RLG00000026226

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015063
Physical Location & Seq
Forward (+)
1385433 .. 1387086
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026226

Sequence Viewer

Length: 825 bp
ATGAAACACCCTCACCTTCTCTATTTCCTCTCACTCTCCCTCCCACTGCAATCTTCATCCCCGTCGCTCAAAAACCCTAACCCCCTTTTCTTCTCGTCAGAGTCGGCCGTCGGAACAGCTTACGTCATCGCCGGTACCTCTGGGGGCATGGCTTGTATACGAAATGGCAGAAATGCCCTCCGTCAGATCATCAGAGACACTCACAACGTCGTCCATTCTTCCGAGTCGGAGCGCATGATGAATCCTCTGGTTTTCGCTTGCCAAGGAGTTCGGCATAGGAAATTGGAAGTGATTTTGACGACGAATGTGGATAAGCTCGGCAAGGCCGGTGAGACTGTGAAGGTTGCGCCGGGCCATTTCCGCAACCACTTGATGCCGAAATTGCTTGCTGTACCCAACATTGACAAGTATGCCTATCTCATAAGACAGCAGCGAAAGAATGCTCTGCTGGAAGAAGAAGAGGAGAAGGAGGAGGTTAAAGTGGTTACTGTGTCCGAGGAAGTTAAGATGAAAGAATATGAAAAGGCAGCGAAGCGTCTGGATAATGCTCGTCTGGTATTGCGGAGGAACATTAATCTTGAAAAGTTCAGGACACGTGCAACAAAGGAAGACCCTATAGAATTGCGTTCTACTGTGACCAAGGAGGATATTGTGGCTGAGGTGGCAAGGCAGCTTTCTATAGGCTTAGAACCTGAGAATGTGCATCTACCATCTCCATTGTCAACTGTAGGAGAGTATGAGGTACCACTGCGCCTTCCGAGATCCATCCCTTTGCCGGCAGGGAAGGTTCATTGGACACTAAATGTTAAAATCCGATGCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L9_C Ribosomal_L9_N

Protein Analysis

275

Amino Acids

30.86

Weight (kDa)

9.53

Isoelectric Point (pI)

56.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L9_N PF01281 96 - 132 8.8e-13 Ribosomal protein L9, N-terminal domain
Ribosomal_L9_C PF03948 207 - 255 3.8e-06 Ribosomal protein L9, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015063)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53070
fragaria_vesca FvH4_7g32000
malus_domestica MD07G1293600.v1.1
prunus_persica Prupe.2G313400_v2.0.a1 Prupe.2G313400_v2.0.a1 Prupe.2G313400_v2.0.a1
pyrus_communis pycom07g26640
rosa_chinensis RchiOBHm_Chr1g0381511
rosa_laevigata RLG00000026226
rosa_roxburghii Rroxscaffold_4G00278600
rosa_rugosa Rorug01G0429100
rosa_samantha Rh1AG452300 Rh1BG409600 Rh1CG424100 Rh1DG440900
rosa_wichuraiana Rw1G039490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 820
Acc65I GGTACC 2 cut(s) 134, 742
AccB1I GGYRCC 2 cut(s) 134, 742
AccI GTMKAC 1 cut(s) 157
AciI CCGC 2 cut(s) 361, 562
AclWI GGATC 1 cut(s) 756
AcoI YGGCCR 1 cut(s) 105
AcvI CACGTG 1 cut(s) 596
AfaI GTAC 3 cut(s) 136, 393, 744
AfiI CCNNNNNNNGG 1 cut(s) 775
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 1 cut(s) 581
AluBI AGCT 3 cut(s) 119, 316, 673
AluI AGCT 3 cut(s) 119, 316, 673
Alw26I GTCTC 2 cut(s) 189, 326
AlwI GGATC 1 cut(s) 756
AoxI GGCC 3 cut(s) 105, 324, 352
ApeKI GCWGC 3 cut(s) 430, 527, 670
AseI ATTAAT 1 cut(s) 573
Asp718I GGTACC 2 cut(s) 134, 742
AspLEI GCGC 4 cut(s) 234, 349, 753, 821
AspS9I GGNCC 1 cut(s) 352
AsuC2I CCSGG 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 5, 341
BanI GGYRCC 2 cut(s) 134, 742
BbrPI CACGTG 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 615
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 3 cut(s) 442, 539, 682
BccI CCATC 2 cut(s) 718, 773
BceAI ACGGC 1 cut(s) 92
BcnI CCSGG 1 cut(s) 351
BcoDI GTCTC 2 cut(s) 189, 326
BfmI CTRYAG 3 cut(s) 615, 678, 726
BisI GCNGC 3 cut(s) 431, 528, 671
BlsI GCNGC 3 cut(s) 432, 529, 672
Bme1390I CCNGG 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 136, 744
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 3 cut(s) 363, 712, 806
BpiI GAAGAC 1 cut(s) 615
Bpu10I CCTNAGC 1 cut(s) 657
BpuMI CCSGG 1 cut(s) 351
BsaAI YACGTR 1 cut(s) 596
BsaJI CCNNGG 3 cut(s) 262, 495, 639
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 775
Bse118I RCCGGY 3 cut(s) 131, 326, 775
BseDI CCNNGG 3 cut(s) 262, 495, 639
BseGI GGATG 2 cut(s) 56, 765
BseLI CCNNNNNNNGG 1 cut(s) 775
BseMII CTCAG 2 cut(s) 648, 684
BseRI GAGGAG 2 cut(s) 476, 485
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 3 cut(s) 442, 539, 682
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 107, 326, 354
BshNI GGYRCC 2 cut(s) 134, 742
BsiEI CGRYCG 1 cut(s) 108
BsiSI CCGG 4 cut(s) 132, 327, 350, 776
BslI CCNNNNNNNGG 1 cut(s) 775
BsmAI GTCTC 2 cut(s) 189, 326
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 3 cut(s) 107, 326, 354
Bsp143I GATC 2 cut(s) 186, 761
BspACI CCGC 2 cut(s) 361, 562
BspANI GGCC 3 cut(s) 107, 326, 354
BspCNI CTCAG 2 cut(s) 649, 685
BspLI GGNNCC 2 cut(s) 136, 744
BspPI GGATC 1 cut(s) 756
BspT107I GGYRCC 2 cut(s) 134, 742
BsrFI RCCGGY 3 cut(s) 131, 326, 775
BssAI RCCGGY 3 cut(s) 131, 326, 775
BssECI CCNNGG 3 cut(s) 262, 495, 639
BssMI GATC 2 cut(s) 186, 761
BssNAI GTATAC 1 cut(s) 158
BssT1I CCWWGG 2 cut(s) 262, 639
Bst1107I GTATAC 1 cut(s) 158
Bst4CI ACNGT 4 cut(s) 337, 490, 634, 727
Bst6I CTCTTC 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 596
BstC8I GCNNGC 3 cut(s) 259, 387, 777
BstDEI CTNAG 3 cut(s) 657, 685, 693
BstF5I GGATG 2 cut(s) 56, 765
BstHHI GCGC 4 cut(s) 234, 349, 753, 821
BstKTI GATC 2 cut(s) 189, 764
BstMAI GTCTC 2 cut(s) 189, 326
BstMBI GATC 2 cut(s) 186, 761
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 3 cut(s) 360, 382, 662
BstSCI CCNGG 1 cut(s) 349
BstSFI CTRYAG 3 cut(s) 615, 678, 726
BstV1I GCAGC 3 cut(s) 442, 539, 682
BstV2I GAAGAC 1 cut(s) 615
BstX2I RGATCY 1 cut(s) 761
BstYI RGATCY 1 cut(s) 761
BstZ17I GTATAC 1 cut(s) 158
BstZI CGGCCG 1 cut(s) 105
BsuRI GGCC 3 cut(s) 107, 326, 354
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 2 cut(s) 56, 765
BtsI GCAGTG 2 cut(s) 44, 746
BtsIMutI CAGTG 2 cut(s) 44, 746
Cac8I GCNNGC 3 cut(s) 259, 387, 777
CfoI GCGC 4 cut(s) 234, 349, 753, 821
Cfr10I RCCGGY 3 cut(s) 131, 326, 775
Cfr13I GGNCC 1 cut(s) 352
CseI GACGC 1 cut(s) 524
Csp6I GTAC 3 cut(s) 135, 392, 743
CviAII CATG 2 cut(s) 148, 235
CviJI RGCY 9 cut(s) 107, 119, 152, 316, 326, 354, 656, 673, 684
CviKI_1 RGCY 9 cut(s) 107, 119, 152, 316, 326, 354, 656, 673, 684
CviQI GTAC 3 cut(s) 135, 392, 743
DdeI CTNAG 3 cut(s) 657, 685, 693
DpnI GATC 2 cut(s) 188, 763
DpnII GATC 2 cut(s) 186, 761
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 262, 639
Eco52I CGGCCG 1 cut(s) 105
Eco72I CACGTG 1 cut(s) 596
EcoT14I CCWWGG 2 cut(s) 262, 639
ErhI CCWWGG 2 cut(s) 262, 639
FaeI CATG 2 cut(s) 151, 238
FatI CATG 2 cut(s) 147, 234
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 3 cut(s) 431, 528, 671
FokI GGATG 2 cut(s) 43, 752
Fsp4HI GCNGC 3 cut(s) 431, 528, 671
FspAI RTGCGCAY 1 cut(s) 820
FspI TGCGCA 1 cut(s) 820
GlaI GCGC 4 cut(s) 233, 348, 752, 820
GluI GCNGC 3 cut(s) 431, 528, 671
HaeIII GGCC 3 cut(s) 107, 326, 354
HapII CCGG 4 cut(s) 132, 327, 350, 776
HgaI GACGC 1 cut(s) 524
HhaI GCGC 4 cut(s) 234, 349, 753, 821
Hin1II CATG 2 cut(s) 151, 238
Hin6I GCGC 4 cut(s) 232, 347, 751, 819
HinP1I GCGC 4 cut(s) 232, 347, 751, 819
HincII GTYRAC 1 cut(s) 723
HindII GTYRAC 1 cut(s) 723
HinfI GANTC 3 cut(s) 101, 224, 241
HpaII CCGG 4 cut(s) 132, 327, 350, 776
HphI GGTGA 2 cut(s) 5, 341
Hpy166II GTNNAC 2 cut(s) 158, 723
Hpy188I TCNGA 9 cut(s) 100, 113, 186, 194, 223, 229, 496, 759, 815
Hpy188III TCNNGA 3 cut(s) 539, 578, 589
Hpy8I GTNNAC 2 cut(s) 158, 723
Hpy99I CGWCG 4 cut(s) 67, 113, 212, 304
HpyAV CCTTC 5 cut(s) 26, 334, 460, 764, 778
HpyCH4III ACNGT 4 cut(s) 337, 490, 634, 727
HpyCH4IV ACGT 3 cut(s) 123, 207, 595
HpyCH4V TGCA 3 cut(s) 49, 599, 703
HpyF10VI GCNNNNNNNGC 3 cut(s) 360, 382, 662
HpyF3I CTNAG 3 cut(s) 657, 685, 693
HpySE526I ACGT 3 cut(s) 123, 207, 595
Hsp92II CATG 2 cut(s) 151, 238
HspAI GCGC 4 cut(s) 232, 347, 751, 819
KpnI GGTACC 2 cut(s) 138, 746
KroI GCCGGC 1 cut(s) 775
KroNI GCCGGC 1 cut(s) 777
Kzo9I GATC 2 cut(s) 186, 761
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 3 cut(s) 442, 539, 682
LweI GCATC 3 cut(s) 363, 712, 806
MaeII ACGT 3 cut(s) 123, 207, 595
MaeIII GTNAC 2 cut(s) 484, 634
MalI GATC 2 cut(s) 188, 763
MboI GATC 2 cut(s) 186, 761
MboII GAAGA 7 cut(s) 45, 82, 210, 464, 467, 470, 620
MflI RGATCY 1 cut(s) 761
MluCI AATT 3 cut(s) 281, 380, 620
MlyI GAGTC 2 cut(s) 110, 233
MmeI TCCRAC 2 cut(s) 91, 207
MroNI GCCGGC 1 cut(s) 775
MseI TTAA 4 cut(s) 477, 504, 573, 807
MspI CCGG 4 cut(s) 132, 327, 350, 776
MspR9I CCNGG 1 cut(s) 351
Mva1269I GAATGC 1 cut(s) 445
MwoI GCNNNNNNNGC 3 cut(s) 360, 382, 662
NaeI GCCGGC 1 cut(s) 777
NciI CCSGG 1 cut(s) 351
NdeII GATC 2 cut(s) 186, 761
NgoMIV GCCGGC 1 cut(s) 775
NlaIII CATG 2 cut(s) 151, 238
NlaIV GGNNCC 2 cut(s) 136, 744
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 634
NsbI TGCGCA 1 cut(s) 820
PctI GAATGC 1 cut(s) 445
PdiI GCCGGC 1 cut(s) 777
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 3 cut(s) 432, 529, 672
PleI GAGTC 2 cut(s) 109, 232
PmaCI CACGTG 1 cut(s) 596
PmlI CACGTG 1 cut(s) 596
PpsI GAGTC 2 cut(s) 109, 232
Ppu21I YACGTR 1 cut(s) 596
PshBI ATTAAT 1 cut(s) 573
PspCI CACGTG 1 cut(s) 596
PspN4I GGNNCC 2 cut(s) 136, 744
PspPI GGNCC 1 cut(s) 352
PsuI RGATCY 1 cut(s) 761
RsaI GTAC 3 cut(s) 136, 393, 744
RsaNI GTAC 3 cut(s) 135, 392, 743
SaqAI TTAA 4 cut(s) 477, 504, 573, 807
SatI GCNGC 3 cut(s) 431, 528, 671
Sau3AI GATC 2 cut(s) 186, 761
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 2 cut(s) 110, 233
ScrFI CCNGG 1 cut(s) 351
SfaNI GCATC 3 cut(s) 363, 712, 806
SfcI CTRYAG 3 cut(s) 615, 678, 726
Sse9I AATT 3 cut(s) 281, 380, 620
SsiI CCGC 2 cut(s) 361, 562
StyD4I CCNGG 1 cut(s) 349
StyI CCWWGG 2 cut(s) 262, 639
TaaI ACNGT 4 cut(s) 337, 490, 634, 727
TaiI ACGT 3 cut(s) 126, 210, 598
TasI AATT 3 cut(s) 281, 380, 620
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 4 cut(s) 477, 504, 573, 807
Tru9I TTAA 4 cut(s) 477, 504, 573, 807
TscAI CASTG 2 cut(s) 51, 753
TseFI GTSAC 1 cut(s) 634
TseI GCWGC 3 cut(s) 430, 527, 670
Tsp45I GTSAC 1 cut(s) 634
TspDTI ATGAA 6 cut(s) 17, 45, 254, 524, 534, 779
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 51, 753
VspI ATTAAT 1 cut(s) 573
XmiI GTMKAC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.