Rh5BG085200

Component of the peroxisomal and mitochondrial division machineries. Plays a role in promoting the fission of mitochondria and peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017619
Physical Location & Seq
Reverse (-)
7370224 .. 7370955
732 bp
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UTR
Exon/CDS
Intron
Rh5BG085200.1

Sequence Viewer

Length: 546 bp
ATGAAAGAGATGATGAACAACATTTTTGACTCCGTCAGTTCCTTGTTTGGCTCCGGAGAAGACCATATACCATGGTGCAATCGAGACATCATCCATAGGTGTGAGATAGAAGTTGGAGAGGCAGTGGACAAAGGTGAGTCGACAGAGGAGCGCAAGAGCAAAAGCATAATGAGGTTATCATGGGCACTTGTTCACTCAATGCAACGTGAAGATGTGCAACGTGGAATTGACATGCTACTACAAGCAACTAATAATTCAATGGCCAAAAGTACTAACTCCCCTTTGGATCAACGAGAGATGCTTTACCTTACGGCCGTTGGATACTACAGAAGTGGTGAATATTCAACTAGCCGGCGTCTTCTGAATCGGTGCTTAGGGATTGAACCAAAATGGAGACGGGCTCTGAGTCTGAAGAAGACAGTTGAGGATCGAATTGTGAAAGATGGCTTCATTGGAATAGGAATTACTGCTGGTGCTATTATAGCAGCAGGTGGGATTGTAGCTACATGCCTTGGCTACCAGGCAGCGCCACTGTCCCAATCGTAG

Protein Analysis

181

Amino Acids

20.16

Weight (kDa)

7.67

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fis1_TPR_N PF14852 55 - 79 1.4e-08 Fis1 N-terminal tetratricopeptide repeat
Fis1_TPR_C PF14853 98 - 149 2.5e-20 Fis1 C-terminal tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0017619)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g07330
rosa_chinensis RchiOBHm_Chr5g0011611
rosa_laevigata RLG00000031846 RLG00000031848
rosa_multiflora Rmu_co8315781.1_g000001 Rmu_sc0009001.1_g000013
rosa_roxburghii Rroxscaffold_1G00064890
rosa_rugosa Rorug04G0458000
rosa_samantha Rh5BG085200 Rh5CG099100 Rh5DG085000
rosa_wichuraiana Rw5G007800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 479
AccI GTMKAC 1 cut(s) 140
AccIII TCCGGA 1 cut(s) 53
AclWI GGATC 2 cut(s) 294, 435
AcoI YGGCCR 2 cut(s) 261, 312
AcuI CTGAAG 1 cut(s) 431
AcyI GRCGYC 1 cut(s) 355
AfaI GTAC 1 cut(s) 271
AgsI TTSAA 3 cut(s) 258, 345, 383
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 1 cut(s) 503
AluI AGCT 1 cut(s) 503
Alw26I GTCTC 2 cut(s) 78, 388
AlwI GGATC 2 cut(s) 294, 435
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 2 cut(s) 261, 312
ApeKI GCWGC 2 cut(s) 485, 524
AspLEI GCGC 2 cut(s) 153, 529
AsuHPI GGTGA 2 cut(s) 146, 347
BaeGI GKGCMC 1 cut(s) 187
BalI TGGCCA 1 cut(s) 263
BanII GRGCYC 1 cut(s) 403
BarI GAAGNNNNNNTAC 2 cut(s) 51, 83
BbsI GAAGAC 3 cut(s) 66, 350, 422
BbvI GCAGC 2 cut(s) 497, 536
BccI CCATC 1 cut(s) 437
BceAI ACGGC 2 cut(s) 299, 327
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 314
BcoDI GTCTC 2 cut(s) 78, 388
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 479
BfuI GTATCC 1 cut(s) 314
BisI GCNGC 2 cut(s) 486, 525
BlsI GCNGC 2 cut(s) 487, 526
BmcAI AGTACT 1 cut(s) 271
Bme1390I CCNGG 1 cut(s) 521
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 1 cut(s) 288
BpiI GAAGAC 3 cut(s) 66, 350, 422
BplI GAGNNNNNCTC 2 cut(s) 385, 417
Bpu10I CCTNAGC 1 cut(s) 373
BsaHI GRCGYC 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 71, 511
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bse118I RCCGGY 1 cut(s) 351
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 2 cut(s) 71, 511
BseGI GGATG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 187
BseX3I CGGCCG 1 cut(s) 312
BseXI GCAGC 2 cut(s) 497, 536
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 2 cut(s) 263, 314
BsiEI CGRYCG 1 cut(s) 315
BsiSI CCGG 2 cut(s) 54, 352
BslFI GGGAC 1 cut(s) 520
BsmAI GTCTC 2 cut(s) 78, 388
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 520
BsnI GGCC 2 cut(s) 263, 314
Bsp1286I GDGCHC 2 cut(s) 187, 403
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 2 cut(s) 286, 427
Bsp19I CCATGG 1 cut(s) 71
BspANI GGCC 2 cut(s) 263, 314
BspCNI CTCAG 1 cut(s) 396
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 2 cut(s) 294, 435
BsrFI RCCGGY 1 cut(s) 351
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 2 cut(s) 71, 511
BssMI GATC 2 cut(s) 286, 427
BssNI GRCGYC 1 cut(s) 355
BssT1I CCWWGG 2 cut(s) 71, 511
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 2 cut(s) 421, 534
BstACI GRCGYC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 373, 404
BstDSI CCRYGG 1 cut(s) 71
BstF5I GGATG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 2 cut(s) 153, 529
BstKTI GATC 2 cut(s) 289, 430
BstMAI GTCTC 2 cut(s) 78, 388
BstMBI GATC 2 cut(s) 286, 427
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 2 cut(s) 235, 510
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 325
BstSLI GKGCMC 1 cut(s) 187
BstV1I GCAGC 2 cut(s) 497, 536
BstV2I GAAGAC 3 cut(s) 66, 350, 422
BstZI CGGCCG 1 cut(s) 312
BsuI GTATCC 1 cut(s) 314
BsuRI GGCC 2 cut(s) 263, 314
BtgI CCRYGG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 90
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 129, 530
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 1 cut(s) 353
CfoI GCGC 2 cut(s) 153, 529
Cfr10I RCCGGY 1 cut(s) 351
CseI GACGC 1 cut(s) 344
Csp6I GTAC 1 cut(s) 270
CviAII CATG 4 cut(s) 72, 180, 232, 507
CviJI RGCY 8 cut(s) 51, 263, 314, 351, 401, 447, 503, 516
CviKI_1 RGCY 8 cut(s) 51, 263, 314, 351, 401, 447, 503, 516
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 2 cut(s) 373, 404
DpnI GATC 2 cut(s) 288, 429
DpnII GATC 2 cut(s) 286, 427
EaeI YGGCCR 2 cut(s) 261, 312
EagI CGGCCG 1 cut(s) 312
EclXI CGGCCG 1 cut(s) 312
Eco130I CCWWGG 2 cut(s) 71, 511
Eco24I GRGCYC 1 cut(s) 403
Eco52I CGGCCG 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 431
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 2 cut(s) 71, 511
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 71, 511
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 4 cut(s) 75, 183, 235, 510
FaiI YATR 9 cut(s) 66, 68, 73, 96, 167, 181, 233, 482, 508
FaqI GGGAC 1 cut(s) 520
FatI CATG 4 cut(s) 71, 179, 231, 506
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 2 cut(s) 486, 525
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 486, 525
FspBI CTAG 1 cut(s) 348
GlaI GCGC 2 cut(s) 152, 528
GluI GCNGC 2 cut(s) 486, 525
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 2 cut(s) 263, 314
HapII CCGG 2 cut(s) 54, 352
HgaI GACGC 1 cut(s) 344
HhaI GCGC 2 cut(s) 153, 529
Hin1I GRCGYC 1 cut(s) 355
Hin1II CATG 4 cut(s) 75, 183, 235, 510
Hin6I GCGC 2 cut(s) 151, 527
HinP1I GCGC 2 cut(s) 151, 527
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HinfI GANTC 4 cut(s) 29, 137, 364, 406
HpaII CCGG 2 cut(s) 54, 352
HphI GGTGA 2 cut(s) 146, 347
Hpy166II GTNNAC 3 cut(s) 127, 141, 193
Hpy188I TCNGA 3 cut(s) 363, 405, 411
Hpy188III TCNNGA 2 cut(s) 54, 83
Hpy8I GTNNAC 3 cut(s) 127, 141, 193
HpyCH4III ACNGT 2 cut(s) 421, 534
HpyCH4IV ACGT 2 cut(s) 205, 220
HpyCH4V TGCA 3 cut(s) 78, 202, 217
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 2 cut(s) 373, 404
HpySE526I ACGT 2 cut(s) 205, 220
Hsp92I GRCGYC 1 cut(s) 355
Hsp92II CATG 4 cut(s) 75, 183, 235, 510
HspAI GCGC 2 cut(s) 151, 527
Kpn2I TCCGGA 1 cut(s) 53
KroI GCCGGC 1 cut(s) 351
KroNI GCCGGC 1 cut(s) 353
Kzo9I GATC 2 cut(s) 286, 427
LmnI GCTCC 2 cut(s) 56, 148
LpnPI CCDG 6 cut(s) 67, 365, 456, 474, 506, 533
Lsp1109I GCAGC 2 cut(s) 497, 536
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 348
MaeII ACGT 2 cut(s) 205, 220
MalI GATC 2 cut(s) 288, 429
MboI GATC 2 cut(s) 286, 427
MboII GAAGA 5 cut(s) 71, 221, 350, 424, 427
MhlI GDGCHC 2 cut(s) 187, 403
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 4 cut(s) 225, 253, 432, 462
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 3 cut(s) 23, 146, 415
MmeI TCCRAC 2 cut(s) 94, 298
MnlI CCTC 4 cut(s) 112, 139, 165, 418
Mox20I TGGCCA 1 cut(s) 263
MroI TCCGGA 1 cut(s) 53
MroNI GCCGGC 1 cut(s) 351
MscI TGGCCA 1 cut(s) 263
MslI CAYNNNNRTG 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 263
MspI CCGG 2 cut(s) 54, 352
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 1 cut(s) 482
NaeI GCCGGC 1 cut(s) 353
NcoI CCATGG 1 cut(s) 71
NdeII GATC 2 cut(s) 286, 427
NgoMIV GCCGGC 1 cut(s) 351
NlaIII CATG 4 cut(s) 75, 183, 235, 510
NlaIV GGNNCC 1 cut(s) 52
NspI RCATGY 2 cut(s) 235, 510
PaqCI CACCTGC 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 364
PflFI GACNNNGTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 487, 526
PleI GAGTC 3 cut(s) 23, 145, 414
PpsI GAGTC 3 cut(s) 23, 145, 414
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 52
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
RseI CAYNNNNRTG 1 cut(s) 99
SalI GTCGAC 1 cut(s) 139
SatI GCNGC 2 cut(s) 486, 525
Sau3AI GATC 2 cut(s) 286, 427
ScaI AGTACT 1 cut(s) 271
SchI GAGTC 3 cut(s) 23, 146, 415
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 2 cut(s) 187, 403
SetI ASST 8 cut(s) 101, 136, 176, 208, 223, 309, 493, 505
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 325
SmiMI CAYNNNNRTG 1 cut(s) 99
Sse9I AATT 4 cut(s) 225, 253, 432, 462
SspI AATATT 1 cut(s) 341
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 2 cut(s) 71, 511
TaaI ACNGT 2 cut(s) 421, 534
TaiI ACGT 2 cut(s) 208, 223
TaqI TCGA 3 cut(s) 82, 140, 430
TasI AATT 4 cut(s) 225, 253, 432, 462
TatI WGTACW 1 cut(s) 269
TfiI GAWTC 1 cut(s) 364
TscAI CASTG 2 cut(s) 129, 537
TseI GCWGC 2 cut(s) 485, 524
TspDTI ATGAA 3 cut(s) 17, 29, 439
TspGWI ACGGA 1 cut(s) 22
TspRI CASTG 2 cut(s) 129, 537
Tth111I GACNNNGTC 1 cut(s) 32
XceI RCATGY 2 cut(s) 235, 510
XmiI GTMKAC 1 cut(s) 140
XspI CTAG 1 cut(s) 348
ZrmI AGTACT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.