI GATC 3 cut(s) 643, 1000, 1116
Sau96I GGNCC 2 cut(s) 420, 1066
ScaI AGTACT 1 cut(s) 1087
SchI GAGTC 3 cut(s) 884, 1174, 1271
ScrFI CCNGG 3 cut(s) 740, 1044, 1171
SduI GDGCHC 1 cut(s) 1253
SfaNI GCATC 4 cut(s) 415, 640, 665, 1117
SinI GGWCC 1 cut(s) 1066
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 4 cut(s) 20, 211, 521, 1214
SmoI CTYRAG 4 cut(s) 20, 211, 521, 1214
Sse9I AATT 5 cut(s) 290, 455, 593, 746, 795
SsiI CCGC 1 cut(s) 428
SspI AATATT 2 cut(s) 716, 1238
SspMI CTAG 2 cut(s) 333, 672
StyD4I CCNGG 3 cut(s) 738, 1042, 1169
StyI CCWWGG 3 cut(s) 332, 810, 1062
TaaI ACNGT 2 cut(s) 854, 1085
TaiI ACGT 1 cut(s) 552
TaqI TCGA 3 cut(s) 601, 777, 1125
TasI AATT 5 cut(s) 290, 455, 593, 746, 795
TatI WGTACW 3 cut(s) 921, 1080, 1085
TfiI GAWTC 3 cut(s) 415, 827, 1014
Tru1I TTAA 2 cut(s) 798, 1113
Tru9I TTAA 2 cut(s) 798, 1113
TscAI CASTG 3 cut(s) 697, 859, 1029
TseI GCWGC 2 cut(s) 203, 973
TspDTI ATGAA 8 cut(s) 17, 114, 461, 510, 822, 902, 1071, 1198
TspRI CASTG 3 cut(s) 697, 859, 1029
VpaK11BI GGWCC 1 cut(s) 1066
XagI CCTNNNNNAGG 1 cut(s) 809
XapI RAATTY 2 cut(s) 593, 746
XmaJI CCTAGG 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 2 cut(s) 333, 672
ZraI GACGTC 1 cut(s) 550
ZrmI AGTACT 1 cut(s) 1087
Zsp2I ATGCAT 1 cut(s) 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.