RLG00000020561

Peptidase of plants and bacteria

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0015977
Physical Location & Seq
Reverse (-)
66297215 .. 66298054
840 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020561

Sequence Viewer

Length: 840 bp
ATGGAGGACCAAATTCAATCCCTGTTCCAACCCCTCATTCACAGAAAACCAGTCACAAACCATGAAACAACCTATGATTCCATATCAAACGCCGGAATTCTCATTCGTTTCATTTCCGTCATCTTGGTCGCAATCCTCTCATTCTGGGCAAACCACGAAGCTTCTAAAGGGTTTGACATCACCGTGCTAAACGACGTCCCTCGAGACCTCCCCGCCGGCCACCGCTTCAACCTCTTCTACGTCGCCAACGACAAAGCCACCCGCATCGTCCTCGACGCCAGCGATTTCATCGAACACCTTCTTTACCCTAGCGACGACAACTTCAAGAAGCAAGTAAACCGTGTCGTTGTGAGGCTCGTCAGCGTGAACCTAACAAACGCCGTGGGAGTTTTCGTCGTAGAAGACGACCGTAACTTTGTGGTGAACATAAGCCCTTCTGTTATGGAAGAGGCCAACGTCGACCGTGCATTAGTGTCGGCAATACGACGTGGCATGGCGCGTGTGTGGCTTTGGGATGGCATAGGGTCCGGTGCTCCGCCGGAGCTTGTGAACGGCATGGTGGAGTACCTAAGGATGGCGGCGGAAGGGTTTGGTGGCCTGAAAAGTGGTGCTTCCGGCTGGAAGTTGCCAGAGGGCGATGGCTACATTTGGTGGGAGGACAAGGACCCTGCAGCCGTGGCAAAGTTGTTGCACTATTATGAGGGGTACAGGAAGGGCTTCATTCAACGGCTGAACAGAGCTATGAAGGATGAGTGGCATGATTGGACGGTGGATGATGCATTAGGCATGCCATTACAAAATTTGTGTCGTAAATATAATTTTTCCAGGTCCAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSP

Protein Analysis

280

Amino Acids

31.62

Weight (kDa)

5.82

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSP PF04450 56 - 272 9.7e-34 Peptidase of plants and bacteria
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42900
fragaria_vesca FvH4_6g38280
prunus_persica Prupe.3G173400_v2.0.a1
pyrus_communis pycom17g13210
rosa_chinensis RchiOBHm_Chr2g0151581 RchiOBHm_Chr2g0151591
rosa_laevigata RLG00000020561
rosa_roxburghii Rroxscaffold_2G00096340
rosa_samantha Rh2AG487900 Rh2BG500400 Rh2BG500500 Rh2CG474400 Rh2DG511600 Rh2DG511700
rosa_wichuraiana Rw2G039990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AccI GTMKAC 1 cut(s) 459
AccII CGCG 1 cut(s) 499
AciI CCGC 6 cut(s) 213, 223, 262, 536, 578, 581
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 3 cut(s) 12, 96, 799
AcyI GRCGYC 2 cut(s) 195, 276
AfaI GTAC 2 cut(s) 566, 707
AfiI CCNNNNNNNGG 1 cut(s) 574
AgsI TTSAA 4 cut(s) 17, 229, 325, 725
AjiI CACGTC 1 cut(s) 488
AjnI CCWGG 1 cut(s) 824
AjuI GAANNNNNNNTTGG 2 cut(s) 21, 53
AluBI AGCT 4 cut(s) 161, 544, 740, 834
AluI AGCT 4 cut(s) 161, 544, 740, 834
Alw21I GWGCWC 1 cut(s) 535
Alw26I GTCTC 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 201
AoxI GGCC 3 cut(s) 217, 450, 595
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 3 cut(s) 12, 96, 799
ArsI GACNNNNNNTTYG 4 cut(s) 398, 430, 790, 822
Asp700I GAANNNNTTC 2 cut(s) 297, 716
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 4 cut(s) 7, 525, 664, 828
AsuHPI GGTGA 2 cut(s) 172, 433
AvaI CYCGRG 1 cut(s) 201
AvaII GGWCC 4 cut(s) 7, 525, 664, 828
AxyI CCTNAGG 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 683
BccI CCATC 3 cut(s) 509, 568, 632
BceAI ACGGC 4 cut(s) 365, 568, 659, 743
BcgI CGANNNNNNTGC 4 cut(s) 253, 287, 456, 490
BciT130I CCWGG 1 cut(s) 826
BcoDI GTCTC 1 cut(s) 198
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 669
BisI GCNGC 2 cut(s) 579, 672
BlsI GCNGC 2 cut(s) 580, 673
Bme1390I CCNGG 1 cut(s) 826
Bme18I GGWCC 4 cut(s) 7, 525, 664, 828
BmeT110I CYCGRG 1 cut(s) 201
BmgBI CACGTC 1 cut(s) 488
BmgT120I GGNCC 4 cut(s) 7, 525, 664, 828
BmiI GGNNCC 2 cut(s) 526, 666
BmrFI CCNGG 1 cut(s) 826
BmsI GCATC 2 cut(s) 273, 766
BpiI GAAGAC 1 cut(s) 408
BsaHI GRCGYC 2 cut(s) 195, 276
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 381, 675
BsaWI WCCGGW 1 cut(s) 527
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 50
Bse21I CCTNAGG 1 cut(s) 569
BseBI CCWGG 1 cut(s) 826
BseDI CCNNGG 2 cut(s) 381, 675
BseGI GGATG 4 cut(s) 520, 579, 754, 778
BseLI CCNNNNNNNGG 1 cut(s) 574
BseNI ACTGG 1 cut(s) 50
BseXI GCAGC 1 cut(s) 683
Bsh1236I CGCG 1 cut(s) 499
Bsh1285I CGRYCG 2 cut(s) 409, 463
BshFI GGCC 3 cut(s) 219, 452, 597
BsiEI CGRYCG 2 cut(s) 409, 463
BsiHKAI GWGCWC 1 cut(s) 535
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 5 cut(s) 93, 216, 528, 539, 615
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 1 cut(s) 198
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 3 cut(s) 219, 452, 597
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 535
BspACI CCGC 6 cut(s) 213, 223, 262, 536, 578, 581
BspANI GGCC 3 cut(s) 219, 452, 597
BspFNI CGCG 1 cut(s) 499
BspLI GGNNCC 2 cut(s) 526, 666
BspMAI CTGCAG 1 cut(s) 673
BspTNI GGTCTC 1 cut(s) 198
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 2 cut(s) 381, 675
BssNI GRCGYC 2 cut(s) 195, 276
Bst2UI CCWGG 1 cut(s) 826
Bst4CI ACNGT 5 cut(s) 184, 341, 410, 464, 769
Bst6I CTCTTC 2 cut(s) 239, 441
BstACI GRCGYC 2 cut(s) 195, 276
BstC8I GCNNGC 3 cut(s) 217, 280, 788
BstDEI CTNAG 1 cut(s) 569
BstDSI CCRYGG 2 cut(s) 381, 675
BstF5I GGATG 4 cut(s) 520, 579, 754, 778
BstFNI CGCG 1 cut(s) 499
BstHHI GCGC 1 cut(s) 499
BstMAI GTCTC 1 cut(s) 198
BstMCI CGRYCG 2 cut(s) 409, 463
BstMWI GCNNNNNNNGC 2 cut(s) 505, 677
BstNI CCWGG 1 cut(s) 826
BstNSI RCATGY 1 cut(s) 790
BstSCI CCNGG 1 cut(s) 824
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 1 cut(s) 683
BstV2I GAAGAC 1 cut(s) 408
Bsu36I CCTNAGG 1 cut(s) 569
BsuRI GGCC 3 cut(s) 219, 452, 597
BtgI CCRYGG 2 cut(s) 381, 675
BtgZI GCGATG 1 cut(s) 651
BtrI CACGTC 1 cut(s) 488
BtsCI GGATG 4 cut(s) 520, 579, 754, 778
Cac8I GCNNGC 3 cut(s) 217, 280, 788
CfoI GCGC 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 4 cut(s) 7, 525, 664, 828
CseI GACGC 1 cut(s) 284
Csp6I GTAC 2 cut(s) 565, 706
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviAII CATG 5 cut(s) 62, 493, 556, 758, 787
CviQI GTAC 2 cut(s) 565, 706
DdeI CTNAG 1 cut(s) 569
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 239, 441
EarI CTCTTC 2 cut(s) 239, 441
EciI GGCGGA 2 cut(s) 525, 596
Eco31I GGTCTC 1 cut(s) 198
Eco47I GGWCC 4 cut(s) 7, 525, 664, 828
Eco81I CCTNAGG 1 cut(s) 569
Eco88I CYCGRG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 664
EcoRI GAATTC 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 824
EcoT22I ATGCAT 1 cut(s) 781
FaeI CATG 5 cut(s) 65, 496, 559, 761, 790
FalI AAGNNNNNCTT 2 cut(s) 595, 627
FaqI GGGAC 1 cut(s) 182
FatI CATG 5 cut(s) 61, 492, 555, 757, 786
FauI CCCGC 2 cut(s) 220, 269
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 2 cut(s) 579, 672
FokI GGATG 4 cut(s) 527, 586, 761, 785
Fsp4HI GCNGC 2 cut(s) 579, 672
FspBI CTAG 1 cut(s) 309
GlaI GCGC 1 cut(s) 498
GluI GCNGC 2 cut(s) 579, 672
HaeIII GGCC 3 cut(s) 219, 452, 597
HapII CCGG 5 cut(s) 93, 216, 528, 539, 615
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 499
Hin1I GRCGYC 2 cut(s) 195, 276
Hin1II CATG 5 cut(s) 65, 496, 559, 761, 790
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 1 cut(s) 77
HpaII CCGG 5 cut(s) 93, 216, 528, 539, 615
HphI GGTGA 2 cut(s) 172, 433
Hpy166II GTNNAC 5 cut(s) 337, 367, 424, 460, 550
Hpy188III TCNNGA 2 cut(s) 203, 325
Hpy8I GTNNAC 5 cut(s) 337, 367, 424, 460, 550
Hpy99I CGWCG 7 cut(s) 197, 245, 278, 317, 398, 461, 489
HpyAV CCTTC 5 cut(s) 308, 444, 578, 706, 739
HpyCH4III ACNGT 5 cut(s) 184, 341, 410, 464, 769
HpyCH4IV ACGT 4 cut(s) 195, 240, 456, 487
HpyCH4V TGCA 4 cut(s) 467, 671, 691, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 677
HpyF3I CTNAG 1 cut(s) 569
HpySE526I ACGT 4 cut(s) 195, 240, 456, 487
Hsp92I GRCGYC 2 cut(s) 195, 276
Hsp92II CATG 5 cut(s) 65, 496, 559, 761, 790
HspAI GCGC 1 cut(s) 497
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
LmnI GCTCC 2 cut(s) 538, 541
Lsp1109I GCAGC 1 cut(s) 683
LweI GCATC 2 cut(s) 273, 766
MaeI CTAG 1 cut(s) 309
MaeII ACGT 4 cut(s) 195, 240, 456, 487
MaeIII GTNAC 2 cut(s) 52, 410
MboII GAAGA 3 cut(s) 226, 413, 458
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 4 cut(s) 12, 96, 799, 817
MmeI TCCRAC 1 cut(s) 52
Mph1103I ATGCAT 1 cut(s) 781
MroNI GCCGGC 1 cut(s) 215
MroXI GAANNNNTTC 2 cut(s) 297, 716
MslI CAYNNNNRTG 2 cut(s) 182, 696
MspI CCGG 5 cut(s) 93, 216, 528, 539, 615
MspR9I CCNGG 1 cut(s) 826
MvaI CCWGG 1 cut(s) 826
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 2 cut(s) 505, 677
NaeI GCCGGC 1 cut(s) 217
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 5 cut(s) 65, 496, 559, 761, 790
NlaIV GGNNCC 2 cut(s) 526, 666
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 781
NspI RCATGY 1 cut(s) 790
PaeI GCATGC 1 cut(s) 790
PaeR7I CTCGAG 1 cut(s) 201
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 279, 402
PdiI GCCGGC 1 cut(s) 217
PdmI GAANNNNTTC 2 cut(s) 297, 716
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 580, 673
PpuMI RGGWCCY 1 cut(s) 664
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 824
PspGI CCWGG 1 cut(s) 824
PspN4I GGNNCC 2 cut(s) 526, 666
PspPI GGNCC 4 cut(s) 7, 525, 664, 828
PspPPI RGGWCCY 1 cut(s) 664
PstI CTGCAG 1 cut(s) 673
RsaI GTAC 2 cut(s) 566, 707
RsaNI GTAC 2 cut(s) 565, 706
RseI CAYNNNNRTG 2 cut(s) 182, 696
SalI GTCGAC 1 cut(s) 458
SatI GCNGC 2 cut(s) 579, 672
Sau96I GGNCC 4 cut(s) 7, 525, 664, 828
ScrFI CCNGG 1 cut(s) 826
SduI GDGCHC 1 cut(s) 535
SfaNI GCATC 2 cut(s) 273, 766
SfcI CTRYAG 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 201
SinI GGWCC 4 cut(s) 7, 525, 664, 828
SlaI CTCGAG 1 cut(s) 201
SmiMI CAYNNNNRTG 2 cut(s) 182, 696
SmlI CTYRAG 1 cut(s) 201
SmoI CTYRAG 1 cut(s) 201
SphI GCATGC 1 cut(s) 790
Sse9I AATT 4 cut(s) 12, 96, 799, 817
SsiI CCGC 6 cut(s) 213, 223, 262, 536, 578, 581
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 1 cut(s) 824
TaaI ACNGT 5 cut(s) 184, 341, 410, 464, 769
TaiI ACGT 4 cut(s) 198, 243, 459, 490
TaqI TCGA 4 cut(s) 202, 273, 291, 459
TasI AATT 4 cut(s) 12, 96, 799, 817
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 1 cut(s) 77
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 671
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 5 cut(s) 78, 100, 277, 709, 758
TspGWI ACGGA 1 cut(s) 106
VpaK11BI GGWCC 4 cut(s) 7, 525, 664, 828
XapI RAATTY 3 cut(s) 12, 96, 799
XceI RCATGY 1 cut(s) 790
XhoI CTCGAG 1 cut(s) 201
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 2 cut(s) 297, 716
XspI CTAG 1 cut(s) 309
ZraI GACGTC 1 cut(s) 196
Zsp2I ATGCAT 1 cut(s) 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.