RLG00000031848

Component of the peroxisomal and mitochondrial division machineries. Plays a role in promoting the fission of mitochondria and peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017619
Physical Location & Seq
Reverse (-)
8703754 .. 8705066
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031848

Sequence Viewer

Length: 348 bp
ATGGCCAAAAGTACTAACTCCCCTTTGGATCAACGAGAGATGCTTTACCTTCTGGCCGTCAAATACTACAGAAGTGGTGCATATTTAACTAGCCGGCGTCTTCTGGAGCGGTGCTTAGGGATTGAACCAGAATGGAGACAGGCTCTGAGTCTTAAGAAGACAGTTGAGGATCGAATTGTGAAAGATGGCTTCATTGGAATAGGCGTTACTGCTGGCGCTCGAAGTCCAGGCCACCAGCCAAGGCCAGACACCACAAGTCTTTCTCAGTCTACTTCAGACATGCCTCTGAAAGCTAAGGCCAGACAACAGTCGTTCTCAGTCTACTTCAGACATGCCTTTGAAAGTTGA

Protein Analysis

116

Amino Acids

13.0

Weight (kDa)

9.86

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fis1_TPR_C PF14853 12 - 63 6.7e-19 Fis1 C-terminal tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07330
rosa_chinensis RchiOBHm_Chr5g0011611
rosa_laevigata RLG00000031846 RLG00000031848
rosa_multiflora Rmu_co8315781.1_g000001 Rmu_sc0009001.1_g000013
rosa_roxburghii Rroxscaffold_1G00064890
rosa_rugosa Rorug04G0458000
rosa_samantha Rh5BG085200 Rh5CG099100 Rh5DG085000
rosa_wichuraiana Rw5G007800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 2 cut(s) 269, 321
AciI CCGC 1 cut(s) 109
AclWI GGATC 2 cut(s) 36, 177
AcoI YGGCCR 2 cut(s) 3, 54
AcuI CTGAAG 2 cut(s) 258, 310
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 1 cut(s) 13
AflII CTTAAG 1 cut(s) 152
AgsI TTSAA 2 cut(s) 125, 341
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 1 cut(s) 293
AluI AGCT 1 cut(s) 293
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 2 cut(s) 36, 177
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 5 cut(s) 3, 54, 229, 242, 297
AspLEI GCGC 1 cut(s) 218
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 92, 164
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 67
BfoI RGCGCY 1 cut(s) 219
BfrI CTTAAG 1 cut(s) 152
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 30
BoxI GACNNNNGTC 1 cut(s) 307
BpiI GAAGAC 2 cut(s) 92, 164
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpmI CTGGAG 1 cut(s) 125
Bpu10I CCTNAGC 2 cut(s) 115, 294
BsaHI GRCGYC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 93
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 239
BseMII CTCAG 3 cut(s) 137, 278, 330
BshFI GGCC 5 cut(s) 5, 56, 231, 244, 299
BsiSI CCGG 1 cut(s) 94
BsmAI GTCTC 1 cut(s) 130
BsnI GGCC 5 cut(s) 5, 56, 231, 244, 299
Bsp143I GATC 2 cut(s) 28, 169
BspACI CCGC 1 cut(s) 109
BspANI GGCC 5 cut(s) 5, 56, 231, 244, 299
BspCNI CTCAG 3 cut(s) 138, 277, 329
BspPI GGATC 2 cut(s) 36, 177
BspTI CTTAAG 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 93
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 239
BssMI GATC 2 cut(s) 28, 169
BssNI GRCGYC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 2 cut(s) 163, 309
BstACI GRCGYC 1 cut(s) 97
BstAFI CTTAAG 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 95, 214
BstDEI CTNAG 5 cut(s) 115, 146, 264, 294, 316
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 2 cut(s) 31, 172
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 28, 169
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 2 cut(s) 283, 335
BstPAI GACNNNNGTC 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 67
BstV2I GAAGAC 2 cut(s) 92, 164
BsuRI GGCC 5 cut(s) 5, 56, 231, 244, 299
Cac8I GCNNGC 2 cut(s) 95, 214
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 1 cut(s) 218
Cfr10I RCCGGY 1 cut(s) 93
CseI GACGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 12
CviAII CATG 2 cut(s) 280, 332
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 5 cut(s) 115, 146, 264, 294, 316
DpnI GATC 2 cut(s) 30, 171
DpnII GATC 2 cut(s) 28, 169
EaeI YGGCCR 2 cut(s) 3, 54
Eco130I CCWWGG 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 258, 310
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 239
FaeI CATG 2 cut(s) 283, 335
FaiI YATR 3 cut(s) 82, 281, 333
FatI CATG 2 cut(s) 279, 331
FblI GTMKAC 2 cut(s) 269, 321
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 5 cut(s) 5, 56, 231, 244, 299
HapII CCGG 1 cut(s) 94
HgaI GACGC 1 cut(s) 86
HhaI GCGC 1 cut(s) 218
Hin1I GRCGYC 1 cut(s) 97
Hin1II CATG 2 cut(s) 283, 335
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HinfI GANTC 1 cut(s) 148
HpaII CCGG 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 270, 322
Hpy188I TCNGA 4 cut(s) 147, 277, 288, 329
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 2 cut(s) 270, 322
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 163, 309
HpyCH4V TGCA 1 cut(s) 80
HpyF3I CTNAG 5 cut(s) 115, 146, 264, 294, 316
Hsp92I GRCGYC 1 cut(s) 97
Hsp92II CATG 2 cut(s) 283, 335
HspAI GCGC 1 cut(s) 216
KroI GCCGGC 1 cut(s) 93
KroNI GCCGGC 1 cut(s) 95
Kzo9I GATC 2 cut(s) 28, 169
LmnI GCTCC 1 cut(s) 106
LweI GCATC 1 cut(s) 30
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 205
MalI GATC 2 cut(s) 30, 171
MbiI CCGCTC 1 cut(s) 109
MboI GATC 2 cut(s) 28, 169
MboII GAAGA 2 cut(s) 92, 169
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 174
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 2 cut(s) 160, 294
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 93
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 86, 153
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 1 cut(s) 94
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
NaeI GCCGGC 1 cut(s) 95
NdeII GATC 2 cut(s) 28, 169
NgoMIV GCCGGC 1 cut(s) 93
NlaIII CATG 2 cut(s) 283, 335
NspI RCATGY 2 cut(s) 283, 335
PdiI GCCGGC 1 cut(s) 95
PleI GAGTC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 156
PshAI GACNNNNGTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PstNI CAGNNNCTG 1 cut(s) 145
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 86, 153
Sau3AI GATC 2 cut(s) 28, 169
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 1 cut(s) 157
ScrFI CCNGG 1 cut(s) 228
SetI ASST 2 cut(s) 51, 295
SfaNI GCATC 1 cut(s) 30
SfcI CTRYAG 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 1 cut(s) 174
SsiI CCGC 1 cut(s) 109
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 2 cut(s) 163, 309
TaqI TCGA 2 cut(s) 172, 220
TasI AATT 1 cut(s) 174
TatI WGTACW 1 cut(s) 11
Tru1I TTAA 2 cut(s) 86, 153
Tru9I TTAA 2 cut(s) 86, 153
TspDTI ATGAA 1 cut(s) 181
Vha464I CTTAAG 1 cut(s) 152
XceI RCATGY 2 cut(s) 283, 335
XmiI GTMKAC 2 cut(s) 269, 321
XspI CTAG 1 cut(s) 90
ZrmI AGTACT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.