Rw2G041300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005889
Physical Location & Seq
Forward (+)
67358279 .. 67362861
4583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G041300.1

Sequence Viewer

Length: 1734 bp
ATGGCTGCTTATTCACTGTTGAATCCCTCTTCCCCAACTCTGCTTAATCTCAGCTTTCAGAGAAGTGATTTTGGTTATCTTCACTCTTCCTCCACCACTCATCTTCTCAACTCCAAGCTCAAACGCTCTTCTATCAGGTGCCAATCGACTAGTACTGAAGACCCAAAAACAAAAAGAAATTTACTGGATAATGCAAGCAACCTCCTGACAAATCTTTTAAGTGGGGGAAATCTTGGGTCTATGCCCATAGCTGAAGGTGCAGTCACCGATTTGTTTGATCGGCCTCTCTTCTTTTCACTATATGATTGGTTCATAGAGCACGGCTCTGTGTATAAGCTTGCATTTGGACCAAAAGCATTTGTTGTTGTATCAGATCCTATTGTTGCAAGGCATATTCTTCGAGAAAATGCATTTGGTTATGATAAGGGAGTTCTGGCTGATATTCTAGAACCAATAATGGGAAAAGGACTTATACCTGCTGACCTTGAAACTTGGAAGCAGAGGAGAAGAGTTATTGCTCCTGGGTTCCATGCTGTATACTTGGAAGCTATGGTCAACATGTTCAGTGATTGTTCGGAAAGAACAGTATTAAAATTTGAGAAGTTACTAGAAAGAGAGGAGCTGAATGGGAGAAAGACAATTGAAGTGGACCTTGAAGCAGAGTTTTTGAGTCTGGGGCTTGATATTATTGGGCTTGGTGTCTTCAACTATGACTTTGGTTCTGTTACTAAAGAATCTCCTGTCATTAAGGCAGTATATGGTACTCTCTCTGAAGCTGAACATAGGTCCACTTTTTACATCCCATATTGGAAATTTCCCCTGGCAAGGTGGATTGTTCCCCGGCAACGGAAGTTTTATGGTGACCTGAAGGTTATAAATGATTGTCTTGATGGACTCATCAGAAATGCAAAAGAGACCAGACAGGAAACAGATGTCGAAAAACTGCAACAAAGGGACTACTCTAATCTGAAGGATGCAAGTCTATTGCGTTTCCTAGTTGATATGCGTGGAGCTGATGTTGATGACCGTCAGCTGAGAGATGACTTGATGACGATGCTTATTGCCGGCCATGAAACAACAGCAGCTGTCCTTACCTGGGCTATTTTCTTCCTTGCACAAAACCCGTCCAAGATGAAGAAAGCACAAACAGAAATTGATTCAGTACTTGGACAAAGTCGACCAACTTTAGATTCAATTAAAAAATTGGAGTACACAAGATTTATTGTTGCGGAGTCTCTTCGTTTGTATCCTCAACCCCCCTTGCTTATTAGACGTTCTCTAAAACCAGATACACTACCAGGAGGGTACAAGGGTGAAACAGGTGGTTATAAGATTCCCGCTGGAACCGATATCTTTATCTCTGTATACAATCTCCATAGATCTCCATATTACTGGGACCGTCCTAATGTATTCGAACCAGAGAGGTTTTCAGTGCAAAAGAATAGTGACATAGAAGGATGGGCTGGCTTTGACCCTTCTCGAAGCCCTGGAGCATATTATCCAAATGAGATTATGGCAGATTTCTCCTTCTTACCTTTCGGTGGAGGGCCAAGAAAATGCGTCGGGGACCAATTTGCTCTGTTGGAGTCGACTGTATCTTTGGCAATGCTGTTGCAGAAGTTCAATGTAGAGCTAAGAGGATCTCCGGACTCGGTTGGACTAGTAACAGGGGCAACCATCCATACCAAAAATGGAATGTGGTGCAAATTAAGTAAGAGGTCTGATGTACAGTGA

Protein Analysis

577

Amino Acids

65.02

Weight (kDa)

7.62

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 103 - 549 2e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 875, 1329
Acc36I ACCTGC 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 138
AccI GTMKAC 4 cut(s) 537, 1177, 1365, 1589
AccIII TCCGGA 1 cut(s) 1645
AciI CCGC 2 cut(s) 1229, 1338
AclWI GGATC 2 cut(s) 368, 1648
AcoI YGGCCR 1 cut(s) 1066
AcsI RAATTY 3 cut(s) 178, 593, 812
AcuI CTGAAG 5 cut(s) 177, 273, 792, 887, 989
AfaI GTAC 6 cut(s) 154, 763, 1164, 1211, 1307, 1728
AfiI CCNNNNNNNGG 5 cut(s) 458, 825, 846, 1096, 1541
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 7 cut(s) 22, 488, 644, 656, 706, 1194, 1624
AhlI ACTAGT 2 cut(s) 149, 1660
AjnI CCWGG 5 cut(s) 520, 819, 1094, 1297, 1486
AjuI GAANNNNNNNTTGG 4 cut(s) 396, 428, 1174, 1206
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 908, 1239
AlwI GGATC 2 cut(s) 368, 1648
AlwNI CAGNNNCTG 1 cut(s) 1085
Aor13HI TCCGGA 1 cut(s) 1645
AoxI GGCC 3 cut(s) 281, 1066, 1547
ApeKI GCWGC 2 cut(s) 5, 1082
ApoI RAATTY 3 cut(s) 178, 593, 812
ArsI GACNNNNNNTTYG 2 cut(s) 1582, 1614
AspS9I GGNCC 6 cut(s) 347, 649, 786, 1396, 1547, 1567
AsuC2I CCSGG 1 cut(s) 841
AsuHPI GGTGA 3 cut(s) 256, 872, 1325
AsuII TTCGAA 1 cut(s) 1413
AvaII GGWCC 5 cut(s) 347, 649, 786, 1396, 1567
BaeI ACNNNNGTAYC 2 cut(s) 1297, 1330
BanI GGYRCC 1 cut(s) 138
BbsI GAAGAC 2 cut(s) 165, 694
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 1094
BccI CCATC 3 cut(s) 884, 1452, 1685
BceAI ACGGC 1 cut(s) 337
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 5 cut(s) 522, 821, 1096, 1299, 1488
BciVI GTATCC 1 cut(s) 1257
BcnI CCSGG 1 cut(s) 841
BcoDI GTCTC 2 cut(s) 908, 1239
BcuI ACTAGT 2 cut(s) 149, 1660
BfaI CTAG 5 cut(s) 150, 446, 608, 995, 1661
BfuAI ACCTGC 1 cut(s) 484
BfuI GTATCC 1 cut(s) 1257
BglII AGATCT 1 cut(s) 1379
BisI GCNGC 2 cut(s) 6, 1083
BlsI GCNGC 2 cut(s) 7, 1084
BmcAI AGTACT 2 cut(s) 154, 1164
Bme1390I CCNGG 6 cut(s) 522, 821, 841, 1096, 1299, 1488
Bme18I GGWCC 5 cut(s) 347, 649, 786, 1396, 1567
BmgT120I GGNCC 6 cut(s) 347, 649, 786, 1396, 1547, 1567
BmiI GGNNCC 5 cut(s) 140, 527, 1345, 1397, 1568
BmrFI CCNGG 6 cut(s) 522, 821, 841, 1096, 1299, 1488
BmrI ACTGGG 1 cut(s) 1402
BmsI GCATC 2 cut(s) 964, 1044
BmuI ACTGGG 1 cut(s) 1402
BpiI GAAGAC 2 cut(s) 165, 694
BplI GAGNNNNNCTC 2 cut(s) 308, 340
BpmI CTGGAG 1 cut(s) 1509
Bpu14I TTCGAA 1 cut(s) 1413
BpuMI CCSGG 1 cut(s) 841
BsaI GGTCTC 1 cut(s) 908
BsaJI CCNNGG 5 cut(s) 521, 819, 839, 1095, 1486
BsaWI WCCGGW 1 cut(s) 1645
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 1002, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 458, 825, 846, 1096, 1541
Bse118I RCCGGY 1 cut(s) 1064
Bse1I ACTGG 2 cut(s) 189, 1397
Bse3DI GCAATG 1 cut(s) 1611
BseAI TCCGGA 1 cut(s) 1645
BseBI CCWGG 5 cut(s) 522, 821, 1096, 1299, 1488
BseDI CCNNGG 5 cut(s) 521, 819, 839, 1095, 1486
BseGI GGATG 4 cut(s) 798, 979, 1463, 1677
BseLI CCNNNNNNNGG 5 cut(s) 458, 825, 846, 1096, 1541
BseMI GCAATG 1 cut(s) 1611
BseMII CTCAG 2 cut(s) 64, 1025
BseNI ACTGG 2 cut(s) 189, 1397
BseRI GAGGAG 2 cut(s) 517, 632
BseXI GCAGC 1 cut(s) 1094
BsgI GTGCAG 1 cut(s) 279
BshFI GGCC 3 cut(s) 283, 1068, 1549
BshNI GGYRCC 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 3 cut(s) 841, 1065, 1646
BslFI GGGAC 3 cut(s) 968, 1409, 1580
BslI CCNNNNNNNGG 5 cut(s) 458, 825, 846, 1096, 1541
BsmAI GTCTC 2 cut(s) 908, 1239
BsmFI GGGAC 3 cut(s) 968, 1409, 1580
BsnI GGCC 3 cut(s) 283, 1068, 1549
Bso31I GGTCTC 1 cut(s) 908
Bsp119I TTCGAA 1 cut(s) 1413
Bsp1286I GDGCHC 1 cut(s) 321
Bsp13I TCCGGA 1 cut(s) 1645
Bsp1407I TGTACA 1 cut(s) 1726
Bsp143I GATC 4 cut(s) 277, 373, 1379, 1640
BspACI CCGC 2 cut(s) 1229, 1338
BspANI GGCC 3 cut(s) 283, 1068, 1549
BspCNI CTCAG 2 cut(s) 63, 1026
BspEI TCCGGA 1 cut(s) 1645
BspLI GGNNCC 5 cut(s) 140, 527, 1345, 1397, 1568
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 2 cut(s) 368, 1648
BspQI GCTCTTC 1 cut(s) 133
BspT104I TTCGAA 1 cut(s) 1413
BspT107I GGYRCC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 908
BsrDI GCAATG 1 cut(s) 1611
BsrFI RCCGGY 1 cut(s) 1064
BsrGI TGTACA 1 cut(s) 1726
BsrI ACTGG 2 cut(s) 189, 1397
BssAI RCCGGY 1 cut(s) 1064
BssECI CCNNGG 5 cut(s) 521, 819, 839, 1095, 1486
BssMI GATC 4 cut(s) 277, 373, 1379, 1640
BssNAI GTATAC 2 cut(s) 538, 1366
Bst1107I GTATAC 2 cut(s) 538, 1366
Bst2UI CCWGG 5 cut(s) 522, 821, 1096, 1299, 1488
Bst4CI ACNGT 6 cut(s) 18, 586, 1028, 1400, 1594, 1731
Bst6I CTCTTC 6 cut(s) 34, 91, 133, 293, 502, 1242
BstAUI TGTACA 1 cut(s) 1726
BstBI TTCGAA 1 cut(s) 1413
BstC8I GCNNGC 4 cut(s) 196, 339, 1066, 1465
BstDEI CTNAG 3 cut(s) 50, 1034, 1634
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 4 cut(s) 798, 979, 1463, 1677
BstKTI GATC 4 cut(s) 280, 376, 1382, 1643
BstMAI GTCTC 2 cut(s) 908, 1239
BstMBI GATC 4 cut(s) 277, 373, 1379, 1640
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNI CCWGG 5 cut(s) 522, 821, 1096, 1299, 1488
BstNSI RCATGY 1 cut(s) 562
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 6 cut(s) 520, 819, 839, 1094, 1297, 1486
BstV1I GCAGC 1 cut(s) 1094
BstV2I GAAGAC 2 cut(s) 165, 694
BstX2I RGATCY 3 cut(s) 373, 1379, 1640
BstXI CCANNNNNNTGG 1 cut(s) 1392
BstYI RGATCY 3 cut(s) 373, 1379, 1640
BstZ17I GTATAC 2 cut(s) 538, 1366
BsuI GTATCC 1 cut(s) 1257
BsuRI GGCC 3 cut(s) 283, 1068, 1549
BtsCI GGATG 4 cut(s) 798, 979, 1463, 1677
BtsIMutI CAGTG 3 cut(s) 14, 571, 1437
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 4 cut(s) 196, 339, 1066, 1465
CaiI CAGNNNCTG 1 cut(s) 1085
Cfr10I RCCGGY 1 cut(s) 1064
Cfr13I GGNCC 6 cut(s) 347, 649, 786, 1396, 1547, 1567
CseI GACGC 1 cut(s) 1549
Csp6I GTAC 6 cut(s) 153, 762, 1163, 1210, 1306, 1727
CspCI CAANNNNNGTGG 2 cut(s) 627, 662
CviAII CATG 3 cut(s) 530, 559, 1070
CviQI GTAC 6 cut(s) 153, 762, 1163, 1210, 1306, 1727
DdeI CTNAG 3 cut(s) 50, 1034, 1634
DpnI GATC 4 cut(s) 279, 375, 1381, 1642
DpnII GATC 4 cut(s) 277, 373, 1379, 1640
EaeI YGGCCR 1 cut(s) 1066
Eam1104I CTCTTC 6 cut(s) 34, 91, 133, 293, 502, 1242
EarI CTCTTC 6 cut(s) 34, 91, 133, 293, 502, 1242
Eco31I GGTCTC 1 cut(s) 908
Eco32I GATATC 1 cut(s) 1351
Eco47I GGWCC 5 cut(s) 347, 649, 786, 1396, 1567
Eco57I CTGAAG 5 cut(s) 177, 273, 792, 887, 989
Eco91I GGTNACC 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 5 cut(s) 520, 819, 1094, 1297, 1486
EcoRV GATATC 1 cut(s) 1351
EcoT22I ATGCAT 1 cut(s) 412
FaeI CATG 3 cut(s) 533, 562, 1073
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 3 cut(s) 968, 1409, 1580
FatI CATG 3 cut(s) 529, 558, 1069
FauI CCCGC 1 cut(s) 1345
FblI GTMKAC 4 cut(s) 537, 1177, 1365, 1589
Fnu4HI GCNGC 2 cut(s) 6, 1083
FokI GGATG 4 cut(s) 785, 986, 1470, 1664
Fsp4HI GCNGC 2 cut(s) 6, 1083
FspBI CTAG 5 cut(s) 150, 446, 608, 995, 1661
GluI GCNGC 2 cut(s) 6, 1083
GsuI CTGGAG 1 cut(s) 1509
HaeIII GGCC 3 cut(s) 283, 1068, 1549
HapII CCGG 3 cut(s) 841, 1065, 1646
HgaI GACGC 1 cut(s) 1549
Hin1II CATG 3 cut(s) 533, 562, 1073
HincII GTYRAC 3 cut(s) 556, 1178, 1590
HindII GTYRAC 3 cut(s) 556, 1178, 1590
HindIII AAGCTT 1 cut(s) 335
HpaII CCGG 3 cut(s) 841, 1065, 1646
HphI GGTGA 3 cut(s) 256, 872, 1325
Hpy166II GTNNAC 8 cut(s) 538, 556, 649, 789, 1178, 1212, 1366, 1590
Hpy188I TCNGA 7 cut(s) 60, 373, 577, 772, 902, 969, 1723
Hpy188III TCNNGA 6 cut(s) 205, 401, 446, 887, 1479, 1646
Hpy8I GTNNAC 8 cut(s) 538, 556, 649, 789, 1178, 1212, 1366, 1590
Hpy99I CGWCG 1 cut(s) 1565
HpyAV CCTTC 6 cut(s) 248, 862, 964, 1448, 1485, 1537
HpyCH4III ACNGT 6 cut(s) 18, 586, 1028, 1400, 1594, 1731
HpyCH4IV ACGT 1 cut(s) 1273
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 3 cut(s) 50, 1034, 1634
HpySE526I ACGT 1 cut(s) 1273
Hsp92II CATG 3 cut(s) 533, 562, 1073
Kpn2I TCCGGA 1 cut(s) 1645
KroI GCCGGC 1 cut(s) 1064
KroNI GCCGGC 1 cut(s) 1066
Kzo9I GATC 4 cut(s) 277, 373, 1379, 1640
LguI GCTCTTC 1 cut(s) 133
LmnI GCTCC 4 cut(s) 523, 619, 1010, 1490
Lsp1109I GCAGC 1 cut(s) 1094
LweI GCATC 2 cut(s) 964, 1044
MaeI CTAG 5 cut(s) 150, 446, 608, 995, 1661
MaeII ACGT 1 cut(s) 1273
MaeIII GTNAC 6 cut(s) 262, 603, 724, 860, 1445, 1663
MalI GATC 4 cut(s) 279, 375, 1381, 1642
MboI GATC 4 cut(s) 277, 373, 1379, 1640
MfeI CAATTG 1 cut(s) 639
MflI RGATCY 3 cut(s) 373, 1379, 1640
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 9 cut(s) 178, 593, 639, 812, 1152, 1194, 1202, 1571, 1706
MlyI GAGTC 5 cut(s) 679, 888, 1241, 1595, 1643
MmeI TCCRAC 2 cut(s) 1563, 1636
Mph1103I ATGCAT 1 cut(s) 412
MroI TCCGGA 1 cut(s) 1645
MroNI GCCGGC 1 cut(s) 1064
MseI TTAA 6 cut(s) 45, 218, 590, 747, 1197, 1709
MspA1I CMGCKG 3 cut(s) 1033, 1085, 1340
MspI CCGG 3 cut(s) 841, 1065, 1646
MspR9I CCNGG 6 cut(s) 522, 821, 841, 1096, 1299, 1488
MunI CAATTG 1 cut(s) 639
MvaI CCWGG 5 cut(s) 522, 821, 1096, 1299, 1488
MwoI GCNNNNNNNGC 1 cut(s) 257
NaeI GCCGGC 1 cut(s) 1066
NciI CCSGG 1 cut(s) 841
NdeII GATC 4 cut(s) 277, 373, 1379, 1640
NgoMIV GCCGGC 1 cut(s) 1064
NlaIII CATG 3 cut(s) 533, 562, 1073
NlaIV GGNNCC 5 cut(s) 140, 527, 1345, 1397, 1568
NmuCI GTSAC 3 cut(s) 262, 860, 1445
NsiI ATGCAT 1 cut(s) 412
NspI RCATGY 1 cut(s) 562
NspV TTCGAA 1 cut(s) 1413
PciI ACATGT 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 133
PdiI GCCGGC 1 cut(s) 1066
PfeI GAWTC 5 cut(s) 22, 734, 1157, 1190, 1333
PflFI GACNNNGTC 1 cut(s) 1173
PkrI GCNGC 2 cut(s) 7, 1084
PleI GAGTC 5 cut(s) 678, 888, 1240, 1594, 1643
PpsI GAGTC 5 cut(s) 678, 888, 1240, 1594, 1643
PscI ACATGT 1 cut(s) 558
PsiI TTATAA 2 cut(s) 875, 1329
Psp6I CCWGG 5 cut(s) 520, 819, 1094, 1297, 1486
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 5 cut(s) 520, 819, 1094, 1297, 1486
PspN4I GGNNCC 5 cut(s) 140, 527, 1345, 1397, 1568
PspPI GGNCC 6 cut(s) 347, 649, 786, 1396, 1547, 1567
PstNI CAGNNNCTG 1 cut(s) 1085
PsuI RGATCY 3 cut(s) 373, 1379, 1640
PsyI GACNNNGTC 1 cut(s) 1173
PvuII CAGCTG 2 cut(s) 1033, 1085
RsaI GTAC 6 cut(s) 154, 763, 1164, 1211, 1307, 1728
RsaNI GTAC 6 cut(s) 153, 762, 1163, 1210, 1306, 1727
SalI GTCGAC 2 cut(s) 1176, 1588
SapI GCTCTTC 1 cut(s) 133
SaqAI TTAA 6 cut(s) 45, 218, 590, 747, 1197, 1709
SatI GCNGC 2 cut(s) 6, 1083
Sau3AI GATC 4 cut(s) 277, 373, 1379, 1640
Sau96I GGNCC 6 cut(s) 347, 649, 786, 1396, 1547, 1567
ScaI AGTACT 2 cut(s) 154, 1164
SchI GAGTC 5 cut(s) 679, 888, 1241, 1595, 1643
ScrFI CCNGG 6 cut(s) 522, 821, 841, 1096, 1299, 1488
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 2 cut(s) 964, 1044
SfuI TTCGAA 1 cut(s) 1413
SinI GGWCC 5 cut(s) 347, 649, 786, 1396, 1567
SpeI ACTAGT 2 cut(s) 149, 1660
Sse9I AATT 9 cut(s) 178, 593, 639, 812, 1152, 1194, 1202, 1571, 1706
SsiI CCGC 2 cut(s) 1229, 1338
SspMI CTAG 5 cut(s) 150, 446, 608, 995, 1661
StyD4I CCNGG 6 cut(s) 520, 819, 839, 1094, 1297, 1486
TaaI ACNGT 6 cut(s) 18, 586, 1028, 1400, 1594, 1731
TaiI ACGT 1 cut(s) 1276
TaqI TCGA 7 cut(s) 146, 400, 936, 1177, 1413, 1480, 1589
TasI AATT 9 cut(s) 178, 593, 639, 812, 1152, 1194, 1202, 1571, 1706
TatI WGTACW 4 cut(s) 152, 1162, 1209, 1726
TfiI GAWTC 5 cut(s) 22, 734, 1157, 1190, 1333
Tru1I TTAA 6 cut(s) 45, 218, 590, 747, 1197, 1709
Tru9I TTAA 6 cut(s) 45, 218, 590, 747, 1197, 1709
TscAI CASTG 3 cut(s) 21, 571, 1437
TseFI GTSAC 3 cut(s) 262, 860, 1445
TseI GCWGC 2 cut(s) 5, 1082
Tsp45I GTSAC 3 cut(s) 262, 860, 1445
TspDTI ATGAA 3 cut(s) 301, 1086, 1148
TspGWI ACGGA 1 cut(s) 862
TspRI CASTG 3 cut(s) 21, 571, 1437
Tth111I GACNNNGTC 1 cut(s) 1173
VpaK11BI GGWCC 5 cut(s) 347, 649, 786, 1396, 1567
XapI RAATTY 3 cut(s) 178, 593, 812
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 1 cut(s) 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 1688
XmiI GTMKAC 4 cut(s) 537, 1177, 1365, 1589
XspI CTAG 5 cut(s) 150, 446, 608, 995, 1661
ZrmI AGTACT 2 cut(s) 154, 1164
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.