Rh5CG063700

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017097
Physical Location & Seq
Reverse (-)
4846671 .. 4847198
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG063700.1

Sequence Viewer

Length: 528 bp
ATGCAGCGTTCATCACCAGCTTTGGTTCGTCAGATACTCCACCGCAACCGCATGAGCAAAGTCCAAATGGTACCGACCCTCTCTTCGCTGCCGCCGCCGCCTCCCCCTCCCGGCCCACCCTGCGACGTGTTCATAAACCACCGCGGGATCGACACCAAGAGAACCGTGGCGGGGCTGCTGAACTACCACCTGTCCAGCAGGCTCAACCTGCGTCCTTTTTTGGACAGCAAGAACATGAAGCCAGGGGACAGGTTGTTTGATAAGATCGACGCAGCGATTCGGAAATGCAAGCTTGGGATCGCCGTGTTTTCACCGCAATACTGCGAATCTTACTTCTGTCTCCACGAACTCGCTCTTCTAATGGAATCTAAAAAGAGGGTTATACCGGTTTTCTGCAACGTGAAGCCGTCTCAGCTTAGGGTTAAGGATAATGGAGTTTGCCCTCAGTACGAGATCGAGAGGTTTAGTTTGGCGCTTGAAGAAGCCAAATACACAGTTGGACTTACCTTCGACTCCTCTAAAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR TIR_2

Protein Analysis

175

Amino Acids

19.78

Weight (kDa)

9.6

Isoelectric Point (pI)

59.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 42 - 140 1.8e-15 TIR domain
TIR_2 PF13676 43 - 136 1.4e-15 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61105
fragaria_vesca FvH4_3g04640
malus_domestica MD10G1299700.v1.1
prunus_persica Prupe.4G041100_v2.0.a1
rosa_laevigata RLG00000031470
rosa_multiflora Rmu_co8469441.1_g000001 Rmu_ssc0000182.1_g000021
rosa_rugosa Rorug04G0428700
rosa_samantha Rh5AG056600 Rh5BG054000 Rh5CG063700 Rh5DG053000
rosa_wichuraiana Rw5G005380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 70
AccII CGCG 1 cut(s) 144
AciI CCGC 9 cut(s) 43, 49, 92, 95, 98, 142, 144, 170, 314
AclWI GGATC 2 cut(s) 155, 305
AfaI GTAC 2 cut(s) 72, 449
AfiI CCNNNNNNNGG 2 cut(s) 110, 171
AflIII ACRYGT 1 cut(s) 126
AgeI ACCGGT 1 cut(s) 385
AgsI TTSAA 1 cut(s) 479
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 3 cut(s) 20, 292, 415
AluI AGCT 3 cut(s) 20, 292, 415
Alw26I GTCTC 2 cut(s) 344, 414
AlwI GGATC 2 cut(s) 155, 305
AoxI GGCC 1 cut(s) 112
ApeKI GCWGC 4 cut(s) 4, 88, 175, 272
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 239, 271
AsiGI ACCGGT 1 cut(s) 385
Asp718I GGTACC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 6, 303
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 4 cut(s) 16, 75, 162, 284
BceAI ACGGC 2 cut(s) 287, 391
BciT130I CCWGG 1 cut(s) 243
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 344, 414
BfoI RGCGCY 1 cut(s) 476
BfuAI ACCTGC 1 cut(s) 216
BisI GCNGC 7 cut(s) 5, 89, 92, 95, 98, 176, 273
BlsI GCNGC 7 cut(s) 6, 90, 93, 96, 99, 177, 274
Bme1390I CCNGG 2 cut(s) 111, 243
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 111, 243
Bpu10I CCTNAGC 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 142, 165, 242
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 171
Bse118I RCCGGY 1 cut(s) 385
BseBI CCWGG 1 cut(s) 243
BseDI CCNNGG 3 cut(s) 142, 165, 242
BseLI CCNNNNNNNGG 2 cut(s) 110, 171
BseMII CTCAG 2 cut(s) 425, 458
BseRI GAGGAG 1 cut(s) 505
BseXI GCAGC 4 cut(s) 16, 75, 162, 284
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 1 cut(s) 114
BshNI GGYRCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 385
BsiSI CCGG 2 cut(s) 111, 386
BslFI GGGAC 1 cut(s) 260
BslI CCNNNNNNNGG 2 cut(s) 110, 171
BsmAI GTCTC 2 cut(s) 344, 414
BsmBI CGTCTC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 260
BsnI GGCC 1 cut(s) 114
Bsp143I GATC 4 cut(s) 147, 264, 297, 453
BspACI CCGC 9 cut(s) 43, 49, 92, 95, 98, 142, 144, 170, 314
BspANI GGCC 1 cut(s) 114
BspCNI CTCAG 2 cut(s) 424, 457
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 72
BspMI ACCTGC 1 cut(s) 216
BspPI GGATC 2 cut(s) 155, 305
BspQI GCTCTTC 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 385
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 142, 165, 242
BssMI GATC 4 cut(s) 147, 264, 297, 453
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 166, 496
Bst6I CTCTTC 2 cut(s) 88, 360
BstC8I GCNNGC 2 cut(s) 200, 290
BstDEI CTNAG 3 cut(s) 411, 416, 444
BstDSI CCRYGG 2 cut(s) 142, 165
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstKTI GATC 4 cut(s) 150, 267, 300, 456
BstMAI GTCTC 2 cut(s) 344, 414
BstMBI GATC 4 cut(s) 147, 264, 297, 453
BstMWI GCNNNNNNNGC 5 cut(s) 94, 97, 120, 208, 412
BstNI CCWGG 1 cut(s) 243
BstSCI CCNGG 2 cut(s) 109, 241
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 4 cut(s) 16, 75, 162, 284
BsuRI GGCC 1 cut(s) 114
BtgI CCRYGG 2 cut(s) 142, 165
BtrI CACGTC 1 cut(s) 127
BveI ACCTGC 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 200, 290
CfoI GCGC 1 cut(s) 475
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 1 cut(s) 113
Cfr42I CCGCGG 1 cut(s) 145
CseI GACGC 2 cut(s) 200, 278
Csp6I GTAC 2 cut(s) 71, 448
CspAI ACCGGT 1 cut(s) 385
CviAII CATG 2 cut(s) 52, 235
CviJI RGCY 9 cut(s) 20, 114, 175, 202, 241, 292, 406, 415, 485
CviKI_1 RGCY 9 cut(s) 20, 114, 175, 202, 241, 292, 406, 415, 485
CviQI GTAC 2 cut(s) 71, 448
DdeI CTNAG 3 cut(s) 411, 416, 444
DpnI GATC 4 cut(s) 149, 266, 299, 455
DpnII GATC 4 cut(s) 147, 264, 297, 453
Eam1104I CTCTTC 2 cut(s) 88, 360
EarI CTCTTC 2 cut(s) 88, 360
EcoRII CCWGG 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 414
FaeI CATG 2 cut(s) 55, 238
FaiI YATR 4 cut(s) 53, 134, 236, 383
FaqI GGGAC 1 cut(s) 260
FatI CATG 2 cut(s) 51, 234
FauI CCCGC 2 cut(s) 137, 163
Fnu4HI GCNGC 7 cut(s) 5, 89, 92, 95, 98, 176, 273
Fsp4HI GCNGC 7 cut(s) 5, 89, 92, 95, 98, 176, 273
GlaI GCGC 1 cut(s) 474
GluI GCNGC 7 cut(s) 5, 89, 92, 95, 98, 176, 273
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 1 cut(s) 114
HapII CCGG 2 cut(s) 111, 386
HgaI GACGC 2 cut(s) 200, 278
HhaI GCGC 1 cut(s) 475
Hin1II CATG 2 cut(s) 55, 238
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 4 cut(s) 277, 326, 365, 512
HpaII CCGG 2 cut(s) 111, 386
HphI GGTGA 2 cut(s) 6, 303
Hpy188I TCNGA 2 cut(s) 33, 282
Hpy188III TCNNGA 1 cut(s) 457
Hpy99I CGWCG 2 cut(s) 128, 272
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 166, 496
HpyCH4IV ACGT 2 cut(s) 126, 399
HpyCH4V TGCA 3 cut(s) 4, 288, 396
HpyF10VI GCNNNNNNNGC 5 cut(s) 94, 97, 120, 208, 412
HpyF3I CTNAG 3 cut(s) 411, 416, 444
HpySE526I ACGT 2 cut(s) 126, 399
Hsp92II CATG 2 cut(s) 55, 238
HspAI GCGC 1 cut(s) 473
KpnI GGTACC 1 cut(s) 74
KspI CCGCGG 1 cut(s) 145
Kzo9I GATC 4 cut(s) 147, 264, 297, 453
LguI GCTCTTC 1 cut(s) 360
Lsp1109I GCAGC 4 cut(s) 16, 75, 162, 284
MaeII ACGT 2 cut(s) 126, 399
MalI GATC 4 cut(s) 149, 266, 299, 455
MboI GATC 4 cut(s) 147, 264, 297, 453
MboII GAAGA 3 cut(s) 75, 347, 491
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 7 cut(s) 89, 111, 117, 369, 453, 453, 526
MseI TTAA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 2 cut(s) 111, 386
MspR9I CCNGG 2 cut(s) 111, 243
MvaI CCWGG 1 cut(s) 243
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 5 cut(s) 94, 97, 120, 208, 412
NciI CCSGG 1 cut(s) 111
NdeII GATC 4 cut(s) 147, 264, 297, 453
NlaIII CATG 2 cut(s) 55, 238
NlaIV GGNNCC 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 360
PfeI GAWTC 3 cut(s) 277, 326, 365
PinAI ACCGGT 1 cut(s) 385
PkrI GCNGC 7 cut(s) 6, 90, 93, 96, 99, 177, 274
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 113
RsaI GTAC 2 cut(s) 72, 449
RsaNI GTAC 2 cut(s) 71, 448
SacII CCGCGG 1 cut(s) 145
SapI GCTCTTC 1 cut(s) 360
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 7 cut(s) 5, 89, 92, 95, 98, 176, 273
Sau3AI GATC 4 cut(s) 147, 264, 297, 453
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 2 cut(s) 111, 243
Sfr303I CCGCGG 1 cut(s) 145
SgrBI CCGCGG 1 cut(s) 145
SsiI CCGC 9 cut(s) 43, 49, 92, 95, 98, 142, 144, 170, 314
StyD4I CCNGG 2 cut(s) 109, 241
TaaI ACNGT 2 cut(s) 166, 496
TaiI ACGT 2 cut(s) 129, 402
TaqI TCGA 4 cut(s) 150, 267, 456, 510
TauI GCSGC 3 cut(s) 94, 97, 100
TfiI GAWTC 3 cut(s) 277, 326, 365
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TseI GCWGC 4 cut(s) 4, 88, 175, 272
TspDTI ATGAA 2 cut(s) 121, 251
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.