RLG00000020540

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018108
Physical Location & Seq
Forward (+)
66088571 .. 66089468
898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020540

Sequence Viewer

Length: 495 bp
ATGGAGCTCTCCGAGTCACATTCTTCCATTAATCTGGAAAATGTTGGTTTAAAAGTGGCCAATGATGATGACCTCCGAAACTCTCACATTTCAGAGAGATTCGAGTCGGCCGGAGCGAGCTTCATCGCTCACAGAGACAGTGAAGAGCTCTTGCGTCAGGTGAAACTCCAGGCATCTTCGCCGGAAGCCTTGGGAGGAGCTGCGGCGGAGTTCGCGACGGCACTGCTACCCACTGACGGCGCAAGGTTGGGCGTAAAGTTGAATCCAGGTGTTGACTCCATTGAAGACTCGGTCATCCTCATGGAAGAAAAGGACTTGGGAGCAAACAGTGAAATCATCAATCTACGAAGAGAAAGAGCTATAGACCTGGGATCATGTGAAGATATGCGTTACATATTGCCTAACCATACCAGTCTCACTAACCAAGTTTACCTCAATCCACAAAAATGTTCTGCTTTAGGGTTGACAAATGAAGGCACCACTACTGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

165

Amino Acids

17.81

Weight (kDa)

4.69

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018108)

Species Orthologous Gene IDs
malus_domestica MD08G1122100.v1.1
rosa_laevigata RLG00000020540
rosa_multiflora Rmu_sc0001102.1_g000001
rosa_roxburghii Rroxscaffold_2G00096540 Rroxscaffold_2G00096580
rosa_samantha Rh2BG498000 Rh2BG498400 Rh2DG508900 Rh2DG509200
rosa_wichuraiana Rw2G039820 Rw2G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AccII CGCG 1 cut(s) 215
AciI CCGC 2 cut(s) 203, 206
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 2 cut(s) 57, 108
AfiI CCNNNNNNNGG 1 cut(s) 236
AgsI TTSAA 2 cut(s) 262, 284
AjnI CCWGG 3 cut(s) 168, 265, 366
AluBI AGCT 5 cut(s) 7, 120, 148, 200, 359
AluI AGCT 5 cut(s) 7, 120, 148, 200, 359
Alw21I GWGCWC 2 cut(s) 9, 150
Alw26I GTCTC 2 cut(s) 129, 419
AlwI GGATC 1 cut(s) 379
AoxI GGCC 2 cut(s) 57, 108
ApeKI GCWGC 1 cut(s) 200
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 172
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 1 cut(s) 476
BanII GRGCYC 2 cut(s) 9, 150
BbsI GAAGAC 1 cut(s) 291
Bbv12I GWGCWC 2 cut(s) 9, 150
BbvI GCAGC 1 cut(s) 187
BceAI ACGGC 2 cut(s) 234, 253
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 3 cut(s) 170, 267, 368
BcoDI GTCTC 2 cut(s) 129, 419
BfmI CTRYAG 1 cut(s) 360
BisI GCNGC 2 cut(s) 201, 204
BlsI GCNGC 2 cut(s) 202, 205
Bme1390I CCNGG 3 cut(s) 170, 267, 368
BmiI GGNNCC 1 cut(s) 478
BmrFI CCNGG 3 cut(s) 170, 267, 368
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 291
BpmI CTGGAG 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 189, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 3 cut(s) 170, 267, 368
BseDI CCNNGG 2 cut(s) 189, 367
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 215
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 59, 110
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 9, 150
BsiSI CCGG 2 cut(s) 111, 182
BslI CCNNNNNNNGG 1 cut(s) 236
BsmAI GTCTC 2 cut(s) 129, 419
BsnI GGCC 2 cut(s) 59, 110
Bsp1286I GDGCHC 2 cut(s) 9, 150
Bsp143I GATC 1 cut(s) 371
Bsp68I TCGCGA 1 cut(s) 215
BspACI CCGC 2 cut(s) 203, 206
BspANI GGCC 2 cut(s) 59, 110
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 478
BspPI GGATC 1 cut(s) 379
BspQI GCTCTTC 1 cut(s) 138
BspT107I GGYRCC 1 cut(s) 476
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 2 cut(s) 189, 367
BssMI GATC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 3 cut(s) 170, 267, 368
Bst4CI ACNGT 2 cut(s) 140, 329
Bst6I CTCTTC 2 cut(s) 138, 343
BstC8I GCNNGC 1 cut(s) 118
BstF5I GGATG 1 cut(s) 294
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 1 cut(s) 374
BstMAI GTCTC 2 cut(s) 129, 419
BstMBI GATC 1 cut(s) 371
BstMCI CGRYCG 1 cut(s) 111
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 3 cut(s) 170, 267, 368
BstSCI CCNGG 3 cut(s) 168, 265, 366
BstSFI CTRYAG 1 cut(s) 360
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 187
BstV2I GAAGAC 1 cut(s) 291
BstXI CCANNNNNNTGG 1 cut(s) 34
BstZI CGGCCG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 59, 110
BtgZI GCGATG 1 cut(s) 109
BtsCI GGATG 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 221
BtsIMutI CAGTG 4 cut(s) 145, 221, 231, 334
BtuMI TCGCGA 1 cut(s) 215
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 1 cut(s) 242
CseI GACGC 1 cut(s) 143
CviAII CATG 2 cut(s) 301, 375
CviJI RGCY 8 cut(s) 7, 59, 110, 120, 148, 188, 200, 359
CviKI_1 RGCY 8 cut(s) 7, 59, 110, 120, 148, 188, 200, 359
DpnI GATC 1 cut(s) 373
DpnII GATC 1 cut(s) 371
DraI TTTAAA 1 cut(s) 51
EaeI YGGCCR 2 cut(s) 57, 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 138, 343
EarI CTCTTC 2 cut(s) 138, 343
EciI GGCGGA 1 cut(s) 221
Ecl136II GAGCTC 2 cut(s) 7, 148
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 2 cut(s) 9, 150
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 2 cut(s) 7, 148
EcoICRI GAGCTC 2 cut(s) 7, 148
EcoRII CCWGG 3 cut(s) 168, 265, 366
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 2 cut(s) 9, 150
ErhI CCWWGG 1 cut(s) 189
FaeI CATG 2 cut(s) 304, 378
FaiI YATR 6 cut(s) 302, 362, 376, 386, 395, 408
FatI CATG 2 cut(s) 300, 374
Fnu4HI GCNGC 2 cut(s) 201, 204
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 2 cut(s) 9, 150
Fsp4HI GCNGC 2 cut(s) 201, 204
GlaI GCGC 1 cut(s) 241
GluI GCNGC 2 cut(s) 201, 204
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 2 cut(s) 59, 110
HapII CCGG 2 cut(s) 111, 182
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 242
Hin1II CATG 2 cut(s) 304, 378
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 2 cut(s) 274, 465
HindII GTYRAC 2 cut(s) 274, 465
HinfI GANTC 6 cut(s) 14, 99, 104, 262, 275, 287
HpaII CCGG 2 cut(s) 111, 182
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 3 cut(s) 274, 430, 465
Hpy188I TCNGA 3 cut(s) 13, 77, 94
Hpy188III TCNNGA 2 cut(s) 35, 214
Hpy8I GTNNAC 3 cut(s) 274, 430, 465
Hpy99I CGWCG 1 cut(s) 220
HpyAV CCTTC 1 cut(s) 467
HpyCH4III ACNGT 2 cut(s) 140, 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
Hsp92II CATG 2 cut(s) 304, 378
HspAI GCGC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 371
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 4 cut(s) 4, 113, 197, 320
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 15, 389
MalI GATC 1 cut(s) 373
MboI GATC 1 cut(s) 371
MboII GAAGA 7 cut(s) 15, 155, 168, 296, 317, 360, 392
MhlI GDGCHC 2 cut(s) 9, 150
MlsI TGGCCA 1 cut(s) 59
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 4 cut(s) 23, 113, 269, 281
MnlI CCTC 4 cut(s) 83, 188, 308, 443
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 30, 50
MslI CAYNNNNRTG 2 cut(s) 299, 445
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 2 cut(s) 111, 182
MspR9I CCNGG 3 cut(s) 170, 267, 368
MvaI CCWGG 3 cut(s) 170, 267, 368
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 1 cut(s) 212
NdeII GATC 1 cut(s) 371
NlaIII CATG 2 cut(s) 304, 378
NlaIV GGNNCC 1 cut(s) 478
NmuCI GTSAC 1 cut(s) 15
NruI TCGCGA 1 cut(s) 215
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 113
PfeI GAWTC 2 cut(s) 99, 262
PflFI GACNNNGTC 1 cut(s) 290
PkrI GCNGC 2 cut(s) 202, 205
PleI GAGTC 4 cut(s) 22, 112, 269, 281
PpsI GAGTC 4 cut(s) 22, 112, 269, 281
PshBI ATTAAT 1 cut(s) 30
Psp124BI GAGCTC 2 cut(s) 9, 150
Psp6I CCWGG 3 cut(s) 168, 265, 366
PspGI CCWGG 3 cut(s) 168, 265, 366
PspN4I GGNNCC 1 cut(s) 478
PsyI GACNNNGTC 1 cut(s) 290
RruI TCGCGA 1 cut(s) 215
RseI CAYNNNNRTG 2 cut(s) 299, 445
SacI GAGCTC 2 cut(s) 9, 150
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 30, 50
SatI GCNGC 2 cut(s) 201, 204
Sau3AI GATC 1 cut(s) 371
SchI GAGTC 4 cut(s) 23, 113, 269, 281
ScrFI CCNGG 3 cut(s) 170, 267, 368
SduI GDGCHC 2 cut(s) 9, 150
SfaNI GCATC 1 cut(s) 182
SfcI CTRYAG 1 cut(s) 360
SmiMI CAYNNNNRTG 2 cut(s) 299, 445
SsiI CCGC 2 cut(s) 203, 206
SstI GAGCTC 2 cut(s) 9, 150
StyD4I CCNGG 3 cut(s) 168, 265, 366
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 2 cut(s) 140, 329
TaqI TCGA 1 cut(s) 102
TaqII GACCGA 1 cut(s) 280
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 2 cut(s) 99, 262
Tru1I TTAA 2 cut(s) 30, 50
Tru9I TTAA 2 cut(s) 30, 50
TscAI CASTG 4 cut(s) 145, 228, 238, 334
TseFI GTSAC 1 cut(s) 15
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 2 cut(s) 112, 486
TspRI CASTG 4 cut(s) 145, 228, 238, 334
Tth111I GACNNNGTC 1 cut(s) 290
VspI ATTAAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.