Rh5AG060100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018425
Physical Location & Seq
Forward (+)
5130542 .. 5131680
1139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG060100.1

Sequence Viewer

Length: 555 bp
ATGGCATCTTGCCCAGAATTCAACAATTCCCAAAGAACAAATGAAATTTCCCAGAAATTTAAGCACATTCTTGAAGATGCTGACTCTAAAATTGCTGAGCTCATTGAACAAAATGGTGAGGCTTCCCCTGAAATTCTTCAAAACATTCTTCAAGCCCAGAAATCGATCAAAGATTTGATCTTGGAGTACAACCCTAGCTCAACTGAAGATGGGTTCAGTTTTGAGGTACCACCTCATTTTCAAGCCACTCCCGAGAGCTCTGCACAACCATATTGGCATTACTGGAAGCTGCTTCAGTCCGTCACTGGAATCTTGAAGGCGATAAAAAAGTTGATGGGCATTCTGGCCAGCTTTCCCGCCGGCGGCATCGTCGGCACCGCCGTCATCGTCGGCACCGCCGTCATCGGCTTCCAACTGGCCCAACTCGCTGCGGAAGCCATGGGGCTTCTTCGTGATCTCATCCAGAATCTTACAGCCCTGGCCACAAAGAGAAAGCAGGAGATTCAAGTTGAGACATTACCAGAAAAACGAGTAAAGATGGAGAGTAACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.28

Weight (kDa)

5.44

Isoelectric Point (pI)

62.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018425)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g26662
rosa_chinensis RchiOBHm_Chr4g0419801 RchiOBHm_Chr4g0419851 RchiOBHm_Chr4g0419861
rosa_laevigata RLG00000031520
rosa_roxburghii Rroxscaffold_5G00362610
rosa_samantha Rh4CG235200 Rh4CG235300 Rh5AG060100 Rh5CG066600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 226
AccB1I GGYRCC 3 cut(s) 226, 374, 392
AciI CCGC 5 cut(s) 357, 363, 378, 396, 431
AcoI YGGCCR 2 cut(s) 345, 480
AcsI RAATTY 4 cut(s) 17, 45, 56, 132
AcuI CTGAAG 2 cut(s) 225, 278
AfaI GTAC 2 cut(s) 188, 228
AfiI CCNNNNNNNGG 1 cut(s) 362
AgsI TTSAA 8 cut(s) 22, 74, 107, 140, 152, 242, 316, 506
AjnI CCWGG 1 cut(s) 477
AluBI AGCT 6 cut(s) 100, 198, 258, 289, 351, 552
AluI AGCT 6 cut(s) 100, 198, 258, 289, 351, 552
Alw21I GWGCWC 2 cut(s) 102, 260
Alw26I GTCTC 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 3 cut(s) 345, 417, 480
ApeKI GCWGC 2 cut(s) 289, 428
ApoI RAATTY 4 cut(s) 17, 45, 56, 132
Asp700I GAANNNNTTC 1 cut(s) 135
Asp718I GGTACC 1 cut(s) 226
AspS9I GGNCC 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 251
BalI TGGCCA 2 cut(s) 347, 482
BanI GGYRCC 3 cut(s) 226, 374, 392
BanII GRGCYC 2 cut(s) 102, 260
Bbv12I GWGCWC 2 cut(s) 102, 260
BbvI GCAGC 2 cut(s) 276, 415
BccI CCATC 3 cut(s) 203, 328, 532
BceAI ACGGC 2 cut(s) 365, 383
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 506
BfaI CTAG 2 cut(s) 195, 553
BisI GCNGC 3 cut(s) 290, 364, 429
BlpI GCTNAGC 1 cut(s) 96
BlsI GCNGC 3 cut(s) 291, 365, 430
Bme1390I CCNGG 1 cut(s) 479
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 3 cut(s) 228, 376, 394
BmrFI CCNGG 1 cut(s) 479
BmsI GCATC 3 cut(s) 14, 67, 375
Bpu1102I GCTNAGC 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 438, 477
Bsc4I CCNNNNNNNGG 1 cut(s) 362
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 287, 310, 420
BseBI CCWGG 1 cut(s) 479
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 438, 477
BseGI GGATG 1 cut(s) 459
BseLI CCNNNNNNNGG 1 cut(s) 362
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 3 cut(s) 287, 310, 420
BseXI GCAGC 2 cut(s) 276, 415
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 3 cut(s) 347, 419, 482
BshNI GGYRCC 3 cut(s) 226, 374, 392
BshVI ATCGAT 1 cut(s) 164
BsiHKAI GWGCWC 2 cut(s) 102, 260
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 360
BslI CCNNNNNNNGG 1 cut(s) 362
BsmAI GTCTC 1 cut(s) 506
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 3 cut(s) 347, 419, 482
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 2 cut(s) 102, 260
Bsp143I GATC 3 cut(s) 165, 177, 454
Bsp1720I GCTNAGC 1 cut(s) 96
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 5 cut(s) 357, 363, 378, 396, 431
BspANI GGCC 3 cut(s) 347, 419, 482
BspCNI CTCAG 1 cut(s) 88
BspDI ATCGAT 1 cut(s) 164
BspLI GGNNCC 3 cut(s) 228, 376, 394
BspT107I GGYRCC 3 cut(s) 226, 374, 392
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 287, 310, 420
BssAI RCCGGY 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 438, 477
BssMI GATC 3 cut(s) 165, 177, 454
BssT1I CCWWGG 1 cut(s) 438
Bst2UI CCWGG 1 cut(s) 479
BstC8I GCNNGC 2 cut(s) 349, 361
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 459
BstKTI GATC 3 cut(s) 168, 180, 457
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 3 cut(s) 165, 177, 454
BstMWI GCNNNNNNNGC 3 cut(s) 372, 425, 434
BstNI CCWGG 1 cut(s) 479
BstSCI CCNGG 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 276, 415
Bsu15I ATCGAT 1 cut(s) 164
BsuRI GGCC 3 cut(s) 347, 419, 482
BsuTUI ATCGAT 1 cut(s) 164
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 459
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 349, 361
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 418
ClaI ATCGAT 1 cut(s) 164
Csp6I GTAC 2 cut(s) 187, 227
CviAII CATG 1 cut(s) 439
CviQI GTAC 2 cut(s) 187, 227
DdeI CTNAG 1 cut(s) 96
DpnI GATC 3 cut(s) 167, 179, 456
DpnII GATC 3 cut(s) 165, 177, 454
EaeI YGGCCR 2 cut(s) 345, 480
Ecl136II GAGCTC 2 cut(s) 100, 258
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 2 cut(s) 102, 260
Eco53kI GAGCTC 2 cut(s) 100, 258
Eco57I CTGAAG 2 cut(s) 225, 278
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 2 cut(s) 100, 258
EcoRI GAATTC 1 cut(s) 17
EcoRII CCWGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 2 cut(s) 102, 260
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 1 cut(s) 442
FaiI YATR 2 cut(s) 271, 440
FatI CATG 1 cut(s) 438
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 290, 364, 429
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 2 cut(s) 102, 260
Fsp4HI GCNGC 3 cut(s) 290, 364, 429
FspBI CTAG 2 cut(s) 195, 553
GluI GCNGC 3 cut(s) 290, 364, 429
HaeIII GGCC 3 cut(s) 347, 419, 482
HapII CCGG 1 cut(s) 360
Hin1II CATG 1 cut(s) 442
HinfI GANTC 4 cut(s) 83, 309, 466, 502
HpaII CCGG 1 cut(s) 360
HphI GGTGA 1 cut(s) 128
Hpy188III TCNNGA 5 cut(s) 71, 251, 313, 452, 463
Hpy99I CGWCG 2 cut(s) 374, 392
HpyAV CCTTC 1 cut(s) 310
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 3 cut(s) 372, 425, 434
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 1 cut(s) 442
KpnI GGTACC 1 cut(s) 230
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 165, 177, 454
Lsp1109I GCAGC 2 cut(s) 276, 415
LweI GCATC 3 cut(s) 14, 67, 375
MaeI CTAG 2 cut(s) 195, 553
MaeIII GTNAC 2 cut(s) 301, 545
MalI GATC 3 cut(s) 167, 179, 456
MboI GATC 3 cut(s) 165, 177, 454
MboII GAAGA 5 cut(s) 86, 128, 140, 218, 440
MhlI GDGCHC 2 cut(s) 102, 260
MlsI TGGCCA 2 cut(s) 347, 482
MluCI AATT 6 cut(s) 17, 25, 45, 56, 90, 132
MluNI TGGCCA 2 cut(s) 347, 482
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 3 cut(s) 112, 217, 243
Mox20I TGGCCA 2 cut(s) 347, 482
MreI CGCCGGCG 1 cut(s) 359
MroNI GCCGGC 1 cut(s) 359
MroXI GAANNNNTTC 1 cut(s) 135
MscI TGGCCA 2 cut(s) 347, 482
MseI TTAA 1 cut(s) 60
Msp20I TGGCCA 2 cut(s) 347, 482
MspI CCGG 1 cut(s) 360
MspR9I CCNGG 1 cut(s) 479
Mva1269I GAATGC 1 cut(s) 339
MvaI CCWGG 1 cut(s) 479
MwoI GCNNNNNNNGC 3 cut(s) 372, 425, 434
NaeI GCCGGC 1 cut(s) 361
NcoI CCATGG 1 cut(s) 438
NdeII GATC 3 cut(s) 165, 177, 454
NgoMIV GCCGGC 1 cut(s) 359
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 3 cut(s) 228, 376, 394
NmuCI GTSAC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 396
PctI GAATGC 1 cut(s) 339
PdiI GCCGGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 135
PfeI GAWTC 3 cut(s) 309, 466, 502
PkrI GCNGC 3 cut(s) 291, 365, 430
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 2 cut(s) 102, 260
Psp6I CCWGG 1 cut(s) 477
PspGI CCWGG 1 cut(s) 477
PspN4I GGNNCC 3 cut(s) 228, 376, 394
PspPI GGNCC 1 cut(s) 418
RsaI GTAC 2 cut(s) 188, 228
RsaNI GTAC 2 cut(s) 187, 227
SacI GAGCTC 2 cut(s) 102, 260
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 3 cut(s) 290, 364, 429
Sau3AI GATC 3 cut(s) 165, 177, 454
Sau96I GGNCC 1 cut(s) 418
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 479
SduI GDGCHC 2 cut(s) 102, 260
SetI ASST 8 cut(s) 102, 200, 228, 235, 260, 291, 353, 554
SfaNI GCATC 3 cut(s) 14, 67, 375
SgrAI CRCCGGYG 1 cut(s) 359
Sse9I AATT 6 cut(s) 17, 25, 45, 56, 90, 132
SsiI CCGC 5 cut(s) 357, 363, 378, 396, 431
SspMI CTAG 2 cut(s) 195, 553
SstI GAGCTC 2 cut(s) 102, 260
StyD4I CCNGG 1 cut(s) 477
StyI CCWWGG 1 cut(s) 438
TaqI TCGA 1 cut(s) 164
TasI AATT 6 cut(s) 17, 25, 45, 56, 90, 132
TatI WGTACW 1 cut(s) 186
TauI GCSGC 1 cut(s) 366
TfiI GAWTC 3 cut(s) 309, 466, 502
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 2 cut(s) 289, 428
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 1 cut(s) 57
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 1 cut(s) 310
XapI RAATTY 4 cut(s) 17, 45, 56, 132
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 2 cut(s) 195, 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.