Rroxscaffold_4G00302690

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017768
Physical Location & Seq
Reverse (-)
23015322 .. 23050032
34711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302690.1

Sequence Viewer

Length: 954 bp
ATGATTTTCTTCTACCCCATTACTGTCCCCAGCTTCAATCTCCCTCAAGCCCTAATCCACTTGAGAAACTCCCTCTCCGAGGCCCTCACTCTGTACTACCCATTGTCTGGAAGGATCAAAAACAACCTCTATGTACATGACTTCGACGCGGGTGTTCCCTACCTTGAAGCTCAAGTCAATTGTCCCATTCTCGAGTTTCTTAAACTTCGAGAAACCGAATGGCTAAACCACCTCGTACCATTCCATCCATTTCTCAAGGAAATGGATTCTGAGCTACTGCCCCTCATCGCATTTCAAGCCAACATCTTCGATGGTGGAATAGCAATGGGCATTTCCTTTTCTCACAAGCTCATTGATGGGGAAACAGCCAATGCTTTTCTCAAGACTTGGTCTGCTATTTTTCGTGGCTGTCGCCAAGAAATCATATACCCTAATCTCGTCCAAGCCTCATTGGTTTTTCCATCACGAGATAACTTGCCTAAAAGGTATGTATCCATCATGGAGAGGTTGTGGTTCCACCAGAAAAAGGATTATGTTACAAGGAGATTTCTGTTTGATGCTAAAGCCATATACGCACTTCAAGTCGAAGCAAAGAGCGAGCTCGTGCCCAAACCATCACGTATTGAGGCACTTACATGTTTTCTCTGGAAACATCAAATGGCTGCCTCTCGGGCGTTATCATCAGGTACTTCTCCAAAACTCTCCATAGTAGCGCATGCAGTAAACTTGAGGCCACGAATGAACCCGCAAATGTTGTTGGATAATACCTTTGGGAATATCGTTTTGTTGGCCCCGGTGGTATTAGAAATCTTGGAAGAGACAACAACAAATAGTAGTGATCATGAAATGTGTGACTTGGTGAACTTGCTTCATGAATCCCTCAACCAATTTAACAGTGAGTACTTGGACACTCGAAGGGTAAAGAAGGATTTGGAGCCATTTGTGACTATCTAG

Protein Analysis

317

Amino Acids

36.39

Weight (kDa)

6.56

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 2 - 301 2.3e-42 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AccII CGCG 1 cut(s) 149
AciI CCGC 2 cut(s) 149, 746
AclWI GGATC 1 cut(s) 122
AfaI GTAC 5 cut(s) 95, 135, 237, 688, 902
AfiI CCNNNNNNNGG 3 cut(s) 79, 107, 526
AflIII ACRYGT 1 cut(s) 635
AgsI TTSAA 4 cut(s) 37, 167, 296, 581
AluBI AGCT 5 cut(s) 33, 170, 274, 349, 601
AluI AGCT 5 cut(s) 33, 170, 274, 349, 601
Alw21I GWGCWC 1 cut(s) 603
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 1 cut(s) 122
Ama87I CYCGRG 2 cut(s) 191, 669
AoxI GGCC 3 cut(s) 81, 731, 789
ApeKI GCWGC 1 cut(s) 662
AspLEI GCGC 1 cut(s) 715
AspS9I GGNCC 2 cut(s) 82, 790
AsuC2I CCSGG 1 cut(s) 794
AsuHPI GGTGA 1 cut(s) 871
AvaI CYCGRG 2 cut(s) 191, 669
BaeGI GKGCMC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 603
BauI CACGAG 2 cut(s) 465, 602
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 649
BccI CCATC 6 cut(s) 252, 305, 350, 469, 503, 622
BciVI GTATCC 1 cut(s) 502
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 1 cut(s) 794
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 1 cut(s) 952
BfuI GTATCC 1 cut(s) 502
BglI GCCNNNNNGGC 1 cut(s) 671
BisI GCNGC 1 cut(s) 663
BlsI GCNGC 1 cut(s) 664
BmcAI AGTACT 1 cut(s) 902
Bme1390I CCNGG 1 cut(s) 794
BmeT110I CYCGRG 2 cut(s) 191, 669
BmgT120I GGNCC 2 cut(s) 82, 790
BmiI GGNNCC 3 cut(s) 515, 792, 936
BmrFI CCNGG 1 cut(s) 794
BmsI GCATC 1 cut(s) 547
BpuEI CTTGAG 6 cut(s) 30, 82, 156, 239, 365, 748
BpuMI CCSGG 1 cut(s) 794
BsaAI YACGTR 1 cut(s) 620
BsaJI CCNNGG 2 cut(s) 78, 792
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 494, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 107, 526
Bse3DI GCAATG 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 78, 792
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 3 cut(s) 79, 107, 526
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 1 cut(s) 649
BseYI CCCAGC 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 3 cut(s) 83, 733, 791
BsiHKAI GWGCWC 1 cut(s) 603
BsiHKCI CYCGRG 2 cut(s) 191, 669
BsiSI CCGG 1 cut(s) 794
BslFI GGGAC 2 cut(s) 11, 168
BslI CCNNNNNNNGG 3 cut(s) 79, 107, 526
BsmAI GTCTC 1 cut(s) 812
BsmFI GGGAC 2 cut(s) 11, 168
BsnI GGCC 3 cut(s) 83, 733, 791
BsoBI CYCGRG 2 cut(s) 191, 669
Bsp1286I GDGCHC 2 cut(s) 603, 609
Bsp1407I TGTACA 1 cut(s) 133
Bsp143I GATC 2 cut(s) 114, 838
BspACI CCGC 2 cut(s) 149, 746
BspANI GGCC 3 cut(s) 83, 733, 791
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 1 cut(s) 149
BspHI TCATGA 2 cut(s) 841, 871
BspLI GGNNCC 3 cut(s) 515, 792, 936
BspPI GGATC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 78, 792
BssMI GATC 2 cut(s) 114, 838
BssSI CACGAG 2 cut(s) 465, 602
Bst2BI CACGAG 2 cut(s) 465, 602
Bst4CI ACNGT 2 cut(s) 25, 896
Bst6I CTCTTC 1 cut(s) 810
BstAUI TGTACA 1 cut(s) 133
BstBAI YACGTR 1 cut(s) 620
BstC8I GCNNGC 2 cut(s) 599, 717
BstDEI CTNAG 1 cut(s) 270
BstENI CCTNNNNNAGG 1 cut(s) 77
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 715
BstKTI GATC 2 cut(s) 117, 841
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 2 cut(s) 114, 838
BstMWI GCNNNNNNNGC 3 cut(s) 296, 572, 671
BstNSI RCATGY 2 cut(s) 639, 719
BstSCI CCNGG 1 cut(s) 792
BstSLI GKGCMC 1 cut(s) 609
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 1 cut(s) 649
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 3 cut(s) 83, 733, 791
BtgZI GCGATG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 901
Cac8I GCNNGC 2 cut(s) 599, 717
CciI TCATGA 2 cut(s) 841, 871
CfoI GCGC 1 cut(s) 715
Cfr13I GGNCC 2 cut(s) 82, 790
CseI GACGC 1 cut(s) 155
Csp6I GTAC 5 cut(s) 94, 134, 236, 687, 901
CviAII CATG 6 cut(s) 137, 499, 636, 716, 842, 872
CviQI GTAC 5 cut(s) 94, 134, 236, 687, 901
DdeI CTNAG 1 cut(s) 270
DpnI GATC 2 cut(s) 116, 840
DpnII GATC 2 cut(s) 114, 838
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
Ecl136II GAGCTC 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 603
Eco53kI GAGCTC 1 cut(s) 601
Eco88I CYCGRG 2 cut(s) 191, 669
EcoICRI GAGCTC 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 603
FaeI CATG 6 cut(s) 140, 502, 639, 719, 845, 875
FaqI GGGAC 2 cut(s) 11, 168
FatI CATG 6 cut(s) 136, 498, 635, 715, 841, 871
FauI CCCGC 2 cut(s) 142, 753
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 1 cut(s) 663
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 1 cut(s) 603
Fsp4HI GCNGC 1 cut(s) 663
FspBI CTAG 1 cut(s) 952
GlaI GCGC 1 cut(s) 714
GluI GCNGC 1 cut(s) 663
GsaI CCCAGC 1 cut(s) 33
HaeIII GGCC 3 cut(s) 83, 733, 791
HapII CCGG 1 cut(s) 794
HgaI GACGC 1 cut(s) 155
HhaI GCGC 1 cut(s) 715
Hin1II CATG 6 cut(s) 140, 502, 639, 719, 845, 875
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HinfI GANTC 2 cut(s) 266, 875
HpaII CCGG 1 cut(s) 794
HphI GGTGA 1 cut(s) 871
Hpy166II GTNNAC 2 cut(s) 724, 862
Hpy188I TCNGA 2 cut(s) 79, 271
Hpy188III TCNNGA 8 cut(s) 108, 191, 209, 382, 465, 646, 842, 872
Hpy8I GTNNAC 2 cut(s) 724, 862
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 105, 909, 919
HpyCH4III ACNGT 2 cut(s) 25, 896
HpyCH4IV ACGT 1 cut(s) 619
HpyCH4V TGCA 1 cut(s) 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 572, 671
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 1 cut(s) 619
Hsp92II CATG 6 cut(s) 140, 502, 639, 719, 845, 875
HspAI GCGC 1 cut(s) 713
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 2 cut(s) 114, 838
LmnI GCTCC 1 cut(s) 934
LpnPI CCDG 6 cut(s) 43, 93, 533, 631, 669, 807
Lsp1109I GCAGC 1 cut(s) 649
LweI GCATC 1 cut(s) 547
MaeI CTAG 1 cut(s) 952
MaeII ACGT 1 cut(s) 619
MaeIII GTNAC 3 cut(s) 535, 851, 943
MalI GATC 2 cut(s) 116, 840
MboI GATC 2 cut(s) 114, 838
MboII GAAGA 2 cut(s) 298, 827
MfeI CAATTG 1 cut(s) 178
MhlI GDGCHC 2 cut(s) 603, 609
MluCI AATT 2 cut(s) 178, 887
MmeI TCCRAC 1 cut(s) 738
MseI TTAA 2 cut(s) 201, 891
MslI CAYNNNNRTG 1 cut(s) 634
MspI CCGG 1 cut(s) 794
MspR9I CCNGG 1 cut(s) 794
MunI CAATTG 1 cut(s) 178
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 3 cut(s) 296, 572, 671
NciI CCSGG 1 cut(s) 794
NdeII GATC 2 cut(s) 114, 838
NlaIII CATG 6 cut(s) 140, 502, 639, 719, 845, 875
NlaIV GGNNCC 3 cut(s) 515, 792, 936
NmuCI GTSAC 2 cut(s) 851, 943
NspI RCATGY 2 cut(s) 639, 719
PaeI GCATGC 1 cut(s) 719
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 2 cut(s) 841, 871
PciI ACATGT 1 cut(s) 635
PfeI GAWTC 2 cut(s) 266, 875
PflFI GACNNNGTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 1 cut(s) 664
Ppu21I YACGTR 1 cut(s) 620
PscI ACATGT 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 603
PspFI CCCAGC 1 cut(s) 29
PspN4I GGNNCC 3 cut(s) 515, 792, 936
PspPI GGNCC 2 cut(s) 82, 790
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 5 cut(s) 95, 135, 237, 688, 902
RsaNI GTAC 5 cut(s) 94, 134, 236, 687, 901
RseI CAYNNNNRTG 1 cut(s) 634
SacI GAGCTC 1 cut(s) 603
SaqAI TTAA 2 cut(s) 201, 891
SatI GCNGC 1 cut(s) 663
Sau3AI GATC 2 cut(s) 114, 838
Sau96I GGNCC 2 cut(s) 82, 790
ScaI AGTACT 1 cut(s) 902
ScrFI CCNGG 1 cut(s) 794
SduI GDGCHC 2 cut(s) 603, 609
SfaNI GCATC 1 cut(s) 547
Sfr274I CTCGAG 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 634
SmlI CTYRAG 7 cut(s) 45, 61, 171, 191, 254, 380, 727
SmoI CTYRAG 7 cut(s) 45, 61, 171, 191, 254, 380, 727
SphI GCATGC 1 cut(s) 719
Sse9I AATT 2 cut(s) 178, 887
SsiI CCGC 2 cut(s) 149, 746
SspMI CTAG 1 cut(s) 952
SstI GAGCTC 1 cut(s) 603
StyD4I CCNGG 1 cut(s) 792
TaaI ACNGT 2 cut(s) 25, 896
TaiI ACGT 1 cut(s) 622
TaqI TCGA 6 cut(s) 144, 192, 208, 309, 585, 913
TasI AATT 2 cut(s) 178, 887
TatI WGTACW 3 cut(s) 93, 133, 900
TfiI GAWTC 2 cut(s) 266, 875
Tru1I TTAA 2 cut(s) 201, 891
Tru9I TTAA 2 cut(s) 201, 891
TscAI CASTG 1 cut(s) 901
TseFI GTSAC 2 cut(s) 851, 943
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 2 cut(s) 851, 943
TspDTI ATGAA 4 cut(s) 755, 858, 860, 888
TspRI CASTG 1 cut(s) 901
Tth111I GACNNNGTC 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 107
XagI CCTNNNNNAGG 1 cut(s) 77
XceI RCATGY 2 cut(s) 639, 719
XhoI CTCGAG 1 cut(s) 191
XspI CTAG 1 cut(s) 952
ZrmI AGTACT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.