RLG00000028777

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018652
Physical Location & Seq
Reverse (-)
27820765 .. 27823997
3233 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028777

Sequence Viewer

Length: 699 bp
ATGAAACTGCTATGGGAACTTTGTCGACAGCCGTCAGGTGGGTGGATAAAGATGGTCGACGATGGCAGAGCGGAGATTGGAAAGGTCGGAGAGGTCGATGGTGGGGATTGGTTGGGCGTCGATGATGGTAGAGCAACGATTAGAGTGGAGGTTAGAGAGGTCGATGGTGGGGATTGGTTGGACGTCGGGTCGGGTCGTCGCTGGTGGTTTTGCGTCGTCGAAGAGGCTGATCGGAGCAGCAACGACGACTGGTGGTTCTACAAAATGATCAGAGCGGCTGGTGGTTCTGCAAAACGATCGGAGCAGAGGTCGGGAGTGTCGTTGACGAGGCCGAGAGCAACTCGAGAATCTCCTCCAGGTTATCCAGTAATTTTCCGGCCATTCTGCTACAACGTGTTCCTCAATTTCAGAGGCGAAGACACCTACAAGACTTTCACCGACCACCTCTACACTGCCCTGGTCAACGCCGGAATCCGCACTTTCCGAGAAGACTACGAAATCGAGAGAGGACAAGATATCAAGCAAGAGCTGAACGAAGCCATCCACCGATCATGCAGCTCCGTCGTCGTATTTTCTGAAGACTACGCCTCTTCCCGATGGCTCCTCGACGAGCTTGTCGTCATCCTCCAACGCCACGCCGACGGCGCTCGCGGTCATGTCATTTTGCCAGTATTCTACGACATCGATCTAGTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

233

Amino Acids

26.59

Weight (kDa)

5.24

Isoelectric Point (pI)

61.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 130 - 229 2.3e-28 TIR domain
TIR_2 PF13676 132 - 227 2.4e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018652)

Species Orthologous Gene IDs
rosa_laevigata RLG00000007863 RLG00000028777
rosa_roxburghii Rroxscaffold_7G00177670 Rroxscaffold_7G00178150
rosa_rugosa Rorug05G0552600
rosa_samantha Rh1DG169200 Rh2DG630400 Rh6BG090300 Rh7CG411700 Rh7DG011600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AatII GACGTC 1 cut(s) 186
AccBSI CCGCTC 2 cut(s) 71, 275
AccI GTMKAC 2 cut(s) 25, 57
AccII CGCG 1 cut(s) 651
AciI CCGC 4 cut(s) 71, 275, 475, 651
AcoI YGGCCR 1 cut(s) 377
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 2 cut(s) 117, 183
AfiI CCNNNNNNNGG 1 cut(s) 38
AflIII ACRYGT 1 cut(s) 393
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 2 cut(s) 355, 456
AluBI AGCT 3 cut(s) 529, 558, 613
AluI AGCT 3 cut(s) 529, 558, 613
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 2 cut(s) 329, 377
ApeKI GCWGC 2 cut(s) 237, 555
AspLEI GCGC 1 cut(s) 647
AsuHPI GGTGA 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 342
BbsI GAAGAC 3 cut(s) 423, 495, 585
BbvI GCAGC 2 cut(s) 249, 567
BccI CCATC 7 cut(s) 46, 56, 92, 119, 158, 548, 591
BceAI ACGGC 2 cut(s) 16, 658
BcgI CGANNNNNNTGC 4 cut(s) 279, 313, 544, 578
BciT130I CCWGG 2 cut(s) 357, 458
BclI TGATCA 1 cut(s) 267
BfaI CTAG 1 cut(s) 689
BfoI RGCGCY 1 cut(s) 648
BisI GCNGC 3 cut(s) 238, 276, 556
BlsI GCNGC 3 cut(s) 239, 277, 557
Bme1390I CCNGG 2 cut(s) 357, 458
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 342
BmiI GGNNCC 1 cut(s) 602
BmrFI CCNGG 2 cut(s) 357, 458
BoxI GACNNNNGTC 1 cut(s) 31
BpiI GAAGAC 3 cut(s) 423, 495, 585
BplI GAGNNNNNCTC 2 cut(s) 325, 357
BpmI CTGGAG 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 684
BsaHI GRCGYC 2 cut(s) 117, 183
BsaJI CCNNGG 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse1I ACTGG 3 cut(s) 254, 365, 668
BseBI CCWGG 2 cut(s) 357, 458
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 2 cut(s) 540, 621
BseLI CCNNNNNNNGG 1 cut(s) 38
BseNI ACTGG 3 cut(s) 254, 365, 668
BseRI GAGGAG 2 cut(s) 342, 593
BseXI GCAGC 2 cut(s) 249, 567
Bsh1236I CGCG 1 cut(s) 651
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 2 cut(s) 331, 379
BshVI ATCGAT 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 342
BsiSI CCGG 2 cut(s) 376, 468
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 2 cut(s) 331, 379
BsoBI CYCGRG 1 cut(s) 342
Bsp143I GATC 5 cut(s) 229, 267, 296, 548, 685
BspACI CCGC 4 cut(s) 71, 275, 475, 651
BspANI GGCC 2 cut(s) 331, 379
BspDI ATCGAT 1 cut(s) 684
BspFNI CGCG 1 cut(s) 651
BspLI GGNNCC 1 cut(s) 602
BsrBI CCGCTC 2 cut(s) 71, 275
BsrI ACTGG 3 cut(s) 254, 365, 668
BssECI CCNNGG 1 cut(s) 456
BssMI GATC 5 cut(s) 229, 267, 296, 548, 685
BssNI GRCGYC 2 cut(s) 117, 183
Bst2UI CCWGG 2 cut(s) 357, 458
Bst6I CTCTTC 2 cut(s) 216, 595
BstACI GRCGYC 2 cut(s) 117, 183
BstC8I GCNNGC 1 cut(s) 649
BstF5I GGATG 2 cut(s) 540, 621
BstFNI CGCG 1 cut(s) 651
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 1 cut(s) 647
BstKTI GATC 5 cut(s) 232, 270, 299, 551, 688
BstMBI GATC 5 cut(s) 229, 267, 296, 548, 685
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 1 cut(s) 644
BstNI CCWGG 2 cut(s) 357, 458
BstPAI GACNNNNGTC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 355, 456
BstUI CGCG 1 cut(s) 651
BstV1I GCAGC 2 cut(s) 249, 567
BstV2I GAAGAC 3 cut(s) 423, 495, 585
Bsu15I ATCGAT 1 cut(s) 684
BsuRI GGCC 2 cut(s) 331, 379
BsuTUI ATCGAT 1 cut(s) 684
BtsCI GGATG 2 cut(s) 540, 621
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 1 cut(s) 649
CfoI GCGC 1 cut(s) 647
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 2 cut(s) 106, 202
CviAII CATG 2 cut(s) 552, 656
DpnI GATC 5 cut(s) 231, 269, 298, 550, 687
DpnII GATC 5 cut(s) 229, 267, 296, 548, 685
DrdI GACNNNNNNGTC 1 cut(s) 614
DriI GACNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 1 cut(s) 377
Eam1104I CTCTTC 2 cut(s) 216, 595
Eam1105I GACNNNNNGTC 1 cut(s) 187
EarI CTCTTC 2 cut(s) 216, 595
Eco32I GATATC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 342
EcoRII CCWGG 2 cut(s) 355, 456
EcoRV GATATC 1 cut(s) 517
FaeI CATG 2 cut(s) 555, 659
FaiI YATR 3 cut(s) 13, 553, 657
FatI CATG 2 cut(s) 551, 655
FbaI TGATCA 1 cut(s) 267
FblI GTMKAC 2 cut(s) 25, 57
Fnu4HI GCNGC 3 cut(s) 238, 276, 556
FokI GGATG 2 cut(s) 527, 608
Fsp4HI GCNGC 3 cut(s) 238, 276, 556
FspBI CTAG 1 cut(s) 689
GlaI GCGC 1 cut(s) 646
GluI GCNGC 3 cut(s) 238, 276, 556
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 648
HaeIII GGCC 2 cut(s) 331, 379
HapII CCGG 2 cut(s) 376, 468
HgaI GACGC 2 cut(s) 106, 202
HhaI GCGC 1 cut(s) 647
Hin1I GRCGYC 2 cut(s) 117, 183
Hin1II CATG 2 cut(s) 555, 659
Hin6I GCGC 1 cut(s) 645
HinP1I GCGC 1 cut(s) 645
HincII GTYRAC 4 cut(s) 26, 58, 324, 463
HindII GTYRAC 4 cut(s) 26, 58, 324, 463
HinfI GANTC 2 cut(s) 347, 471
HpaII CCGG 2 cut(s) 376, 468
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 4 cut(s) 26, 58, 324, 463
Hpy188I TCNGA 7 cut(s) 89, 234, 272, 301, 410, 485, 577
Hpy188III TCNNGA 4 cut(s) 312, 344, 502, 594
Hpy8I GTNNAC 4 cut(s) 26, 58, 324, 463
HpyCH4IV ACGT 2 cut(s) 183, 393
HpyCH4V TGCA 2 cut(s) 290, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 644
HpySE526I ACGT 2 cut(s) 183, 393
Hsp92I GRCGYC 2 cut(s) 117, 183
Hsp92II CATG 2 cut(s) 555, 659
HspAI GCGC 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 5 cut(s) 229, 267, 296, 548, 685
LmnI GCTCC 4 cut(s) 234, 301, 563, 606
Lsp1109I GCAGC 2 cut(s) 249, 567
MaeI CTAG 1 cut(s) 689
MaeII ACGT 2 cut(s) 183, 393
MalI GATC 5 cut(s) 231, 269, 298, 550, 687
MbiI CCGCTC 2 cut(s) 71, 275
MboI GATC 5 cut(s) 229, 267, 296, 548, 685
MboII GAAGA 5 cut(s) 233, 428, 500, 582, 590
MluCI AATT 2 cut(s) 369, 403
MmeI TCCRAC 3 cut(s) 67, 159, 652
MspI CCGG 2 cut(s) 376, 468
MspR9I CCNGG 2 cut(s) 357, 458
MvaI CCWGG 2 cut(s) 357, 458
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 1 cut(s) 644
NdeII GATC 5 cut(s) 229, 267, 296, 548, 685
NlaIII CATG 2 cut(s) 555, 659
NlaIV GGNNCC 1 cut(s) 602
NmeAIII GCCGAG 1 cut(s) 357
PaeR7I CTCGAG 1 cut(s) 342
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 317, 615
PfeI GAWTC 2 cut(s) 347, 471
PkrI GCNGC 3 cut(s) 239, 277, 557
Ple19I CGATCG 1 cut(s) 299
PshAI GACNNNNGTC 1 cut(s) 31
Psp6I CCWGG 2 cut(s) 355, 456
PspGI CCWGG 2 cut(s) 355, 456
PspN4I GGNNCC 1 cut(s) 602
PvuI CGATCG 1 cut(s) 299
SalI GTCGAC 2 cut(s) 24, 56
SatI GCNGC 3 cut(s) 238, 276, 556
Sau3AI GATC 5 cut(s) 229, 267, 296, 548, 685
ScrFI CCNGG 2 cut(s) 357, 458
Sfr274I CTCGAG 1 cut(s) 342
SlaI CTCGAG 1 cut(s) 342
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 2 cut(s) 369, 403
SsiI CCGC 4 cut(s) 71, 275, 475, 651
SspMI CTAG 1 cut(s) 689
StyD4I CCNGG 2 cut(s) 355, 456
TaiI ACGT 2 cut(s) 186, 396
TasI AATT 2 cut(s) 369, 403
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 347, 471
TscAI CASTG 1 cut(s) 457
TseI GCWGC 2 cut(s) 237, 555
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 342
XmiI GTMKAC 2 cut(s) 25, 57
XspI CTAG 1 cut(s) 689
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.