RLG00000020393

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015095
Physical Location & Seq
Reverse (-)
64549898 .. 64552206
2309 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020393

Sequence Viewer

Length: 531 bp
ATGAAGGAAATGGAGCTGTACCGGTCGGAAATCGGAAAGCCGGTGATTGTTCTCATATGCGGTACCTTGGTCTACTACCACTGCGCCCATCGGAACTCGAGCCTCGTTTCTCTCGTCTCCGATGTCCTCATCGTCCTCCTCTGCTCTCTCGCCATTCTCGGCCTTCTCTTCCGCCAAATGAACATCTCGGTACCTGTGGATCCACTTGAGTGGCAGATTTCGCAGGACACTGCGAATAGCATTGTCGCGTGGATGGCTAATACTGTAGGAGCGGCTGAGTCTGTGTTGAGAGTTGCGGCCACGGGGCATGACAAGAGGTTGTTTTTCAAGGTAGTGTTTTGTCTTTACGTGCTATCAGTTTTGGGACGGTTGGTTTCAGGTCTTACAGTTGCCTATGCTGGATTATGCTTATTTTGCCTTTATATGTTTGCGGAGAGCAGTCAGTCGATCAGTTCATGTTTGTCTCGTTTTCTGGGGAGAAACAATGGCCTTACAGCGGAAGATACTGAAGAGCAGGAGGATACTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

177

Amino Acids

19.4

Weight (kDa)

5.31

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 15 - 138 2.8e-17 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015095)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16825 AT1G78895
fragaria_vesca FvH4_6g36900 FvH4_6g36900
malus_domestica MD09G1166000.v1.1 MD17G1161700.v1.1
prunus_persica Prupe.3G023400_v2.0.a1
pyrus_communis pycom17g15490
rosa_chinensis RchiOBHm_Chr2g0149331
rosa_laevigata RLG00000020393
rosa_multiflora Rmu_sc0000873.1_g000016
rosa_roxburghii Rroxscaffold_2G00098210
rosa_rugosa Rorug02G0410600
rosa_samantha Rh2AG471300 Rh2CG457200
rosa_wichuraiana Rw2G038340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 62, 190
AccB1I GGYRCC 2 cut(s) 62, 190
AccBSI CCGCTC 1 cut(s) 272
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 248
AciI CCGC 6 cut(s) 60, 172, 272, 296, 431, 497
AclWI GGATC 2 cut(s) 194, 207
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 528
AfaI GTAC 3 cut(s) 20, 64, 192
AfiI CCNNNNNNNGG 1 cut(s) 496
AgeI ACCGGT 1 cut(s) 21
AgsI TTSAA 1 cut(s) 328
AleI CACNNNNGTG 1 cut(s) 208
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
Alw26I GTCTC 2 cut(s) 121, 468
AlwI GGATC 2 cut(s) 194, 207
Ama87I CYCGRG 1 cut(s) 97
AoxI GGCC 3 cut(s) 160, 297, 487
AsiGI ACCGGT 1 cut(s) 21
Asp718I GGTACC 2 cut(s) 62, 190
AspLEI GCGC 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 55
AvaI CYCGRG 1 cut(s) 97
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BamHI GGATCC 1 cut(s) 199
BanI GGYRCC 2 cut(s) 62, 190
BccI CCATC 2 cut(s) 96, 247
BciVI GTATCC 1 cut(s) 514
BcoDI GTCTC 2 cut(s) 121, 468
BfmI CTRYAG 1 cut(s) 264
BfuI GTATCC 1 cut(s) 514
BisI GCNGC 2 cut(s) 273, 297
BlsI GCNGC 2 cut(s) 274, 298
BmeT110I CYCGRG 1 cut(s) 97
BmiI GGNNCC 3 cut(s) 64, 192, 201
BpuEI CTTGAG 1 cut(s) 227
BsaAI YACGTR 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 66, 300
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 496
Bse118I RCCGGY 2 cut(s) 21, 40
BseDI CCNNGG 2 cut(s) 66, 300
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMII CTCAG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 3 cut(s) 162, 299, 489
BshNI GGYRCC 2 cut(s) 62, 190
BshTI ACCGGT 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 2 cut(s) 22, 41
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 496
BsmAI GTCTC 2 cut(s) 121, 468
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 3 cut(s) 162, 299, 489
BsoBI CYCGRG 1 cut(s) 97
Bsp143I GATC 2 cut(s) 199, 447
BspACI CCGC 6 cut(s) 60, 172, 272, 296, 431, 497
BspANI GGCC 3 cut(s) 162, 299, 489
BspCNI CTCAG 1 cut(s) 268
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 64, 192, 201
BspPI GGATC 2 cut(s) 194, 207
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 2 cut(s) 62, 190
BsrBI CCGCTC 1 cut(s) 272
BsrFI RCCGGY 2 cut(s) 21, 40
BssAI RCCGGY 2 cut(s) 21, 40
BssECI CCNNGG 2 cut(s) 66, 300
BssMI GATC 2 cut(s) 199, 447
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 265, 369, 388
Bst6I CTCTTC 2 cut(s) 173, 504
BstBAI YACGTR 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 2 cut(s) 202, 450
BstMAI GTCTC 2 cut(s) 121, 468
BstMBI GATC 2 cut(s) 199, 447
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 220, 254, 414
BstSFI CTRYAG 1 cut(s) 264
BstUI CGCG 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 210
BstYI RGATCY 1 cut(s) 199
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 3 cut(s) 162, 299, 489
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 1 cut(s) 258
BtsI GCAGTG 2 cut(s) 79, 228
BtsIMutI CAGTG 2 cut(s) 79, 228
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 2 cut(s) 21, 40
Csp6I GTAC 3 cut(s) 19, 63, 191
CspAI ACCGGT 1 cut(s) 21
CviAII CATG 2 cut(s) 308, 456
CviJI RGCY 8 cut(s) 16, 40, 102, 162, 257, 275, 299, 489
CviKI_1 RGCY 8 cut(s) 16, 40, 102, 162, 257, 275, 299, 489
CviQI GTAC 3 cut(s) 19, 63, 191
DdeI CTNAG 1 cut(s) 276
DpnI GATC 2 cut(s) 201, 449
DpnII GATC 2 cut(s) 199, 447
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 173, 504
EarI CTCTTC 2 cut(s) 173, 504
EciI GGCGGA 1 cut(s) 161
Eco130I CCWWGG 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 2 cut(s) 311, 459
FaiI YATR 9 cut(s) 56, 58, 309, 396, 406, 423, 425, 457, 527
FaqI GGGAC 1 cut(s) 378
FatI CATG 2 cut(s) 307, 455
FauNDI CATATG 1 cut(s) 56
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 2 cut(s) 273, 297
FokI GGATG 1 cut(s) 265
Fsp4HI GCNGC 2 cut(s) 273, 297
GlaI GCGC 1 cut(s) 85
GluI GCNGC 2 cut(s) 273, 297
HaeIII GGCC 3 cut(s) 162, 299, 489
HapII CCGG 2 cut(s) 22, 41
HhaI GCGC 1 cut(s) 86
Hin1II CATG 2 cut(s) 311, 459
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 1 cut(s) 278
HpaII CCGG 2 cut(s) 22, 41
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 4 cut(s) 28, 35, 93, 121
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 173
HpyCH4III ACNGT 3 cut(s) 265, 369, 388
HpyCH4IV ACGT 1 cut(s) 348
HpyF10VI GCNNNNNNNGC 3 cut(s) 220, 254, 414
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 348
Hsp92II CATG 2 cut(s) 311, 459
HspAI GCGC 1 cut(s) 84
KpnI GGTACC 2 cut(s) 66, 194
Kzo9I GATC 2 cut(s) 199, 447
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 2 cut(s) 13, 269
LpnPI CCDG 8 cut(s) 35, 54, 207, 209, 363, 384, 458, 500
MaeII ACGT 1 cut(s) 348
MalI GATC 2 cut(s) 201, 449
MbiI CCGCTC 1 cut(s) 272
MboI GATC 2 cut(s) 199, 447
MboII GAAGA 3 cut(s) 160, 512, 521
MflI RGATCY 1 cut(s) 199
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 1 cut(s) 6
MnlI CCTC 6 cut(s) 113, 137, 146, 149, 309, 511
MslI CAYNNNNRTG 1 cut(s) 208
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 2 cut(s) 22, 41
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 3 cut(s) 220, 254, 414
NdeI CATATG 1 cut(s) 56
NdeII GATC 2 cut(s) 199, 447
NlaIII CATG 2 cut(s) 311, 459
NlaIV GGNNCC 3 cut(s) 64, 192, 201
NmeAIII GCCGAG 1 cut(s) 138
OliI CACNNNNGTG 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 97
PciSI GCTCTTC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PinAI ACCGGT 1 cut(s) 21
PkrI GCNGC 2 cut(s) 274, 298
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 349
PspN4I GGNNCC 3 cut(s) 64, 192, 201
PspXI VCTCGAGB 1 cut(s) 97
PsuI RGATCY 1 cut(s) 199
RsaI GTAC 3 cut(s) 20, 64, 192
RsaNI GTAC 3 cut(s) 19, 63, 191
RseI CAYNNNNRTG 1 cut(s) 208
SapI GCTCTTC 1 cut(s) 504
SatI GCNGC 2 cut(s) 273, 297
Sau3AI GATC 2 cut(s) 199, 447
SchI GAGTC 1 cut(s) 287
SetI ASST 7 cut(s) 18, 68, 196, 320, 333, 351, 382
SfcI CTRYAG 1 cut(s) 264
Sfr274I CTCGAG 1 cut(s) 97
SlaI CTCGAG 1 cut(s) 97
SmiMI CAYNNNNRTG 1 cut(s) 208
SmlI CTYRAG 2 cut(s) 97, 206
SmoI CTYRAG 2 cut(s) 97, 206
SsiI CCGC 6 cut(s) 60, 172, 272, 296, 431, 497
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 3 cut(s) 265, 369, 388
TaiI ACGT 1 cut(s) 351
TaqI TCGA 2 cut(s) 98, 446
TauI GCSGC 2 cut(s) 275, 299
TscAI CASTG 2 cut(s) 86, 235
TspDTI ATGAA 3 cut(s) 17, 194, 444
TspRI CASTG 2 cut(s) 86, 235
XhoI CTCGAG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.