RLG00000027446

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018419
Physical Location & Seq
Reverse (-)
11729265 .. 11731361
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027446

Sequence Viewer

Length: 1278 bp
ATGGGAAGAAGACGATCGAAATCCTCTTCGAAAAGGAAGAAAAAGAAAGAGAAGACATACCCGTTGGACAAAATAAGCAACTTACCAAGTGATGTTATACAGCATATTTTGTCATATTTGCACTTCAAGGAGGCAGTGAAAACAAGTGTGTTATCGAAAGCATGGAGACACAAATGGGCTATGCTTCCCTCCCTTGCCTTTGATGATACCGATGGCAGCTCCATTGAGAGAATTGTCAATCATGTTCTCTTTTCTCACACTGGTCCTGTAGACGCTTTCAAGCTTTCTACTGCAAGAAATCTAGACACTAGAGATATTGATCTATGGATTTCTCGTCTATCGAGATACTCTATCAAGGAGTTTATACTGAAATTATGGAATAGGTTTTTCAAATACGATGTCTTTTCCCGTTTGTTTTCTTTTCAAGATTTGACTCGCTTGGAGTTATGTAATTGTTCCCTGAAACCTCCATCCACATTCGAAGGCTTCCGGGTGTTGAAGGGCCTTCATATTCGAAGAGTTACAGTGGCTCAAGATGCGTTGGAAAAACTGATCGCGAGTGGTCCTCTTCTTGAGAGAATGACTCTATATGACATAACGCGTCTCACTCAGCTCAACATTTATGCACAGAATCTCCAATTCCTTAAAGTCGGAGGTGTTTTTCAGGATTTGAGACTTAAGAATACCACAAATCTCGTTGATGTCTCAATTGATGGAAAATGGGACTCCAGTGAATTCTTCAAGTTTTTTGTTCAGCTGCCTATTATTGAAAGGCTCACAATTAAGTTTAAAAGGAGCAGTAGGATGAGGTTAGATCAGCCCAACTGGAATTGCACATTCACCCAACTTCAAGTTTTGAAAGTAACTGGCTATTTTGGCAAACACACAGAGGAAGTAGAGTTCATCAGATCTGTGCTTTCAAGTTCACCTGTGCTCAAGAGGATTGCTCTTCAACCTGTTTCTGAGAACTATCGGCGTCACCTTCCTTCGGTAAGCGGCGGCGACAAACTTCATTGCTGGCTTCTCTCTTTGGATAGCGACGACAACACATCTATTCGTCTGGCTCTCTCTTCGGTTAGAGAAGACGTTTTTGCTGTAATTGGTGGAGATCAATTTCTGCTTGACCGGATCTGGATTGGTGGACGTACTCCAATTGGTTGGGCTGGTGGATCTGCGCTTGATTTGACGAACTATGGTGATGACTGCTACGCATGGCGCGGTGTTCTGAATCACAAAACTCTCGGAGGCGGTGCTTATGTGGAGAGCGGGGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

426

Amino Acids

48.74

Weight (kDa)

9.61

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 25 - 63 7.9e-09 F-box domain
LRR_At1g61320_AtMIF1 PF23622 90 - 220 7.6e-12 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 107 - 235 3.6e-11 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018419)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17800
rosa_chinensis RchiOBHm_Chr1g0365081
rosa_laevigata RLG00000027446
rosa_multiflora Rmu_sc0022857.1_g000001 Rmu_sc0034521.1_g000001
rosa_roxburghii Rroxscaffold_4G00291490 Rroxscaffold_4G00291940
rosa_rugosa Rorug01G0319400
rosa_samantha Rh1AG328400 Rh1DG321400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1266
AccI GTMKAC 1 cut(s) 270
AccII CGCG 3 cut(s) 557, 601, 1218
AciI CCGC 5 cut(s) 996, 999, 1218, 1248, 1266
AclWI GGATC 2 cut(s) 1136, 1177
AcsI RAATTY 1 cut(s) 734
AcyI GRCGYC 1 cut(s) 976
AfaI GTAC 1 cut(s) 1147
AfiI CCNNNNNNNGG 1 cut(s) 988
AflII CTTAAG 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 599
AloI GAACNNNNNNTCC 2 cut(s) 884, 916
AluBI AGCT 4 cut(s) 219, 283, 613, 757
AluI AGCT 4 cut(s) 219, 283, 613, 757
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 4 cut(s) 160, 608, 667, 709
AlwI GGATC 2 cut(s) 1136, 1177
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 2 cut(s) 216, 757
ApoI RAATTY 1 cut(s) 734
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AspLEI GCGC 2 cut(s) 1177, 1218
AspS9I GGNCC 3 cut(s) 263, 502, 563
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 4 cut(s) 832, 918, 971, 1208
AsuII TTCGAA 3 cut(s) 29, 480, 514
AvaII GGWCC 2 cut(s) 263, 563
BbsI GAAGAC 3 cut(s) 16, 59, 1089
Bbv12I GWGCWC 1 cut(s) 936
BbvI GCAGC 2 cut(s) 228, 744
BccI CCATC 3 cut(s) 206, 478, 707
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 4 cut(s) 160, 608, 667, 709
BfaI CTAG 3 cut(s) 302, 309, 1276
BfmI CTRYAG 1 cut(s) 267
BfrI CTTAAG 1 cut(s) 677
BglII AGATCT 1 cut(s) 908
BisI GCNGC 4 cut(s) 217, 758, 997, 1000
BlsI GCNGC 4 cut(s) 218, 759, 998, 1001
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 2 cut(s) 263, 563
BmgT120I GGNCC 3 cut(s) 263, 502, 563
BmrFI CCNGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 526
BpiI GAAGAC 3 cut(s) 16, 59, 1089
BplI GAGNNNNNCTC 6 cut(s) 550, 582, 568, 600, 931, 963
BpmI CTGGAG 1 cut(s) 712
Bpu14I TTCGAA 3 cut(s) 29, 480, 514
BpuEI CTTGAG 3 cut(s) 516, 593, 920
BpuMI CCSGG 1 cut(s) 491
BsaBI GATNNNNATC 2 cut(s) 19, 318
BsaHI GRCGYC 1 cut(s) 976
BsaWI WCCGGW 1 cut(s) 1125
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 988
Bse1I ACTGG 4 cut(s) 265, 729, 830, 871
Bse3DI GCAATG 1 cut(s) 1012
Bse8I GATNNNNATC 2 cut(s) 19, 318
BseGI GGATG 2 cut(s) 470, 810
BseJI GATNNNNATC 2 cut(s) 19, 318
BseLI CCNNNNNNNGG 1 cut(s) 988
BseMI GCAATG 1 cut(s) 1012
BseMII CTCAG 2 cut(s) 623, 954
BseNI ACTGG 4 cut(s) 265, 729, 830, 871
BseXI GCAGC 2 cut(s) 228, 744
Bsh1236I CGCG 3 cut(s) 557, 601, 1218
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 504
BsiEI CGRYCG 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 936
BsiSI CCGG 2 cut(s) 490, 1126
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 1 cut(s) 988
BsmAI GTCTC 4 cut(s) 160, 608, 667, 709
BsmBI CGTCTC 1 cut(s) 608
BsmFI GGGAC 1 cut(s) 737
BsnI GGCC 1 cut(s) 504
Bsp119I TTCGAA 3 cut(s) 29, 480, 514
Bsp1286I GDGCHC 1 cut(s) 936
Bsp143I GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
Bsp68I TCGCGA 1 cut(s) 557
BspACI CCGC 5 cut(s) 996, 999, 1218, 1248, 1266
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 2 cut(s) 622, 955
BspFNI CGCG 3 cut(s) 557, 601, 1218
BspPI GGATC 2 cut(s) 1136, 1177
BspQI GCTCTTC 1 cut(s) 954
BspT104I TTCGAA 3 cut(s) 29, 480, 514
BspTI CTTAAG 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 1266
BsrDI GCAATG 1 cut(s) 1012
BsrI ACTGG 4 cut(s) 265, 729, 830, 871
BssMI GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
BssNI GRCGYC 1 cut(s) 976
Bst4CI ACNGT 1 cut(s) 526
Bst6I CTCTTC 5 cut(s) 31, 511, 573, 954, 1075
BstACI GRCGYC 1 cut(s) 976
BstAFI CTTAAG 1 cut(s) 677
BstBI TTCGAA 3 cut(s) 29, 480, 514
BstC8I GCNNGC 1 cut(s) 1019
BstDEI CTNAG 2 cut(s) 609, 963
BstF5I GGATG 2 cut(s) 470, 810
BstFNI CGCG 3 cut(s) 557, 601, 1218
BstHHI GCGC 2 cut(s) 1177, 1218
BstKTI GATC 8 cut(s) 17, 322, 555, 817, 911, 1111, 1131, 1172
BstMAI GTCTC 4 cut(s) 160, 608, 667, 709
BstMBI GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 2 cut(s) 536, 876
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 3 cut(s) 557, 601, 1218
BstV1I GCAGC 2 cut(s) 228, 744
BstV2I GAAGAC 3 cut(s) 16, 59, 1089
BstX2I RGATCY 3 cut(s) 908, 1128, 1169
BstXI CCANNNNNNTGG 1 cut(s) 1158
BstYI RGATCY 3 cut(s) 908, 1128, 1169
BsuRI GGCC 1 cut(s) 504
BtsCI GGATG 2 cut(s) 470, 810
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 4 cut(s) 141, 258, 531, 736
BtuMI TCGCGA 1 cut(s) 557
Cac8I GCNNGC 1 cut(s) 1019
CfoI GCGC 2 cut(s) 1177, 1218
Cfr13I GGNCC 3 cut(s) 263, 502, 563
CseI GACGC 3 cut(s) 281, 590, 965
Csp6I GTAC 1 cut(s) 1146
CviAII CATG 3 cut(s) 162, 242, 1212
CviQI GTAC 1 cut(s) 1146
DdeI CTNAG 2 cut(s) 609, 963
DpnI GATC 8 cut(s) 16, 321, 554, 816, 910, 1110, 1130, 1171
DpnII GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
DraI TTTAAA 1 cut(s) 790
Eam1104I CTCTTC 5 cut(s) 31, 511, 573, 954, 1075
EarI CTCTTC 5 cut(s) 31, 511, 573, 954, 1075
Eco47I GGWCC 2 cut(s) 263, 563
EcoO109I RGGNCCY 1 cut(s) 502
EcoRI GAATTC 1 cut(s) 734
Esp3I CGTCTC 1 cut(s) 608
FaeI CATG 3 cut(s) 165, 245, 1215
FaqI GGGAC 1 cut(s) 737
FatI CATG 3 cut(s) 161, 241, 1211
FauI CCCGC 1 cut(s) 1259
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 4 cut(s) 217, 758, 997, 1000
FokI GGATG 2 cut(s) 457, 817
Fsp4HI GCNGC 4 cut(s) 217, 758, 997, 1000
FspBI CTAG 3 cut(s) 302, 309, 1276
GlaI GCGC 2 cut(s) 1176, 1217
GluI GCNGC 4 cut(s) 217, 758, 997, 1000
GsuI CTGGAG 1 cut(s) 712
HaeIII GGCC 1 cut(s) 504
HapII CCGG 2 cut(s) 490, 1126
HgaI GACGC 3 cut(s) 281, 590, 965
HhaI GCGC 2 cut(s) 1177, 1218
Hin1I GRCGYC 1 cut(s) 976
Hin1II CATG 3 cut(s) 165, 245, 1215
Hin6I GCGC 2 cut(s) 1175, 1216
HinP1I GCGC 2 cut(s) 1175, 1216
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 5 cut(s) 433, 583, 631, 725, 1228
HpaII CCGG 2 cut(s) 490, 1126
HphI GGTGA 4 cut(s) 832, 918, 971, 1208
Hpy166II GTNNAC 3 cut(s) 271, 926, 1142
Hpy188I TCNGA 5 cut(s) 653, 908, 964, 1227, 1244
Hpy188III TCNNGA 9 cut(s) 302, 342, 425, 533, 556, 572, 665, 937, 1132
Hpy8I GTNNAC 3 cut(s) 271, 926, 1142
Hpy99I CGWCG 1 cut(s) 1043
HpyAV CCTTC 5 cut(s) 476, 493, 515, 992, 996
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 2 cut(s) 1086, 1144
HpyCH4V TGCA 4 cut(s) 121, 293, 626, 834
HpyF10VI GCNNNNNNNGC 2 cut(s) 536, 876
HpyF3I CTNAG 2 cut(s) 609, 963
HpySE526I ACGT 2 cut(s) 1086, 1144
Hsp92I GRCGYC 1 cut(s) 976
Hsp92II CATG 3 cut(s) 165, 245, 1215
HspAI GCGC 2 cut(s) 1175, 1216
Kzo9I GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 2 cut(s) 224, 795
Lsp1109I GCAGC 2 cut(s) 228, 744
LweI GCATC 1 cut(s) 526
MaeI CTAG 3 cut(s) 302, 309, 1276
MaeII ACGT 2 cut(s) 1086, 1144
MaeIII GTNAC 3 cut(s) 520, 862, 977
MalI GATC 8 cut(s) 16, 321, 554, 816, 910, 1110, 1130, 1171
MbiI CCGCTC 1 cut(s) 1266
MboI GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
MfeI CAATTG 2 cut(s) 708, 1152
MflI RGATCY 3 cut(s) 908, 1128, 1169
MhlI GDGCHC 1 cut(s) 936
MluI ACGCGT 1 cut(s) 599
MlyI GAGTC 3 cut(s) 427, 577, 719
MmeI TCCRAC 3 cut(s) 45, 522, 631
MseI TTAA 4 cut(s) 645, 678, 783, 789
MspA1I CMGCKG 1 cut(s) 757
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 2 cut(s) 490, 1126
MspR9I CCNGG 1 cut(s) 491
MunI CAATTG 2 cut(s) 708, 1152
MvnI CGCG 3 cut(s) 557, 601, 1218
MwoI GCNNNNNNNGC 2 cut(s) 536, 876
NciI CCSGG 1 cut(s) 491
NdeII GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
NlaIII CATG 3 cut(s) 165, 245, 1215
NmuCI GTSAC 1 cut(s) 977
NruI TCGCGA 1 cut(s) 557
NspV TTCGAA 3 cut(s) 29, 480, 514
PciSI GCTCTTC 1 cut(s) 954
PfeI GAWTC 2 cut(s) 631, 1228
PkrI GCNGC 4 cut(s) 218, 759, 998, 1001
Ple19I CGATCG 1 cut(s) 17
PleI GAGTC 3 cut(s) 427, 577, 719
PpsI GAGTC 3 cut(s) 427, 577, 719
PspPI GGNCC 3 cut(s) 263, 502, 563
PsuI RGATCY 3 cut(s) 908, 1128, 1169
PvuI CGATCG 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 757
RruI TCGCGA 1 cut(s) 557
RsaI GTAC 1 cut(s) 1147
RsaNI GTAC 1 cut(s) 1146
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 4 cut(s) 645, 678, 783, 789
SatI GCNGC 4 cut(s) 217, 758, 997, 1000
Sau3AI GATC 8 cut(s) 14, 319, 552, 814, 908, 1108, 1128, 1169
Sau96I GGNCC 3 cut(s) 263, 502, 563
SchI GAGTC 3 cut(s) 427, 577, 719
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 1 cut(s) 936
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 267
SfuI TTCGAA 3 cut(s) 29, 480, 514
SinI GGWCC 2 cut(s) 263, 563
SmlI CTYRAG 4 cut(s) 531, 572, 677, 935
SmoI CTYRAG 4 cut(s) 531, 572, 677, 935
SsiI CCGC 5 cut(s) 996, 999, 1218, 1248, 1266
SspMI CTAG 3 cut(s) 302, 309, 1276
StyD4I CCNGG 1 cut(s) 489
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 2 cut(s) 1089, 1147
TaqI TCGA 6 cut(s) 17, 29, 155, 341, 480, 514
TauI GCSGC 2 cut(s) 999, 1002
TfiI GAWTC 2 cut(s) 631, 1228
Tru1I TTAA 4 cut(s) 645, 678, 783, 789
Tru9I TTAA 4 cut(s) 645, 678, 783, 789
TscAI CASTG 4 cut(s) 141, 265, 531, 736
TseFI GTSAC 1 cut(s) 977
TseI GCWGC 2 cut(s) 216, 757
Tsp45I GTSAC 1 cut(s) 977
TspDTI ATGAA 3 cut(s) 497, 892, 1001
TspRI CASTG 4 cut(s) 141, 265, 531, 736
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 2 cut(s) 263, 563
XapI RAATTY 1 cut(s) 734
XbaI TCTAGA 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 3 cut(s) 302, 309, 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.