Rroxscaffold_4G00302740

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017768
Physical Location & Seq
Reverse (-)
23096514 .. 23103530
7017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302740.1

Sequence Viewer

Length: 1356 bp
ATGGTGAACGTTGAGGTCACTGTTATTTCCAGATACACCATTAAACCATCAACTGCTTCCTCTTCGCCTCACCTTCTACAACCTTACAAGCTTTCCATCTTCGATCAGCTCACTCCCAACACTTATGTCCCAATGATTTTCTTCTACCGCATTACTGTCCCCAGCTTCAATCTCCCTCAAACCCTAATCCACTTGAGAAGCTCCCTCTCCGAGACCCTCACTCTGTACTACCCATTGTCCGGAAGGATAAAAAACAACCTCTACGTACATGACTTTGACGTGGGTGTCCCCTACCTTGAAGCTCAAGTCAACTGTCCCATGCTCGAGTTTCTTAAGCTTCGAGAAATCGAATGGCTAAACCACTTCGTACCATTCCATCCATTTCTCAAGGAAATGGATTCTGAGCTACTCCCCCTCATCGCATGTCAAGCCAACATCTTCGATGGTGGAATAGCAATGGGCATTTCCCTTTCTCACAAGCTCTCTGATGGGGTAACAGCCAATGCTTTTCTCAAGACTTGGGCTGCTATTTTTCGTGGCTGTCGCCAAGAAATCATATACCCTAATCTCTTCCAAGCCTCATTGGTTTTTCCACCACGAGATAACTTGCCTAAAAGGTATGTAGCTCTCATGGAGAGGTTGTGGTTCGGGCAGAAAAAGGATTACGTTACAAGGAGATTTCTGTTTGATGCTAAAGCCATATCCGCACTTCAAGTCAAAGCAAATAGCGAACTCGTGCCCAAACCATCACGTATTGAGGCACTTACATGTTTTCTCTGGAAACATCAAATGGCTGCCTCTCGGGCATCGTCATCAGGTACTTCTTCAAAACTCTCCATAGTAGCGCATGCGGTAAACTTGAGGCCACGAATGAACCCGCAAATGTTGTTGGATAATACCATTGGGAATATCTTTTTGTGGGCTCCGGTGTTATTAGATATCTTGGAAGAGACAGCAACAAAGAGTAGTGATCATGAACTGTGTGACTTGGTGAACATGCTTCATGAATCCCTCAACGAATTTAACAATGAGTACTTGGAGACTCTAAAGGGTAAAGAAGGATTTGGAGCCATTTGTGACTTACTAGATTTGATGGAGGAAGGGACTTCTATAAAACCACCGCCAGAGATTTATGCATTCACAAGCTGGACTAGAATTCTTAACAAACTAGATTTTGGATGGGGAAAGCCATTTCAGATTGGAGTCATCGGAAAAGTTGGACCTATATTTAGAAATCTAACGATATTCGTACAATGCAATCAAGAAATTGAAGCATGGGTGACTTTGGAGGAAAAACAAATGGCTATGCTAGAAAAAGATTCTCAATTTCTAGCGTTTGCATCTCCAAATCCCTAA

Protein Analysis

451

Amino Acids

51.28

Weight (kDa)

6.39

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 443 9.9e-66 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 5 cut(s) 148, 705, 851, 878, 1121
AclI AACGTT 1 cut(s) 9
AcsI RAATTY 2 cut(s) 1019, 1155
AfaI GTAC 6 cut(s) 227, 267, 369, 820, 1034, 1251
AfiI CCNNNNNNNGG 1 cut(s) 239
AflII CTTAAG 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 5 cut(s) 169, 299, 713, 828, 1271
AjiI CACGTC 1 cut(s) 280
Alw26I GTCTC 3 cut(s) 206, 944, 1034
Ama87I CYCGRG 2 cut(s) 323, 801
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 1 cut(s) 863
ApeKI GCWGC 2 cut(s) 524, 794
ApoI RAATTY 2 cut(s) 1019, 1155
AspLEI GCGC 1 cut(s) 847
AspS9I GGNCC 1 cut(s) 1220
AsuHPI GGTGA 4 cut(s) 16, 62, 1003, 1291
AvaI CYCGRG 2 cut(s) 323, 801
AvaII GGWCC 1 cut(s) 1220
BaeGI GKGCMC 1 cut(s) 741
BanII GRGCYC 1 cut(s) 925
BauI CACGAG 2 cut(s) 597, 734
BbvI GCAGC 2 cut(s) 511, 781
BccI CCATC 8 cut(s) 55, 104, 384, 437, 482, 754, 1087, 1173
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 3 cut(s) 206, 944, 1034
BfaI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
BfrI CTTAAG 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 803
BisI GCNGC 2 cut(s) 525, 795
BlsI GCNGC 2 cut(s) 526, 796
BmcAI AGTACT 1 cut(s) 1034
Bme18I GGWCC 1 cut(s) 1220
BmeT110I CYCGRG 2 cut(s) 323, 801
BmgBI CACGTC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 1220
BmiI GGNNCC 2 cut(s) 924, 1069
BmsI GCATC 3 cut(s) 679, 815, 1349
BpuEI CTTGAG 5 cut(s) 214, 288, 371, 497, 880
BsaAI YACGTR 2 cut(s) 265, 752
BsaI GGTCTC 1 cut(s) 206
BsaWI WCCGGW 2 cut(s) 239, 925
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 239
BseGI GGATG 2 cut(s) 376, 1184
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 1 cut(s) 393
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 2 cut(s) 511, 781
BseYI CCCAGC 1 cut(s) 161
BshFI GGCC 1 cut(s) 865
BsiHKCI CYCGRG 2 cut(s) 323, 801
BsiSI CCGG 2 cut(s) 240, 926
BslFI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 3 cut(s) 206, 944, 1034
BsmFI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
BsmI GAATGC 1 cut(s) 1136
BsnI GGCC 1 cut(s) 865
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 2 cut(s) 323, 801
Bsp1286I GDGCHC 2 cut(s) 741, 925
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 2 cut(s) 103, 970
BspACI CCGC 5 cut(s) 148, 705, 851, 878, 1121
BspANI GGCC 1 cut(s) 865
BspCNI CTCAG 1 cut(s) 394
BspEI TCCGGA 1 cut(s) 239
BspHI TCATGA 2 cut(s) 973, 1003
BspLI GGNNCC 2 cut(s) 924, 1069
BspTI CTTAAG 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 462
BssMI GATC 2 cut(s) 103, 970
BssSI CACGAG 2 cut(s) 597, 734
Bst2BI CACGAG 2 cut(s) 597, 734
Bst4CI ACNGT 4 cut(s) 22, 157, 314, 981
Bst6I CTCTTC 3 cut(s) 67, 575, 942
BstAFI CTTAAG 1 cut(s) 332
BstBAI YACGTR 2 cut(s) 265, 752
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 376, 1184
BstHHI GCGC 1 cut(s) 847
BstKTI GATC 2 cut(s) 106, 973
BstMAI GTCTC 3 cut(s) 206, 944, 1034
BstMBI GATC 2 cut(s) 103, 970
BstMWI GCNNNNNNNGC 3 cut(s) 428, 704, 803
BstNSI RCATGY 4 cut(s) 426, 771, 851, 1000
BstSLI GKGCMC 1 cut(s) 741
BstSNI TACGTA 1 cut(s) 265
BstV1I GCAGC 2 cut(s) 511, 781
BsuRI GGCC 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 403
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 2 cut(s) 376, 1184
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 849
CciI TCATGA 2 cut(s) 973, 1003
CfoI GCGC 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 1220
Csp6I GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
CviQI GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
DdeI CTNAG 1 cut(s) 402
DpnI GATC 2 cut(s) 105, 972
DpnII GATC 2 cut(s) 103, 970
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 67, 575, 942
EarI CTCTTC 3 cut(s) 67, 575, 942
Eco105I TACGTA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 925
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 940
Eco47I GGWCC 1 cut(s) 1220
Eco88I CYCGRG 2 cut(s) 323, 801
EcoRI GAATTC 1 cut(s) 1155
EcoRV GATATC 1 cut(s) 940
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 925
FaqI GGGAC 5 cut(s) 113, 143, 272, 300, 1117
FauI CCCGC 1 cut(s) 885
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 2 cut(s) 525, 795
FokI GGATG 2 cut(s) 363, 1191
FriOI GRGCYC 1 cut(s) 925
Fsp4HI GCNGC 2 cut(s) 525, 795
FspBI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
GlaI GCGC 1 cut(s) 846
GluI GCNGC 2 cut(s) 525, 795
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 1 cut(s) 865
HapII CCGG 2 cut(s) 240, 926
HhaI GCGC 1 cut(s) 847
Hin6I GCGC 1 cut(s) 845
HinP1I GCGC 1 cut(s) 845
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 2 cut(s) 89, 335
HinfI GANTC 5 cut(s) 398, 1007, 1042, 1203, 1319
HpaII CCGG 2 cut(s) 240, 926
HphI GGTGA 4 cut(s) 16, 62, 1003, 1291
Hpy166II GTNNAC 4 cut(s) 7, 310, 856, 994
Hpy188I TCNGA 5 cut(s) 211, 403, 487, 1197, 1211
Hpy188III TCNNGA 8 cut(s) 30, 240, 341, 514, 778, 974, 1004, 1262
Hpy8I GTNNAC 4 cut(s) 7, 310, 856, 994
HpyAV CCTTC 4 cut(s) 83, 237, 1052, 1094
HpyCH4III ACNGT 4 cut(s) 22, 157, 314, 981
HpyCH4IV ACGT 5 cut(s) 9, 264, 279, 666, 751
HpyCH4V TGCA 3 cut(s) 1136, 1257, 1340
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 704, 803
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 5 cut(s) 9, 264, 279, 666, 751
HspAI GCGC 1 cut(s) 845
Kpn2I TCCGGA 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 2 cut(s) 103, 970
LmnI GCTCC 3 cut(s) 206, 928, 1067
LpnPI CCDG 8 cut(s) 43, 175, 253, 763, 801, 939, 1132, 1137
Lsp1109I GCAGC 2 cut(s) 511, 781
LweI GCATC 3 cut(s) 679, 815, 1349
MaeI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
MaeII ACGT 5 cut(s) 9, 264, 279, 666, 751
MaeIII GTNAC 6 cut(s) 16, 493, 667, 983, 1076, 1279
MalI GATC 2 cut(s) 105, 972
MboI GATC 2 cut(s) 103, 970
MboII GAAGA 7 cut(s) 54, 91, 133, 430, 562, 816, 959
MhlI GDGCHC 2 cut(s) 741, 925
MluCI AATT 4 cut(s) 1019, 1155, 1266, 1325
MlyI GAGTC 2 cut(s) 1036, 1212
MmeI TCCRAC 2 cut(s) 870, 1198
Mph1103I ATGCAT 1 cut(s) 1138
MroI TCCGGA 1 cut(s) 239
MseI TTAA 4 cut(s) 42, 333, 1023, 1161
MslI CAYNNNNRTG 1 cut(s) 766
MspCI CTTAAG 1 cut(s) 332
MspI CCGG 2 cut(s) 240, 926
Mva1269I GAATGC 1 cut(s) 1136
MwoI GCNNNNNNNGC 3 cut(s) 428, 704, 803
NdeII GATC 2 cut(s) 103, 970
NlaIV GGNNCC 2 cut(s) 924, 1069
NmuCI GTSAC 4 cut(s) 16, 983, 1076, 1279
NsiI ATGCAT 1 cut(s) 1138
NspI RCATGY 4 cut(s) 426, 771, 851, 1000
PaeI GCATGC 1 cut(s) 851
PaeR7I CTCGAG 1 cut(s) 323
PagI TCATGA 2 cut(s) 973, 1003
PciI ACATGT 1 cut(s) 767
PctI GAATGC 1 cut(s) 1136
PfeI GAWTC 3 cut(s) 398, 1007, 1319
PkrI GCNGC 2 cut(s) 526, 796
PleI GAGTC 2 cut(s) 1036, 1211
PpsI GAGTC 2 cut(s) 1036, 1211
Ppu21I YACGTR 2 cut(s) 265, 752
PscI ACATGT 1 cut(s) 767
Psp1406I AACGTT 1 cut(s) 9
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 924, 1069
PspPI GGNCC 1 cut(s) 1220
PspXI VCTCGAGB 1 cut(s) 323
RsaI GTAC 6 cut(s) 227, 267, 369, 820, 1034, 1251
RsaNI GTAC 6 cut(s) 226, 266, 368, 819, 1033, 1250
RseI CAYNNNNRTG 1 cut(s) 766
SaqAI TTAA 4 cut(s) 42, 333, 1023, 1161
SatI GCNGC 2 cut(s) 525, 795
Sau3AI GATC 2 cut(s) 103, 970
Sau96I GGNCC 1 cut(s) 1220
ScaI AGTACT 1 cut(s) 1034
SchI GAGTC 2 cut(s) 1036, 1212
SduI GDGCHC 2 cut(s) 741, 925
SfaNI GCATC 3 cut(s) 679, 815, 1349
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 1220
SlaI CTCGAG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 766
SmlI CTYRAG 7 cut(s) 193, 303, 323, 332, 386, 512, 859
SmoI CTYRAG 7 cut(s) 193, 303, 323, 332, 386, 512, 859
SnaBI TACGTA 1 cut(s) 265
SphI GCATGC 1 cut(s) 851
Sse9I AATT 4 cut(s) 1019, 1155, 1266, 1325
SsiI CCGC 5 cut(s) 148, 705, 851, 878, 1121
SspMI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
TaaI ACNGT 4 cut(s) 22, 157, 314, 981
TaiI ACGT 5 cut(s) 12, 267, 282, 669, 754
TaqI TCGA 5 cut(s) 102, 324, 340, 348, 441
TasI AATT 4 cut(s) 1019, 1155, 1266, 1325
TatI WGTACW 2 cut(s) 225, 1032
TfiI GAWTC 3 cut(s) 398, 1007, 1319
Tru1I TTAA 4 cut(s) 42, 333, 1023, 1161
Tru9I TTAA 4 cut(s) 42, 333, 1023, 1161
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 4 cut(s) 16, 983, 1076, 1279
TseI GCWGC 2 cut(s) 524, 794
Tsp45I GTSAC 4 cut(s) 16, 983, 1076, 1279
TspDTI ATGAA 4 cut(s) 887, 990, 992, 1020
TspRI CASTG 1 cut(s) 25
Vha464I CTTAAG 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 1220
XapI RAATTY 2 cut(s) 1019, 1155
XceI RCATGY 4 cut(s) 426, 771, 851, 1000
XhoI CTCGAG 1 cut(s) 323
XspI CTAG 5 cut(s) 1085, 1152, 1169, 1310, 1331
ZrmI AGTACT 1 cut(s) 1034
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.