RLG00000020077

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018060
Physical Location & Seq
Forward (+)
60789953 .. 60790572
620 bp
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UTR
Exon/CDS
Intron
RLM00000020077

Sequence Viewer

Length: 552 bp
ATGGTTCCACGCCTTTACCACGTCACCATGAAGACAAATCTCGATGGCTTCTGGACTAAGGGTGAAATAAGAATGGAAGGTGTAATGCAATTTTATGTAAACGTTGAAAAGGAAGAAGGGAAGTTTGACAAACTCTGTGATCTCTACGATACCTTGTGCATCACTTCCCAGAGTGTCATCGTTGTTAATACAAGGCTCAAAGCGGAATGGCTTACTCAAAAGATGCGAAGCAAGGCGCACATCGTGTCTGCTATCCATGGCGACATGGACCGGTTCAATCGTGACAGGATTATGCGTGAGTTCCATTCTGGCACTTGCCCTGTTCTGATCACCAGTACCGATATGATGGCTTCTAATATCAATGCGGAGCAAGTATCTCTAGCCATAAATTATGATTTGCCTTCTCAACCAGAACAATACCGACGGATTGAAACGTTCGGAAGAACAGGTGTGGCCATTAACTTTGTGACCAGGGATGAGAAGAGAGTGCTGCACGATATCGAGAGGGTTTGTAATATGGACATCAGGGAGCTGCCATTTTCCACCAGCTAA

Protein Analysis

184

Amino Acids

21.27

Weight (kDa)

6.52

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 41 - 142 7.6e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018060)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g34110
rosa_laevigata RLG00000020077
rosa_multiflora Rmu_co8505185.1_g000001 Rmu_sc0003475.1_g000011 Rmu_sc0003475.1_g000013
rosa_roxburghii Rroxscaffold_2G00101340
rosa_rugosa Rorug02G0386100
rosa_samantha Rh2AG438500 Rh2CG425600 Rh2DG458600
rosa_wichuraiana Rw2G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 203, 365
AclI AACGTT 2 cut(s) 102, 434
AcoI YGGCCR 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 244
AfaI GTAC 1 cut(s) 337
AgeI ACCGGT 1 cut(s) 270
AgsI TTSAA 3 cut(s) 107, 277, 431
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 470
AluBI AGCT 2 cut(s) 532, 549
AluI AGCT 2 cut(s) 532, 549
AoxI GGCC 1 cut(s) 453
ApeKI GCWGC 2 cut(s) 490, 532
AsiGI ACCGGT 1 cut(s) 270
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 268
AsuHPI GGTGA 3 cut(s) 16, 74, 322
AvaII GGWCC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 455
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 477, 519
BccI CCATC 2 cut(s) 38, 340
BciT130I CCWGG 1 cut(s) 472
BclI TGATCA 1 cut(s) 327
BfaI CTAG 1 cut(s) 380
BisI GCNGC 2 cut(s) 491, 533
BlsI GCNGC 2 cut(s) 492, 534
Bme1390I CCNGG 1 cut(s) 472
Bme18I GGWCC 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 472
BmsI GCATC 2 cut(s) 168, 213
BpiI GAAGAC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 256, 471
BsaWI WCCGGW 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 1 cut(s) 333
BseBI CCWGG 1 cut(s) 472
BseDI CCNNGG 2 cut(s) 256, 471
BseGI GGATG 1 cut(s) 481
BseNI ACTGG 1 cut(s) 333
BseXI GCAGC 2 cut(s) 477, 519
BsgI GTGCAG 1 cut(s) 476
BshFI GGCC 1 cut(s) 455
BshTI ACCGGT 1 cut(s) 270
BsiSI CCGG 1 cut(s) 271
BsnI GGCC 1 cut(s) 455
Bsp143I GATC 2 cut(s) 139, 327
Bsp19I CCATGG 1 cut(s) 256
BspACI CCGC 2 cut(s) 203, 365
BspANI GGCC 1 cut(s) 455
BspLI GGNNCC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 270
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 2 cut(s) 256, 471
BssMI GATC 2 cut(s) 139, 327
BssT1I CCWWGG 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 476
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 481
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 2 cut(s) 142, 330
BstMBI GATC 2 cut(s) 139, 327
BstNI CCWGG 1 cut(s) 472
BstSCI CCNGG 1 cut(s) 470
BstV1I GCAGC 2 cut(s) 477, 519
BstV2I GAAGAC 1 cut(s) 38
BsuRI GGCC 1 cut(s) 455
BtgI CCRYGG 1 cut(s) 256
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 481
CfoI GCGC 1 cut(s) 238
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 336
CspAI ACCGGT 1 cut(s) 270
CviAII CATG 3 cut(s) 28, 257, 265
CviJI RGCY 8 cut(s) 48, 196, 211, 350, 383, 455, 532, 549
CviKI_1 RGCY 8 cut(s) 48, 196, 211, 350, 383, 455, 532, 549
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 1 cut(s) 57
DpnI GATC 2 cut(s) 141, 329
DpnII GATC 2 cut(s) 139, 327
DraIII CACNNNGTG 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 256
Eco32I GATATC 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 470
EcoRV GATATC 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 3 cut(s) 31, 260, 268
FaiI YATR 9 cut(s) 29, 96, 258, 266, 293, 344, 386, 393, 518
FatI CATG 3 cut(s) 27, 256, 264
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 491, 533
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 2 cut(s) 491, 533
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 237
GluI GCNGC 2 cut(s) 491, 533
HaeIII GGCC 1 cut(s) 455
HapII CCGG 1 cut(s) 271
HhaI GCGC 1 cut(s) 238
Hin1II CATG 3 cut(s) 31, 260, 268
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HpaII CCGG 1 cut(s) 271
HphI GGTGA 3 cut(s) 16, 74, 322
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 327, 440
Hpy188III TCNNGA 4 cut(s) 41, 52, 281, 502
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 3 cut(s) 71, 110, 411
HpyCH4IV ACGT 3 cut(s) 21, 102, 434
HpyCH4V TGCA 3 cut(s) 88, 159, 493
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 3 cut(s) 21, 102, 434
Hsp92II CATG 3 cut(s) 31, 260, 268
HspAI GCGC 1 cut(s) 236
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 2 cut(s) 139, 327
LmnI GCTCC 2 cut(s) 367, 529
Lsp1109I GCAGC 2 cut(s) 477, 519
LweI GCATC 2 cut(s) 168, 213
MaeI CTAG 1 cut(s) 380
MaeII ACGT 3 cut(s) 21, 102, 434
MaeIII GTNAC 3 cut(s) 22, 281, 466
MalI GATC 2 cut(s) 141, 329
MboI GATC 2 cut(s) 139, 327
MboII GAAGA 4 cut(s) 43, 125, 453, 493
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 2 cut(s) 89, 388
MluNI TGGCCA 1 cut(s) 455
MnlI CCTC 1 cut(s) 498
Mox20I TGGCCA 1 cut(s) 455
MscI TGGCCA 1 cut(s) 455
MseI TTAA 2 cut(s) 186, 459
Msp20I TGGCCA 1 cut(s) 455
MspI CCGG 1 cut(s) 271
MspR9I CCNGG 1 cut(s) 472
MvaI CCWGG 1 cut(s) 472
NcoI CCATGG 1 cut(s) 256
NdeII GATC 2 cut(s) 139, 327
NlaIII CATG 3 cut(s) 31, 260, 268
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 22, 281, 466
PinAI ACCGGT 1 cut(s) 270
PkrI GCNGC 2 cut(s) 492, 534
Psp1406I AACGTT 2 cut(s) 102, 434
Psp6I CCWGG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 470
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 268
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 186, 459
SatI GCNGC 2 cut(s) 491, 533
Sau3AI GATC 2 cut(s) 139, 327
Sau96I GGNCC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 472
SetI ASST 8 cut(s) 24, 82, 105, 155, 437, 451, 534, 551
SfaNI GCATC 2 cut(s) 168, 213
SinI GGWCC 1 cut(s) 268
Sse9I AATT 2 cut(s) 89, 388
SsiI CCGC 2 cut(s) 203, 365
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 470
StyI CCWWGG 1 cut(s) 256
TaiI ACGT 3 cut(s) 24, 105, 437
TaqI TCGA 2 cut(s) 42, 501
TasI AATT 2 cut(s) 89, 388
Tru1I TTAA 2 cut(s) 186, 459
Tru9I TTAA 2 cut(s) 186, 459
TseFI GTSAC 3 cut(s) 22, 281, 466
TseI GCWGC 2 cut(s) 490, 532
Tsp45I GTSAC 3 cut(s) 22, 281, 466
TspDTI ATGAA 1 cut(s) 44
TspGWI ACGGA 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 268
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.