Rh2CG488900

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0005889
Physical Location & Seq
Forward (+)
65555186 .. 65560742
5557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG488900.1

Sequence Viewer

Length: 1239 bp
ATGTATGATTTTGCAGTTATTGCTCCTGGGTTCCATGCTTTATACTTGGAAGCTATGGTCAACATGTTCAGTGATTGTTCGGAAAGAACAGTATTAAAATTTGAGAAGTTACTAGAAAGCGAGGAGCTGAATGGGAGAAAGACAATTGAAGTGGACCTTGAAGCAGAGTTTTTGAGTCTGGGGCTTGATATTATTGGGCTTGGTGTCTTCAACTATGACTTTGGTTCTGTTACTAAAGAATCTCCTGTCATTAAGGCAGTATATGGTACTCTCTCTGAAGCTGAACATAGGTCCACTTTTTACATCCCATATTGGAAATTTCCCCTGGCAAGGTGGATTGTTCCCCGGCAACGGAAGTTTTATGGTGACCTGAAGGTTATAAATGATTGTCTTGATGGACTCATCAGAAATGCACAAGAGACCAGACAGGAAACAGATGTCGAAAAACTGCAACAAAGGGACTACTCTAATCTGAAGGATGCAAGTCTATTGCGTTTCCTAGTTGATATGCGCGGAGCTGATGTTGATGACCGTCAGCTGAGAGATGACTTGATGACGATGCTTATTGCTGGCCATGAAACAACAGCAGCTGTCCTTACCTGGGCTATTTTCTTCCTTGCACAAAACCCGTCCAAGATGAAGAAAGCACAAACAGAAATTGATTCAGTACTTGGACAAAGTCGACCAACTTTAGATTCAATTAAAAAATTGGAGTACACAAGATTTATTGTTGCAGAGTCTCTTCGTTTGTATCCTCAACCCCCCTTGCTTATTAGACGTTCTCTAAAACCAGATACACTACCAGGAGGGTACAAGGGTGAAAAAGGTGGTTATAAGATTCCCGCTGGAACCGATATCTTTATCTCTGTATACAATCTCCATAGATCTCCATATTACTGGGACCGTCCTAATGTATTCGAACCAGAGAGGTTTTCAGTGCAAAAGAATAGTGACATAGAAGGATGGGCTGGCTTTGACCCTTCTCGAAGCCCTGGAGCATATTATCCAAATGAGATTATGGCAGATTTCTCCTTCTTACCTTTCGGTGGAGGGCCAAGAAAATGCGTCGGGGACCAATTTGCTTTGTTGGAGTCGACTGTATCTTTGGCAATGCTGTTGCAGAAGTTCAATGTAGAGCTAAGAGGATCTCCGGACTCGGTTGGACTAGTAACAGGGGCAACCATCCATACCAAAAATGGAATGTGGTGCAAATTAAGTAAGAGGTCTGATGTACAGTGA

Protein Analysis

412

Amino Acids

46.73

Weight (kDa)

5.89

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 11 - 384 2.4e-65 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 380, 834
AccI GTMKAC 3 cut(s) 682, 870, 1094
AccII CGCG 1 cut(s) 513
AccIII TCCGGA 1 cut(s) 1150
AciI CCGC 2 cut(s) 513, 843
AclWI GGATC 1 cut(s) 1153
AcoI YGGCCR 1 cut(s) 571
AcsI RAATTY 2 cut(s) 98, 317
AcuI CTGAAG 3 cut(s) 297, 392, 494
AfaI GTAC 5 cut(s) 268, 669, 716, 812, 1233
AfiI CCNNNNNNNGG 4 cut(s) 330, 351, 601, 1046
AflIII ACRYGT 1 cut(s) 63
AgsI TTSAA 5 cut(s) 149, 161, 211, 699, 1129
AhlI ACTAGT 1 cut(s) 1165
AjnI CCWGG 5 cut(s) 25, 324, 599, 802, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
AluBI AGCT 7 cut(s) 53, 127, 281, 518, 538, 590, 1138
AluI AGCT 7 cut(s) 53, 127, 281, 518, 538, 590, 1138
Alw26I GTCTC 2 cut(s) 413, 744
AlwI GGATC 1 cut(s) 1153
AlwNI CAGNNNCTG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 1150
AoxI GGCC 2 cut(s) 571, 1052
ApeKI GCWGC 1 cut(s) 587
ApoI RAATTY 2 cut(s) 98, 317
ArsI GACNNNNNNTTYG 2 cut(s) 1087, 1119
AspLEI GCGC 1 cut(s) 513
AspS9I GGNCC 5 cut(s) 154, 291, 901, 1052, 1072
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 2 cut(s) 377, 830
AsuII TTCGAA 1 cut(s) 918
AvaII GGWCC 4 cut(s) 154, 291, 901, 1072
BaeI ACNNNNGTAYC 2 cut(s) 802, 835
BalI TGGCCA 1 cut(s) 573
BbsI GAAGAC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 599
BccI CCATC 3 cut(s) 389, 957, 1190
BciT130I CCWGG 5 cut(s) 27, 326, 601, 804, 993
BciVI GTATCC 1 cut(s) 762
BcnI CCSGG 1 cut(s) 346
BcoDI GTCTC 2 cut(s) 413, 744
BcuI ACTAGT 1 cut(s) 1165
BfaI CTAG 3 cut(s) 113, 500, 1166
BfuI GTATCC 1 cut(s) 762
BglII AGATCT 1 cut(s) 884
BisI GCNGC 1 cut(s) 588
BlsI GCNGC 1 cut(s) 589
BmcAI AGTACT 1 cut(s) 669
Bme1390I CCNGG 6 cut(s) 27, 326, 346, 601, 804, 993
Bme18I GGWCC 4 cut(s) 154, 291, 901, 1072
BmgT120I GGNCC 5 cut(s) 154, 291, 901, 1052, 1072
BmiI GGNNCC 4 cut(s) 32, 850, 902, 1073
BmrFI CCNGG 6 cut(s) 27, 326, 346, 601, 804, 993
BmrI ACTGGG 1 cut(s) 907
BmsI GCATC 2 cut(s) 469, 549
BmuI ACTGGG 1 cut(s) 907
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 918
BpuMI CCSGG 1 cut(s) 346
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 5 cut(s) 26, 324, 344, 600, 991
BsaWI WCCGGW 1 cut(s) 1150
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 4 cut(s) 330, 351, 601, 1046
Bse1I ACTGG 1 cut(s) 902
Bse3DI GCAATG 1 cut(s) 1116
BseAI TCCGGA 1 cut(s) 1150
BseBI CCWGG 5 cut(s) 27, 326, 601, 804, 993
BseDI CCNNGG 5 cut(s) 26, 324, 344, 600, 991
BseGI GGATG 4 cut(s) 303, 484, 968, 1182
BseLI CCNNNNNNNGG 4 cut(s) 330, 351, 601, 1046
BseMI GCAATG 1 cut(s) 1116
BseMII CTCAG 1 cut(s) 530
BseNI ACTGG 1 cut(s) 902
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 1 cut(s) 599
Bsh1236I CGCG 1 cut(s) 513
BshFI GGCC 2 cut(s) 573, 1054
BsiSI CCGG 2 cut(s) 346, 1151
BslFI GGGAC 3 cut(s) 473, 914, 1085
BslI CCNNNNNNNGG 4 cut(s) 330, 351, 601, 1046
BsmAI GTCTC 2 cut(s) 413, 744
BsmFI GGGAC 3 cut(s) 473, 914, 1085
BsnI GGCC 2 cut(s) 573, 1054
Bso31I GGTCTC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 918
Bsp13I TCCGGA 1 cut(s) 1150
Bsp1407I TGTACA 1 cut(s) 1231
Bsp143I GATC 2 cut(s) 884, 1145
BspACI CCGC 2 cut(s) 513, 843
BspANI GGCC 2 cut(s) 573, 1054
BspCNI CTCAG 1 cut(s) 531
BspEI TCCGGA 1 cut(s) 1150
BspFNI CGCG 1 cut(s) 513
BspLI GGNNCC 4 cut(s) 32, 850, 902, 1073
BspPI GGATC 1 cut(s) 1153
BspT104I TTCGAA 1 cut(s) 918
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 1 cut(s) 1116
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 1 cut(s) 902
BssECI CCNNGG 5 cut(s) 26, 324, 344, 600, 991
BssMI GATC 2 cut(s) 884, 1145
BssNAI GTATAC 1 cut(s) 871
Bst1107I GTATAC 1 cut(s) 871
Bst2UI CCWGG 5 cut(s) 27, 326, 601, 804, 993
Bst4CI ACNGT 5 cut(s) 91, 533, 905, 1099, 1236
Bst6I CTCTTC 1 cut(s) 747
BstAPI GCANNNNNTGC 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 1231
BstBI TTCGAA 1 cut(s) 918
BstC8I GCNNGC 2 cut(s) 571, 970
BstDEI CTNAG 2 cut(s) 539, 1139
BstEII GGTNACC 1 cut(s) 365
BstF5I GGATG 4 cut(s) 303, 484, 968, 1182
BstFNI CGCG 1 cut(s) 513
BstHHI GCGC 1 cut(s) 513
BstKTI GATC 2 cut(s) 887, 1148
BstMAI GTCTC 2 cut(s) 413, 744
BstMBI GATC 2 cut(s) 884, 1145
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 5 cut(s) 27, 326, 601, 804, 993
BstNSI RCATGY 1 cut(s) 67
BstPI GGTNACC 1 cut(s) 365
BstSCI CCNGG 6 cut(s) 25, 324, 344, 599, 802, 991
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 1 cut(s) 199
BstX2I RGATCY 2 cut(s) 884, 1145
BstXI CCANNNNNNTGG 1 cut(s) 897
BstYI RGATCY 2 cut(s) 884, 1145
BstZ17I GTATAC 1 cut(s) 871
BsuI GTATCC 1 cut(s) 762
BsuRI GGCC 2 cut(s) 573, 1054
BtsCI GGATG 4 cut(s) 303, 484, 968, 1182
BtsIMutI CAGTG 2 cut(s) 76, 942
Cac8I GCNNGC 2 cut(s) 571, 970
CaiI CAGNNNCTG 1 cut(s) 590
CfoI GCGC 1 cut(s) 513
Cfr13I GGNCC 5 cut(s) 154, 291, 901, 1052, 1072
CseI GACGC 1 cut(s) 1054
Csp6I GTAC 5 cut(s) 267, 668, 715, 811, 1232
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviAII CATG 3 cut(s) 35, 64, 575
CviQI GTAC 5 cut(s) 267, 668, 715, 811, 1232
DdeI CTNAG 2 cut(s) 539, 1139
DpnI GATC 2 cut(s) 886, 1147
DpnII GATC 2 cut(s) 884, 1145
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
Eco31I GGTCTC 1 cut(s) 413
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 4 cut(s) 154, 291, 901, 1072
Eco57I CTGAAG 3 cut(s) 297, 392, 494
Eco91I GGTNACC 1 cut(s) 365
EcoO65I GGTNACC 1 cut(s) 365
EcoRII CCWGG 5 cut(s) 25, 324, 599, 802, 991
EcoRV GATATC 1 cut(s) 856
FaeI CATG 3 cut(s) 38, 67, 578
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 3 cut(s) 473, 914, 1085
FatI CATG 3 cut(s) 34, 63, 574
FauI CCCGC 1 cut(s) 850
FblI GTMKAC 3 cut(s) 682, 870, 1094
Fnu4HI GCNGC 1 cut(s) 588
FokI GGATG 4 cut(s) 290, 491, 975, 1169
Fsp4HI GCNGC 1 cut(s) 588
FspBI CTAG 3 cut(s) 113, 500, 1166
GlaI GCGC 1 cut(s) 512
GluI GCNGC 1 cut(s) 588
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 2 cut(s) 573, 1054
HapII CCGG 2 cut(s) 346, 1151
HgaI GACGC 1 cut(s) 1054
HhaI GCGC 1 cut(s) 513
Hin1II CATG 3 cut(s) 38, 67, 578
Hin6I GCGC 1 cut(s) 511
HinP1I GCGC 1 cut(s) 511
HincII GTYRAC 3 cut(s) 61, 683, 1095
HindII GTYRAC 3 cut(s) 61, 683, 1095
HinfI GANTC 9 cut(s) 175, 239, 399, 662, 695, 737, 838, 1091, 1154
HpaII CCGG 2 cut(s) 346, 1151
HphI GGTGA 2 cut(s) 377, 830
Hpy166II GTNNAC 7 cut(s) 61, 154, 294, 683, 717, 871, 1095
Hpy188I TCNGA 5 cut(s) 82, 277, 407, 474, 1228
Hpy188III TCNNGA 3 cut(s) 392, 984, 1151
Hpy8I GTNNAC 7 cut(s) 61, 154, 294, 683, 717, 871, 1095
Hpy99I CGWCG 1 cut(s) 1070
HpyAV CCTTC 5 cut(s) 367, 469, 953, 990, 1042
HpyCH4III ACNGT 5 cut(s) 91, 533, 905, 1099, 1236
HpyCH4IV ACGT 1 cut(s) 778
HpyCH4V TGCA 9 cut(s) 14, 413, 451, 482, 620, 734, 940, 1120, 1209
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 2 cut(s) 539, 1139
HpySE526I ACGT 1 cut(s) 778
Hsp92II CATG 3 cut(s) 38, 67, 578
HspAI GCGC 1 cut(s) 511
Kpn2I TCCGGA 1 cut(s) 1150
Kzo9I GATC 2 cut(s) 884, 1145
LmnI GCTCC 4 cut(s) 28, 124, 515, 995
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 2 cut(s) 469, 549
MaeI CTAG 3 cut(s) 113, 500, 1166
MaeII ACGT 1 cut(s) 778
MaeIII GTNAC 5 cut(s) 108, 229, 365, 950, 1168
MalI GATC 2 cut(s) 886, 1147
MboI GATC 2 cut(s) 884, 1145
MboII GAAGA 4 cut(s) 199, 604, 652, 734
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 2 cut(s) 884, 1145
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 8 cut(s) 98, 144, 317, 657, 699, 707, 1076, 1211
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 5 cut(s) 184, 393, 746, 1100, 1148
MmeI TCCRAC 2 cut(s) 1068, 1141
MnlI CCTC 7 cut(s) 115, 765, 800, 921, 1043, 1136, 1215
Mox20I TGGCCA 1 cut(s) 573
MroI TCCGGA 1 cut(s) 1150
MscI TGGCCA 1 cut(s) 573
MseI TTAA 4 cut(s) 95, 252, 702, 1214
Msp20I TGGCCA 1 cut(s) 573
MspA1I CMGCKG 3 cut(s) 538, 590, 845
MspI CCGG 2 cut(s) 346, 1151
MspR9I CCNGG 6 cut(s) 27, 326, 346, 601, 804, 993
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 5 cut(s) 27, 326, 601, 804, 993
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 1 cut(s) 20
NciI CCSGG 1 cut(s) 346
NdeII GATC 2 cut(s) 884, 1145
NlaIII CATG 3 cut(s) 38, 67, 578
NlaIV GGNNCC 4 cut(s) 32, 850, 902, 1073
NmuCI GTSAC 2 cut(s) 365, 950
NspI RCATGY 1 cut(s) 67
NspV TTCGAA 1 cut(s) 918
PciI ACATGT 1 cut(s) 63
PfeI GAWTC 4 cut(s) 239, 662, 695, 838
PflFI GACNNNGTC 1 cut(s) 678
PkrI GCNGC 1 cut(s) 589
PleI GAGTC 5 cut(s) 183, 393, 745, 1099, 1148
PpsI GAGTC 5 cut(s) 183, 393, 745, 1099, 1148
PscI ACATGT 1 cut(s) 63
PsiI TTATAA 2 cut(s) 380, 834
Psp6I CCWGG 5 cut(s) 25, 324, 599, 802, 991
PspEI GGTNACC 1 cut(s) 365
PspGI CCWGG 5 cut(s) 25, 324, 599, 802, 991
PspN4I GGNNCC 4 cut(s) 32, 850, 902, 1073
PspPI GGNCC 5 cut(s) 154, 291, 901, 1052, 1072
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 2 cut(s) 884, 1145
PsyI GACNNNGTC 1 cut(s) 678
PvuII CAGCTG 2 cut(s) 538, 590
RsaI GTAC 5 cut(s) 268, 669, 716, 812, 1233
RsaNI GTAC 5 cut(s) 267, 668, 715, 811, 1232
SalI GTCGAC 2 cut(s) 681, 1093
SaqAI TTAA 4 cut(s) 95, 252, 702, 1214
SatI GCNGC 1 cut(s) 588
Sau3AI GATC 2 cut(s) 884, 1145
Sau96I GGNCC 5 cut(s) 154, 291, 901, 1052, 1072
ScaI AGTACT 1 cut(s) 669
SchI GAGTC 5 cut(s) 184, 393, 746, 1100, 1148
ScrFI CCNGG 6 cut(s) 27, 326, 346, 601, 804, 993
SfaNI GCATC 2 cut(s) 469, 549
SfuI TTCGAA 1 cut(s) 918
SinI GGWCC 4 cut(s) 154, 291, 901, 1072
SpeI ACTAGT 1 cut(s) 1165
Sse9I AATT 8 cut(s) 98, 144, 317, 657, 699, 707, 1076, 1211
SsiI CCGC 2 cut(s) 513, 843
SspMI CTAG 3 cut(s) 113, 500, 1166
StyD4I CCNGG 6 cut(s) 25, 324, 344, 599, 802, 991
TaaI ACNGT 5 cut(s) 91, 533, 905, 1099, 1236
TaiI ACGT 1 cut(s) 781
TaqI TCGA 5 cut(s) 441, 682, 918, 985, 1094
TasI AATT 8 cut(s) 98, 144, 317, 657, 699, 707, 1076, 1211
TatI WGTACW 3 cut(s) 667, 714, 1231
TfiI GAWTC 4 cut(s) 239, 662, 695, 838
Tru1I TTAA 4 cut(s) 95, 252, 702, 1214
Tru9I TTAA 4 cut(s) 95, 252, 702, 1214
TscAI CASTG 2 cut(s) 76, 942
TseFI GTSAC 2 cut(s) 365, 950
TseI GCWGC 1 cut(s) 587
Tsp45I GTSAC 2 cut(s) 365, 950
TspDTI ATGAA 2 cut(s) 591, 653
TspGWI ACGGA 1 cut(s) 367
TspRI CASTG 2 cut(s) 76, 942
Tth111I GACNNNGTC 1 cut(s) 678
VpaK11BI GGWCC 4 cut(s) 154, 291, 901, 1072
XapI RAATTY 2 cut(s) 98, 317
XceI RCATGY 1 cut(s) 67
XcmI CCANNNNNNNNNTGG 1 cut(s) 1193
XmiI GTMKAC 3 cut(s) 682, 870, 1094
XspI CTAG 3 cut(s) 113, 500, 1166
ZrmI AGTACT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.