Rh1DG239900

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017768
Physical Location & Seq
Forward (+)
45533429 .. 45537928
4500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG239900.1

Sequence Viewer

Length: 1356 bp
ATGGTGAACATTAAGGTAACTGTTATTTCCAGAGACACCATTAAACCATCAACTGCTTCCTCTTCGCCTCACCTTCTACAACCTTACAAGCTTTCCATTTTCGATCAGCTCATTCCCAACACTTACGTCCCAATGATTTTCTTCTACCCCATTACTGTCCCCAGCTTCAATCTCCCTCAAACCCTAATCCACTTGAGAAGCTCCCTCTCCGAGACCCTCACTCTGTACTACCCATTGTCCGGAAGGATAAAAAACAACCTCTACGTACATGACTTCGACGCGGGTGTCCCCTACCTTGAAGCTCAAGTCAACTGTCCCATGCTCGAGTTTCCTAAGCTTCGAGAAACCGAATGGCTAAACCACTTCGTACCATTCCATCCATTTCTCAAGGAAATGGATTCTGAGCTACTGCCCCTCATCGCATGTCAAGCCAACATCTTCGATGGTGGAATAGCAATGGGCATTTCCCTTTCTCACAAGCTCTCTGATGGGGTAACAGCCAATGCTTTTCTCAAGACTTGGGCTGCTATTTTTCGTGGCTGTCGCCAAGAAATCATATACCCTAATCTCTTCCAAGCCTCATTGGTTTTTCCACCACGAGATAACTTGCCTAAAAGGTATGTAGCTCTCATGGAGAGGTTGTGGTTCGAGCAGAAAAAGGATTACGTTACAAGGAGATTTCTGTTTGATGCTAAAGCCATATCCGCACTTCAAGTCAAAGCAAATAGCGAACTCGTGCCCAAACCATCACGTATTGAGGCACTTACATGTTTTCTCTGGAAACATCAAATGGCTGCCTCTCGGGCATCGTCATCAGGTACTTCTTCAAAACTCTCCATAGTAGCGCATGCGGTAAACCTGAGGCCACGAATGAACCCGCAAATGTTGTTGGATAATACCATTGGGAATATCTTTTTGTGGGCTCCGGTGTTATTAGATATCTTGGAAGAGACAGCAACAAAGAGTAGTGATCATGAACTGTGTGACTTGGTGAACATGCTTCATGAATCCCTCAATGAATTTAACAATGAGTACTTGGAGACTCTGAAGGGTAAAGAAGGATTTGGAGCCATTTGTGACTTACTAGATTTGATGGAGGAAGGGACTTCTATAAAACCACCGCCAGAGATTTATGCATTCACGAGCTGGACTAGAATTCTTAACAAACTAGATTTTGGATGGGGAAAGCCATTTCAGATTGGAGTCATCGGAAAAGTTGGACCTATATTTAGAAATCTAACGATATTCGTACAATGCGATCAAGAAATTGAAGCATGGGTGACTTTGGAGGAAAAACAAATGGCTATGCTAGAAAAAGATTCTCAATTTCTAGCGTTTGCATCTCCAAATCCCTAA

Protein Analysis

451

Amino Acids

51.21

Weight (kDa)

6.1

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 443 1.2e-66 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccII CGCG 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 5 cut(s) 281, 705, 851, 878, 1121
AcsI RAATTY 2 cut(s) 1019, 1155
AcuI CTGAAG 1 cut(s) 1067
AfaI GTAC 6 cut(s) 227, 267, 369, 820, 1034, 1251
AfiI CCNNNNNNNGG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 5 cut(s) 169, 299, 713, 828, 1271
Alw26I GTCTC 4 cut(s) 27, 206, 944, 1034
Ama87I CYCGRG 2 cut(s) 323, 801
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 1 cut(s) 863
ApeKI GCWGC 2 cut(s) 524, 794
ApoI RAATTY 2 cut(s) 1019, 1155
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 1 cut(s) 1220
AsuHPI GGTGA 4 cut(s) 16, 62, 1003, 1291
AvaI CYCGRG 2 cut(s) 323, 801
AvaII GGWCC 1 cut(s) 1220
AxyI CCTNAGG 1 cut(s) 860
BaeGI GKGCMC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 925
BauI CACGAG 3 cut(s) 597, 734, 1141
BbvI GCAGC 2 cut(s) 511, 781
BccI CCATC 7 cut(s) 55, 384, 437, 482, 754, 1087, 1173
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 4 cut(s) 27, 206, 944, 1034
BfaI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
BglI GCCNNNNNGGC 1 cut(s) 803
BisI GCNGC 2 cut(s) 525, 795
BlsI GCNGC 2 cut(s) 526, 796
BmcAI AGTACT 1 cut(s) 1034
Bme18I GGWCC 1 cut(s) 1220
BmeT110I CYCGRG 2 cut(s) 323, 801
BmgT120I GGNCC 1 cut(s) 1220
BmiI GGNNCC 2 cut(s) 924, 1069
BmsI GCATC 3 cut(s) 679, 815, 1349
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 4 cut(s) 214, 288, 371, 497
BsaAI YACGTR 2 cut(s) 265, 752
BsaI GGTCTC 1 cut(s) 206
BsaWI WCCGGW 2 cut(s) 239, 925
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse21I CCTNAGG 1 cut(s) 860
Bse3DI GCAATG 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 239
BseGI GGATG 2 cut(s) 376, 1184
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 2 cut(s) 393, 851
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 2 cut(s) 511, 781
BseYI CCCAGC 1 cut(s) 161
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 865
BsiHKCI CYCGRG 2 cut(s) 323, 801
BsiSI CCGG 2 cut(s) 240, 926
BslFI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 4 cut(s) 27, 206, 944, 1034
BsmFI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
BsmI GAATGC 1 cut(s) 1136
BsnI GGCC 1 cut(s) 865
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 2 cut(s) 323, 801
Bsp1286I GDGCHC 2 cut(s) 741, 925
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 3 cut(s) 103, 970, 1258
BspACI CCGC 5 cut(s) 281, 705, 851, 878, 1121
BspANI GGCC 1 cut(s) 865
BspCNI CTCAG 2 cut(s) 394, 852
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 2 cut(s) 973, 1003
BspLI GGNNCC 2 cut(s) 924, 1069
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 462
BssMI GATC 3 cut(s) 103, 970, 1258
BssSI CACGAG 3 cut(s) 597, 734, 1141
Bst2BI CACGAG 3 cut(s) 597, 734, 1141
Bst4CI ACNGT 4 cut(s) 22, 157, 314, 981
Bst6I CTCTTC 3 cut(s) 67, 575, 942
BstBAI YACGTR 2 cut(s) 265, 752
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 3 cut(s) 333, 402, 860
BstF5I GGATG 2 cut(s) 376, 1184
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 847
BstKTI GATC 3 cut(s) 106, 973, 1261
BstMAI GTCTC 4 cut(s) 27, 206, 944, 1034
BstMBI GATC 3 cut(s) 103, 970, 1258
BstMWI GCNNNNNNNGC 3 cut(s) 428, 704, 803
BstNSI RCATGY 4 cut(s) 426, 771, 851, 1000
BstSLI GKGCMC 1 cut(s) 741
BstSNI TACGTA 1 cut(s) 265
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 511, 781
Bsu36I CCTNAGG 1 cut(s) 860
BsuRI GGCC 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 403
BtsCI GGATG 2 cut(s) 376, 1184
Cac8I GCNNGC 1 cut(s) 849
CciI TCATGA 2 cut(s) 973, 1003
CfoI GCGC 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 1220
CseI GACGC 1 cut(s) 287
Csp6I GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
CviQI GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
DdeI CTNAG 3 cut(s) 333, 402, 860
DpnI GATC 3 cut(s) 105, 972, 1260
DpnII GATC 3 cut(s) 103, 970, 1258
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 67, 575, 942
EarI CTCTTC 3 cut(s) 67, 575, 942
Eco105I TACGTA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 925
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 1 cut(s) 1220
Eco57I CTGAAG 1 cut(s) 1067
Eco81I CCTNAGG 1 cut(s) 860
Eco88I CYCGRG 2 cut(s) 323, 801
EcoRI GAATTC 1 cut(s) 1155
EcoRV GATATC 1 cut(s) 940
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 925
FaqI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
FauI CCCGC 2 cut(s) 274, 885
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 2 cut(s) 525, 795
FokI GGATG 2 cut(s) 363, 1191
FriOI GRGCYC 1 cut(s) 925
Fsp4HI GCNGC 2 cut(s) 525, 795
FspBI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
GlaI GCGC 1 cut(s) 846
GluI GCNGC 2 cut(s) 525, 795
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 865
HapII CCGG 2 cut(s) 240, 926
HgaI GACGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 847
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 2 cut(s) 89, 335
HinfI GANTC 5 cut(s) 398, 1007, 1042, 1203, 1319
HpaII CCGG 2 cut(s) 240, 926
HphI GGTGA 4 cut(s) 16, 62, 1003, 1291
Hpy166II GTNNAC 4 cut(s) 7, 310, 856, 994
Hpy188I TCNGA 6 cut(s) 211, 403, 487, 1047, 1197, 1211
Hpy188III TCNNGA 9 cut(s) 30, 240, 341, 514, 778, 974, 1004, 1141, 1262
Hpy8I GTNNAC 4 cut(s) 7, 310, 856, 994
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 5 cut(s) 83, 237, 1042, 1052, 1094
HpyCH4III ACNGT 4 cut(s) 22, 157, 314, 981
HpyCH4IV ACGT 4 cut(s) 126, 264, 666, 751
HpyCH4V TGCA 2 cut(s) 1136, 1340
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 704, 803
HpyF3I CTNAG 3 cut(s) 333, 402, 860
HpySE526I ACGT 4 cut(s) 126, 264, 666, 751
HspAI GCGC 1 cut(s) 845
Kpn2I TCCGGA 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 3 cut(s) 103, 970, 1258
LmnI GCTCC 3 cut(s) 206, 928, 1067
LpnPI CCDG 9 cut(s) 43, 175, 253, 763, 801, 872, 939, 1132, 1137
Lsp1109I GCAGC 2 cut(s) 511, 781
LweI GCATC 3 cut(s) 679, 815, 1349
MaeI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
MaeII ACGT 4 cut(s) 126, 264, 666, 751
MaeIII GTNAC 6 cut(s) 16, 493, 667, 983, 1076, 1279
MalI GATC 3 cut(s) 105, 972, 1260
MboI GATC 3 cut(s) 103, 970, 1258
MboII GAAGA 6 cut(s) 54, 133, 430, 562, 816, 959
MhlI GDGCHC 2 cut(s) 741, 925
MluCI AATT 4 cut(s) 1019, 1155, 1266, 1325
MlyI GAGTC 2 cut(s) 1036, 1212
MmeI TCCRAC 2 cut(s) 870, 1198
Mph1103I ATGCAT 1 cut(s) 1138
MroI TCCGGA 1 cut(s) 239
MseI TTAA 4 cut(s) 12, 42, 1023, 1161
MslI CAYNNNNRTG 1 cut(s) 766
MspI CCGG 2 cut(s) 240, 926
Mva1269I GAATGC 1 cut(s) 1136
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 3 cut(s) 428, 704, 803
NdeII GATC 3 cut(s) 103, 970, 1258
NlaIV GGNNCC 2 cut(s) 924, 1069
NmuCI GTSAC 3 cut(s) 983, 1076, 1279
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 4 cut(s) 426, 771, 851, 1000
PaeI GCATGC 1 cut(s) 851
PaeR7I CTCGAG 1 cut(s) 323
PagI TCATGA 2 cut(s) 973, 1003
PciI ACATGT 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 1254
PctI GAATGC 1 cut(s) 1136
PfeI GAWTC 3 cut(s) 398, 1007, 1319
PkrI GCNGC 2 cut(s) 526, 796
PleI GAGTC 2 cut(s) 1036, 1211
PpsI GAGTC 2 cut(s) 1036, 1211
Ppu21I YACGTR 2 cut(s) 265, 752
PscI ACATGT 1 cut(s) 767
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 924, 1069
PspPI GGNCC 1 cut(s) 1220
PspXI VCTCGAGB 1 cut(s) 323
RsaI GTAC 6 cut(s) 227, 267, 369, 820, 1034, 1251
RsaNI GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
RseI CAYNNNNRTG 1 cut(s) 766
SaqAI TTAA 4 cut(s) 12, 42, 1023, 1161
SatI GCNGC 2 cut(s) 525, 795
Sau3AI GATC 3 cut(s) 103, 970, 1258
Sau96I GGNCC 1 cut(s) 1220
ScaI AGTACT 1 cut(s) 1034
SchI GAGTC 2 cut(s) 1036, 1212
SduI GDGCHC 2 cut(s) 741, 925
SfaNI GCATC 3 cut(s) 679, 815, 1349
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 1220
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 5 cut(s) 193, 303, 323, 386, 512
SmoI CTYRAG 5 cut(s) 193, 303, 323, 386, 512
SnaBI TACGTA 1 cut(s) 265
SphI GCATGC 1 cut(s) 851
Sse9I AATT 4 cut(s) 1019, 1155, 1266, 1325
SsiI CCGC 5 cut(s) 281, 705, 851, 878, 1121
SspMI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
TaaI ACNGT 4 cut(s) 22, 157, 314, 981
TaiI ACGT 4 cut(s) 129, 267, 669, 754
TaqI TCGA 6 cut(s) 102, 276, 324, 340, 441, 648
TasI AATT 4 cut(s) 1019, 1155, 1266, 1325
TatI WGTACW 2 cut(s) 225, 1032
TfiI GAWTC 3 cut(s) 398, 1007, 1319
Tru1I TTAA 4 cut(s) 12, 42, 1023, 1161
Tru9I TTAA 4 cut(s) 12, 42, 1023, 1161
TseFI GTSAC 3 cut(s) 983, 1076, 1279
TseI GCWGC 2 cut(s) 524, 794
Tsp45I GTSAC 3 cut(s) 983, 1076, 1279
TspDTI ATGAA 5 cut(s) 887, 990, 992, 1020, 1032
VpaK11BI GGWCC 1 cut(s) 1220
XapI RAATTY 2 cut(s) 1019, 1155
XceI RCATGY 4 cut(s) 426, 771, 851, 1000
XhoI CTCGAG 1 cut(s) 323
XspI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
ZrmI AGTACT 1 cut(s) 1034
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.