Rroxscaffold_2G00096540

OST-HTH/LOTUS domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018108
Physical Location & Seq
Reverse (-)
17781793 .. 17786099
4307 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00096540.1

Sequence Viewer

Length: 1611 bp
ATGATGATGACCTTCGAAACTCTCACATTTCAGACCTCCGAAACTCTCACCTTTCAGAGAGATTCGAGTCTGGCCGGAGCGAGCTGCGGAGCACTCCAGGCATCTTCGCCGGAAACCTTGGGCGGAGCTGCGGCGAAGTGCGCGACGGCACTGGTACCCACTGACGGCGCAAGGTTGGGTGTAATGTTGAATCCAGATGTTGACGCCGTTGAAGACTCGGTCATCCTCATGGAAGAAAAGGACCTGACCTTTGGGGCAAACAGTGAAATCATCAATCTACGAAGAGAAAGAGCTACAGACGTGGGATCATGTGAAGATATGCGTTGCATATTGCCAAACCATACCAGTCTCACTAACCAAGTTTACCTCAATCCACAAAAATGTTCTGGTTTAGGGTTGACAAATGAAGGCACCACTACTGAAAGGAATGCTAGTATTTCAAGCCCCACAGAAGAGGGCTCTCATCGAGGATGTTTAAGAGGAGCCAAGTGCTTCTATGCACATTGTGAAGAGGAGTCTTGGAAAGTGAAGCACAGAAAACATTGGAGTCATTTTTCTGCTGCTAAACAACTCAAAAGCAAAATCTCTGTAGGAGGACTTCCTCCTTCAGTGGACTCCGATATTTTGGACAAAACTAAAGGTACTACCAATGTTGTAGCGAGTTTAGAGAATGAAAAGAAAATCATCGTTGGAAGTGACTCCAGATTGACCTACGAAGGAACTATAAGAACAGATGTGCTAAAAAAATTTGAAGTGATAACTTCAAATATAATTGCCACAATGGTTGGAAGTGCGGGCGGATGCGAAAAGATCTGGAAACGCATGCAAGAATACTGTAATTCGGAAGGAGTAGAGCCAAAAACAATTTCTTCCATATCCAAATTTGGTAGTGAGATACCTAGGAAAGTTGATGATTTTCTGTCATCTGAATGTTTTGTCATCGGGGTTGAAAATGATAAACCGACCGTATATCATCTTACAGACTTGTATGATGAAGAATTAAAAAGGCGTGTAAAGGATGCGACGAAAGTTTCTGATACTTTTGCTGCACTAGGATCTGGAAGTGACATTGCTCTTAATGCATGGAATGAAGCATTGAAGGGACGTAGCCTTCACCTAGAGGAAACATTTGAAACAATCTCGTTTGGACTACTTAAGGCAGCATTGTACAATCCCGATACTGGTGGAATATTACGAGTGATACAGTTATGCTCAACTGGAATTGAGATAGAGTCCAACAAAACTGCTGTTGAACTTTATCTACAATTCTATGATCGCTTTCAAGAGTTGGAGAATAGATCATTTTTTCTATTTTACATTCCGAGTATTAAGAAAAATGATCTACGCCAATGGCTTCGGCAATCAGGAACCATAGAGGATTTTGGTCATCCATTGACACAACTTTGCGGTCGTTATTATGTGCACCGCGTTTTATTCAAGGATCATGTGGCTTCCACACATCTAGGAGCAAAACCTATTCCTAAGTCGAAGTGGCCTGAGTTTACTGATTCACCAAGTGCGTTAAGGTTCATTATTATTGCAAGCAAGAAAGTGTTTGTTGCAAGACAATCAGCAAAACTTTTGAGCTCACTGAAACTTGCAGATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 OST-HTH RRM_1

Protein Analysis

536

Amino Acids

59.28

Weight (kDa)

6.35

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 222 - 387 1.2e-07 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018108)

Species Orthologous Gene IDs
malus_domestica MD08G1122100.v1.1
rosa_laevigata RLG00000020540
rosa_multiflora Rmu_sc0001102.1_g000001
rosa_roxburghii Rroxscaffold_2G00096540 Rroxscaffold_2G00096580
rosa_samantha Rh2BG498000 Rh2BG498400 Rh2DG508900 Rh2DG509200
rosa_wichuraiana Rw2G039820 Rw2G040250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 154
AccB1I GGYRCC 2 cut(s) 154, 410
AccII CGCG 2 cut(s) 143, 1428
AciI CCGC 7 cut(s) 87, 123, 131, 794, 798, 1407, 1426
AclWI GGATC 3 cut(s) 313, 1063, 1449
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 2 cut(s) 746, 881
AcuI CTGAAG 1 cut(s) 591
AcyI GRCGYC 1 cut(s) 204
AdeI CACNNNGTG 2 cut(s) 506, 1517
AfaI GTAC 3 cut(s) 156, 643, 1169
AfiI CCNNNNNNNGG 2 cut(s) 164, 1181
AflII CTTAAG 1 cut(s) 1154
AjiI CACGTC 1 cut(s) 301
AjnI CCWGG 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 850, 882
AluBI AGCT 4 cut(s) 84, 128, 293, 1587
AluI AGCT 4 cut(s) 84, 128, 293, 1587
Alw21I GWGCWC 3 cut(s) 94, 1425, 1589
Alw26I GTCTC 1 cut(s) 353
Alw44I GTGCAC 1 cut(s) 1421
AlwI GGATC 3 cut(s) 313, 1063, 1449
AoxI GGCC 2 cut(s) 72, 1493
ApaLI GTGCAC 1 cut(s) 1421
ApeKI GCWGC 5 cut(s) 84, 128, 560, 1046, 1160
ApoI RAATTY 2 cut(s) 746, 881
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
Asp718I GGTACC 1 cut(s) 154
AspA2I CCTAGG 1 cut(s) 899
AspLEI GCGC 2 cut(s) 143, 170
AspS9I GGNCC 1 cut(s) 241
AsuHPI GGTGA 3 cut(s) 40, 1106, 1503
AsuII TTCGAA 1 cut(s) 15
AvaII GGWCC 1 cut(s) 241
AvrII CCTAGG 1 cut(s) 899
BaeGI GKGCMC 1 cut(s) 1425
BanI GGYRCC 2 cut(s) 154, 410
BanII GRGCYC 2 cut(s) 461, 1589
BarI GAAGNNNNNNTAC 2 cut(s) 582, 614
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 3 cut(s) 94, 1425, 1589
BbvI GCAGC 5 cut(s) 71, 115, 547, 1033, 1172
BceAI ACGGC 3 cut(s) 162, 181, 191
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 5 cut(s) 432, 900, 1052, 1118, 1463
BfmI CTRYAG 2 cut(s) 294, 588
BfrI CTTAAG 1 cut(s) 1154
BglII AGATCT 1 cut(s) 810
BisI GCNGC 6 cut(s) 85, 129, 132, 561, 1047, 1161
BlnI CCTAGG 1 cut(s) 899
BlsI GCNGC 6 cut(s) 86, 130, 133, 562, 1048, 1162
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 301
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 4 cut(s) 156, 412, 484, 1369
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 3 cut(s) 110, 791, 1009
BpiI GAAGAC 1 cut(s) 219
BpmI CTGGAG 2 cut(s) 80, 685
Bpu14I TTCGAA 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 117, 899
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 1181
Bse1I ACTGG 4 cut(s) 156, 345, 1186, 1222
Bse3DI GCAATG 1 cut(s) 1068
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 117, 899
BseGI GGATG 5 cut(s) 222, 476, 806, 1024, 1387
BseLI CCNNNNNNNGG 2 cut(s) 164, 1181
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 1 cut(s) 1488
BseNI ACTGG 4 cut(s) 156, 345, 1186, 1222
BseRI GAGGAG 2 cut(s) 495, 527
BseSI GKGCMC 1 cut(s) 1425
BseXI GCAGC 5 cut(s) 71, 115, 547, 1033, 1172
BsgI GTGCAG 1 cut(s) 1032
Bsh1236I CGCG 2 cut(s) 143, 1428
Bsh1285I CGRYCG 2 cut(s) 966, 1411
BshFI GGCC 2 cut(s) 74, 1495
BshNI GGYRCC 2 cut(s) 154, 410
BsiEI CGRYCG 2 cut(s) 966, 1411
BsiHKAI GWGCWC 3 cut(s) 94, 1425, 1589
BsiSI CCGG 2 cut(s) 75, 110
BslFI GGGAC 1 cut(s) 1116
BslI CCNNNNNNNGG 2 cut(s) 164, 1181
BsmAI GTCTC 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 1116
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 2 cut(s) 74, 1495
Bsp119I TTCGAA 1 cut(s) 15
Bsp1286I GDGCHC 4 cut(s) 94, 461, 1425, 1589
Bsp1407I TGTACA 1 cut(s) 1167
Bsp143I GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
BspACI CCGC 7 cut(s) 87, 123, 131, 794, 798, 1407, 1426
BspANI GGCC 2 cut(s) 74, 1495
BspCNI CTCAG 1 cut(s) 1489
BspFNI CGCG 2 cut(s) 143, 1428
BspLI GGNNCC 4 cut(s) 156, 412, 484, 1369
BspPI GGATC 3 cut(s) 313, 1063, 1449
BspT104I TTCGAA 1 cut(s) 15
BspT107I GGYRCC 2 cut(s) 154, 410
BspTI CTTAAG 1 cut(s) 1154
BsrDI GCAATG 1 cut(s) 1068
BsrGI TGTACA 1 cut(s) 1167
BsrI ACTGG 4 cut(s) 156, 345, 1186, 1222
BssECI CCNNGG 2 cut(s) 117, 899
BssMI GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 2 cut(s) 117, 899
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 4 cut(s) 263, 836, 967, 1206
Bst6I CTCTTC 3 cut(s) 277, 447, 504
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 1167
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 4 cut(s) 82, 796, 824, 1543
BstDEI CTNAG 2 cut(s) 1482, 1497
BstF5I GGATG 5 cut(s) 222, 476, 806, 1024, 1387
BstFNI CGCG 2 cut(s) 143, 1428
BstHHI GCGC 2 cut(s) 143, 170
BstKTI GATC 7 cut(s) 308, 813, 1058, 1276, 1301, 1342, 1444
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
BstMCI CGRYCG 2 cut(s) 966, 1411
BstMWI GCNNNNNNNGC 3 cut(s) 98, 140, 1079
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 826
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 2 cut(s) 294, 588
BstSLI GKGCMC 1 cut(s) 1425
BstUI CGCG 2 cut(s) 143, 1428
BstV1I GCAGC 5 cut(s) 71, 115, 547, 1033, 1172
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 2 cut(s) 810, 1055
BstYI RGATCY 2 cut(s) 810, 1055
BsuRI GGCC 2 cut(s) 74, 1495
BtrI CACGTC 1 cut(s) 301
BtsCI GGATG 5 cut(s) 222, 476, 806, 1024, 1387
BtsIMutI CAGTG 5 cut(s) 149, 159, 268, 615, 1589
Cac8I GCNNGC 4 cut(s) 82, 796, 824, 1543
CfoI GCGC 2 cut(s) 143, 170
Cfr13I GGNCC 1 cut(s) 241
CseI GACGC 1 cut(s) 212
Csp6I GTAC 3 cut(s) 155, 642, 1168
CspCI CAANNNNNGTGG 2 cut(s) 766, 801
CviAII CATG 5 cut(s) 229, 309, 823, 1083, 1445
CviQI GTAC 3 cut(s) 155, 642, 1168
DdeI CTNAG 2 cut(s) 1482, 1497
DpnI GATC 7 cut(s) 307, 812, 1057, 1275, 1300, 1341, 1443
DpnII GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
DraIII CACNNNGTG 2 cut(s) 506, 1517
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 3 cut(s) 277, 447, 504
EarI CTCTTC 3 cut(s) 277, 447, 504
EciI GGCGGA 2 cut(s) 138, 813
Ecl136II GAGCTC 1 cut(s) 1587
Eco130I CCWWGG 2 cut(s) 117, 899
Eco24I GRGCYC 2 cut(s) 461, 1589
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 1587
Eco57I CTGAAG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 1587
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 117, 899
EcoT22I ATGCAT 1 cut(s) 1084
EcoT38I GRGCYC 2 cut(s) 461, 1589
ErhI CCWWGG 2 cut(s) 117, 899
FaeI CATG 5 cut(s) 232, 312, 826, 1086, 1448
FaqI GGGAC 1 cut(s) 1116
FatI CATG 5 cut(s) 228, 308, 822, 1082, 1444
FauI CCCGC 1 cut(s) 787
Fnu4HI GCNGC 6 cut(s) 85, 129, 132, 561, 1047, 1161
FokI GGATG 5 cut(s) 209, 483, 813, 1031, 1374
FriOI GRGCYC 2 cut(s) 461, 1589
Fsp4HI GCNGC 6 cut(s) 85, 129, 132, 561, 1047, 1161
FspBI CTAG 5 cut(s) 432, 900, 1052, 1118, 1463
GlaI GCGC 2 cut(s) 142, 169
GluI GCNGC 6 cut(s) 85, 129, 132, 561, 1047, 1161
GsuI CTGGAG 2 cut(s) 80, 685
HaeIII GGCC 2 cut(s) 74, 1495
HapII CCGG 2 cut(s) 75, 110
HgaI GACGC 1 cut(s) 212
HhaI GCGC 2 cut(s) 143, 170
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 5 cut(s) 232, 312, 826, 1086, 1448
Hin6I GCGC 2 cut(s) 141, 168
HinP1I GCGC 2 cut(s) 141, 168
HincII GTYRAC 2 cut(s) 202, 399
HindII GTYRAC 2 cut(s) 202, 399
HpaII CCGG 2 cut(s) 75, 110
HphI GGTGA 3 cut(s) 40, 1106, 1503
Hpy166II GTNNAC 6 cut(s) 202, 364, 399, 613, 1423, 1503
Hpy188I TCNGA 8 cut(s) 33, 40, 57, 619, 844, 928, 1036, 1323
Hpy188III TCNNGA 7 cut(s) 194, 702, 814, 1059, 1175, 1283, 1365
Hpy8I GTNNAC 6 cut(s) 202, 364, 399, 613, 1423, 1503
Hpy99I CGWCG 2 cut(s) 148, 1027
HpyAV CCTTC 7 cut(s) 22, 401, 615, 710, 839, 1093, 1121
HpyCH4III ACNGT 4 cut(s) 263, 836, 967, 1206
HpyCH4IV ACGT 2 cut(s) 300, 1105
HpyCH4V TGCA 9 cut(s) 327, 500, 826, 1049, 1082, 1423, 1541, 1562, 1601
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 140, 1079
HpyF3I CTNAG 2 cut(s) 1482, 1497
HpySE526I ACGT 2 cut(s) 300, 1105
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 5 cut(s) 232, 312, 826, 1086, 1448
HspAI GCGC 2 cut(s) 141, 168
KpnI GGTACC 1 cut(s) 158
Kzo9I GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
LmnI GCTCC 5 cut(s) 77, 89, 125, 482, 1466
Lsp1109I GCAGC 5 cut(s) 71, 115, 547, 1033, 1172
LweI GCATC 3 cut(s) 110, 791, 1009
MaeI CTAG 5 cut(s) 432, 900, 1052, 1118, 1463
MaeII ACGT 2 cut(s) 300, 1105
MaeIII GTNAC 2 cut(s) 695, 1064
MalI GATC 7 cut(s) 307, 812, 1057, 1275, 1300, 1341, 1443
MboI GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
MboII GAAGA 9 cut(s) 96, 224, 245, 294, 326, 464, 521, 861, 1007
MflI RGATCY 2 cut(s) 810, 1055
MhlI GDGCHC 4 cut(s) 94, 461, 1425, 1589
MluCI AATT 8 cut(s) 746, 771, 838, 864, 881, 998, 1221, 1265
MlyI GAGTC 7 cut(s) 76, 209, 524, 556, 608, 692, 1241
MmeI TCCRAC 4 cut(s) 670, 766, 1260, 1269
Mph1103I ATGCAT 1 cut(s) 1084
MseI TTAA 6 cut(s) 476, 1001, 1077, 1155, 1329, 1523
MslI CAYNNNNRTG 3 cut(s) 227, 379, 928
MspCI CTTAAG 1 cut(s) 1154
MspI CCGG 2 cut(s) 75, 110
MspR9I CCNGG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 2 cut(s) 143, 1428
MwoI GCNNNNNNNGC 3 cut(s) 98, 140, 1079
NdeII GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
NlaIII CATG 5 cut(s) 232, 312, 826, 1086, 1448
NlaIV GGNNCC 4 cut(s) 156, 412, 484, 1369
NmuCI GTSAC 2 cut(s) 695, 1064
NsiI ATGCAT 1 cut(s) 1084
NspI RCATGY 1 cut(s) 826
NspV TTCGAA 1 cut(s) 15
PaeI GCATGC 1 cut(s) 826
PctI GAATGC 1 cut(s) 433
PfeI GAWTC 3 cut(s) 62, 190, 1508
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 6 cut(s) 86, 130, 133, 562, 1048, 1162
PleI GAGTC 7 cut(s) 75, 209, 523, 555, 608, 692, 1240
PpsI GAGTC 7 cut(s) 75, 209, 523, 555, 608, 692, 1240
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 1589
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 4 cut(s) 156, 412, 484, 1369
PspPI GGNCC 1 cut(s) 241
PspPPI RGGWCCY 1 cut(s) 241
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PsuI RGATCY 2 cut(s) 810, 1055
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 3 cut(s) 156, 643, 1169
RsaNI GTAC 3 cut(s) 155, 642, 1168
RseI CAYNNNNRTG 3 cut(s) 227, 379, 928
SacI GAGCTC 1 cut(s) 1589
SaqAI TTAA 6 cut(s) 476, 1001, 1077, 1155, 1329, 1523
SatI GCNGC 6 cut(s) 85, 129, 132, 561, 1047, 1161
Sau3AI GATC 7 cut(s) 305, 810, 1055, 1273, 1298, 1339, 1441
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 7 cut(s) 76, 209, 524, 556, 608, 692, 1241
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 4 cut(s) 94, 461, 1425, 1589
SfaNI GCATC 3 cut(s) 110, 791, 1009
SfcI CTRYAG 2 cut(s) 294, 588
SfuI TTCGAA 1 cut(s) 15
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 3 cut(s) 227, 379, 928
SmlI CTYRAG 1 cut(s) 1154
SmoI CTYRAG 1 cut(s) 1154
SphI GCATGC 1 cut(s) 826
Sse9I AATT 8 cut(s) 746, 771, 838, 864, 881, 998, 1221, 1265
SsiI CCGC 7 cut(s) 87, 123, 131, 794, 798, 1407, 1426
SspI AATATT 1 cut(s) 1191
SspMI CTAG 5 cut(s) 432, 900, 1052, 1118, 1463
SstI GAGCTC 1 cut(s) 1589
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 2 cut(s) 117, 899
TaaI ACNGT 4 cut(s) 263, 836, 967, 1206
TaiI ACGT 2 cut(s) 303, 1108
TaqI TCGA 4 cut(s) 15, 65, 466, 1487
TaqII GACCGA 1 cut(s) 208
TasI AATT 8 cut(s) 746, 771, 838, 864, 881, 998, 1221, 1265
TatI WGTACW 1 cut(s) 1167
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 62, 190, 1508
Tru1I TTAA 6 cut(s) 476, 1001, 1077, 1155, 1329, 1523
Tru9I TTAA 6 cut(s) 476, 1001, 1077, 1155, 1329, 1523
TscAI CASTG 5 cut(s) 156, 166, 268, 615, 1596
TseFI GTSAC 2 cut(s) 695, 1064
TseI GCWGC 5 cut(s) 84, 128, 560, 1046, 1160
Tsp45I GTSAC 2 cut(s) 695, 1064
TspDTI ATGAA 5 cut(s) 420, 687, 1008, 1104, 1519
TspRI CASTG 5 cut(s) 156, 166, 268, 615, 1596
Tth111I GACNNNGTC 1 cut(s) 218
Vha464I CTTAAG 1 cut(s) 1154
VneI GTGCAC 1 cut(s) 1421
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 2 cut(s) 746, 881
XceI RCATGY 1 cut(s) 826
XmaJI CCTAGG 1 cut(s) 899
XspI CTAG 5 cut(s) 432, 900, 1052, 1118, 1463
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.