RLG00000023310

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018598
Physical Location & Seq
Reverse (-)
21696406 .. 21699784
3379 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023310

Sequence Viewer

Length: 672 bp
ATGTTGGCGGAGGATCTGAGCAAAAAGATTCGGAAGCACTACACTATTACCAAGTCCAGAGAGAGCTGGACTGAGCCCGAGCACGCTTTGGCTGTGATTGGAATAAGATTGAAGCTTTTATCGAGTCGAAGACAGTTATACAGGCGATACCCTTGGAATCTTGAGGCTGAACTAAAAAGCCAAAGGAAACTAGTTAAAAGTCTAAAGAAAAAGTCACTTTGGTCCAAAAGTATGGAAGAGGTGATGGAAACGCTTGTTGAAATAGTCCACTTTTTGTATCTAGAGATACATGCTGCCTTTGGTAGTGTTGAAAAGGGCACCATAGAAGCACTCAAAGCTTCAGTTACAGAATGCCACCCGTTACTCTTCCAAAGATCTTTACTTTTAACTCTTTCCTCAATCGGAAGCCAGTTTGCTTGGATCAAGAAGCTTCCACCAGCCAAGAAGAAAATAGGGAAGAGTAGGAGACTTGGAAAGAAGCTGCTTAATCTTGTGAAGTTATTCTGGACACTATTGAATCTTCATTACCAGGCGGTTCACCTCAAAATGGAAGAGAGAGTGAAGGAGGGAAGTAAAAACATAGGGGAACATGATCTTCTTGGATGGATTTTGAAGTATTCAAATCTTTCAAAGGAGCAAATTCTTGACTTGATATTGAAGCTGCTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

224

Amino Acids

26.0

Weight (kDa)

9.97

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018598)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0411111
rosa_laevigata RLG00000023310
rosa_multiflora Rmu_sc0008540.1_g000001
rosa_roxburghii Rroxscaffold_2G00109050 Rroxscaffold_2G00109120
rosa_samantha Rh2CG341000 Rh7AG504900 Rh7BG361000 Rh7BG476700
rosa_wichuraiana Rw2G014720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 317
AciI CCGC 2 cut(s) 8, 533
AclWI GGATC 2 cut(s) 21, 428
AcsI RAATTY 1 cut(s) 639
AcuI CTGAAG 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 547
AgsI TTSAA 8 cut(s) 112, 260, 311, 517, 613, 621, 630, 658
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 1 cut(s) 528
AluBI AGCT 6 cut(s) 66, 115, 338, 430, 481, 661
AluI AGCT 6 cut(s) 66, 115, 338, 430, 481, 661
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 2 cut(s) 21, 428
Ama87I CYCGRG 1 cut(s) 77
ApeKI GCWGC 3 cut(s) 293, 481, 661
ApoI RAATTY 1 cut(s) 639
Asp700I GAANNNNTTC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 253, 530
AvaI CYCGRG 1 cut(s) 77
AvaII GGWCC 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 78
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 3 cut(s) 280, 468, 648
BccI CCATC 2 cut(s) 238, 597
BciT130I CCWGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 460
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 2 cut(s) 191, 281
BglII AGATCT 1 cut(s) 374
BisI GCNGC 3 cut(s) 294, 482, 662
BlsI GCNGC 3 cut(s) 295, 483, 663
Bme1390I CCNGG 1 cut(s) 530
Bme18I GGWCC 1 cut(s) 222
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 222
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 152
Bsc4I CCNNNNNNNGG 1 cut(s) 547
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 1 cut(s) 152
BseGI GGATG 1 cut(s) 608
BseLI CCNNNNNNNGG 1 cut(s) 547
BseMII CTCAG 2 cut(s) 8, 63
BseNI ACTGG 1 cut(s) 409
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 3 cut(s) 280, 468, 648
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 547
BsmAI GTCTC 1 cut(s) 460
BsmI GAATGC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 77
Bsp1286I GDGCHC 3 cut(s) 78, 84, 320
Bsp143I GATC 4 cut(s) 13, 374, 420, 592
BspACI CCGC 2 cut(s) 8, 533
BspCNI CTCAG 2 cut(s) 9, 64
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 2 cut(s) 21, 428
BspT107I GGYRCC 1 cut(s) 317
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 4 cut(s) 13, 374, 420, 592
BssT1I CCWWGG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 4 cut(s) 231, 371, 452, 546
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 17, 72
BstF5I GGATG 1 cut(s) 608
BstKTI GATC 4 cut(s) 16, 377, 423, 595
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 4 cut(s) 13, 374, 420, 592
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 530
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 1 cut(s) 528
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 3 cut(s) 280, 468, 648
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 2 cut(s) 13, 374
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 2 cut(s) 13, 374
BtsCI GGATG 1 cut(s) 608
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 222
CviAII CATG 2 cut(s) 290, 590
DdeI CTNAG 2 cut(s) 17, 72
DpnI GATC 4 cut(s) 15, 376, 422, 594
DpnII GATC 4 cut(s) 13, 374, 420, 592
Eam1104I CTCTTC 4 cut(s) 231, 371, 452, 546
EarI CTCTTC 4 cut(s) 231, 371, 452, 546
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 152
EcoT38I GRGCYC 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 2 cut(s) 293, 593
FaiI YATR 6 cut(s) 139, 233, 291, 323, 581, 591
FalI AAGNNNNNCTT 1 cut(s) 650
FatI CATG 2 cut(s) 289, 589
Fnu4HI GCNGC 3 cut(s) 294, 482, 662
FokI GGATG 1 cut(s) 615
FriOI GRGCYC 1 cut(s) 78
Fsp4HI GCNGC 3 cut(s) 294, 482, 662
FspBI CTAG 2 cut(s) 191, 281
GluI GCNGC 3 cut(s) 294, 482, 662
Hin1II CATG 2 cut(s) 293, 593
HindIII AAGCTT 3 cut(s) 113, 336, 428
HinfI GANTC 4 cut(s) 28, 124, 157, 517
HphI GGTGA 2 cut(s) 253, 530
Hpy166II GTNNAC 2 cut(s) 268, 538
Hpy188I TCNGA 4 cut(s) 18, 33, 404, 671
Hpy188III TCNNGA 6 cut(s) 57, 161, 281, 424, 505, 644
Hpy8I GTNNAC 2 cut(s) 268, 538
HpyAV CCTTC 1 cut(s) 556
HpyCH4III ACNGT 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 2 cut(s) 17, 72
Hsp92II CATG 2 cut(s) 293, 593
Kzo9I GATC 4 cut(s) 13, 374, 420, 592
LmnI GCTCC 1 cut(s) 634
LpnPI CCDG 8 cut(s) 52, 70, 127, 422, 450, 490, 515, 542
Lsp1109I GCAGC 3 cut(s) 280, 468, 648
MaeI CTAG 2 cut(s) 191, 281
MaeIII GTNAC 3 cut(s) 213, 343, 360
MalI GATC 4 cut(s) 15, 376, 422, 594
MboI GATC 4 cut(s) 13, 374, 420, 592
MboII GAAGA 9 cut(s) 141, 248, 358, 457, 469, 512, 563, 587, 658
MflI RGATCY 2 cut(s) 13, 374
MhlI GDGCHC 3 cut(s) 78, 84, 320
MluCI AATT 1 cut(s) 639
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 6 cut(s) 4, 157, 232, 406, 551, 559
MroXI GAANNNNTTC 1 cut(s) 500
MseI TTAA 3 cut(s) 195, 386, 486
MspR9I CCNGG 1 cut(s) 530
Mva1269I GAATGC 1 cut(s) 356
MvaI CCWGG 1 cut(s) 530
MwoI GCNNNNNNNGC 1 cut(s) 335
NdeII GATC 4 cut(s) 13, 374, 420, 592
NlaIII CATG 2 cut(s) 293, 593
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 213
NspI RCATGY 1 cut(s) 293
PctI GAATGC 1 cut(s) 356
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 3 cut(s) 28, 157, 517
PkrI GCNGC 3 cut(s) 295, 483, 663
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 528
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 222
PsuI RGATCY 2 cut(s) 13, 374
SaqAI TTAA 3 cut(s) 195, 386, 486
SatI GCNGC 3 cut(s) 294, 482, 662
Sau3AI GATC 4 cut(s) 13, 374, 420, 592
Sau96I GGNCC 1 cut(s) 222
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 530
SduI GDGCHC 3 cut(s) 78, 84, 320
SetI ASST 8 cut(s) 68, 117, 243, 340, 432, 483, 543, 663
SinI GGWCC 1 cut(s) 222
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 1 cut(s) 639
SsiI CCGC 2 cut(s) 8, 533
SspMI CTAG 2 cut(s) 191, 281
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 122, 127
TasI AATT 1 cut(s) 639
TfiI GAWTC 3 cut(s) 28, 157, 517
Tru1I TTAA 3 cut(s) 195, 386, 486
Tru9I TTAA 3 cut(s) 195, 386, 486
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 3 cut(s) 293, 481, 661
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 222
XapI RAATTY 1 cut(s) 639
XbaI TCTAGA 1 cut(s) 280
XceI RCATGY 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 2 cut(s) 191, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.