Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0010880

Physical Location & Seq

Genomic Coordinates Forward (+)

48811484 .. 48823852

Gene Structure 12369 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000025868

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3870 bp

ATGGCTGTTCCGGTTGAAGAAGCCATTGCTGCGCTATCTACATTCTCACTGGAGGATGAGCAAGCAGAGGTTCAAGGACCAGGAATTTGGGTTTCGACTGACACCGGAGCAACTGACAGTCCAATTGACTATAGCGATGTTTCTGCATATCGCTTGTCTTTATCTGAAGACACAAAAGCTCTCAATCAACTGAATGCTCTTATACTCGAGGGAAAGGAGATGGGATCTGTGCTGTATACATATCGTAGTTGTGTCAAGGCACTTCCTCAGCTTCCCGATTCTATGAAACAGAATCAAGCTGACTTGTACCTTGAAACTTATCAAGTTCTGGACTTGGAAATGAGTCGCCTACGTGAAATACAGCGCTGGCAAGCTTCGGCTGCATCAAAGCTAGCTGCAGATATGCAACGCTTCTCAAGGCCCGAGAGACGCATTAATGGACCTACAGTAACTCATCTCTGGTCCATGTTGAAGTTACTTGACACTTTGGTGCAACTTGATCATCTTAAGAATGCAAAGGCAAGCATACCAAATGATTTCTCTTGGTATAAAAGGACATTCACACAAGTAAGTGTACAGTGGCATGATACTGATTCAATGAGAGAGGAGTTGGATGACTTACAGATTTTCCTTAGCACAAGGTGGGCTATCTTGTTGAATTTGCATGCTGAGATGTTCCGTGTGAACAACGTGGAAGATATTCTTCAAGTACTCATAGTTTTTGCTGTCGAGTCACTAGAGCTGGATTTTGCACTTCTGTTCCCAGAGCGGCACATTCTTCTTCGTGTTCTGCCGATTCTAGTTGTCTTAGCAACATCGTCAGAAAAAGATAGCGAGTCTTTGTACAAGAGAGTGAAAATCAACAGGCTTATTAACATATTCAAGAATGATCCAGTAATTCCTGCATTTCCAGATCTCCATCTTTCTCCTGCTGCAATCATGAAAGAATTGTCAATGTACTTCCAAAAGTTTTCTGCCCAAACTCGTCTTCTCAGCCTTCCATCACCTCATGAGCTTCCAGCCCGTGAGGCACAAGAATATCCTTTGTTTATCTTTTTTAGGCATTATCTAATCGTCAATCATATTGGATCCATCCGTGCCGAACATGATGACTTCACAATTCGTTTTGCTTCCTCCATGAATCAGCTATTGTTGCTGAAGTCAGCAGATAGTGCCGATATTGAGTGGTGCAAAGAAGTCAAGGGAAATATATATGATATGATTGTTGAAGGTTTTCAGCTTTTAAGTAGATGGACTGCACGCATTTGGGAACAATGTGCATGGAAATTTTCTCGTCCCTGCAAGGATATAGTTCCTTCCGAATCACAAGAGGCTTCAGCATCATTTTCTGACTATGAAAAGGTGGTACGTTATAACTATAGTGCAGATGAAAGGAAAGCGCTGGTGGAACTTGTTAGCTACATAAAGAGCATAGGATCTATGATGCAGTCTAGTGACACATTGGTAGCTGATGCTTTATGGGAGACAATACATGCCGAGGTTCAAGATTTTGTTCAGAATACATTGGCCACTATGTTGCGTACTACCTTTCGAAAGAAGAAGGACCTTTCTAGGATCCTTTCCGATATGCGGACTCTTTCAGCAGACTGGATGGCAAATACAAGTAAGTCTGAATCTGGACCCCTTCAGCATGGTAGTGAAGAAAGCAAAGGAAATGGTTTTTATCCAAGGCCAGTTGCACCAACAGCCGCTCAGGTGCACTGCTTGCAGTTCCTGATATATGAAGTTGTTTCCGGTGGTAACCTTAGGAAGCCTGGAGGGCTCTTTGGGAATGGTGGTTCTGAGATCCCTGTTAATGACCTAAAACAGTTAGAAACATTTTTTTACAAGCTTAGCTTCTTCCTGCATATTCTGGACTATTCAGCGACTGCCGCAACGTTGACGGATCTTGGTTTCTTATGGTTTAGAGAATTTTATTTGGAGTCTTCTCGTGTTATTCAGTTTCCGATCGAATGCTCTCTTCCTTGGATGTTGGTGGATTATGTGCTTGAGTCACATAATGCCGGTATTCTAGAGAGTGTTTTGATACCATTTGACATCTATAACGATTCTGCTCAGCAAGCATTGGTTGTGCTCAAGCAACGGTTCCTCTATGATGAAATTGAGGCTGAGGTGGATCATTGTTTCGACATATTTGTTTCAAAACTCTGTGATACAATTTTCACATATTACAAGAGCTGGGCAGCAAGCAAGCTACTTGATATGTCATTCCTTTTCGCATTGGACAATGGAGAAAGATACTCTGTCGAGCCTATGAGGTTCACAACACTGCTAAAGATGACCAGAGTGAAGTTGCTTGGAAGGATGATTGATTTAAGGAGTTTGATTACTGAGCGGATGAACAAAGTTTTCAGAGATAATATTGAGTTTCTATATGATCGTTTTGAGTCTCAAGATCTATGTGCTATTGTGGAACTAGAAAATCTGCTGGATATCTTAAAGCATGCCCATGAATCGCTCTCCAGAGATCTCTCAATAGACTCATTCAGTCTAATGTTGAATGAGATGCAAGAAAATATATCACTGGTGTCATATTCTAGTAGACTTGCCTCTCAGATTTGGTCAGAGATGCAGAATGATTTCTTGCCAAACTTCATCCTTTGCAATACTACCCAGCGTTTTACACGATCAGCAAAGGTTCCTCTTGTTCCTGTTCAAAAGCCATCAGTTCCCTACGCCAAGCCTAATTTCTATTGCGGTACTCAAGATTTAAATGCAGCTCATCAAAGTTTTGCAAGGTTGCACAGTGGATTTTTTGGAATGCCTCACATGTTTTCCATTGTAAGGCTTCTAGGGTCCAGATCATTGCCCTGGCTAATCCGAGCACTTCTAGATCATATATCTAACAAGACAGCTACCCTGGAACCCTTGATAACAGGATTGCAAGAAGCATTGCCAAAATCTATTGGACTGCTCCCTTTTGATGGAGGCGTGACAGGTTGTATGAGGCTTGTTAAGGAACAGCTTACTTGGGGAACCAAATCAGAGCTCAAAGGAGAAGTTCTTCGGGGTATAAAGGAGATTGGAAGCGTATTATATTGGCTGGGGCTTCTTGATATCGTATTGAGAGAAACAGACACCACACATTTCATGCAAACAGCCCCTTGGTTGGGCTTGCTTCCTGCTGCAGATGGACAAATATTACATTCTCAAGATGGTGGAGAAAGCCCTATTGTCAACCTTTTTAAATCAGCTACTTCTGCTATTGTGTCAAACCCTGGATGTCCAAATCCGGCTTCCTTCAATACCTTATCGAAGCAGGCAGAAGCTGCAGATCTTTTGTACAAGGCTAACATGAACACGGGAAGTGTGCTGGAATATTCTCTAGCTTTTACTAGTGCAGCACTGGATAAATACTGTAGTAAATGGAGTGCTGTTCCCAAAACAGGGTTTATTGATATCACAACTTCAAAGGATTTCTATCGTATATACAGTGGCCTTCAAATTTGGTATCTAGAGGAGTCTGTTCGAGTATTGCCAAACAATCAAGAAGTGCTGGGTGATTCTGTAGCTTGGGGTGGTTGCACCATAATCTACTTGCTTGGGCAACAGCTGCATTTCGAGCTCTTGGATTTTTCATATCAGGTCCTCAATGTTGCTGAAGTGGAGGCTGCATCAATTACCCAAACCCATAAGAGCCCTCATTATGTTCAGGGATGGGATGGCCTGTTAGAAGTAATGAAGAAAGCAAGGAGGTTGAACAACCATGTGTTCTCCATGTTAAAGGCGCGTTGCCCTCTTGAAGATAAGACAGCTTGTGCTATCAAGCAAAGTGGTGCCCCTCTGCACCGGATCAAGTTTGAGAACACCGTCTCCGCATTTGAAACACTGCCACAGAAAGAGGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010638 GO:0016043 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031334 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0043254 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0045010 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko03013 ko04810 map03013 map04810

Pfam Domains

Protein Families

DUF1394 FragX_IP

Protein Analysis

1290

Amino Acids

146.21

Weight (kDa)

5.53

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FragX_IP	PF05994	355 - 1250	0.0	Cytoplasmic Fragile-X interacting family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010880)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18410 AT5G18410 AT5G18410
fragaria_vesca	FvH4_6g01330 FvH4_6g01330 FvH4_6g01330
malus_domestica	MD12G1252900.v1.1
prunus_persica	Prupe.6G354800_v2.0.a1
pyrus_communis	pycom12g23200
rosa_chinensis	RchiOBHm_Chr3g0448901
rosa_laevigata	RLG00000025868
rosa_multiflora	Rmu_sc0003116.1_g000019
rosa_roxburghii	Rroxscaffold_6G00427090
rosa_rugosa	Rorug02G0617100 Rorug02G0617100
rosa_samantha	Rh3AG015400 Rh3BG015200 Rh3CG014100 Rh3DG015700
rosa_wichuraiana	Rw3G001140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1374
AccB1I	GGYRCC	1 cut(s)	3797
AccBSI	CCGCTC	3 cut(s)	769, 1712, 2356
AccI	GTMKAC	2 cut(s)	236, 2563
AccII	CGCG	1 cut(s)	3751
AciI	CCGC	7 cut(s)	769, 1591, 1710, 1893, 2356, 2718, 3837
AclI	AACGTT	1 cut(s)	1898
AcoI	YGGCCR	1 cut(s)	1527
AcsI	RAATTY	5 cut(s)	84, 658, 1286, 1931, 3465
AcuI	CTGAAG	5 cut(s)	186, 1178, 1320, 1631, 3642
AdeI	CACNNNGTG	1 cut(s)	642
AfaI	GTAC	9 cut(s)	308, 576, 711, 845, 959, 1368, 1543, 2722, 3305
AfeI	AGCGCT	2 cut(s)	365, 1401
AfiI	CCNNNNNNNGG	7 cut(s)	1590, 2805, 2945, 3130, 3131, 3407, 3408
AflII	CTTAAG	1 cut(s)	506
AflIII	ACRYGT	1 cut(s)	2790
AhlI	ACTAGT	1 cut(s)	3356
AjnI	CCWGG	5 cut(s)	79, 1774, 2831, 2880, 3238
AjuI	GAANNNNNNNTTGG	6 cut(s)	972, 1004, 2693, 2725, 3241, 3273
AleI	CACNNNNGTG	1 cut(s)	488
AloI	GAACNNNNNNTCC	4 cut(s)	1783, 1815, 3818, 3850
Alw21I	GWGCWC	5 cut(s)	1722, 2097, 2848, 3012, 3588
Alw26I	GTCTC	4 cut(s)	421, 1478, 2415, 3838
Alw44I	GTGCAC	1 cut(s)	1718
AlwNI	CAGNNNCTG	3 cut(s)	1735, 1889, 3290
Ama87I	CYCGRG	2 cut(s)	206, 422
Aor51HI	AGCGCT	2 cut(s)	365, 1401
AoxI	GGCC	6 cut(s)	419, 1527, 1691, 3457, 3685, 3864
ApaLI	GTGCAC	1 cut(s)	1718
ApoI	RAATTY	5 cut(s)	84, 658, 1286, 1931, 3465
ArsI	GACNNNNNNTTYG	2 cut(s)	69, 101
AseI	ATTAAT	1 cut(s)	435
Asp700I	GAANNNNTTC	4 cut(s)	699, 1233, 2600, 3024
AspLEI	GCGC	4 cut(s)	34, 366, 1402, 3751
AspS9I	GGNCC	8 cut(s)	77, 420, 440, 462, 1564, 1640, 2817, 3607
AsuHPI	GGTGA	2 cut(s)	996, 3533
AsuII	TTCGAA	1 cut(s)	1552
AsuNHI	GCTAGC	1 cut(s)	391
AvaI	CYCGRG	2 cut(s)	206, 422
AvaII	GGWCC	7 cut(s)	77, 440, 462, 1564, 1640, 2817, 3607
AxyI	CCTNAGG	1 cut(s)	1766
BaeGI	GKGCMC	2 cut(s)	1722, 3802
BalI	TGGCCA	1 cut(s)	1529
BamHI	GGATCC	2 cut(s)	1088, 1575
BanI	GGYRCC	1 cut(s)	3797
BanII	GRGCYC	4 cut(s)	1785, 3012, 3588, 3662
BauI	CACGAG	1 cut(s)	1950
BbsI	GAAGAC	3 cut(s)	174, 980, 1938
Bbv12I	GWGCWC	5 cut(s)	1722, 2097, 2848, 3012, 3588
BbvCI	CCTCAGC	2 cut(s)	267, 2130
BcgI	CGANNNNNNTGC	2 cut(s)	125, 159
BciT130I	CCWGG	5 cut(s)	81, 1776, 2833, 2882, 3240
BclI	TGATCA	1 cut(s)	499
BcoDI	GTCTC	4 cut(s)	421, 1478, 2415, 3838
BcuI	ACTAGT	1 cut(s)	3356
BfmI	CTRYAG	8 cut(s)	130, 396, 444, 1378, 3147, 3291, 3379, 3528
BfoI	RGCGCY	2 cut(s)	367, 1403
BfrI	CTTAAG	1 cut(s)	506
BglI	GCCNNNNNGGC	2 cut(s)	1028, 1780
BglII	AGATCT	4 cut(s)	913, 2416, 2488, 3295
BlpI	GCTNAGC	2 cut(s)	1853, 2076
BmcAI	AGTACT	1 cut(s)	711
Bme1390I	CCNGG	5 cut(s)	81, 1776, 2833, 2882, 3240
Bme18I	GGWCC	7 cut(s)	77, 440, 462, 1564, 1640, 2817, 3607
BmeT110I	CYCGRG	2 cut(s)	206, 422
BmgT120I	GGNCC	8 cut(s)	77, 420, 440, 462, 1564, 1640, 2817, 3607
BmiI	GGNNCC	9 cut(s)	1090, 1577, 1642, 2108, 2661, 2818, 2886, 2998, 3799
BmrFI	CCNGG	5 cut(s)	81, 1776, 2833, 2882, 3240
BmsI	GCATC	7 cut(s)	392, 1349, 1434, 1462, 2517, 2580, 3644
BmtI	GCTAGC	1 cut(s)	395
BpiI	GAAGAC	3 cut(s)	174, 980, 1938
BpmI	CTGGAG	3 cut(s)	71, 1797, 2467
Bpu10I	CCTNAGC	4 cut(s)	267, 632, 1713, 2130
Bpu1102I	GCTNAGC	2 cut(s)	1853, 2076
Bpu14I	TTCGAA	1 cut(s)	1552
BpuEI	CTTGAG	6 cut(s)	400, 2030, 2081, 2397, 2709, 3156
BsaAI	YACGTR	1 cut(s)	353
BsaBI	GATNNNNATC	2 cut(s)	918, 3441
BsaJI	CCNNGG	7 cut(s)	1497, 1688, 1985, 2831, 2880, 3125, 3238
BsaWI	WCCGGW	4 cut(s)	10, 104, 1754, 3810
BsaXI	ACNNNNNCTCC	2 cut(s)	3818, 3848
Bsc4I	CCNNNNNNNGG	7 cut(s)	1590, 2805, 2945, 3130, 3131, 3407, 3408
Bse118I	RCCGGY	1 cut(s)	2024
Bse1I	ACTGG	6 cut(s)	54, 893, 1613, 1694, 2550, 3372
Bse21I	CCTNAGG	1 cut(s)	1766
Bse3DI	GCAATG	3 cut(s)	24, 2825, 2912
Bse8I	GATNNNNATC	2 cut(s)	918, 3441
BseBI	CCWGG	5 cut(s)	81, 1776, 2833, 2882, 3240
BseDI	CCNNGG	7 cut(s)	1497, 1688, 1985, 2831, 2880, 3125, 3238
BseJI	GATNNNNATC	2 cut(s)	918, 3441
BseLI	CCNNNNNNNGG	7 cut(s)	1590, 2805, 2945, 3130, 3131, 3407, 3408
BseMI	GCAATG	3 cut(s)	24, 2825, 2912
BseMII	CTCAG	9 cut(s)	281, 660, 1006, 1727, 1794, 2090, 2121, 2343, 2588
BseNI	ACTGG	6 cut(s)	54, 893, 1613, 1694, 2550, 3372
BseRI	GAGGAG	2 cut(s)	620, 3494
BseSI	GKGCMC	2 cut(s)	1722, 3802
BseYI	CCCAGC	4 cut(s)	2199, 2634, 3064, 3517
BsgI	GTGCAG	4 cut(s)	1242, 1404, 3381, 3791
Bsh1236I	CGCG	1 cut(s)	3751
Bsh1285I	CGRYCG	1 cut(s)	1971
BshFI	GGCC	6 cut(s)	421, 1529, 1693, 3459, 3687, 3866
BshNI	GGYRCC	1 cut(s)	3797
BsiEI	CGRYCG	1 cut(s)	1971
BsiHKAI	GWGCWC	5 cut(s)	1722, 2097, 2848, 3012, 3588
BsiHKCI	CYCGRG	2 cut(s)	206, 422
BsiSI	CCGG	6 cut(s)	11, 105, 1755, 2025, 3254, 3811
BslFI	GGGAC	1 cut(s)	1281
BslI	CCNNNNNNNGG	7 cut(s)	1590, 2805, 2945, 3130, 3131, 3407, 3408
BsmAI	GTCTC	4 cut(s)	421, 1478, 2415, 3838
BsmBI	CGTCTC	2 cut(s)	421, 3838
BsmFI	GGGAC	1 cut(s)	1281
BsmI	GAATGC	4 cut(s)	199, 517, 1979, 2787
BsnI	GGCC	6 cut(s)	421, 1529, 1693, 3459, 3687, 3866
BsoBI	CYCGRG	2 cut(s)	206, 422
Bsp119I	TTCGAA	1 cut(s)	1552
Bsp1286I	GDGCHC	8 cut(s)	1722, 1785, 2097, 2848, 3012, 3588, 3662, 3802
Bsp1407I	TGTACA	3 cut(s)	574, 843, 3303
Bsp1720I	GCTNAGC	2 cut(s)	1853, 2076
BspACI	CCGC	7 cut(s)	769, 1591, 1710, 1893, 2356, 2718, 3837
BspANI	GGCC	6 cut(s)	421, 1529, 1693, 3459, 3687, 3866
BspCNI	CTCAG	9 cut(s)	280, 661, 1005, 1726, 1795, 2089, 2122, 2344, 2587
BspFNI	CGCG	1 cut(s)	3751
BspHI	TCATGA	2 cut(s)	939, 1009
BspLI	GGNNCC	9 cut(s)	1090, 1577, 1642, 2108, 2661, 2818, 2886, 2998, 3799
BspMAI	CTGCAG	3 cut(s)	400, 3151, 3295
BspOI	GCTAGC	1 cut(s)	395
BspT104I	TTCGAA	1 cut(s)	1552
BspT107I	GGYRCC	1 cut(s)	3797
BspTI	CTTAAG	1 cut(s)	506
BsrBI	CCGCTC	3 cut(s)	769, 1712, 2356
BsrDI	GCAATG	3 cut(s)	24, 2825, 2912
BsrFI	RCCGGY	1 cut(s)	2024
BsrGI	TGTACA	3 cut(s)	574, 843, 3303
BsrI	ACTGG	6 cut(s)	54, 893, 1613, 1694, 2550, 3372
BssAI	RCCGGY	1 cut(s)	2024
BssECI	CCNNGG	7 cut(s)	1497, 1688, 1985, 2831, 2880, 3125, 3238
BssNAI	GTATAC	1 cut(s)	237
BssSI	CACGAG	1 cut(s)	1950
BssT1I	CCWWGG	3 cut(s)	1688, 1985, 3125
Bst1107I	GTATAC	1 cut(s)	237
Bst2BI	CACGAG	1 cut(s)	1950
Bst2UI	CCWGG	5 cut(s)	81, 1776, 2833, 2882, 3240
Bst4CI	ACNGT	9 cut(s)	119, 448, 579, 1830, 2106, 2768, 3380, 3455, 3832
Bst6I	CTCTTC	1 cut(s)	1986
BstAFI	CTTAAG	1 cut(s)	506
BstAPI	GCANNNNNTGC	4 cut(s)	1172, 1726, 3290, 3574
BstAUI	TGTACA	3 cut(s)	574, 843, 3303
BstBAI	YACGTR	1 cut(s)	353
BstBI	TTCGAA	1 cut(s)	1552
BstEII	GGTNACC	1 cut(s)	1760
BstFNI	CGCG	1 cut(s)	3751
BstH2I	RGCGCY	2 cut(s)	367, 1403
BstHHI	GCGC	4 cut(s)	34, 366, 1402, 3751
BstMAI	GTCTC	4 cut(s)	421, 1478, 2415, 3838
BstMCI	CGRYCG	1 cut(s)	1971
BstNI	CCWGG	5 cut(s)	81, 1776, 2833, 2882, 3240
BstNSI	RCATGY	4 cut(s)	668, 1496, 2468, 2794
BstPI	GGTNACC	1 cut(s)	1760
BstSCI	CCNGG	5 cut(s)	79, 1774, 2831, 2880, 3238
BstSFI	CTRYAG	8 cut(s)	130, 396, 444, 1378, 3147, 3291, 3379, 3528
BstSLI	GKGCMC	2 cut(s)	1722, 3802
BstUI	CGCG	1 cut(s)	3751
BstV2I	GAAGAC	3 cut(s)	174, 980, 1938
BstXI	CCANNNNNNTGG	1 cut(s)	87
BstZ17I	GTATAC	1 cut(s)	237
Bsu36I	CCTNAGG	1 cut(s)	1766
BsuRI	GGCC	6 cut(s)	421, 1529, 1693, 3459, 3687, 3866
BtgZI	GCGATG	1 cut(s)	150
BtsI	GCAGTG	3 cut(s)	1720, 2288, 3848
BtsIMutI	CAGTG	9 cut(s)	47, 584, 1720, 2288, 2543, 2773, 3365, 3460, 3848
CaiI	CAGNNNCTG	3 cut(s)	1735, 1889, 3290
CciI	TCATGA	2 cut(s)	939, 1009
CfoI	GCGC	4 cut(s)	34, 366, 1402, 3751
Cfr10I	RCCGGY	1 cut(s)	2024
Cfr13I	GGNCC	8 cut(s)	77, 420, 440, 462, 1564, 1640, 2817, 3607
CseI	GACGC	1 cut(s)	438
Csp6I	GTAC	9 cut(s)	307, 575, 710, 844, 958, 1367, 1542, 2721, 3304
CspCI	CAANNNNNGTGG	2 cut(s)	3775, 3810
CviQI	GTAC	9 cut(s)	307, 575, 710, 844, 958, 1367, 1542, 2721, 3304
DraI	TTTAAA	2 cut(s)	2733, 3208
DraIII	CACNNNGTG	1 cut(s)	642
EaeI	YGGCCR	1 cut(s)	1527
Eam1104I	CTCTTC	1 cut(s)	1986
EarI	CTCTTC	1 cut(s)	1986
Ecl136II	GAGCTC	2 cut(s)	3010, 3586
Eco130I	CCWWGG	3 cut(s)	1688, 1985, 3125
Eco147I	AGGCCT	1 cut(s)	3866
Eco24I	GRGCYC	4 cut(s)	1785, 3012, 3588, 3662
Eco32I	GATATC	3 cut(s)	2455, 3079, 3421
Eco47I	GGWCC	7 cut(s)	77, 440, 462, 1564, 1640, 2817, 3607
Eco47III	AGCGCT	2 cut(s)	365, 1401
Eco53kI	GAGCTC	2 cut(s)	3010, 3586
Eco57I	CTGAAG	5 cut(s)	186, 1178, 1320, 1631, 3642
Eco81I	CCTNAGG	1 cut(s)	1766
Eco88I	CYCGRG	2 cut(s)	206, 422
Eco91I	GGTNACC	1 cut(s)	1760
EcoICRI	GAGCTC	2 cut(s)	3010, 3586
EcoO109I	RGGNCCY	2 cut(s)	1564, 3607
EcoO65I	GGTNACC	1 cut(s)	1760
EcoRII	CCWGG	5 cut(s)	79, 1774, 2831, 2880, 3238
EcoRV	GATATC	3 cut(s)	2455, 3079, 3421
EcoT14I	CCWWGG	3 cut(s)	1688, 1985, 3125
EcoT38I	GRGCYC	4 cut(s)	1785, 3012, 3588, 3662
ErhI	CCWWGG	3 cut(s)	1688, 1985, 3125
Esp3I	CGTCTC	2 cut(s)	421, 3838
FalI	AAGNNNNNCTT	4 cut(s)	687, 719, 1841, 1873
FaqI	GGGAC	1 cut(s)	1281
FbaI	TGATCA	1 cut(s)	499
FblI	GTMKAC	2 cut(s)	236, 2563
FriOI	GRGCYC	4 cut(s)	1785, 3012, 3588, 3662
GlaI	GCGC	4 cut(s)	33, 365, 1401, 3750
GsaI	CCCAGC	4 cut(s)	2203, 2638, 3068, 3521
GsuI	CTGGAG	3 cut(s)	71, 1797, 2467
HaeII	RGCGCY	2 cut(s)	367, 1403
HaeIII	GGCC	6 cut(s)	421, 1529, 1693, 3459, 3687, 3866
HapII	CCGG	6 cut(s)	11, 105, 1755, 2025, 3254, 3811
HgaI	GACGC	1 cut(s)	438
HhaI	GCGC	4 cut(s)	34, 366, 1402, 3751
Hin6I	GCGC	4 cut(s)	32, 364, 1400, 3749
HinP1I	GCGC	4 cut(s)	32, 364, 1400, 3749
HincII	GTYRAC	2 cut(s)	1902, 3199
HindII	GTYRAC	2 cut(s)	1902, 3199
HindIII	AAGCTT	2 cut(s)	372, 1850
HpaII	CCGG	6 cut(s)	11, 105, 1755, 2025, 3254, 3811
HphI	GGTGA	2 cut(s)	996, 3533
Hpy166II	GTNNAC	8 cut(s)	237, 575, 685, 1720, 1902, 2283, 2564, 3199
Hpy8I	GTNNAC	8 cut(s)	237, 575, 685, 1720, 1902, 2283, 2564, 3199
HpyAV	CCTTC	8 cut(s)	1007, 1223, 1326, 1555, 1655, 2316, 3271, 3470
HpyCH4III	ACNGT	9 cut(s)	119, 448, 579, 1830, 2106, 2768, 3380, 3455, 3832
HpyCH4IV	ACGT	4 cut(s)	352, 690, 1369, 1898
HpySE526I	ACGT	4 cut(s)	352, 690, 1369, 1898
HspAI	GCGC	4 cut(s)	32, 364, 1400, 3749
Ksp22I	TGATCA	1 cut(s)	499
LmnI	GCTCC	2 cut(s)	107, 2940
LweI	GCATC	7 cut(s)	392, 1349, 1434, 1462, 2517, 2580, 3644
MaeII	ACGT	4 cut(s)	352, 690, 1369, 1898
MaeIII	GTNAC	7 cut(s)	448, 474, 732, 1454, 1760, 2013, 2953
MbiI	CCGCTC	3 cut(s)	769, 1712, 2356
MfeI	CAATTG	1 cut(s)	123
MhlI	GDGCHC	8 cut(s)	1722, 1785, 2097, 2848, 3012, 3588, 3662, 3802
MlsI	TGGCCA	1 cut(s)	1529
MluNI	TGGCCA	1 cut(s)	1529
MlyI	GAGTC	9 cut(s)	352, 740, 845, 1588, 1952, 2021, 2417, 2495, 3491
MmeI	TCCRAC	1 cut(s)	591
Mox20I	TGGCCA	1 cut(s)	1529
MroXI	GAANNNNTTC	4 cut(s)	699, 1233, 2600, 3024
MscI	TGGCCA	1 cut(s)	1529
MslI	CAYNNNNRTG	3 cut(s)	488, 1656, 2469
Msp20I	TGGCCA	1 cut(s)	1529
MspA1I	CMGCKG	1 cut(s)	3574
MspCI	CTTAAG	1 cut(s)	506
MspI	CCGG	6 cut(s)	11, 105, 1755, 2025, 3254, 3811
MspR9I	CCNGG	5 cut(s)	81, 1776, 2833, 2882, 3240
MunI	CAATTG	1 cut(s)	123
Mva1269I	GAATGC	4 cut(s)	199, 517, 1979, 2787
MvaI	CCWGG	5 cut(s)	81, 1776, 2833, 2882, 3240
MvnI	CGCG	1 cut(s)	3751
NheI	GCTAGC	1 cut(s)	391
NlaIV	GGNNCC	9 cut(s)	1090, 1577, 1642, 2108, 2661, 2818, 2886, 2998, 3799
NmeAIII	GCCGAG	1 cut(s)	1522
NmuCI	GTSAC	4 cut(s)	732, 1454, 2013, 2953
NspI	RCATGY	4 cut(s)	668, 1496, 2468, 2794
NspV	TTCGAA	1 cut(s)	1552
OliI	CACNNNNGTG	1 cut(s)	488
PaeI	GCATGC	2 cut(s)	668, 2468
PaeR7I	CTCGAG	1 cut(s)	206
PagI	TCATGA	2 cut(s)	939, 1009
PceI	AGGCCT	1 cut(s)	3866
PciI	ACATGT	1 cut(s)	2790
PctI	GAATGC	4 cut(s)	199, 517, 1979, 2787
PdmI	GAANNNNTTC	4 cut(s)	699, 1233, 2600, 3024
Ple19I	CGATCG	1 cut(s)	1971
PleI	GAGTC	9 cut(s)	351, 739, 844, 1588, 1951, 2020, 2416, 2495, 3490
PpsI	GAGTC	9 cut(s)	351, 739, 844, 1588, 1951, 2020, 2416, 2495, 3490
Ppu21I	YACGTR	1 cut(s)	353
PpuMI	RGGWCCY	2 cut(s)	1564, 3607
PscI	ACATGT	1 cut(s)	2790
PshBI	ATTAAT	1 cut(s)	435
PsiI	TTATAA	1 cut(s)	1374
Psp124BI	GAGCTC	2 cut(s)	3012, 3588
Psp1406I	AACGTT	1 cut(s)	1898
Psp5II	RGGWCCY	2 cut(s)	1564, 3607
Psp6I	CCWGG	5 cut(s)	79, 1774, 2831, 2880, 3238
PspEI	GGTNACC	1 cut(s)	1760
PspFI	CCCAGC	4 cut(s)	2199, 2634, 3064, 3517
PspGI	CCWGG	5 cut(s)	79, 1774, 2831, 2880, 3238
PspN4I	GGNNCC	9 cut(s)	1090, 1577, 1642, 2108, 2661, 2818, 2886, 2998, 3799
PspPI	GGNCC	8 cut(s)	77, 420, 440, 462, 1564, 1640, 2817, 3607
PspPPI	RGGWCCY	2 cut(s)	1564, 3607
PspXI	VCTCGAGB	1 cut(s)	206
PstI	CTGCAG	3 cut(s)	400, 3151, 3295
PstNI	CAGNNNCTG	3 cut(s)	1735, 1889, 3290
PvuI	CGATCG	1 cut(s)	1971
PvuII	CAGCTG	1 cut(s)	3574
RsaI	GTAC	9 cut(s)	308, 576, 711, 845, 959, 1368, 1543, 2722, 3305
RsaNI	GTAC	9 cut(s)	307, 575, 710, 844, 958, 1367, 1542, 2721, 3304
RseI	CAYNNNNRTG	3 cut(s)	488, 1656, 2469
SacI	GAGCTC	2 cut(s)	3012, 3588
Sau96I	GGNCC	8 cut(s)	77, 420, 440, 462, 1564, 1640, 2817, 3607
ScaI	AGTACT	1 cut(s)	711
SchI	GAGTC	9 cut(s)	352, 740, 845, 1588, 1952, 2021, 2417, 2495, 3491
ScrFI	CCNGG	5 cut(s)	81, 1776, 2833, 2882, 3240
SduI	GDGCHC	8 cut(s)	1722, 1785, 2097, 2848, 3012, 3588, 3662, 3802
SfaNI	GCATC	7 cut(s)	392, 1349, 1434, 1462, 2517, 2580, 3644
SfcI	CTRYAG	8 cut(s)	130, 396, 444, 1378, 3147, 3291, 3379, 3528
Sfr274I	CTCGAG	1 cut(s)	206
SfuI	TTCGAA	1 cut(s)	1552
SinI	GGWCC	7 cut(s)	77, 440, 462, 1564, 1640, 2817, 3607
SlaI	CTCGAG	1 cut(s)	206
SmiI	ATTTAAAT	1 cut(s)	2733
SmiMI	CAYNNNNRTG	3 cut(s)	488, 1656, 2469
SmlI	CTYRAG	8 cut(s)	206, 415, 506, 2009, 2096, 2412, 2724, 3171
SmoI	CTYRAG	8 cut(s)	206, 415, 506, 2009, 2096, 2412, 2724, 3171
SpeI	ACTAGT	1 cut(s)	3356
SphI	GCATGC	2 cut(s)	668, 2468
SseBI	AGGCCT	1 cut(s)	3866
SsiI	CCGC	7 cut(s)	769, 1591, 1710, 1893, 2356, 2718, 3837
SspI	AATATT	3 cut(s)	2383, 3162, 3341
SstI	GAGCTC	2 cut(s)	3012, 3588
StuI	AGGCCT	1 cut(s)	3866
StyD4I	CCNGG	5 cut(s)	79, 1774, 2831, 2880, 3238
StyI	CCWWGG	3 cut(s)	1688, 1985, 3125
SwaI	ATTTAAAT	1 cut(s)	2733
TaaI	ACNGT	9 cut(s)	119, 448, 579, 1830, 2106, 2768, 3380, 3455, 3832
TaiI	ACGT	4 cut(s)	355, 693, 1372, 1901
TatI	WGTACW	5 cut(s)	574, 709, 843, 957, 3303
TauI	GCSGC	3 cut(s)	772, 1712, 1895
TscAI	CASTG	9 cut(s)	54, 584, 1727, 2295, 2550, 2773, 3372, 3460, 3855
TseFI	GTSAC	4 cut(s)	732, 1454, 2013, 2953
Tsp45I	GTSAC	4 cut(s)	732, 1454, 2013, 2953
TspGWI	ACGGA	3 cut(s)	668, 1085, 1919
TspRI	CASTG	9 cut(s)	54, 584, 1727, 2295, 2550, 2773, 3372, 3460, 3855
Vha464I	CTTAAG	1 cut(s)	506
VneI	GTGCAC	1 cut(s)	1718
VpaK11BI	GGWCC	7 cut(s)	77, 440, 462, 1564, 1640, 2817, 3607
VspI	ATTAAT	1 cut(s)	435
XapI	RAATTY	5 cut(s)	84, 658, 1286, 1931, 3465
XbaI	TCTAGA	3 cut(s)	2032, 2851, 3475
XceI	RCATGY	4 cut(s)	668, 1496, 2468, 2794
XhoI	CTCGAG	1 cut(s)	206
XmiI	GTMKAC	2 cut(s)	236, 2563
XmnI	GAANNNNTTC	4 cut(s)	699, 1233, 2600, 3024
ZrmI	AGTACT	1 cut(s)	711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.