Rh5DG053000

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0017097
Physical Location & Seq
Reverse (-)
4522241 .. 4522939
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG053000.1

Sequence Viewer

Length: 624 bp
ATGCAGCGTTCATCACCAGCTTTGGTTCGTCAGATACTCCACCGCAACCGCATGAGCAACGTCCAAATGGTACCGACCCTCTCTTCGCCGCCGCCGCCGCCGCCTCCCCCCCCCGGCCCACCCTGCGACGTGTTCATAAACCACCGCGGGATCGACACCAAGAGAACCGTGGCGGGGCTGCTGAACTACCACCTGTCCAGCAGGCTCAACCTGCGTCCTTTTTTGGACAGCAAGAACATGAAGCCAGGGGACAGGTTGTTTGATAAGATCGACGCCGCGATTCGGAAATGCAAGCTTGGGATCGCCGTGTTTTCACCGCAGTACTGCGAATCTTACTTCTGTCTCCACGAACTCGCTCTTCTAATGGAATCTAAAAAGAGGGTTATACCGGTTTTCTGCAACGTGAAGCCGTCTCAGCTTAGGGTTAAGGATAATGGAGTTTGCCCTCAGTACGAGATCGAGAGGTTTAGTTTGGCGCTTGAAGAAGCCAAATACACAGTTGGACTTACCTTCGACTCCTCTAAAGCCGATTGGTCGGAGTTTCTAAGAGAGGCTTCGGATGCGGTGATGCAGAACTTGCTGGAGGTAGAAGGAGAACAAGCATATCTACAGGAGAAATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR TIR_2

Protein Analysis

207

Amino Acids

23.46

Weight (kDa)

8.66

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 43 - 142 1.6e-15 TIR domain
TIR_2 PF13676 44 - 137 2.2e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61105
fragaria_vesca FvH4_3g04640
malus_domestica MD10G1299700.v1.1
prunus_persica Prupe.4G041100_v2.0.a1
rosa_laevigata RLG00000031470
rosa_multiflora Rmu_co8469441.1_g000001 Rmu_ssc0000182.1_g000021
rosa_rugosa Rorug04G0428700
rosa_samantha Rh5AG056600 Rh5BG054000 Rh5CG063700 Rh5DG053000
rosa_wichuraiana Rw5G005380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 219
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 70
AccII CGCG 2 cut(s) 147, 278
AclWI GGATC 2 cut(s) 158, 308
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 3 cut(s) 72, 323, 452
AfiI CCNNNNNNNGG 3 cut(s) 113, 174, 282
AflIII ACRYGT 1 cut(s) 129
AgeI ACCGGT 1 cut(s) 388
AgsI TTSAA 1 cut(s) 482
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 3 cut(s) 20, 295, 418
AluI AGCT 3 cut(s) 20, 295, 418
Alw26I GTCTC 2 cut(s) 347, 417
AlwI GGATC 2 cut(s) 158, 308
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 4, 178
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 242, 274
AsiGI ACCGGT 1 cut(s) 388
Asp718I GGTACC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 6, 306, 577
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 16, 165
BceAI ACGGC 2 cut(s) 290, 394
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 347, 417
BfaI CTAG 1 cut(s) 622
BfmI CTRYAG 1 cut(s) 608
BfoI RGCGCY 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 219
BisI GCNGC 8 cut(s) 5, 89, 92, 95, 98, 101, 179, 276
BlsI GCNGC 8 cut(s) 6, 90, 93, 96, 99, 102, 180, 277
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 2 cut(s) 114, 246
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 114, 246
BmsI GCATC 2 cut(s) 550, 558
BpmI CTGGAG 1 cut(s) 602
Bpu10I CCTNAGC 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 4 cut(s) 112, 145, 168, 245
BsaWI WCCGGW 1 cut(s) 388
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 174, 282
Bse118I RCCGGY 1 cut(s) 388
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 4 cut(s) 112, 145, 168, 245
BseGI GGATG 1 cut(s) 565
BseLI CCNNNNNNNGG 3 cut(s) 113, 174, 282
BseMII CTCAG 2 cut(s) 428, 461
BseRI GAGGAG 1 cut(s) 508
BseXI GCAGC 2 cut(s) 16, 165
Bsh1236I CGCG 2 cut(s) 147, 278
BshFI GGCC 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 388
BsiSI CCGG 2 cut(s) 114, 389
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 3 cut(s) 113, 174, 282
BsmAI GTCTC 2 cut(s) 347, 417
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 117
Bsp143I GATC 4 cut(s) 150, 267, 300, 456
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 427, 460
BspFNI CGCG 2 cut(s) 147, 278
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 1 cut(s) 219
BspPI GGATC 2 cut(s) 158, 308
BspQI GCTCTTC 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 388
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 4 cut(s) 112, 145, 168, 245
BssMI GATC 4 cut(s) 150, 267, 300, 456
BssNI GRCGYC 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 169, 499
Bst6I CTCTTC 2 cut(s) 88, 363
BstACI GRCGYC 1 cut(s) 273
BstAPI GCANNNNNTGC 1 cut(s) 577
BstC8I GCNNGC 2 cut(s) 203, 293
BstDEI CTNAG 4 cut(s) 414, 419, 447, 545
BstDSI CCRYGG 2 cut(s) 145, 168
BstF5I GGATG 1 cut(s) 565
BstFNI CGCG 2 cut(s) 147, 278
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 4 cut(s) 153, 270, 303, 459
BstMAI GTCTC 2 cut(s) 347, 417
BstMBI GATC 4 cut(s) 150, 267, 300, 456
BstMWI GCNNNNNNNGC 8 cut(s) 94, 97, 100, 123, 211, 415, 560, 577
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 2 cut(s) 112, 244
BstSFI CTRYAG 1 cut(s) 608
BstUI CGCG 2 cut(s) 147, 278
BstV1I GCAGC 2 cut(s) 16, 165
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 2 cut(s) 145, 168
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 565
BveI ACCTGC 1 cut(s) 219
Cac8I GCNNGC 2 cut(s) 203, 293
CfoI GCGC 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 1 cut(s) 116
Cfr42I CCGCGG 1 cut(s) 148
CseI GACGC 2 cut(s) 203, 281
Csp6I GTAC 3 cut(s) 71, 322, 451
CspAI ACCGGT 1 cut(s) 388
CviAII CATG 2 cut(s) 52, 238
CviQI GTAC 3 cut(s) 71, 322, 451
DdeI CTNAG 4 cut(s) 414, 419, 447, 545
DpnI GATC 4 cut(s) 152, 269, 302, 458
DpnII GATC 4 cut(s) 150, 267, 300, 456
Eam1104I CTCTTC 2 cut(s) 88, 363
EarI CTCTTC 2 cut(s) 88, 363
EcoRII CCWGG 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 2 cut(s) 55, 241
FaiI YATR 5 cut(s) 53, 137, 239, 386, 604
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 51, 237
FauI CCCGC 2 cut(s) 140, 166
Fnu4HI GCNGC 8 cut(s) 5, 89, 92, 95, 98, 101, 179, 276
FokI GGATG 1 cut(s) 572
Fsp4HI GCNGC 8 cut(s) 5, 89, 92, 95, 98, 101, 179, 276
FspBI CTAG 1 cut(s) 622
GlaI GCGC 1 cut(s) 477
GluI GCNGC 8 cut(s) 5, 89, 92, 95, 98, 101, 179, 276
GsuI CTGGAG 1 cut(s) 602
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 114, 389
HgaI GACGC 2 cut(s) 203, 281
HhaI GCGC 1 cut(s) 478
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 2 cut(s) 55, 241
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 4 cut(s) 280, 329, 368, 515
HpaII CCGG 2 cut(s) 114, 389
HphI GGTGA 3 cut(s) 6, 306, 577
Hpy188I TCNGA 4 cut(s) 33, 285, 538, 559
Hpy188III TCNNGA 1 cut(s) 460
Hpy99I CGWCG 2 cut(s) 131, 275
HpyAV CCTTC 2 cut(s) 520, 584
HpyCH4III ACNGT 2 cut(s) 169, 499
HpyCH4IV ACGT 3 cut(s) 60, 129, 402
HpyCH4V TGCA 4 cut(s) 4, 291, 399, 571
HpyF10VI GCNNNNNNNGC 8 cut(s) 94, 97, 100, 123, 211, 415, 560, 577
HpyF3I CTNAG 4 cut(s) 414, 419, 447, 545
HpySE526I ACGT 3 cut(s) 60, 129, 402
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 2 cut(s) 55, 241
HspAI GCGC 1 cut(s) 476
KpnI GGTACC 1 cut(s) 74
KspI CCGCGG 1 cut(s) 148
Kzo9I GATC 4 cut(s) 150, 267, 300, 456
LguI GCTCTTC 1 cut(s) 363
Lsp1109I GCAGC 2 cut(s) 16, 165
LweI GCATC 2 cut(s) 550, 558
MaeI CTAG 1 cut(s) 622
MaeII ACGT 3 cut(s) 60, 129, 402
MalI GATC 4 cut(s) 152, 269, 302, 458
MboI GATC 4 cut(s) 150, 267, 300, 456
MboII GAAGA 3 cut(s) 75, 350, 494
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 2 cut(s) 481, 516
MnlI CCTC 8 cut(s) 89, 114, 372, 456, 456, 529, 544, 577
MseI TTAA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 2 cut(s) 114, 389
MspR9I CCNGG 2 cut(s) 114, 246
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 2 cut(s) 147, 278
MwoI GCNNNNNNNGC 8 cut(s) 94, 97, 100, 123, 211, 415, 560, 577
NciI CCSGG 1 cut(s) 114
NdeII GATC 4 cut(s) 150, 267, 300, 456
NlaIII CATG 2 cut(s) 55, 241
NlaIV GGNNCC 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 363
PfeI GAWTC 3 cut(s) 280, 329, 368
PinAI ACCGGT 1 cut(s) 388
PkrI GCNGC 8 cut(s) 6, 90, 93, 96, 99, 102, 180, 277
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 116
RsaI GTAC 3 cut(s) 72, 323, 452
RsaNI GTAC 3 cut(s) 71, 322, 451
SacII CCGCGG 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 8 cut(s) 5, 89, 92, 95, 98, 101, 179, 276
Sau3AI GATC 4 cut(s) 150, 267, 300, 456
Sau96I GGNCC 1 cut(s) 116
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 2 cut(s) 114, 246
SfaNI GCATC 2 cut(s) 550, 558
SfcI CTRYAG 1 cut(s) 608
Sfr303I CCGCGG 1 cut(s) 148
SgrBI CCGCGG 1 cut(s) 148
SspMI CTAG 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 112, 244
TaaI ACNGT 2 cut(s) 169, 499
TaiI ACGT 3 cut(s) 63, 132, 405
TaqI TCGA 4 cut(s) 153, 270, 459, 513
TatI WGTACW 1 cut(s) 321
TauI GCSGC 6 cut(s) 91, 94, 97, 100, 103, 278
TfiI GAWTC 3 cut(s) 280, 329, 368
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseI GCWGC 2 cut(s) 4, 178
TspDTI ATGAA 2 cut(s) 124, 254
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XspI CTAG 1 cut(s) 622
ZrmI AGTACT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.