RLG00000025117

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017389
Physical Location & Seq
Forward (+)
42076807 .. 42077794
988 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025117

Sequence Viewer

Length: 624 bp
ATGAGAGCATTGAAGAACCCAAGAAGCCCGGTCGCCGTGGCCGGAAAAAGACCCCACCAAAGAAAACCCAGCGCTCCAAAAAAGCCCAGATTGAAAAATGGCCCGATAGAACTAGAACCCGAATCTTCAGATTCGGAGCTCAAAGATTACGACAACGGCATCGATTTCCTGTACGACTACCCGCCCTTGGTTTGCTGCTTTAGGGCAGCCCAGAAAAAGTTTGTCCCGACGGTTCGGGTCTCTGACAACCCGATGGACCTAGACATGCCCAATGCTTACTCTTACAGTGTTATCATCAAGGCACTGGTTAAGGACAAAGACCCGGATTTTCTTGGTGTAGCAAAGGAGTGTGTGATGGAGATGATGGGCAGGGGAATGCAGCCCAATGCTGGTACATACGTGGCGGTTTCTGAGGGGTTTGTTGAGAAGGAGAAGGCTGAGGAGGGCAGGGAGCTTGTGGAGGTGATGAAAGCTAAGGGCTTTGTGGCGGATAAGAAGGCGGTGAGGGAGGTTCTTAAAGGCACAAGAGGAGTTTTGGCTGACAAGGTCATCAAGATGGTCTTTGGAGTCTTTGGTAAACGTTGGATGAAAGTCTTGATACTAGTGAAATCAAAAGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

208

Amino Acids

23.07

Weight (kDa)

9.5

Isoelectric Point (pI)

32.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 182, 404, 488, 500
AclI AACGTT 1 cut(s) 580
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 2 cut(s) 173, 394
AfeI AGCGCT 1 cut(s) 73
AfiI CCNNNNNNNGG 2 cut(s) 187, 389
AgsI TTSAA 2 cut(s) 13, 94
AhlI ACTAGT 1 cut(s) 601
AluBI AGCT 5 cut(s) 139, 454, 473, 617, 621
AluI AGCT 5 cut(s) 139, 454, 473, 617, 621
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 244
Aor51HI AGCGCT 1 cut(s) 73
AoxI GGCC 2 cut(s) 39, 100
ApeKI GCWGC 3 cut(s) 195, 206, 379
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 2 cut(s) 101, 256
AsuC2I CCSGG 2 cut(s) 29, 323
AsuHPI GGTGA 2 cut(s) 475, 514
AsuNHI GCTAGC 1 cut(s) 617
AvaII GGWCC 1 cut(s) 256
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BanII GRGCYC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 182, 218, 391
BccI CCATC 4 cut(s) 247, 349, 358, 550
BceAI ACGGC 2 cut(s) 20, 172
BcnI CCSGG 2 cut(s) 29, 323
BcoDI GTCTC 1 cut(s) 244
BcuI ACTAGT 1 cut(s) 601
BfaI CTAG 5 cut(s) 113, 260, 602, 618, 622
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 3 cut(s) 196, 207, 380
BlsI GCNGC 3 cut(s) 197, 208, 381
Bme1390I CCNGG 2 cut(s) 29, 323
Bme18I GGWCC 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 101, 256
BmrFI CCNGG 2 cut(s) 29, 323
BmsI GCATC 1 cut(s) 168
BmtI GCTAGC 1 cut(s) 621
Bpu10I CCTNAGC 2 cut(s) 438, 474
BpuMI CCSGG 2 cut(s) 29, 323
Bsa29I ATCGAT 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 400
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 2 cut(s) 36, 186
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 389
Bse1I ACTGG 1 cut(s) 309
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 36, 186
BseGI GGATG 1 cut(s) 591
BseLI CCNNNNNNNGG 2 cut(s) 187, 389
BseMII CTCAG 2 cut(s) 402, 429
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 2 cut(s) 455, 543
BseXI GCAGC 3 cut(s) 182, 218, 391
BseYI CCCAGC 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 41, 102
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 3 cut(s) 29, 42, 323
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 2 cut(s) 187, 389
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 2 cut(s) 41, 102
Bso31I GGTCTC 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 141
BspACI CCGC 4 cut(s) 182, 404, 488, 500
BspANI GGCC 2 cut(s) 41, 102
BspCNI CTCAG 2 cut(s) 403, 430
BspDI ATCGAT 1 cut(s) 162
BspOI GCTAGC 1 cut(s) 621
BspTNI GGTCTC 1 cut(s) 244
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 36, 186
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 232, 287
BstBAI YACGTR 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 3 cut(s) 411, 438, 474
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 591
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstMAI GTCTC 1 cut(s) 244
BstMCI CGRYCG 1 cut(s) 33
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 2 cut(s) 27, 321
BstV1I GCAGC 3 cut(s) 182, 218, 391
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 2 cut(s) 41, 102
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 591
BtsIMutI CAGTG 2 cut(s) 292, 302
Cac8I GCNNGC 1 cut(s) 619
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 101, 256
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 2 cut(s) 172, 393
CviAII CATG 1 cut(s) 265
CviQI GTAC 2 cut(s) 172, 393
DdeI CTNAG 3 cut(s) 411, 438, 474
EaeI YGGCCR 1 cut(s) 39
EciI GGCGGA 1 cut(s) 503
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 141
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 256
Eco47III AGCGCT 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 186
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 1 cut(s) 268
FaiI YATR 2 cut(s) 266, 397
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FaqI GGGAC 1 cut(s) 209
FatI CATG 1 cut(s) 264
FauI CCCGC 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 196, 207, 380
FokI GGATG 1 cut(s) 598
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 3 cut(s) 196, 207, 380
FspBI CTAG 5 cut(s) 113, 260, 602, 618, 622
GlaI GCGC 1 cut(s) 73
GluI GCNGC 3 cut(s) 196, 207, 380
GsaI CCCAGC 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 41, 102
HapII CCGG 3 cut(s) 29, 42, 323
HhaI GCGC 1 cut(s) 74
Hin1II CATG 1 cut(s) 268
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 3 cut(s) 122, 131, 567
HpaII CCGG 3 cut(s) 29, 42, 323
HphI GGTGA 2 cut(s) 475, 514
Hpy166II GTNNAC 1 cut(s) 578
Hpy188I TCNGA 4 cut(s) 130, 136, 244, 412
Hpy188III TCNNGA 3 cut(s) 226, 553, 595
Hpy8I GTNNAC 1 cut(s) 578
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 3 cut(s) 421, 427, 490
HpyCH4III ACNGT 2 cut(s) 232, 287
HpyCH4IV ACGT 2 cut(s) 399, 580
HpyCH4V TGCA 1 cut(s) 379
HpyF3I CTNAG 3 cut(s) 411, 438, 474
HpySE526I ACGT 2 cut(s) 399, 580
Hsp92II CATG 1 cut(s) 268
HspAI GCGC 1 cut(s) 72
LmnI GCTCC 3 cut(s) 79, 136, 451
Lsp1109I GCAGC 3 cut(s) 182, 218, 391
LweI GCATC 1 cut(s) 168
MaeI CTAG 5 cut(s) 113, 260, 602, 618, 622
MaeII ACGT 2 cut(s) 399, 580
MboII GAAGA 2 cut(s) 25, 117
MhlI GDGCHC 1 cut(s) 141
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 7 cut(s) 406, 433, 436, 454, 498, 502, 521
MseI TTAA 2 cut(s) 309, 516
MslI CAYNNNNRTG 1 cut(s) 554
MspI CCGG 3 cut(s) 29, 42, 323
MspR9I CCNGG 2 cut(s) 29, 323
Mva1269I GAATGC 1 cut(s) 381
NciI CCSGG 2 cut(s) 29, 323
NheI GCTAGC 1 cut(s) 617
NlaIII CATG 1 cut(s) 268
NspI RCATGY 1 cut(s) 268
PctI GAATGC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 122, 131
PflFI GACNNNGTC 1 cut(s) 545
PkrI GCNGC 3 cut(s) 197, 208, 381
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
Ppu21I YACGTR 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 141
Psp1406I AACGTT 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 68
PspPI GGNCC 2 cut(s) 101, 256
PsyI GACNNNGTC 1 cut(s) 545
RsaI GTAC 2 cut(s) 173, 394
RsaNI GTAC 2 cut(s) 172, 393
RseI CAYNNNNRTG 1 cut(s) 554
SacI GAGCTC 1 cut(s) 141
SaqAI TTAA 2 cut(s) 309, 516
SatI GCNGC 3 cut(s) 196, 207, 380
Sau96I GGNCC 2 cut(s) 101, 256
SchI GAGTC 1 cut(s) 576
ScrFI CCNGG 2 cut(s) 29, 323
SduI GDGCHC 1 cut(s) 141
SfaNI GCATC 1 cut(s) 168
SinI GGWCC 1 cut(s) 256
SmiMI CAYNNNNRTG 1 cut(s) 554
SpeI ACTAGT 1 cut(s) 601
SsiI CCGC 4 cut(s) 182, 404, 488, 500
SspMI CTAG 5 cut(s) 113, 260, 602, 618, 622
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 27, 321
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 232, 287
TaiI ACGT 2 cut(s) 402, 583
TaqI TCGA 1 cut(s) 162
TfiI GAWTC 2 cut(s) 122, 131
Tru1I TTAA 2 cut(s) 309, 516
Tru9I TTAA 2 cut(s) 309, 516
TscAI CASTG 2 cut(s) 292, 309
TseI GCWGC 3 cut(s) 195, 206, 379
TspDTI ATGAA 2 cut(s) 482, 602
TspRI CASTG 2 cut(s) 292, 309
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 256
XceI RCATGY 1 cut(s) 268
XspI CTAG 5 cut(s) 113, 260, 602, 618, 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.