RLG00000031520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018425
Physical Location & Seq
Forward (+)
5888971 .. 5890760
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031520

Sequence Viewer

Length: 729 bp
ATGGCATCTTGCCCAGAATTCAACAATTCCCAAAGAACAAATGAAATTTCCCAGAAATTTAAGCACATTCTTGAAGATGCTGACTCTAAAATTGCTGAGCTCCTTGAACAAAATGGTGAGGCTTCCCCTGAAATTCTTCAAAACATTCTTCAAGCCCAGAAATCGATCAAAGATTTGATCTTGGAGTACAACCCTAGCTCAACTGAAGATGGGTTCAGTTTTGAGGTACCACCTCATTTTCAAGCCACTCCCGAGAGCTCTGCACAACCATATTTGCATTACTGGAAGCTGCTTCAGTCCGTCTCTGGAATCTTGAAGGCGATAACAAAGTTGATGGGCATTCTGGCCAGCTTTCCCGGCAGCGGCATCGTCGGCACCGCCGTCATCGGCTTCCAACTGGCCCAACTCGCTGAGGAAGCCAAGGGGCTTCTTGGTGATCTCATCCAGACTCTTACAGCCATGGCCACAAGGCGAAAACAGAAGATTCAAGTTGGGACAGTGATGGATCATATCCTTTCTACAGTGACTATGGACCTCGGCCAGTATACCCTGATTGATCGAGCGCCGTCCACCTCCGGCAGCGGCCGCGGCGGTGGCGACGCGGAGGAGGAAGCGCCGGAAGGCTCAGCAGGACGCCTTTCCTTAACTATGAGTGCAATTGTCAATGTTGTGTTTCCCAATCCGTGCAAATTGAAGAGAGGATATCAGGCACAACTCTTTGACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

26.23

Weight (kDa)

5.29

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018425)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26662
rosa_chinensis RchiOBHm_Chr4g0419801 RchiOBHm_Chr4g0419851 RchiOBHm_Chr4g0419861
rosa_laevigata RLG00000031520
rosa_roxburghii Rroxscaffold_5G00362610
rosa_samantha Rh4CG235200 Rh4CG235300 Rh5AG060100 Rh5CG066600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 2 cut(s) 226, 374
AccI GTMKAC 1 cut(s) 545
AccII CGCG 2 cut(s) 588, 602
AciI CCGC 7 cut(s) 363, 378, 582, 586, 588, 591, 602
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 4 cut(s) 345, 462, 538, 583
AcsI RAATTY 4 cut(s) 17, 45, 56, 132
AcuI CTGAAG 2 cut(s) 225, 278
AcyI GRCGYC 1 cut(s) 634
AfaI GTAC 2 cut(s) 188, 228
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 9 cut(s) 22, 74, 107, 140, 152, 242, 316, 488, 694
AluBI AGCT 5 cut(s) 100, 198, 258, 289, 351
AluI AGCT 5 cut(s) 100, 198, 258, 289, 351
Alw21I GWGCWC 2 cut(s) 102, 260
Alw26I GTCTC 1 cut(s) 307
AlwI GGATC 1 cut(s) 513
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 5 cut(s) 345, 399, 462, 538, 583
ApeKI GCWGC 3 cut(s) 289, 360, 579
ApoI RAATTY 4 cut(s) 17, 45, 56, 132
Asp700I GAANNNNTTC 1 cut(s) 135
Asp718I GGTACC 1 cut(s) 226
AspLEI GCGC 2 cut(s) 565, 616
AspS9I GGNCC 2 cut(s) 400, 532
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 2 cut(s) 128, 446
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 532
BalI TGGCCA 2 cut(s) 347, 464
BanI GGYRCC 2 cut(s) 226, 374
BanII GRGCYC 2 cut(s) 102, 260
Bbv12I GWGCWC 2 cut(s) 102, 260
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 3 cut(s) 276, 372, 591
BccI CCATC 3 cut(s) 203, 328, 496
BceAI ACGGC 2 cut(s) 365, 550
BcgI CGANNNNNNTGC 4 cut(s) 242, 276, 349, 383
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 307
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 2 cut(s) 566, 617
BisI GCNGC 7 cut(s) 290, 361, 364, 580, 583, 586, 589
BlpI GCTNAGC 2 cut(s) 96, 625
BlsI GCNGC 7 cut(s) 291, 362, 365, 581, 584, 587, 590
Bme1390I CCNGG 1 cut(s) 357
Bme18I GGWCC 1 cut(s) 532
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 400, 532
BmiI GGNNCC 2 cut(s) 228, 376
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 3 cut(s) 14, 67, 375
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 2 cut(s) 96, 625
BpuMI CCSGG 1 cut(s) 357
Bsa29I ATCGAT 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 634
BsaJI CCNNGG 4 cut(s) 420, 459, 535, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse1I ACTGG 3 cut(s) 287, 402, 541
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 4 cut(s) 420, 459, 535, 586
BseGI GGATG 1 cut(s) 441
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMII CTCAG 3 cut(s) 87, 402, 639
BseNI ACTGG 3 cut(s) 287, 402, 541
BseRI GAGGAG 1 cut(s) 620
BseX3I CGGCCG 1 cut(s) 583
BseXI GCAGC 3 cut(s) 276, 372, 591
BsgI GTGCAG 1 cut(s) 246
Bsh1236I CGCG 2 cut(s) 588, 602
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 5 cut(s) 347, 401, 464, 540, 585
BshNI GGYRCC 2 cut(s) 226, 374
BshVI ATCGAT 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 586
BsiHKAI GWGCWC 2 cut(s) 102, 260
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 3 cut(s) 357, 576, 617
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 307
BsmBI CGTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 5 cut(s) 347, 401, 464, 540, 585
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 2 cut(s) 102, 260
Bsp143I GATC 5 cut(s) 165, 177, 436, 505, 556
Bsp1720I GCTNAGC 2 cut(s) 96, 625
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 7 cut(s) 363, 378, 582, 586, 588, 591, 602
BspANI GGCC 5 cut(s) 347, 401, 464, 540, 585
BspCNI CTCAG 3 cut(s) 88, 403, 638
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 2 cut(s) 588, 602
BspLI GGNNCC 2 cut(s) 228, 376
BspPI GGATC 1 cut(s) 513
BspT107I GGYRCC 2 cut(s) 226, 374
BsrI ACTGG 3 cut(s) 287, 402, 541
BssECI CCNNGG 4 cut(s) 420, 459, 535, 586
BssMI GATC 5 cut(s) 165, 177, 436, 505, 556
BssNAI GTATAC 1 cut(s) 546
BssNI GRCGYC 1 cut(s) 634
BssT1I CCWWGG 2 cut(s) 420, 459
Bst1107I GTATAC 1 cut(s) 546
Bst4CI ACNGT 2 cut(s) 499, 523
Bst6I CTCTTC 1 cut(s) 689
BstACI GRCGYC 1 cut(s) 634
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 3 cut(s) 96, 411, 625
BstDSI CCRYGG 2 cut(s) 459, 586
BstF5I GGATG 1 cut(s) 441
BstFNI CGCG 2 cut(s) 588, 602
BstH2I RGCGCY 2 cut(s) 566, 617
BstHHI GCGC 2 cut(s) 565, 616
BstKTI GATC 5 cut(s) 168, 180, 439, 508, 559
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 5 cut(s) 165, 177, 436, 505, 556
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 7 cut(s) 357, 372, 407, 416, 585, 588, 594
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 2 cut(s) 588, 602
BstV1I GCAGC 3 cut(s) 276, 372, 591
BstZ17I GTATAC 1 cut(s) 546
BstZI CGGCCG 1 cut(s) 583
Bsu15I ATCGAT 1 cut(s) 164
BsuRI GGCC 5 cut(s) 347, 401, 464, 540, 585
BsuTUI ATCGAT 1 cut(s) 164
BtgI CCRYGG 2 cut(s) 459, 586
BtsCI GGATG 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 504, 528
Cac8I GCNNGC 1 cut(s) 349
CciNI GCGGCCGC 1 cut(s) 583
CfoI GCGC 2 cut(s) 565, 616
Cfr13I GGNCC 2 cut(s) 400, 532
Cfr42I CCGCGG 1 cut(s) 589
ClaI ATCGAT 1 cut(s) 164
CseI GACGC 2 cut(s) 608, 642
Csp6I GTAC 2 cut(s) 187, 227
CviAII CATG 1 cut(s) 460
CviQI GTAC 2 cut(s) 187, 227
DdeI CTNAG 3 cut(s) 96, 411, 625
DpnI GATC 5 cut(s) 167, 179, 438, 507, 558
DpnII GATC 5 cut(s) 165, 177, 436, 505, 556
EaeI YGGCCR 4 cut(s) 345, 462, 538, 583
EagI CGGCCG 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Ecl136II GAGCTC 2 cut(s) 100, 258
EclXI CGGCCG 1 cut(s) 583
Eco130I CCWWGG 2 cut(s) 420, 459
Eco24I GRGCYC 2 cut(s) 102, 260
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 532
Eco52I CGGCCG 1 cut(s) 583
Eco53kI GAGCTC 2 cut(s) 100, 258
Eco57I CTGAAG 2 cut(s) 225, 278
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 2 cut(s) 100, 258
EcoRI GAATTC 1 cut(s) 17
EcoRV GATATC 1 cut(s) 704
EcoT14I CCWWGG 2 cut(s) 420, 459
EcoT38I GRGCYC 2 cut(s) 102, 260
ErhI CCWWGG 2 cut(s) 420, 459
Esp3I CGTCTC 1 cut(s) 307
FaeI CATG 1 cut(s) 463
FaiI YATR 6 cut(s) 271, 461, 510, 530, 546, 650
FaqI GGGAC 1 cut(s) 508
FatI CATG 1 cut(s) 459
FblI GTMKAC 1 cut(s) 545
Fnu4HI GCNGC 7 cut(s) 290, 361, 364, 580, 583, 586, 589
FokI GGATG 1 cut(s) 428
FriOI GRGCYC 2 cut(s) 102, 260
Fsp4HI GCNGC 7 cut(s) 290, 361, 364, 580, 583, 586, 589
FspBI CTAG 1 cut(s) 195
GlaI GCGC 2 cut(s) 564, 615
GluI GCNGC 7 cut(s) 290, 361, 364, 580, 583, 586, 589
HaeII RGCGCY 2 cut(s) 566, 617
HaeIII GGCC 5 cut(s) 347, 401, 464, 540, 585
HapII CCGG 3 cut(s) 357, 576, 617
HgaI GACGC 2 cut(s) 608, 642
HhaI GCGC 2 cut(s) 565, 616
Hin1I GRCGYC 1 cut(s) 634
Hin1II CATG 1 cut(s) 463
Hin6I GCGC 2 cut(s) 563, 614
HinP1I GCGC 2 cut(s) 563, 614
HinfI GANTC 4 cut(s) 83, 309, 448, 484
HpaII CCGG 3 cut(s) 357, 576, 617
HphI GGTGA 2 cut(s) 128, 446
Hpy166II GTNNAC 2 cut(s) 546, 570
Hpy188III TCNNGA 5 cut(s) 71, 251, 306, 313, 445
Hpy8I GTNNAC 2 cut(s) 546, 570
Hpy99I CGWCG 2 cut(s) 374, 602
HpyAV CCTTC 2 cut(s) 310, 614
HpyCH4III ACNGT 2 cut(s) 499, 523
HpyCH4V TGCA 4 cut(s) 263, 277, 656, 687
HpyF10VI GCNNNNNNNGC 7 cut(s) 357, 372, 407, 416, 585, 588, 594
HpyF3I CTNAG 3 cut(s) 96, 411, 625
Hsp92I GRCGYC 1 cut(s) 634
Hsp92II CATG 1 cut(s) 463
HspAI GCGC 2 cut(s) 563, 614
KpnI GGTACC 1 cut(s) 230
KspI CCGCGG 1 cut(s) 589
Kzo9I GATC 5 cut(s) 165, 177, 436, 505, 556
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 3 cut(s) 276, 372, 591
LweI GCATC 3 cut(s) 14, 67, 375
MaeI CTAG 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 523
MalI GATC 5 cut(s) 167, 179, 438, 507, 558
MboI GATC 5 cut(s) 165, 177, 436, 505, 556
MboII GAAGA 6 cut(s) 86, 128, 140, 218, 493, 706
MfeI CAATTG 1 cut(s) 657
MhlI GDGCHC 2 cut(s) 102, 260
MlsI TGGCCA 2 cut(s) 347, 464
MluCI AATT 8 cut(s) 17, 25, 45, 56, 90, 132, 657, 689
MluNI TGGCCA 2 cut(s) 347, 464
MlyI GAGTC 2 cut(s) 77, 442
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 9 cut(s) 112, 217, 243, 406, 545, 583, 598, 601, 692
Mox20I TGGCCA 2 cut(s) 347, 464
MroXI GAANNNNTTC 1 cut(s) 135
MscI TGGCCA 2 cut(s) 347, 464
MseI TTAA 2 cut(s) 60, 644
Msp20I TGGCCA 2 cut(s) 347, 464
MspA1I CMGCKG 3 cut(s) 363, 582, 588
MspI CCGG 3 cut(s) 357, 576, 617
MspR9I CCNGG 1 cut(s) 357
MunI CAATTG 1 cut(s) 657
Mva1269I GAATGC 1 cut(s) 339
MvnI CGCG 2 cut(s) 588, 602
MwoI GCNNNNNNNGC 7 cut(s) 357, 372, 407, 416, 585, 588, 594
NciI CCSGG 1 cut(s) 357
NcoI CCATGG 1 cut(s) 459
NdeII GATC 5 cut(s) 165, 177, 436, 505, 556
NlaIII CATG 1 cut(s) 463
NlaIV GGNNCC 2 cut(s) 228, 376
NmeAIII GCCGAG 1 cut(s) 516
NmuCI GTSAC 1 cut(s) 523
NotI GCGGCCGC 1 cut(s) 583
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PctI GAATGC 1 cut(s) 339
PdmI GAANNNNTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 309, 484
PkrI GCNGC 7 cut(s) 291, 362, 365, 581, 584, 587, 590
PleI GAGTC 2 cut(s) 77, 442
PpsI GAGTC 2 cut(s) 77, 442
Psp124BI GAGCTC 2 cut(s) 102, 260
PspN4I GGNNCC 2 cut(s) 228, 376
PspPI GGNCC 2 cut(s) 400, 532
RsaI GTAC 2 cut(s) 188, 228
RsaNI GTAC 2 cut(s) 187, 227
SacI GAGCTC 2 cut(s) 102, 260
SacII CCGCGG 1 cut(s) 589
SaqAI TTAA 2 cut(s) 60, 644
SatI GCNGC 7 cut(s) 290, 361, 364, 580, 583, 586, 589
Sau3AI GATC 5 cut(s) 165, 177, 436, 505, 556
Sau96I GGNCC 2 cut(s) 400, 532
SchI GAGTC 2 cut(s) 77, 442
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 2 cut(s) 102, 260
SetI ASST 9 cut(s) 102, 200, 228, 235, 260, 291, 353, 537, 575
SfaNI GCATC 3 cut(s) 14, 67, 375
SfcI CTRYAG 1 cut(s) 519
Sfr303I CCGCGG 1 cut(s) 589
SgrBI CCGCGG 1 cut(s) 589
SinI GGWCC 1 cut(s) 532
Sse9I AATT 8 cut(s) 17, 25, 45, 56, 90, 132, 657, 689
SsiI CCGC 7 cut(s) 363, 378, 582, 586, 588, 591, 602
SspMI CTAG 1 cut(s) 195
SstI GAGCTC 2 cut(s) 102, 260
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 2 cut(s) 420, 459
TaaI ACNGT 2 cut(s) 499, 523
TaqI TCGA 2 cut(s) 164, 559
TasI AATT 8 cut(s) 17, 25, 45, 56, 90, 132, 657, 689
TatI WGTACW 1 cut(s) 186
TauI GCSGC 4 cut(s) 366, 585, 588, 591
TfiI GAWTC 2 cut(s) 309, 484
Tru1I TTAA 2 cut(s) 60, 644
Tru9I TTAA 2 cut(s) 60, 644
TscAI CASTG 2 cut(s) 504, 528
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 3 cut(s) 289, 360, 579
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 1 cut(s) 57
TspGWI ACGGA 2 cut(s) 289, 672
TspRI CASTG 2 cut(s) 504, 528
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 4 cut(s) 17, 45, 56, 132
XmiI GTMKAC 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.