Rh2BG513800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0005889
Physical Location & Seq
Forward (+)
72180548 .. 72185628
5081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG513800.1

Sequence Viewer

Length: 1278 bp
ATGGGAAAAGGACTTATACCTGCTGACCTTGAAACTTGGAAGCAGAGGAGAAGAGTTATTGCTCCTGGGTTCCATGCTGTATACTTGGAAGCTATGGTCAACATGTTCAGTGATTGTTCGGAAAGAACAGTATTAAAATTTGAGAAGTTACTAGAAAGAGAGGAGCTGAATGGGAGAAAGACAATTGAAGTGGACCTTGAAGCAGAGTTTTTGAGTCTGGGGCTTGATATTATTGGGCTTGGTGTCTTCAACTATGACTTTGGTTCTGTTACTAAAGAATCTCCTGTCATTAAGGCAGTATATGGTACTCTCTCTGAAGCTGAACATAGGTCCACTTTTTACATCCCATATTGGAAATTTCCCCTGGCAAGGTGGATTGTTCCCCGGCAACGGAAGTTTTATGGTGACCTGAAGGTTATAAATGATTGTCTTGATGGACTCATCAGAAATGCAAAAGAGACCAGACAGGAAACAGATGTCGAAAAACTGCAACAAAGGGACTACTCTAATCTGAAGGATGCAAGTCTATTGCGTTTCCTAGTTGATATGCGCGGAGCTGATGTTGATGACCGTCAGCTGAGAGATGACTTGATGACGATGCTTATTGCCGGCCATGAAACAACAGCAGCTGTCCTTACCTGGGCTGTTTTCTTCCTTGCACAAAACCCGTCCAAGATGAAGAAAGCACAAACAGAAATTGATTCAGTACTTGGACAAAGTCGACCAACTTTAGATTCAATTAAAAAATTGGAGTACACAAGATTTATTGTTGCGGAGTCTCTTCGTTTGTATCCTCAACCCCCCTTGCTTATTAGACGTTCTCTAAAACCAGATACACTACCAGGAGGGTACAAGGGTGAAAAAGGTGGTTATAAGATTCCCGCCGGAACCGACATCTTTATCTCTGTATACAATCTCCATAGATCTCCATATTACTGGGACCGTCCTAATGTATTCGAACCAGAGAGGTTTTCAGTGCAAAAGAATAGTGACATAGAAGGATGGGCTGGCTTTGACCCTTCTCGAAGCCCTGGAGCATATTATCCAAATGAGATTATGGCTGATTTCTCCTTCTTACCTTTCGGTGGAGGGCCAAGAAAATGCGTCGGGGACCAATTTGCTCTGTTGGAGTCGACTGTATCTTTGGCAATGCTGTTGCAGAAGTTCAATGTAGAGCTAAGAGGATCTCCGGACTCGGTTGGACTAGTAACAGGGGCAACCATCCATACCAAAAATGGAATGTGGTGCAAATTAAGTAAGAGGTCTGATGTACAGTGA

Protein Analysis

425

Amino Acids

48.28

Weight (kDa)

8.15

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 397 1e-70 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 419, 873
Acc36I ACCTGC 1 cut(s) 28
AccI GTMKAC 4 cut(s) 81, 721, 909, 1133
AccII CGCG 1 cut(s) 552
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 3 cut(s) 552, 773, 882
AclWI GGATC 1 cut(s) 1192
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 2 cut(s) 137, 356
AcuI CTGAAG 3 cut(s) 336, 431, 533
AfaI GTAC 5 cut(s) 307, 708, 755, 851, 1272
AfiI CCNNNNNNNGG 4 cut(s) 369, 390, 640, 1085
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 6 cut(s) 32, 188, 200, 250, 738, 1168
AhlI ACTAGT 1 cut(s) 1204
AjnI CCWGG 5 cut(s) 64, 363, 638, 841, 1030
AjuI GAANNNNNNNTTGG 2 cut(s) 718, 750
AluBI AGCT 7 cut(s) 92, 166, 320, 557, 577, 629, 1177
AluI AGCT 7 cut(s) 92, 166, 320, 557, 577, 629, 1177
Alw26I GTCTC 2 cut(s) 452, 783
AlwI GGATC 1 cut(s) 1192
AlwNI CAGNNNCTG 1 cut(s) 629
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 2 cut(s) 610, 1091
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 2 cut(s) 137, 356
ArsI GACNNNNNNTTYG 2 cut(s) 1126, 1158
AspLEI GCGC 1 cut(s) 552
AspS9I GGNCC 5 cut(s) 193, 330, 940, 1091, 1111
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 2 cut(s) 416, 869
AsuII TTCGAA 1 cut(s) 957
AvaII GGWCC 4 cut(s) 193, 330, 940, 1111
BaeI ACNNNNGTAYC 2 cut(s) 841, 874
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 638
BccI CCATC 3 cut(s) 428, 996, 1229
BciT130I CCWGG 5 cut(s) 66, 365, 640, 843, 1032
BciVI GTATCC 1 cut(s) 801
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 452, 783
BcuI ACTAGT 1 cut(s) 1204
BfaI CTAG 3 cut(s) 152, 539, 1205
BfuAI ACCTGC 1 cut(s) 28
BfuI GTATCC 1 cut(s) 801
BglII AGATCT 1 cut(s) 923
BisI GCNGC 1 cut(s) 627
BlsI GCNGC 1 cut(s) 628
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 6 cut(s) 66, 365, 385, 640, 843, 1032
Bme18I GGWCC 4 cut(s) 193, 330, 940, 1111
BmgT120I GGNCC 5 cut(s) 193, 330, 940, 1091, 1111
BmiI GGNNCC 4 cut(s) 71, 889, 941, 1112
BmrFI CCNGG 6 cut(s) 66, 365, 385, 640, 843, 1032
BmrI ACTGGG 1 cut(s) 946
BmsI GCATC 2 cut(s) 508, 588
BmuI ACTGGG 1 cut(s) 946
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 1053
Bpu14I TTCGAA 1 cut(s) 957
BpuMI CCSGG 1 cut(s) 385
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 5 cut(s) 65, 363, 383, 639, 1030
BsaWI WCCGGW 1 cut(s) 1189
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 4 cut(s) 369, 390, 640, 1085
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 1155
BseAI TCCGGA 1 cut(s) 1189
BseBI CCWGG 5 cut(s) 66, 365, 640, 843, 1032
BseDI CCNNGG 5 cut(s) 65, 363, 383, 639, 1030
BseGI GGATG 4 cut(s) 342, 523, 1007, 1221
BseLI CCNNNNNNNGG 4 cut(s) 369, 390, 640, 1085
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 1 cut(s) 569
BseNI ACTGG 1 cut(s) 941
BseRI GAGGAG 2 cut(s) 61, 176
BseXI GCAGC 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 552
BshFI GGCC 2 cut(s) 612, 1093
BsiSI CCGG 4 cut(s) 385, 609, 885, 1190
BslFI GGGAC 3 cut(s) 512, 953, 1124
BslI CCNNNNNNNGG 4 cut(s) 369, 390, 640, 1085
BsmAI GTCTC 2 cut(s) 452, 783
BsmFI GGGAC 3 cut(s) 512, 953, 1124
BsnI GGCC 2 cut(s) 612, 1093
Bso31I GGTCTC 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 957
Bsp13I TCCGGA 1 cut(s) 1189
Bsp1407I TGTACA 1 cut(s) 1270
Bsp143I GATC 2 cut(s) 923, 1184
BspACI CCGC 3 cut(s) 552, 773, 882
BspANI GGCC 2 cut(s) 612, 1093
BspCNI CTCAG 1 cut(s) 570
BspEI TCCGGA 1 cut(s) 1189
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 4 cut(s) 71, 889, 941, 1112
BspMI ACCTGC 1 cut(s) 28
BspPI GGATC 1 cut(s) 1192
BspT104I TTCGAA 1 cut(s) 957
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 1 cut(s) 1155
BsrFI RCCGGY 1 cut(s) 608
BsrGI TGTACA 1 cut(s) 1270
BsrI ACTGG 1 cut(s) 941
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 5 cut(s) 65, 363, 383, 639, 1030
BssMI GATC 2 cut(s) 923, 1184
BssNAI GTATAC 2 cut(s) 82, 910
Bst1107I GTATAC 2 cut(s) 82, 910
Bst2UI CCWGG 5 cut(s) 66, 365, 640, 843, 1032
Bst4CI ACNGT 5 cut(s) 130, 572, 944, 1138, 1275
Bst6I CTCTTC 2 cut(s) 46, 786
BstAUI TGTACA 1 cut(s) 1270
BstBI TTCGAA 1 cut(s) 957
BstC8I GCNNGC 2 cut(s) 610, 1009
BstDEI CTNAG 2 cut(s) 578, 1178
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 4 cut(s) 342, 523, 1007, 1221
BstFNI CGCG 1 cut(s) 552
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 2 cut(s) 926, 1187
BstMAI GTCTC 2 cut(s) 452, 783
BstMBI GATC 2 cut(s) 923, 1184
BstNI CCWGG 5 cut(s) 66, 365, 640, 843, 1032
BstNSI RCATGY 1 cut(s) 106
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 6 cut(s) 64, 363, 383, 638, 841, 1030
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 638
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 2 cut(s) 923, 1184
BstXI CCANNNNNNTGG 1 cut(s) 936
BstYI RGATCY 2 cut(s) 923, 1184
BstZ17I GTATAC 2 cut(s) 82, 910
BsuI GTATCC 1 cut(s) 801
BsuRI GGCC 2 cut(s) 612, 1093
BtsCI GGATG 4 cut(s) 342, 523, 1007, 1221
BtsIMutI CAGTG 2 cut(s) 115, 981
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 610, 1009
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 552
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 5 cut(s) 193, 330, 940, 1091, 1111
CseI GACGC 1 cut(s) 1093
Csp6I GTAC 5 cut(s) 306, 707, 754, 850, 1271
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 3 cut(s) 74, 103, 614
CviQI GTAC 5 cut(s) 306, 707, 754, 850, 1271
DdeI CTNAG 2 cut(s) 578, 1178
DpnI GATC 2 cut(s) 925, 1186
DpnII GATC 2 cut(s) 923, 1184
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 2 cut(s) 46, 786
EarI CTCTTC 2 cut(s) 46, 786
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 4 cut(s) 193, 330, 940, 1111
Eco57I CTGAAG 3 cut(s) 336, 431, 533
Eco91I GGTNACC 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 404
EcoRII CCWGG 5 cut(s) 64, 363, 638, 841, 1030
FaeI CATG 3 cut(s) 77, 106, 617
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 3 cut(s) 512, 953, 1124
FatI CATG 3 cut(s) 73, 102, 613
FauI CCCGC 1 cut(s) 889
FblI GTMKAC 4 cut(s) 81, 721, 909, 1133
Fnu4HI GCNGC 1 cut(s) 627
FokI GGATG 4 cut(s) 329, 530, 1014, 1208
Fsp4HI GCNGC 1 cut(s) 627
FspBI CTAG 3 cut(s) 152, 539, 1205
GlaI GCGC 1 cut(s) 551
GluI GCNGC 1 cut(s) 627
GsuI CTGGAG 1 cut(s) 1053
HaeIII GGCC 2 cut(s) 612, 1093
HapII CCGG 4 cut(s) 385, 609, 885, 1190
HgaI GACGC 1 cut(s) 1093
HhaI GCGC 1 cut(s) 552
Hin1II CATG 3 cut(s) 77, 106, 617
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HincII GTYRAC 3 cut(s) 100, 722, 1134
HindII GTYRAC 3 cut(s) 100, 722, 1134
HinfI GANTC 9 cut(s) 214, 278, 438, 701, 734, 776, 877, 1130, 1193
HpaII CCGG 4 cut(s) 385, 609, 885, 1190
HphI GGTGA 2 cut(s) 416, 869
Hpy166II GTNNAC 8 cut(s) 82, 100, 193, 333, 722, 756, 910, 1134
Hpy188I TCNGA 5 cut(s) 121, 316, 446, 513, 1267
Hpy188III TCNNGA 3 cut(s) 431, 1023, 1190
Hpy8I GTNNAC 8 cut(s) 82, 100, 193, 333, 722, 756, 910, 1134
Hpy99I CGWCG 1 cut(s) 1109
HpyAV CCTTC 5 cut(s) 406, 508, 992, 1029, 1081
HpyCH4III ACNGT 5 cut(s) 130, 572, 944, 1138, 1275
HpyCH4IV ACGT 1 cut(s) 817
HpyCH4V TGCA 7 cut(s) 452, 490, 521, 659, 979, 1159, 1248
HpyF3I CTNAG 2 cut(s) 578, 1178
HpySE526I ACGT 1 cut(s) 817
Hsp92II CATG 3 cut(s) 77, 106, 617
HspAI GCGC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 1189
KroI GCCGGC 1 cut(s) 608
KroNI GCCGGC 1 cut(s) 610
Kzo9I GATC 2 cut(s) 923, 1184
LmnI GCTCC 4 cut(s) 67, 163, 554, 1034
Lsp1109I GCAGC 1 cut(s) 638
LweI GCATC 2 cut(s) 508, 588
MaeI CTAG 3 cut(s) 152, 539, 1205
MaeII ACGT 1 cut(s) 817
MaeIII GTNAC 5 cut(s) 147, 268, 404, 989, 1207
MalI GATC 2 cut(s) 925, 1186
MboI GATC 2 cut(s) 923, 1184
MboII GAAGA 5 cut(s) 63, 238, 643, 691, 773
MfeI CAATTG 1 cut(s) 183
MflI RGATCY 2 cut(s) 923, 1184
MluCI AATT 8 cut(s) 137, 183, 356, 696, 738, 746, 1115, 1250
MlyI GAGTC 5 cut(s) 223, 432, 785, 1139, 1187
MmeI TCCRAC 2 cut(s) 1107, 1180
MnlI CCTC 8 cut(s) 39, 154, 804, 839, 960, 1082, 1175, 1254
MroI TCCGGA 1 cut(s) 1189
MroNI GCCGGC 1 cut(s) 608
MseI TTAA 4 cut(s) 134, 291, 741, 1253
MspA1I CMGCKG 2 cut(s) 577, 629
MspI CCGG 4 cut(s) 385, 609, 885, 1190
MspR9I CCNGG 6 cut(s) 66, 365, 385, 640, 843, 1032
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 5 cut(s) 66, 365, 640, 843, 1032
MvnI CGCG 1 cut(s) 552
NaeI GCCGGC 1 cut(s) 610
NciI CCSGG 1 cut(s) 385
NdeII GATC 2 cut(s) 923, 1184
NgoMIV GCCGGC 1 cut(s) 608
NlaIII CATG 3 cut(s) 77, 106, 617
NlaIV GGNNCC 4 cut(s) 71, 889, 941, 1112
NmuCI GTSAC 2 cut(s) 404, 989
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 957
PciI ACATGT 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 610
PfeI GAWTC 4 cut(s) 278, 701, 734, 877
PflFI GACNNNGTC 1 cut(s) 717
PkrI GCNGC 1 cut(s) 628
PleI GAGTC 5 cut(s) 222, 432, 784, 1138, 1187
PpsI GAGTC 5 cut(s) 222, 432, 784, 1138, 1187
PscI ACATGT 1 cut(s) 102
PsiI TTATAA 2 cut(s) 419, 873
Psp6I CCWGG 5 cut(s) 64, 363, 638, 841, 1030
PspEI GGTNACC 1 cut(s) 404
PspGI CCWGG 5 cut(s) 64, 363, 638, 841, 1030
PspN4I GGNNCC 4 cut(s) 71, 889, 941, 1112
PspPI GGNCC 5 cut(s) 193, 330, 940, 1091, 1111
PstNI CAGNNNCTG 1 cut(s) 629
PsuI RGATCY 2 cut(s) 923, 1184
PsyI GACNNNGTC 1 cut(s) 717
PvuII CAGCTG 2 cut(s) 577, 629
RsaI GTAC 5 cut(s) 307, 708, 755, 851, 1272
RsaNI GTAC 5 cut(s) 306, 707, 754, 850, 1271
SalI GTCGAC 2 cut(s) 720, 1132
SaqAI TTAA 4 cut(s) 134, 291, 741, 1253
SatI GCNGC 1 cut(s) 627
Sau3AI GATC 2 cut(s) 923, 1184
Sau96I GGNCC 5 cut(s) 193, 330, 940, 1091, 1111
ScaI AGTACT 1 cut(s) 708
SchI GAGTC 5 cut(s) 223, 432, 785, 1139, 1187
ScrFI CCNGG 6 cut(s) 66, 365, 385, 640, 843, 1032
SfaNI GCATC 2 cut(s) 508, 588
SfuI TTCGAA 1 cut(s) 957
SinI GGWCC 4 cut(s) 193, 330, 940, 1111
SpeI ACTAGT 1 cut(s) 1204
Sse9I AATT 8 cut(s) 137, 183, 356, 696, 738, 746, 1115, 1250
SsiI CCGC 3 cut(s) 552, 773, 882
SspMI CTAG 3 cut(s) 152, 539, 1205
StyD4I CCNGG 6 cut(s) 64, 363, 383, 638, 841, 1030
TaaI ACNGT 5 cut(s) 130, 572, 944, 1138, 1275
TaiI ACGT 1 cut(s) 820
TaqI TCGA 5 cut(s) 480, 721, 957, 1024, 1133
TasI AATT 8 cut(s) 137, 183, 356, 696, 738, 746, 1115, 1250
TatI WGTACW 3 cut(s) 706, 753, 1270
TfiI GAWTC 4 cut(s) 278, 701, 734, 877
Tru1I TTAA 4 cut(s) 134, 291, 741, 1253
Tru9I TTAA 4 cut(s) 134, 291, 741, 1253
TscAI CASTG 2 cut(s) 115, 981
TseFI GTSAC 2 cut(s) 404, 989
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 2 cut(s) 404, 989
TspDTI ATGAA 2 cut(s) 630, 692
TspGWI ACGGA 1 cut(s) 406
TspRI CASTG 2 cut(s) 115, 981
Tth111I GACNNNGTC 1 cut(s) 717
VpaK11BI GGWCC 4 cut(s) 193, 330, 940, 1111
XapI RAATTY 2 cut(s) 137, 356
XceI RCATGY 1 cut(s) 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 1232
XmiI GTMKAC 4 cut(s) 81, 721, 909, 1133
XspI CTAG 3 cut(s) 152, 539, 1205
ZrmI AGTACT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.