RLG00000020708

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005889
Physical Location & Seq
Forward (+)
67843848 .. 67849116
5269 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020708

Sequence Viewer

Length: 1278 bp
ATGGCCATGGCTGCTTATTCACTGTTGAATCCCTCTTCCCCAACTATGCTTAATCTCAGCTTTCAGAGAAGTGGTTTAGGTTATCTTCACTCTTCCACCACCACTCATCTTCTCAACTCCAAGCTCAAACGCTCTTCTATCAGGTGCCAATCGACTAGTACTGAAGACCCAAAAACAAAAAGAAATTTACTGGATAATGCAAGCAACCTCCTGACAAATCTTTTAAGTGGGGGAAATCTTGGGTCTATGCCTATAGCTGAAGGTGCAGTCACCGATTTGTTTGATCGACCTCTCTTCTTTTCACTATATGATTGGTTCATAGAGCACGGCTCTGTATATAAGCTTGCATTTGGACCGAAAGCATTTGTTGTTGTATCAGATCCTATTGTTGCAAGGCATATTCTTCGAGAAAATGCATTTGGTTATGATAAGGGAGTTCTGGCTGATATTCTAGAACCAATAATGGGAAAAGGACTTATACCTGCTGACATTGAAACTTGGAAGCAGAGGAGAAGAGTTATTGCTCCTGGGTTCCATGCTGTGTACTTGGAAGCTATGGTCAAAATGTTCAGTGATTGTTCAGAAAGAACAGTATTAAAATTTGAGAAGTTACTAGAAAGAGAGGAGCTTAATGGGAGAAAGACAATTGAAGTGGACCTTGAAGCAGAGTTTTTGAGTCTGGGGCTTGATATTATTGGGCTTGGTGTCTTCAACTATGACTTTGGTTCTGTTACTAAAGAATCTCCTGTCATTAAGGCAGTATATGGTACTCTCTCTGAAGCTGAACATAGGTCCACTTTTTACATCCCATATTGGAAATTTCCCCTGGCAAGGTGGATTGTTCCCCGGCAACGAAAGTTTTATGGTGACCTGAAGGTTATAAATGATTGTCTTGATGGACTCATCAGAAATGCAAAAGAGACCAGACAGGAAACAGATGTCGAAAAACTGCAACAAAGGGACTACTCTAATCTGAAGGATGCAAGTCTATTGCGTTTCCTAGTTGATATGCGCGGAGCTGATGTTGATGACCGTCAGCTGAGAGATGACTTGATGACGATGCTTATTGCTGGCCATGAAACAACAGCAGCTGTCCTTACTTGGACTATTTTCTTCCTTGCACAAAACCCGTCCAAGATGAAGAAAGCACAAACAGAAATTGATTCAGTATTTGGACAAAGTCGACCAACTTTAGATTCAATTAAAAAATTGGAGTATGTCCCTAAGACATTTGCTGAACTTTTACCACTCACTTTCCATGCTTTATACATACAATGA

Protein Analysis

426

Amino Acids

48.13

Weight (kDa)

7.1

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 105 - 415 1.1e-40 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 881
Acc36I ACCTGC 1 cut(s) 490
AccB1I GGYRCC 1 cut(s) 144
AccI GTMKAC 1 cut(s) 1183
AccII CGCG 1 cut(s) 1014
AciI CCGC 1 cut(s) 1014
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 2 cut(s) 3, 1072
AcsI RAATTY 3 cut(s) 184, 599, 818
AcuI CTGAAG 5 cut(s) 183, 279, 798, 893, 995
AfaI GTAC 3 cut(s) 160, 545, 769
AfiI CCNNNNNNNGG 2 cut(s) 464, 831
AgsI TTSAA 6 cut(s) 28, 494, 650, 662, 712, 1200
AhlI ACTAGT 1 cut(s) 155
AjnI CCWGG 2 cut(s) 526, 825
AjuI GAANNNNNNNTTGG 4 cut(s) 402, 434, 1180, 1212
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 1 cut(s) 914
AlwI GGATC 1 cut(s) 374
AlwNI CAGNNNCTG 1 cut(s) 1091
AoxI GGCC 2 cut(s) 3, 1072
ApeKI GCWGC 2 cut(s) 11, 1088
ApoI RAATTY 3 cut(s) 184, 599, 818
AspLEI GCGC 1 cut(s) 1014
AspS9I GGNCC 3 cut(s) 353, 655, 792
AsuC2I CCSGG 1 cut(s) 847
AsuHPI GGTGA 2 cut(s) 262, 878
AvaII GGWCC 3 cut(s) 353, 655, 792
BalI TGGCCA 2 cut(s) 5, 1074
BanI GGYRCC 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 171, 700
Bbv12I GWGCWC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 1100
BccI CCATC 1 cut(s) 890
BceAI ACGGC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 2 cut(s) 528, 827
BcnI CCSGG 1 cut(s) 847
BcoDI GTCTC 1 cut(s) 914
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 4 cut(s) 156, 452, 614, 1001
BfmI CTRYAG 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 490
BisI GCNGC 2 cut(s) 12, 1089
BlsI GCNGC 2 cut(s) 13, 1090
BmcAI AGTACT 1 cut(s) 160
Bme1390I CCNGG 3 cut(s) 528, 827, 847
Bme18I GGWCC 3 cut(s) 353, 655, 792
BmgT120I GGNCC 3 cut(s) 353, 655, 792
BmiI GGNNCC 2 cut(s) 146, 533
BmrFI CCNGG 3 cut(s) 528, 827, 847
BmsI GCATC 2 cut(s) 970, 1050
BpiI GAAGAC 2 cut(s) 171, 700
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BpuMI CCSGG 1 cut(s) 847
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 4 cut(s) 6, 527, 825, 845
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 831
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 2 cut(s) 528, 827
BseDI CCNNGG 4 cut(s) 6, 527, 825, 845
BseGI GGATG 2 cut(s) 804, 985
BseLI CCNNNNNNNGG 2 cut(s) 464, 831
BseMII CTCAG 2 cut(s) 70, 1031
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 2 cut(s) 523, 638
BseXI GCAGC 1 cut(s) 1100
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 2 cut(s) 5, 1074
BshNI GGYRCC 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 327
BsiSI CCGG 1 cut(s) 847
BslFI GGGAC 2 cut(s) 974, 1205
BslI CCNNNNNNNGG 2 cut(s) 464, 831
BsmAI GTCTC 1 cut(s) 914
BsmFI GGGAC 2 cut(s) 974, 1205
BsnI GGCC 2 cut(s) 5, 1074
Bso31I GGTCTC 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 2 cut(s) 283, 379
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 1 cut(s) 1014
BspANI GGCC 2 cut(s) 5, 1074
BspCNI CTCAG 2 cut(s) 69, 1032
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 2 cut(s) 146, 533
BspMI ACCTGC 1 cut(s) 490
BspPI GGATC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 914
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 4 cut(s) 6, 527, 825, 845
BssMI GATC 2 cut(s) 283, 379
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 528, 827
Bst4CI ACNGT 3 cut(s) 24, 592, 1034
Bst6I CTCTTC 5 cut(s) 40, 97, 139, 299, 508
BstC8I GCNNGC 3 cut(s) 202, 345, 1072
BstDEI CTNAG 3 cut(s) 56, 1040, 1224
BstDSI CCRYGG 1 cut(s) 6
BstEII GGTNACC 1 cut(s) 866
BstF5I GGATG 2 cut(s) 804, 985
BstFNI CGCG 1 cut(s) 1014
BstHHI GCGC 1 cut(s) 1014
BstKTI GATC 2 cut(s) 286, 382
BstMAI GTCTC 1 cut(s) 914
BstMBI GATC 2 cut(s) 283, 379
BstMWI GCNNNNNNNGC 2 cut(s) 11, 263
BstNI CCWGG 2 cut(s) 528, 827
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 3 cut(s) 526, 825, 845
BstSFI CTRYAG 1 cut(s) 252
BstUI CGCG 1 cut(s) 1014
BstV1I GCAGC 1 cut(s) 1100
BstV2I GAAGAC 2 cut(s) 171, 700
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 2 cut(s) 5, 1074
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 2 cut(s) 804, 985
BtsIMutI CAGTG 2 cut(s) 20, 577
BveI ACCTGC 1 cut(s) 490
Cac8I GCNNGC 3 cut(s) 202, 345, 1072
CaiI CAGNNNCTG 1 cut(s) 1091
CfoI GCGC 1 cut(s) 1014
Cfr13I GGNCC 3 cut(s) 353, 655, 792
Csp6I GTAC 3 cut(s) 159, 544, 768
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviAII CATG 4 cut(s) 7, 536, 1076, 1259
CviQI GTAC 3 cut(s) 159, 544, 768
DdeI CTNAG 3 cut(s) 56, 1040, 1224
DpnI GATC 2 cut(s) 285, 381
DpnII GATC 2 cut(s) 283, 379
EaeI YGGCCR 2 cut(s) 3, 1072
Eam1104I CTCTTC 5 cut(s) 40, 97, 139, 299, 508
EarI CTCTTC 5 cut(s) 40, 97, 139, 299, 508
Eco130I CCWWGG 1 cut(s) 6
Eco31I GGTCTC 1 cut(s) 914
Eco47I GGWCC 3 cut(s) 353, 655, 792
Eco57I CTGAAG 5 cut(s) 183, 279, 798, 893, 995
Eco91I GGTNACC 1 cut(s) 866
EcoO65I GGTNACC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 526, 825
EcoT14I CCWWGG 1 cut(s) 6
EcoT22I ATGCAT 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 4 cut(s) 10, 539, 1079, 1262
FalI AAGNNNNNCTT 2 cut(s) 642, 674
FaqI GGGAC 2 cut(s) 974, 1205
FatI CATG 4 cut(s) 6, 535, 1075, 1258
FblI GTMKAC 1 cut(s) 1183
Fnu4HI GCNGC 2 cut(s) 12, 1089
FokI GGATG 2 cut(s) 791, 992
Fsp4HI GCNGC 2 cut(s) 12, 1089
FspBI CTAG 4 cut(s) 156, 452, 614, 1001
GlaI GCGC 1 cut(s) 1013
GluI GCNGC 2 cut(s) 12, 1089
HaeIII GGCC 2 cut(s) 5, 1074
HapII CCGG 1 cut(s) 847
HhaI GCGC 1 cut(s) 1014
Hin1II CATG 4 cut(s) 10, 539, 1079, 1262
Hin6I GCGC 1 cut(s) 1012
HinP1I GCGC 1 cut(s) 1012
HincII GTYRAC 1 cut(s) 1184
HindII GTYRAC 1 cut(s) 1184
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 6 cut(s) 28, 676, 740, 900, 1163, 1196
HpaII CCGG 1 cut(s) 847
HphI GGTGA 2 cut(s) 262, 878
Hpy166II GTNNAC 4 cut(s) 544, 655, 795, 1184
Hpy188I TCNGA 6 cut(s) 66, 379, 583, 778, 908, 975
Hpy188III TCNNGA 4 cut(s) 211, 407, 452, 893
Hpy8I GTNNAC 4 cut(s) 544, 655, 795, 1184
HpyAV CCTTC 3 cut(s) 254, 868, 970
HpyCH4III ACNGT 3 cut(s) 24, 592, 1034
HpyCH4V TGCA 9 cut(s) 200, 266, 347, 392, 416, 914, 952, 983, 1121
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 263
HpyF3I CTNAG 3 cut(s) 56, 1040, 1224
Hsp92II CATG 4 cut(s) 10, 539, 1079, 1262
HspAI GCGC 1 cut(s) 1012
Kzo9I GATC 2 cut(s) 283, 379
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 3 cut(s) 529, 625, 1016
Lsp1109I GCAGC 1 cut(s) 1100
LweI GCATC 2 cut(s) 970, 1050
MaeI CTAG 4 cut(s) 156, 452, 614, 1001
MaeIII GTNAC 4 cut(s) 268, 609, 730, 866
MalI GATC 2 cut(s) 285, 381
MboI GATC 2 cut(s) 283, 379
MfeI CAATTG 1 cut(s) 645
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 2 cut(s) 5, 1074
MluCI AATT 7 cut(s) 184, 599, 645, 818, 1158, 1200, 1208
MluNI TGGCCA 2 cut(s) 5, 1074
MlyI GAGTC 2 cut(s) 685, 894
MnlI CCTC 5 cut(s) 43, 218, 300, 501, 616
Mox20I TGGCCA 2 cut(s) 5, 1074
Mph1103I ATGCAT 1 cut(s) 418
MscI TGGCCA 2 cut(s) 5, 1074
MseI TTAA 6 cut(s) 51, 224, 596, 630, 753, 1203
Msp20I TGGCCA 2 cut(s) 5, 1074
MspA1I CMGCKG 2 cut(s) 1039, 1091
MspI CCGG 1 cut(s) 847
MspR9I CCNGG 3 cut(s) 528, 827, 847
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 2 cut(s) 528, 827
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 2 cut(s) 11, 263
NciI CCSGG 1 cut(s) 847
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 283, 379
NlaIII CATG 4 cut(s) 10, 539, 1079, 1262
NlaIV GGNNCC 2 cut(s) 146, 533
NmuCI GTSAC 2 cut(s) 268, 866
NsiI ATGCAT 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 139
PfeI GAWTC 4 cut(s) 28, 740, 1163, 1196
PflFI GACNNNGTC 1 cut(s) 1179
PkrI GCNGC 2 cut(s) 13, 1090
PleI GAGTC 2 cut(s) 684, 894
PpsI GAGTC 2 cut(s) 684, 894
PsiI TTATAA 1 cut(s) 881
Psp6I CCWGG 2 cut(s) 526, 825
PspEI GGTNACC 1 cut(s) 866
PspGI CCWGG 2 cut(s) 526, 825
PspN4I GGNNCC 2 cut(s) 146, 533
PspPI GGNCC 3 cut(s) 353, 655, 792
PstNI CAGNNNCTG 1 cut(s) 1091
PsuI RGATCY 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 1179
PvuII CAGCTG 2 cut(s) 1039, 1091
RsaI GTAC 3 cut(s) 160, 545, 769
RsaNI GTAC 3 cut(s) 159, 544, 768
SalI GTCGAC 1 cut(s) 1182
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 6 cut(s) 51, 224, 596, 630, 753, 1203
SatI GCNGC 2 cut(s) 12, 1089
Sau3AI GATC 2 cut(s) 283, 379
Sau96I GGNCC 3 cut(s) 353, 655, 792
ScaI AGTACT 1 cut(s) 160
SchI GAGTC 2 cut(s) 685, 894
ScrFI CCNGG 3 cut(s) 528, 827, 847
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 2 cut(s) 970, 1050
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 3 cut(s) 353, 655, 792
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 7 cut(s) 184, 599, 645, 818, 1158, 1200, 1208
SsiI CCGC 1 cut(s) 1014
SspMI CTAG 4 cut(s) 156, 452, 614, 1001
StyD4I CCNGG 3 cut(s) 526, 825, 845
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 3 cut(s) 24, 592, 1034
TaqI TCGA 5 cut(s) 152, 286, 406, 942, 1183
TaqII GACCGA 1 cut(s) 370
TasI AATT 7 cut(s) 184, 599, 645, 818, 1158, 1200, 1208
TatI WGTACW 2 cut(s) 158, 543
TfiI GAWTC 4 cut(s) 28, 740, 1163, 1196
Tru1I TTAA 6 cut(s) 51, 224, 596, 630, 753, 1203
Tru9I TTAA 6 cut(s) 51, 224, 596, 630, 753, 1203
TscAI CASTG 2 cut(s) 27, 577
TseFI GTSAC 2 cut(s) 268, 866
TseI GCWGC 2 cut(s) 11, 1088
Tsp45I GTSAC 2 cut(s) 268, 866
TspDTI ATGAA 3 cut(s) 307, 1092, 1154
TspRI CASTG 2 cut(s) 27, 577
Tth111I GACNNNGTC 1 cut(s) 1179
VpaK11BI GGWCC 3 cut(s) 353, 655, 792
XapI RAATTY 3 cut(s) 184, 599, 818
XbaI TCTAGA 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 1183
XspI CTAG 4 cut(s) 156, 452, 614, 1001
ZrmI AGTACT 1 cut(s) 160
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.