RLG00000031470

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017097
Physical Location & Seq
Reverse (-)
5477294 .. 5478004
711 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031470

Sequence Viewer

Length: 615 bp
ATGCAGCGTTCATCACCAGCTTTGGTTCGTCAGATACTCCGCCGCAACCGCATGAGCAACGTCCAAATGGTACCGACCCTCTCTTCGACGCCGCCGCCGCCGCCTCCCGGCCCACCCTGCGACGTGTTCATAAACCACCGCGGGATCGACACCAAGAGAACCGTGGCGGGGCTGCTGAACTACCACCTGTCCAGCAGGCTCAACCTGCGTCCTTTTTTGGACAGCAAGAACATGAAGCCAGGGGACAGGTTGTTTGATAAGATCGACGCCGCGATTCGGAAGTGCAAGCTTGGGATCGCCGTGTTTTCACCGCTGTACTGCGAATCTTACTTCTGTCTCCACGAACTCGCTCTTCTAATGGAATCTAAAAAGAGGGTTATACCGGTTTTCTGCAACGTGAAGCCGTCTCAGCTTAGGGTTAAGGATAATGGAGTTTGTCCTCAGTACGAGATCGAGAGGTTTAGTTTGGCGCTTGAAGAAGCCAAATACACAGTTGGACTTACCTTCGACTCCTCTAAAGCCGATTGGTCGGAGTTTCTAAGAGAGGCTTCGGATGCGGTGATGCAGAACTTGCTGGAGGTAGAAGGAGAGGCATATCTACAGGAGAAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR TIR_2

Protein Analysis

205

Amino Acids

23.15

Weight (kDa)

8.83

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 41 - 140 8.5e-15 TIR domain
TIR_2 PF13676 42 - 135 7.7e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61105
fragaria_vesca FvH4_3g04640
malus_domestica MD10G1299700.v1.1
prunus_persica Prupe.4G041100_v2.0.a1
rosa_laevigata RLG00000031470
rosa_multiflora Rmu_co8469441.1_g000001 Rmu_ssc0000182.1_g000021
rosa_rugosa Rorug04G0428700
rosa_samantha Rh5AG056600 Rh5BG054000 Rh5CG063700 Rh5DG053000
rosa_wichuraiana Rw5G005380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 213
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 70
AccII CGCG 2 cut(s) 141, 272
AclWI GGATC 2 cut(s) 152, 302
AcyI GRCGYC 2 cut(s) 89, 267
AfaI GTAC 3 cut(s) 72, 317, 446
AfiI CCNNNNNNNGG 3 cut(s) 107, 168, 276
AflIII ACRYGT 1 cut(s) 123
AgeI ACCGGT 1 cut(s) 382
AgsI TTSAA 1 cut(s) 476
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 3 cut(s) 20, 289, 412
AluI AGCT 3 cut(s) 20, 289, 412
Alw26I GTCTC 2 cut(s) 341, 411
AlwI GGATC 2 cut(s) 152, 302
AoxI GGCC 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 4, 172
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 236, 268
AsiGI ACCGGT 1 cut(s) 382
Asp718I GGTACC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 3 cut(s) 6, 300, 571
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 16, 159
BceAI ACGGC 2 cut(s) 284, 388
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 341, 411
BfaI CTAG 1 cut(s) 613
BfmI CTRYAG 1 cut(s) 599
BfoI RGCGCY 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 213
BisI GCNGC 8 cut(s) 5, 43, 92, 95, 98, 101, 173, 270
BlsI GCNGC 8 cut(s) 6, 44, 93, 96, 99, 102, 174, 271
Bme1390I CCNGG 2 cut(s) 108, 240
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 108, 240
BmsI GCATC 2 cut(s) 544, 552
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 413
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 89, 267
BsaJI CCNNGG 3 cut(s) 139, 162, 239
BsaWI WCCGGW 1 cut(s) 382
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 168, 276
Bse118I RCCGGY 1 cut(s) 382
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 139, 162, 239
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 3 cut(s) 107, 168, 276
BseMII CTCAG 2 cut(s) 422, 455
BseRI GAGGAG 1 cut(s) 502
BseXI GCAGC 2 cut(s) 16, 159
Bsh1236I CGCG 2 cut(s) 141, 272
BshFI GGCC 1 cut(s) 111
BshNI GGYRCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 382
BsiSI CCGG 2 cut(s) 108, 383
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 3 cut(s) 107, 168, 276
BsmAI GTCTC 2 cut(s) 341, 411
BsmBI CGTCTC 1 cut(s) 411
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 1 cut(s) 111
Bsp143I GATC 4 cut(s) 144, 261, 294, 450
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 2 cut(s) 421, 454
BspFNI CGCG 2 cut(s) 141, 272
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 2 cut(s) 152, 302
BspQI GCTCTTC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 382
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 3 cut(s) 139, 162, 239
BssMI GATC 4 cut(s) 144, 261, 294, 450
BssNI GRCGYC 2 cut(s) 89, 267
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 2 cut(s) 163, 493
Bst6I CTCTTC 2 cut(s) 88, 357
BstACI GRCGYC 2 cut(s) 89, 267
BstAPI GCANNNNNTGC 1 cut(s) 571
BstC8I GCNNGC 2 cut(s) 197, 287
BstDEI CTNAG 4 cut(s) 408, 413, 441, 539
BstDSI CCRYGG 2 cut(s) 139, 162
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 2 cut(s) 141, 272
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 4 cut(s) 147, 264, 297, 453
BstMAI GTCTC 2 cut(s) 341, 411
BstMBI GATC 4 cut(s) 144, 261, 294, 450
BstMWI GCNNNNNNNGC 8 cut(s) 48, 97, 100, 117, 205, 409, 554, 571
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 2 cut(s) 106, 238
BstSFI CTRYAG 1 cut(s) 599
BstUI CGCG 2 cut(s) 141, 272
BstV1I GCAGC 2 cut(s) 16, 159
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 2 cut(s) 139, 162
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 1 cut(s) 559
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 197, 287
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 110
Cfr42I CCGCGG 1 cut(s) 142
CseI GACGC 3 cut(s) 97, 197, 275
Csp6I GTAC 3 cut(s) 71, 316, 445
CspAI ACCGGT 1 cut(s) 382
CviAII CATG 2 cut(s) 52, 232
CviQI GTAC 3 cut(s) 71, 316, 445
DdeI CTNAG 4 cut(s) 408, 413, 441, 539
DpnI GATC 4 cut(s) 146, 263, 296, 452
DpnII GATC 4 cut(s) 144, 261, 294, 450
Eam1104I CTCTTC 2 cut(s) 88, 357
EarI CTCTTC 2 cut(s) 88, 357
EciI GGCGGA 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 411
FaeI CATG 2 cut(s) 55, 235
FaiI YATR 5 cut(s) 53, 131, 233, 380, 595
FalI AAGNNNNNCTT 2 cut(s) 532, 564
FaqI GGGAC 1 cut(s) 257
FatI CATG 2 cut(s) 51, 231
FauI CCCGC 2 cut(s) 134, 160
Fnu4HI GCNGC 8 cut(s) 5, 43, 92, 95, 98, 101, 173, 270
FokI GGATG 1 cut(s) 566
Fsp4HI GCNGC 8 cut(s) 5, 43, 92, 95, 98, 101, 173, 270
FspBI CTAG 1 cut(s) 613
GlaI GCGC 1 cut(s) 471
GluI GCNGC 8 cut(s) 5, 43, 92, 95, 98, 101, 173, 270
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 473
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 108, 383
HgaI GACGC 3 cut(s) 97, 197, 275
HhaI GCGC 1 cut(s) 472
Hin1I GRCGYC 2 cut(s) 89, 267
Hin1II CATG 2 cut(s) 55, 235
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 4 cut(s) 274, 323, 362, 509
HpaII CCGG 2 cut(s) 108, 383
HphI GGTGA 3 cut(s) 6, 300, 571
Hpy188I TCNGA 4 cut(s) 33, 279, 532, 553
Hpy188III TCNNGA 1 cut(s) 454
Hpy99I CGWCG 3 cut(s) 91, 125, 269
HpyAV CCTTC 2 cut(s) 514, 578
HpyCH4III ACNGT 2 cut(s) 163, 493
HpyCH4IV ACGT 3 cut(s) 60, 123, 396
HpyCH4V TGCA 4 cut(s) 4, 285, 393, 565
HpyF10VI GCNNNNNNNGC 8 cut(s) 48, 97, 100, 117, 205, 409, 554, 571
HpyF3I CTNAG 4 cut(s) 408, 413, 441, 539
HpySE526I ACGT 3 cut(s) 60, 123, 396
Hsp92I GRCGYC 2 cut(s) 89, 267
Hsp92II CATG 2 cut(s) 55, 235
HspAI GCGC 1 cut(s) 470
KpnI GGTACC 1 cut(s) 74
KspI CCGCGG 1 cut(s) 142
Kzo9I GATC 4 cut(s) 144, 261, 294, 450
LguI GCTCTTC 1 cut(s) 357
Lsp1109I GCAGC 2 cut(s) 16, 159
LweI GCATC 2 cut(s) 544, 552
MaeI CTAG 1 cut(s) 613
MaeII ACGT 3 cut(s) 60, 123, 396
MalI GATC 4 cut(s) 146, 263, 296, 452
MboI GATC 4 cut(s) 144, 261, 294, 450
MboII GAAGA 3 cut(s) 75, 344, 488
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 2 cut(s) 475, 510
MnlI CCTC 9 cut(s) 89, 114, 366, 450, 450, 523, 538, 571, 583
MseI TTAA 1 cut(s) 420
MspA1I CMGCKG 2 cut(s) 141, 313
MspI CCGG 2 cut(s) 108, 383
MspR9I CCNGG 2 cut(s) 108, 240
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 2 cut(s) 141, 272
MwoI GCNNNNNNNGC 8 cut(s) 48, 97, 100, 117, 205, 409, 554, 571
NciI CCSGG 1 cut(s) 108
NdeII GATC 4 cut(s) 144, 261, 294, 450
NlaIII CATG 2 cut(s) 55, 235
NlaIV GGNNCC 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 357
PfeI GAWTC 3 cut(s) 274, 323, 362
PinAI ACCGGT 1 cut(s) 382
PkrI GCNGC 8 cut(s) 6, 44, 93, 96, 99, 102, 174, 271
PleI GAGTC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 110
RsaI GTAC 3 cut(s) 72, 317, 446
RsaNI GTAC 3 cut(s) 71, 316, 445
SacII CCGCGG 1 cut(s) 142
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 8 cut(s) 5, 43, 92, 95, 98, 101, 173, 270
Sau3AI GATC 4 cut(s) 144, 261, 294, 450
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 108, 240
SfaNI GCATC 2 cut(s) 544, 552
SfcI CTRYAG 1 cut(s) 599
Sfr303I CCGCGG 1 cut(s) 142
SgrBI CCGCGG 1 cut(s) 142
SspMI CTAG 1 cut(s) 613
StyD4I CCNGG 2 cut(s) 106, 238
TaaI ACNGT 2 cut(s) 163, 493
TaiI ACGT 3 cut(s) 63, 126, 399
TaqI TCGA 5 cut(s) 86, 147, 264, 453, 507
TatI WGTACW 1 cut(s) 315
TauI GCSGC 6 cut(s) 45, 94, 97, 100, 103, 272
TfiI GAWTC 3 cut(s) 274, 323, 362
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TseI GCWGC 2 cut(s) 4, 172
TspDTI ATGAA 2 cut(s) 118, 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.