RLG00000024793

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017050
Physical Location & Seq
Forward (+)
39165007 .. 39165654
648 bp
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UTR
Exon/CDS
Intron
RLM00000024793

Sequence Viewer

Length: 567 bp
ATGTCATCGTTCGGATCCGGGTCTACCCGCCCCTTCGACGACGGAGAGACTCAATCTCCTCCGTCGATATTTTCAGCCGGCGGTGGATTTGGAAGCTCCGAGGCGGAGGAGGGGCTTGCCCTGAGAGAGTGGCGGAGGCGGAATGCGATTGAGCTGGAGGAGAAGGAGAAGAAGGAGAAAGAGAAGCTGAAGCAGATAATAGAGGAAGCGGAGGCGTTCAAAGTGGAGTTCTACAGGAAGCGGAAGCTGAACGTGGAGAAGAAGAAGGTTTCGAACAGAGAAGCCGAGAGGGTATTTCTGGAAAATCAGGAGAAGTTTCATGCAGAAGCTGAGAGGAATTACTGGAAAGCAGTTGCGGAGATGATTCCTCGTGAGGTTGCAGCGATTGAGAAGAGGGGGAAGAGAGAGAAGGAGAAGAAGACGGGCTCTGTGGTTGTGATTCATGGGCCGAAGCCTGGGAAGCCCACCGATCTTTCGAGGATGCGTCAGATTCTGGTGAAGCTGAAGCAGAGTCCTCCTGCTCACTGGAACCCTAAGCCAGAGGAGCTGGCTGTGGCTCATGCTTAG

Protein Analysis

189

Amino Acids

21.48

Weight (kDa)

9.47

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 33 - 172 1.2e-09 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017050)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51890
prunus_persica Prupe.6G275500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0463291
rosa_laevigata RLG00000024793
rosa_multiflora Rmu_co8110798.1_g000001 Rmu_sc0002976.1_g000007
rosa_roxburghii Rroxscaffold_6G00416700
rosa_rugosa Rorug03G0063600
rosa_samantha Rh3AG121500 Rh3BG126000 Rh3CG127900 Rh3DG127100
rosa_wichuraiana Rw3G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 23
AciI CCGC 8 cut(s) 28, 81, 104, 133, 139, 209, 241, 356
AclWI GGATC 2 cut(s) 9, 22
AcuI CTGAAG 2 cut(s) 209, 524
AfiI CCNNNNNNNGG 1 cut(s) 455
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 7 cut(s) 96, 154, 187, 247, 329, 502, 547
AluI AGCT 7 cut(s) 96, 154, 187, 247, 329, 502, 547
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 2 cut(s) 9, 22
AlwNI CAGNNNCTG 2 cut(s) 329, 493
AoxI GGCC 1 cut(s) 446
ApeKI GCWGC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 446
AsuC2I CCSGG 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 508
AsuII TTCGAA 1 cut(s) 272
BamHI GGATCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 428
BauI CACGAG 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 392
BciT130I CCWGG 1 cut(s) 456
BcnI CCSGG 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 232
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
Bme1390I CCNGG 2 cut(s) 19, 456
BmgT120I GGNCC 1 cut(s) 446
BmiI GGNNCC 2 cut(s) 16, 530
BmrFI CCNGG 2 cut(s) 19, 456
BmsI GCATC 1 cut(s) 471
BpiI GAAGAC 1 cut(s) 425
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 99, 455
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 347, 530
BseBI CCWGG 1 cut(s) 456
BseDI CCNNGG 2 cut(s) 99, 455
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMII CTCAG 2 cut(s) 113, 321
BseNI ACTGG 2 cut(s) 347, 530
BseRI GAGGAG 4 cut(s) 48, 122, 173, 557
BseXI GCAGC 1 cut(s) 392
BshFI GGCC 1 cut(s) 448
BsiSI CCGG 2 cut(s) 18, 78
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 1 cut(s) 41
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 1 cut(s) 448
Bsp119I TTCGAA 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 428
Bsp143I GATC 2 cut(s) 14, 469
BspACI CCGC 8 cut(s) 28, 81, 104, 133, 139, 209, 241, 356
BspANI GGCC 1 cut(s) 448
BspCNI CTCAG 2 cut(s) 114, 322
BspLI GGNNCC 2 cut(s) 16, 530
BspPI GGATC 2 cut(s) 9, 22
BspT104I TTCGAA 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 2 cut(s) 347, 530
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 99, 455
BssMI GATC 2 cut(s) 14, 469
BssSI CACGAG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 456
Bst6I CTCTTC 2 cut(s) 386, 395
BstBI TTCGAA 1 cut(s) 272
BstC8I GCNNGC 3 cut(s) 79, 117, 549
BstDEI CTNAG 4 cut(s) 122, 330, 534, 564
BstF5I GGATG 1 cut(s) 486
BstKTI GATC 2 cut(s) 17, 472
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 2 cut(s) 14, 469
BstMWI GCNNNNNNNGC 2 cut(s) 460, 544
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 2 cut(s) 17, 454
BstSFI CTRYAG 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
BsuRI GGCC 1 cut(s) 448
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 523
Cac8I GCNNGC 3 cut(s) 79, 117, 549
CaiI CAGNNNCTG 2 cut(s) 329, 493
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 446
CseI GACGC 1 cut(s) 473
CviAII CATG 3 cut(s) 320, 443, 560
DdeI CTNAG 4 cut(s) 122, 330, 534, 564
DpnI GATC 2 cut(s) 16, 471
DpnII GATC 2 cut(s) 14, 469
Eam1104I CTCTTC 2 cut(s) 386, 395
EarI CTCTTC 2 cut(s) 386, 395
EciI GGCGGA 3 cut(s) 119, 148, 154
Eco24I GRGCYC 1 cut(s) 428
Eco57I CTGAAG 2 cut(s) 209, 524
EcoRII CCWGG 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 428
FaeI CATG 3 cut(s) 323, 446, 563
FaiI YATR 3 cut(s) 321, 444, 561
FatI CATG 3 cut(s) 319, 442, 559
FauI CCCGC 1 cut(s) 35
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 1 cut(s) 493
FriOI GRGCYC 1 cut(s) 428
Fsp4HI GCNGC 1 cut(s) 381
GluI GCNGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 448
HapII CCGG 2 cut(s) 18, 78
HgaI GACGC 1 cut(s) 473
Hin1II CATG 3 cut(s) 323, 446, 563
HinfI GANTC 5 cut(s) 49, 364, 439, 490, 511
HpaII CCGG 2 cut(s) 18, 78
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 14, 100, 489
Hpy188III TCNNGA 3 cut(s) 299, 308, 371
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 3 cut(s) 41, 44, 67
HpyAV CCTTC 5 cut(s) 43, 157, 166, 259, 403
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 2 cut(s) 323, 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 460, 544
HpyF3I CTNAG 4 cut(s) 122, 330, 534, 564
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 3 cut(s) 323, 446, 563
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 14, 469
LmnI GCTCC 2 cut(s) 101, 544
Lsp1109I GCAGC 1 cut(s) 392
LweI GCATC 1 cut(s) 471
MaeII ACGT 1 cut(s) 252
MalI GATC 2 cut(s) 16, 471
MboI GATC 2 cut(s) 14, 469
MboII GAAGA 7 cut(s) 181, 271, 274, 403, 412, 427, 430
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 1 cut(s) 337
MlyI GAGTC 2 cut(s) 43, 520
MroNI GCCGGC 1 cut(s) 77
MspI CCGG 2 cut(s) 18, 78
MspR9I CCNGG 2 cut(s) 19, 456
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 460, 544
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 1 cut(s) 19
NdeII GATC 2 cut(s) 14, 469
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 3 cut(s) 323, 446, 563
NlaIV GGNNCC 2 cut(s) 16, 530
NmeAIII GCCGAG 1 cut(s) 310
NspV TTCGAA 1 cut(s) 272
PctI GAATGC 1 cut(s) 148
PdiI GCCGGC 1 cut(s) 79
PfeI GAWTC 3 cut(s) 364, 439, 490
PkrI GCNGC 1 cut(s) 382
PleI GAGTC 2 cut(s) 43, 519
PpsI GAGTC 2 cut(s) 43, 519
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 2 cut(s) 16, 530
PspPI GGNCC 1 cut(s) 446
PstNI CAGNNNCTG 2 cut(s) 329, 493
PsuI RGATCY 1 cut(s) 14
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 2 cut(s) 14, 469
Sau96I GGNCC 1 cut(s) 446
SchI GAGTC 2 cut(s) 43, 520
ScrFI CCNGG 2 cut(s) 19, 456
SduI GDGCHC 1 cut(s) 428
SfaNI GCATC 1 cut(s) 471
SfcI CTRYAG 1 cut(s) 232
SfuI TTCGAA 1 cut(s) 272
Sse9I AATT 1 cut(s) 337
SsiI CCGC 8 cut(s) 28, 81, 104, 133, 139, 209, 241, 356
StyD4I CCNGG 2 cut(s) 17, 454
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 36, 65, 272, 476
TasI AATT 1 cut(s) 337
TfiI GAWTC 3 cut(s) 364, 439, 490
TscAI CASTG 1 cut(s) 530
TseI GCWGC 1 cut(s) 380
TspDTI ATGAA 2 cut(s) 308, 431
TspGWI ACGGA 2 cut(s) 51, 57
TspRI CASTG 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.