RLG00000028332

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017768
Physical Location & Seq
Reverse (-)
21487477 .. 21489768
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028332

Sequence Viewer

Length: 1356 bp
ATGGTGAACATTGAGGTCACTGTTATTTCCAGAGACACCATTAAACCATCAACTGCTTCCTCTTCGCCTCACCTTCTACAACCTTACAAGCTTTCCATTTTCGATCAGCTCATTCCCAACACTTACGTCCCGATGATTTTCTTCTACCCCATTACTGTCCCCAGCTTCAATCTCCCTCAAACCCTAATCCACTTGAGAAACTCCCTCTCCGAGACCCTCACTCTGTACTACCCATTGTCCGGAAGGATAAAAAACAACCTCTACGTACATGACTTCGACGCGGGTGTCCCCTACCTTGAAGCTCAAGTCAACTGTCCCATGCTTGAGTTTCTTAAGCTTCGAGAAACCGAATGGCTAAACCACTTCGTACCATTCCATCCATTTCTCAAGGAAATGGATTCTGAGCTACTGCCCCTCATCGCATGTCAAGCCAACATCTTCGATGGTGGAATAGCAATGGGCATTTCCCTTTCTCACAAGCTCTCTGATGGGGTAACAGCCAATGCTTTTCTCAAGACTTGGGCTGCTATTTTTCGTGGCTGTCGCCAAGAAATCATATACCCTAATCTCTTCCAAGCCTCATTGGTTTTTCCACCACGAGATAACTTGCCCAAAAGGTATGTAGCTCTCATGGAGAGGTTGTGGTTCGAGCAGAAAAAGGATTACGTTACAAGGAGATTTCTGTTTGATGCTAAAGCCACATCCACACTTCAAGTCAAAGCAAAGAGCGAGCTCGTGCCAAAACCATCACGTATTGAGGCACTTACATGTTTTCTCTGGAAACATCAAATGGCTGCCTCTCGGGCATCGTCATCAGGTACTTCTCCAAAACTCTCCATAGTAGCGCATGCAGTAAACTTGAGGCCACGAATGAACCCACAAATGTTGTTGGATAATACCATTGGGAATATCTTTTTGTGGGCTCCGGTGTTATTAGATATCTTGGAAGAGACAACAACAAAGAGTAGTGATCATGAACTGTGTGACTTGGTGAACATGCTTCATGAATCCCTCAACGAATTTAACAATGAGTACTTGGAGACTCTGAAGGGTAAAGAAGGATTTGGAGCCATTTGTGACTTACTAGATTTGATGGCGGAAGGGACTTCTATAAAACCACCACCAGAGATTTATGCATTCACGAGCTGGACTAGAATTCTTAACAAACTAGATTTTGGATGGGGAAAGCCATTTCAGATTGGAGTCATTGGAAAAGTTGGACCTGTATTTAGAAATCTAACAATATTCGTACAATGCGATCAAGAAATTGAAGCATGGGTAACTCTGGAGGAAAAACAAATGGCTATGCTAGAAAAAGATTCTCAATTTCTAGCCTTCGCATCTCCAAATCCCTAA

Protein Analysis

452

Amino Acids

51.26

Weight (kDa)

6.1

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 443 8.6e-66 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccII CGCG 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 2 cut(s) 281, 1097
AcsI RAATTY 2 cut(s) 1019, 1155
AcuI CTGAAG 1 cut(s) 1067
AfaI GTAC 6 cut(s) 227, 267, 369, 820, 1034, 1251
AfiI CCNNNNNNNGG 1 cut(s) 239
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 4 cut(s) 169, 299, 713, 1271
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 4 cut(s) 27, 206, 944, 1034
Ama87I CYCGRG 1 cut(s) 801
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 1 cut(s) 863
ApeKI GCWGC 2 cut(s) 524, 794
ApoI RAATTY 2 cut(s) 1019, 1155
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 1 cut(s) 1220
AsuHPI GGTGA 3 cut(s) 16, 62, 1003
AvaI CYCGRG 1 cut(s) 801
AvaII GGWCC 1 cut(s) 1220
BanII GRGCYC 2 cut(s) 735, 925
BauI CACGAG 3 cut(s) 597, 734, 1141
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 2 cut(s) 511, 781
BccI CCATC 7 cut(s) 55, 384, 437, 482, 754, 1087, 1173
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 4 cut(s) 27, 206, 944, 1034
BfaI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
BfrI CTTAAG 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 803
BisI GCNGC 2 cut(s) 525, 795
BlsI GCNGC 2 cut(s) 526, 796
BmcAI AGTACT 1 cut(s) 1034
Bme18I GGWCC 1 cut(s) 1220
BmeT110I CYCGRG 1 cut(s) 801
BmgT120I GGNCC 1 cut(s) 1220
BmiI GGNNCC 2 cut(s) 924, 1069
BmsI GCATC 3 cut(s) 679, 815, 1349
BpmI CTGGAG 1 cut(s) 1307
BpuEI CTTGAG 6 cut(s) 214, 288, 344, 371, 497, 880
BsaAI YACGTR 2 cut(s) 265, 752
BsaI GGTCTC 1 cut(s) 206
BsaWI WCCGGW 2 cut(s) 239, 925
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 239
BseGI GGATG 3 cut(s) 376, 701, 1184
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 1 cut(s) 393
BseXI GCAGC 2 cut(s) 511, 781
BseYI CCCAGC 1 cut(s) 161
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 865
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 801
BsiSI CCGG 2 cut(s) 240, 926
BslFI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 4 cut(s) 27, 206, 944, 1034
BsmFI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
BsmI GAATGC 1 cut(s) 1136
BsnI GGCC 1 cut(s) 865
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 801
Bsp1286I GDGCHC 2 cut(s) 735, 925
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 3 cut(s) 103, 970, 1258
BspACI CCGC 2 cut(s) 281, 1097
BspANI GGCC 1 cut(s) 865
BspCNI CTCAG 1 cut(s) 394
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 2 cut(s) 973, 1003
BspLI GGNNCC 2 cut(s) 924, 1069
BspTI CTTAAG 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 462
BssMI GATC 3 cut(s) 103, 970, 1258
BssSI CACGAG 3 cut(s) 597, 734, 1141
Bst2BI CACGAG 3 cut(s) 597, 734, 1141
Bst4CI ACNGT 4 cut(s) 22, 157, 314, 981
Bst6I CTCTTC 3 cut(s) 67, 575, 942
BstAFI CTTAAG 1 cut(s) 332
BstBAI YACGTR 2 cut(s) 265, 752
BstC8I GCNNGC 2 cut(s) 731, 849
BstDEI CTNAG 1 cut(s) 402
BstF5I GGATG 3 cut(s) 376, 701, 1184
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 847
BstKTI GATC 3 cut(s) 106, 973, 1261
BstMAI GTCTC 4 cut(s) 27, 206, 944, 1034
BstMBI GATC 3 cut(s) 103, 970, 1258
BstMWI GCNNNNNNNGC 2 cut(s) 428, 803
BstNSI RCATGY 4 cut(s) 426, 771, 851, 1000
BstSNI TACGTA 1 cut(s) 265
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 511, 781
BsuRI GGCC 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 403
BtsCI GGATG 3 cut(s) 376, 701, 1184
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 731, 849
CciI TCATGA 2 cut(s) 973, 1003
CfoI GCGC 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 1220
CseI GACGC 1 cut(s) 287
Csp6I GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
CspCI CAANNNNNGTGG 2 cut(s) 867, 902
CviQI GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
DdeI CTNAG 1 cut(s) 402
DpnI GATC 3 cut(s) 105, 972, 1260
DpnII GATC 3 cut(s) 103, 970, 1258
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 67, 575, 942
EarI CTCTTC 3 cut(s) 67, 575, 942
EciI GGCGGA 1 cut(s) 1112
Ecl136II GAGCTC 1 cut(s) 733
Eco105I TACGTA 1 cut(s) 265
Eco24I GRGCYC 2 cut(s) 735, 925
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 1 cut(s) 1220
Eco53kI GAGCTC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 1067
Eco88I CYCGRG 1 cut(s) 801
EcoICRI GAGCTC 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 1155
EcoRV GATATC 1 cut(s) 940
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 2 cut(s) 735, 925
FaqI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
FauI CCCGC 1 cut(s) 274
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 2 cut(s) 525, 795
FokI GGATG 3 cut(s) 363, 688, 1191
FriOI GRGCYC 2 cut(s) 735, 925
Fsp4HI GCNGC 2 cut(s) 525, 795
FspBI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
GlaI GCGC 1 cut(s) 846
GluI GCNGC 2 cut(s) 525, 795
GsaI CCCAGC 1 cut(s) 165
GsuI CTGGAG 1 cut(s) 1307
HaeIII GGCC 1 cut(s) 865
HapII CCGG 2 cut(s) 240, 926
HgaI GACGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 847
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 2 cut(s) 89, 335
HinfI GANTC 5 cut(s) 398, 1007, 1042, 1203, 1319
HpaII CCGG 2 cut(s) 240, 926
HphI GGTGA 3 cut(s) 16, 62, 1003
Hpy166II GTNNAC 4 cut(s) 7, 310, 856, 994
Hpy188I TCNGA 5 cut(s) 211, 403, 487, 1047, 1197
Hpy8I GTNNAC 4 cut(s) 7, 310, 856, 994
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 6 cut(s) 83, 237, 1042, 1052, 1094, 1345
HpyCH4III ACNGT 4 cut(s) 22, 157, 314, 981
HpyCH4IV ACGT 4 cut(s) 126, 264, 666, 751
HpyCH4V TGCA 2 cut(s) 851, 1136
HpyF10VI GCNNNNNNNGC 2 cut(s) 428, 803
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 4 cut(s) 126, 264, 666, 751
HspAI GCGC 1 cut(s) 845
Kpn2I TCCGGA 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 3 cut(s) 103, 970, 1258
LmnI GCTCC 2 cut(s) 928, 1067
Lsp1109I GCAGC 2 cut(s) 511, 781
LweI GCATC 3 cut(s) 679, 815, 1349
MaeI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
MaeII ACGT 4 cut(s) 126, 264, 666, 751
MaeIII GTNAC 6 cut(s) 16, 493, 667, 983, 1076, 1279
MalI GATC 3 cut(s) 105, 972, 1260
MboI GATC 3 cut(s) 103, 970, 1258
MboII GAAGA 5 cut(s) 54, 133, 430, 562, 959
MhlI GDGCHC 2 cut(s) 735, 925
MluCI AATT 4 cut(s) 1019, 1155, 1266, 1325
MlyI GAGTC 2 cut(s) 1036, 1212
MmeI TCCRAC 2 cut(s) 870, 1198
Mph1103I ATGCAT 1 cut(s) 1138
MroI TCCGGA 1 cut(s) 239
MseI TTAA 4 cut(s) 42, 333, 1023, 1161
MslI CAYNNNNRTG 1 cut(s) 766
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 2 cut(s) 240, 926
Mva1269I GAATGC 1 cut(s) 1136
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 428, 803
NdeII GATC 3 cut(s) 103, 970, 1258
NlaIV GGNNCC 2 cut(s) 924, 1069
NmuCI GTSAC 3 cut(s) 16, 983, 1076
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 4 cut(s) 426, 771, 851, 1000
PaeI GCATGC 1 cut(s) 851
PagI TCATGA 2 cut(s) 973, 1003
PciI ACATGT 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 1254
PctI GAATGC 1 cut(s) 1136
PfeI GAWTC 3 cut(s) 398, 1007, 1319
PkrI GCNGC 2 cut(s) 526, 796
PleI GAGTC 2 cut(s) 1036, 1211
PpsI GAGTC 2 cut(s) 1036, 1211
Ppu21I YACGTR 2 cut(s) 265, 752
PscI ACATGT 1 cut(s) 767
Psp124BI GAGCTC 1 cut(s) 735
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 924, 1069
PspPI GGNCC 1 cut(s) 1220
RsaI GTAC 6 cut(s) 227, 267, 369, 820, 1034, 1251
RsaNI GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
RseI CAYNNNNRTG 1 cut(s) 766
SacI GAGCTC 1 cut(s) 735
SaqAI TTAA 4 cut(s) 42, 333, 1023, 1161
SatI GCNGC 2 cut(s) 525, 795
Sau3AI GATC 3 cut(s) 103, 970, 1258
Sau96I GGNCC 1 cut(s) 1220
ScaI AGTACT 1 cut(s) 1034
SchI GAGTC 2 cut(s) 1036, 1212
SduI GDGCHC 2 cut(s) 735, 925
SfaNI GCATC 3 cut(s) 679, 815, 1349
SinI GGWCC 1 cut(s) 1220
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 7 cut(s) 193, 303, 323, 332, 386, 512, 859
SmoI CTYRAG 7 cut(s) 193, 303, 323, 332, 386, 512, 859
SnaBI TACGTA 1 cut(s) 265
SphI GCATGC 1 cut(s) 851
Sse9I AATT 4 cut(s) 1019, 1155, 1266, 1325
SsiI CCGC 2 cut(s) 281, 1097
SspI AATATT 1 cut(s) 1245
SspMI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
SstI GAGCTC 1 cut(s) 735
TaaI ACNGT 4 cut(s) 22, 157, 314, 981
TaiI ACGT 4 cut(s) 129, 267, 669, 754
TaqI TCGA 5 cut(s) 102, 276, 340, 441, 648
TasI AATT 4 cut(s) 1019, 1155, 1266, 1325
TatI WGTACW 2 cut(s) 225, 1032
TfiI GAWTC 3 cut(s) 398, 1007, 1319
Tru1I TTAA 4 cut(s) 42, 333, 1023, 1161
Tru9I TTAA 4 cut(s) 42, 333, 1023, 1161
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 3 cut(s) 16, 983, 1076
TseI GCWGC 2 cut(s) 524, 794
Tsp45I GTSAC 3 cut(s) 16, 983, 1076
TspDTI ATGAA 4 cut(s) 887, 990, 992, 1020
TspRI CASTG 1 cut(s) 25
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 1220
XapI RAATTY 2 cut(s) 1019, 1155
XceI RCATGY 4 cut(s) 426, 771, 851, 1000
XspI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
ZrmI AGTACT 1 cut(s) 1034
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.