Rroxscaffold_4G00291490

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018419
Physical Location & Seq
Reverse (-)
11926077 .. 11927763
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00291490.1

Sequence Viewer

Length: 867 bp
ATGACCTTATTAAAGCTCCGTGCGCAAACCCCTAACCCCCACTTTCGCATTGCGCAATTGACGATTGACGAGTCCATAATCTCCACTTTCCATGTGAACGGATCTGACGCTTTCAAGCTTTCTACTGCAAGAGGTCTGTTAGACACTAGAGATATTGATCTATGGATTTCTCGTCTATCGAGTTACTCTATCAAGGAGTTTATACTGAAATTATGGAATAGGAGCTTCAAATACGATGTCTTTTCCCGTTTGTTTTCTTTTCAAGATTTGACTCTCTTGGAGTTATGCAATTGTTCCCTAAAACCTCCGTCCACATTCAAAGGCTTCCGGGTGTTGAAGGGCCTTCATATTCGAAAAGTTACAGTGGCTCAAGATGCGTTGGAAAAACTGATCGAGAGTGGTCCTCTTCTTGAGAGAATGACTCTATGTGACATAACGCGTCTCACTCAGCTCAACATTTATGCACAGAATCTCCAATTCCTTAAAGTCGGAGGTGTTTTTCAGGATTTCAGACTTAAGAATACCACAAATCTGGTTGATGTTTCAATTGATGGAAAATGGGACTCCAGTGAATTCTTCAACAGGATGAGGTTAGATCAGCCCAACTGGAATTGCACATTCACCCAACTTCAAGTTTTGAAAGTAACTGGCTATTTTGGCATACACACAGAGGAAGTAGAGTTCATCAGATCTGTGCTTTCAAGTTCACCTGTGCTCAAGAGGATTGCTCTTCAACCTGTTTCTGGTACCAATGTCTGTTGGGAACTGCCTATAATGTTGAACGATTTTGGGAATGTAGAGAAAAATTTCTTGGCCCCGTTAACTGATTACTATATGCAATCTAATGAGTATTATGATGAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

288

Amino Acids

33.29

Weight (kDa)

6.9

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 37 - 166 3e-11 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 53 - 180 3.6e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018419)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17800
rosa_chinensis RchiOBHm_Chr1g0365081
rosa_laevigata RLG00000027446
rosa_multiflora Rmu_sc0022857.1_g000001 Rmu_sc0034521.1_g000001
rosa_roxburghii Rroxscaffold_4G00291490 Rroxscaffold_4G00291940
rosa_rugosa Rorug01G0319400
rosa_samantha Rh1AG328400 Rh1DG321400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 24, 54
Acc65I GGTACC 1 cut(s) 746
AccB1I GGYRCC 1 cut(s) 746
AccII CGCG 1 cut(s) 439
AclWI GGATC 1 cut(s) 109
AcsI RAATTY 2 cut(s) 572, 805
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 1 cut(s) 743
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 437
AjuI GAANNNNNNNTTGG 2 cut(s) 794, 826
AloI GAACNNNNNNTCC 2 cut(s) 665, 697
AluBI AGCT 4 cut(s) 16, 118, 225, 451
AluI AGCT 4 cut(s) 16, 118, 225, 451
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 1 cut(s) 446
AlwI GGATC 1 cut(s) 109
AoxI GGCC 2 cut(s) 340, 813
ApoI RAATTY 2 cut(s) 572, 805
ArsI GACNNNNNNTTYG 2 cut(s) 222, 254
Asp700I GAANNNNTTC 1 cut(s) 806
Asp718I GGTACC 1 cut(s) 746
AspLEI GCGC 2 cut(s) 25, 55
AspS9I GGNCC 3 cut(s) 340, 401, 814
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 2 cut(s) 613, 699
AsuII TTCGAA 1 cut(s) 352
AvaII GGWCC 1 cut(s) 401
BaeI ACNNNNGTAYC 2 cut(s) 738, 771
BanI GGYRCC 1 cut(s) 746
Bbv12I GWGCWC 1 cut(s) 717
BccI CCATC 1 cut(s) 545
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 1 cut(s) 446
BfaI CTAG 1 cut(s) 147
BfrI CTTAAG 1 cut(s) 515
BglII AGATCT 1 cut(s) 689
Bme1390I CCNGG 1 cut(s) 329
Bme18I GGWCC 1 cut(s) 401
BmgT120I GGNCC 3 cut(s) 340, 401, 814
BmiI GGNNCC 2 cut(s) 748, 816
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 1 cut(s) 364
BplI GAGNNNNNCTC 6 cut(s) 388, 420, 406, 438, 712, 744
BpmI CTGGAG 1 cut(s) 550
Bpu14I TTCGAA 1 cut(s) 352
BpuEI CTTGAG 3 cut(s) 354, 431, 701
BpuMI CCSGG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 743
Bse1I ACTGG 3 cut(s) 567, 611, 652
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 156
BseGI GGATG 1 cut(s) 591
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 743
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 3 cut(s) 567, 611, 652
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 2 cut(s) 342, 815
BshNI GGYRCC 1 cut(s) 746
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 1 cut(s) 328
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 1 cut(s) 743
BsmAI GTCTC 1 cut(s) 446
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 2 cut(s) 342, 815
Bsp119I TTCGAA 1 cut(s) 352
Bsp1286I GDGCHC 1 cut(s) 717
Bsp143I GATC 5 cut(s) 101, 157, 390, 595, 689
BspANI GGCC 2 cut(s) 342, 815
BspCNI CTCAG 1 cut(s) 460
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 748, 816
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 1 cut(s) 735
BspT104I TTCGAA 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 746
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 48
BsrI ACTGG 3 cut(s) 567, 611, 652
BssMI GATC 5 cut(s) 101, 157, 390, 595, 689
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 2 cut(s) 411, 735
BstAFI CTTAAG 1 cut(s) 515
BstBI TTCGAA 1 cut(s) 352
BstDEI CTNAG 1 cut(s) 447
BstF5I GGATG 1 cut(s) 591
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 2 cut(s) 25, 55
BstKTI GATC 5 cut(s) 104, 160, 393, 598, 692
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 5 cut(s) 101, 157, 390, 595, 689
BstMWI GCNNNNNNNGC 3 cut(s) 22, 374, 657
BstSCI CCNGG 1 cut(s) 327
BstUI CGCG 1 cut(s) 439
BstX2I RGATCY 2 cut(s) 101, 689
BstXI CCANNNNNNTGG 1 cut(s) 532
BstYI RGATCY 2 cut(s) 101, 689
BsuRI GGCC 2 cut(s) 342, 815
BtsCI GGATG 1 cut(s) 591
BtsIMutI CAGTG 2 cut(s) 369, 574
CfoI GCGC 2 cut(s) 25, 55
Cfr13I GGNCC 3 cut(s) 340, 401, 814
CseI GACGC 2 cut(s) 116, 428
Csp6I GTAC 1 cut(s) 747
CviAII CATG 1 cut(s) 92
CviQI GTAC 1 cut(s) 747
DdeI CTNAG 1 cut(s) 447
DpnI GATC 5 cut(s) 103, 159, 392, 597, 691
DpnII GATC 5 cut(s) 101, 157, 390, 595, 689
Eam1104I CTCTTC 2 cut(s) 411, 735
EarI CTCTTC 2 cut(s) 411, 735
Eco47I GGWCC 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 340
EcoRI GAATTC 1 cut(s) 572
Esp3I CGTCTC 1 cut(s) 446
FaeI CATG 1 cut(s) 95
FaqI GGGAC 1 cut(s) 575
FatI CATG 1 cut(s) 91
FokI GGATG 1 cut(s) 598
FspBI CTAG 1 cut(s) 147
FspI TGCGCA 2 cut(s) 24, 54
GlaI GCGC 2 cut(s) 24, 54
GsuI CTGGAG 1 cut(s) 550
HaeIII GGCC 2 cut(s) 342, 815
HapII CCGG 1 cut(s) 328
HgaI GACGC 2 cut(s) 116, 428
HhaI GCGC 2 cut(s) 25, 55
Hin1II CATG 1 cut(s) 95
Hin6I GCGC 2 cut(s) 23, 53
HinP1I GCGC 2 cut(s) 23, 53
HincII GTYRAC 1 cut(s) 822
HindII GTYRAC 1 cut(s) 822
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 5 cut(s) 71, 271, 421, 469, 563
HpaI GTTAAC 1 cut(s) 822
HpaII CCGG 1 cut(s) 328
HphI GGTGA 2 cut(s) 613, 699
Hpy166II GTNNAC 4 cut(s) 97, 312, 707, 822
Hpy188I TCNGA 4 cut(s) 106, 491, 512, 689
Hpy188III TCNNGA 6 cut(s) 263, 371, 394, 410, 503, 718
Hpy8I GTNNAC 4 cut(s) 97, 312, 707, 822
HpyAV CCTTC 2 cut(s) 331, 353
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4V TGCA 5 cut(s) 128, 288, 464, 615, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 374, 657
HpyF3I CTNAG 1 cut(s) 447
Hsp92II CATG 1 cut(s) 95
HspAI GCGC 2 cut(s) 23, 53
KpnI GGTACC 1 cut(s) 750
KspAI GTTAAC 1 cut(s) 822
Kzo9I GATC 5 cut(s) 101, 157, 390, 595, 689
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 2 cut(s) 21, 222
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 147
MaeIII GTNAC 4 cut(s) 182, 358, 428, 643
MalI GATC 5 cut(s) 103, 159, 392, 597, 691
MboI GATC 5 cut(s) 101, 157, 390, 595, 689
MboII GAAGA 3 cut(s) 398, 568, 722
MfeI CAATTG 3 cut(s) 56, 289, 546
MflI RGATCY 2 cut(s) 101, 689
MhlI GDGCHC 1 cut(s) 717
MluCI AATT 8 cut(s) 56, 209, 289, 476, 546, 572, 610, 805
MluI ACGCGT 1 cut(s) 437
MlyI GAGTC 4 cut(s) 80, 265, 415, 557
MmeI TCCRAC 2 cut(s) 360, 469
MnlI CCTC 8 cut(s) 125, 315, 414, 485, 582, 664, 714, 853
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 4 cut(s) 11, 483, 516, 821
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 1 cut(s) 329
MunI CAATTG 3 cut(s) 56, 289, 546
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 3 cut(s) 22, 374, 657
NciI CCSGG 1 cut(s) 329
NdeII GATC 5 cut(s) 101, 157, 390, 595, 689
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 2 cut(s) 748, 816
NmuCI GTSAC 1 cut(s) 428
NsbI TGCGCA 2 cut(s) 24, 54
NspV TTCGAA 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 1 cut(s) 469
PleI GAGTC 4 cut(s) 79, 265, 415, 557
PpsI GAGTC 4 cut(s) 79, 265, 415, 557
PspN4I GGNNCC 2 cut(s) 748, 816
PspPI GGNCC 3 cut(s) 340, 401, 814
PsuI RGATCY 2 cut(s) 101, 689
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 4 cut(s) 11, 483, 516, 821
Sau3AI GATC 5 cut(s) 101, 157, 390, 595, 689
Sau96I GGNCC 3 cut(s) 340, 401, 814
SchI GAGTC 4 cut(s) 80, 265, 415, 557
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 1 cut(s) 717
SfaNI GCATC 1 cut(s) 364
SfuI TTCGAA 1 cut(s) 352
SinI GGWCC 1 cut(s) 401
SmlI CTYRAG 4 cut(s) 369, 410, 515, 716
SmoI CTYRAG 4 cut(s) 369, 410, 515, 716
Sse9I AATT 8 cut(s) 56, 209, 289, 476, 546, 572, 610, 805
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 364
TaqI TCGA 3 cut(s) 179, 352, 393
TasI AATT 8 cut(s) 56, 209, 289, 476, 546, 572, 610, 805
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 4 cut(s) 11, 483, 516, 821
Tru9I TTAA 4 cut(s) 11, 483, 516, 821
TscAI CASTG 2 cut(s) 369, 574
TseFI GTSAC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 428
TspDTI ATGAA 2 cut(s) 335, 673
TspGWI ACGGA 3 cut(s) 8, 114, 297
TspRI CASTG 2 cut(s) 369, 574
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 2 cut(s) 572, 805
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.