RLG00000032974

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011913
Physical Location & Seq
Reverse (-)
21598616 .. 21599896
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032974

Sequence Viewer

Length: 1281 bp
ATGGCCTCCGCTTGCGTCAACAACATCGGCATGTCGCCGGAGAAATTCGCGCCGGCGACCTACCCCTCCTACGGCTGGCTCAGCCCGAGAGTCTCCTTCAGCTGCGATAACACCGGCGATGGTGGCCGCCTTCCCGGAGCTAAGTCCTCGTCTCCGGCGAAGAATCAACCGGAAGTTCCAGATCCGGAGCTTTCTGGAGGAGAGTTCGAGTTCCGGCTGGAAGATCCGGTCGCCATGCTGCCCGCCGATGAACTCTTCTCCGGCGGGAAGCTCATGCCGCTCCACATCTCCTCCGTCAAACCTCCCGTCAACGAGCCCTCGTCTTCCGAGATCAGATCGCCGGAGACGACTAAGCACCGCCGGAGACAGGAGATTTCTGTTTCTGACCCGTACGTGTTCTCTCCCAAAGCTCCGAGGTGCTCGAGCCGTTGGAGAGAGCTATTGGGATTGAAGAAGCTGTACCAGAATTCCTCCAACGGTAACGGTAACGGCGGCGCGAAGGCCGAGACCAGTCAGAAAACGACGTCGTGCAGCACCTCTGCAAACGCGGCGCCCAAATCACTGAAGCACTTCCTTCACAGAAGCTCGAGATCGTTGTCGTCGTCGTCGTCGTCATCCTCCGACGCCTCGCTGAGCTTGCCTCTGCTCAAAGACTCCGACTGCGAGTCCGTCTCCATCTCCTCGCGCCTCTCGCTCTCGTCCTCGTCTTCGGGGCACGAGCACGACGACCTGCCTCGGCTCTCCCTCGATTCGGACAAACCGAATCCGAACCCGAACCCGATGTTCTCCCTCCACCGCAAGCCGGCGCGGGCCAAAACGGCCGTCAAGATTCGAGAAATCAAGCAGAGAGCGGCGGCGGCGGATCAGGCGGCGAGGGTGGGACGGAGCCCGATGAGGAGGCCGGCGGCTGAACCCGCCACCGTGGCGACCAGCAGAGGCGTCTCGGTGGATAGCCCGAGAATGAACTCGTCGGGGAAAATAGTGTTCCAAAGCTTAGAGCGGAGCTCGAGCAGTCCAAGCAGCTTCAACGGTGGGCCCCGGCTGAAGCACAGAGGAATGGAGCGGTCGTACTCAGCCAACGTCAGAGTGACTCCGGTTCTGAACGTCCCGGTCTGCTCCCTCCGGGGCTCCTCCAAATCCGGGTTTGGATTCGGGCAGCTATTCTCTTCTCCTCAGAAACGAGACTCAAACGGCGCAGTTTCCGGGAAAGGCGGCCAGCACAGCCATTACGTTCAACAGAGTCAGAGTCATAACAACAAAAACCGGACCGACCGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

427

Amino Acids

45.68

Weight (kDa)

9.99

Isoelectric Point (pI)

71.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56020 AT1G79060 AT3G12970
fragaria_vesca FvH4_3g16010
malus_domestica MD05G1215900.v1.1 MD10G1198500.v1.1
prunus_persica Prupe.4G143300_v2.0.a1
pyrus_communis pycom05g19860 pycom10g17120
rosa_chinensis RchiOBHm_Chr5g0026971
rosa_laevigata RLG00000032974
rosa_multiflora Rmu_sc0002742.1_g000001
rosa_roxburghii Rroxscaffold_1G00052510
rosa_rugosa Rorug05G0096400
rosa_samantha Rh5AG189800 Rh5BG187300 Rh5CG207000 Rh5DG189000
rosa_wichuraiana Rw5G017270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 527
Acc36I ACCTGC 1 cut(s) 738
AccB1I GGYRCC 1 cut(s) 550
AccBSI CCGCTC 4 cut(s) 280, 851, 1000, 1063
AccII CGCG 5 cut(s) 50, 497, 548, 685, 808
AccIII TCCGGA 1 cut(s) 184
AclWI GGATC 3 cut(s) 176, 218, 870
AcoI YGGCCR 3 cut(s) 124, 819, 1213
AcsI RAATTY 2 cut(s) 44, 466
AcuI CTGAAG 3 cut(s) 82, 584, 1064
AcyI GRCGYC 4 cut(s) 524, 551, 624, 939
AfaI GTAC 3 cut(s) 392, 461, 1070
AfiI CCNNNNNNNGG 6 cut(s) 71, 75, 367, 751, 802, 1140
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 451, 1027, 1235
AhdI GACNNNNNGTC 1 cut(s) 664
Alw21I GWGCWC 3 cut(s) 422, 723, 1007
Alw26I GTCTC 8 cut(s) 97, 156, 338, 358, 500, 676, 946, 1176
AlwI GGATC 3 cut(s) 176, 218, 870
Ama87I CYCGRG 5 cut(s) 85, 421, 586, 955, 1006
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 8 cut(s) 3, 124, 501, 810, 819, 899, 1034, 1213
ApaI GGGCCC 1 cut(s) 1038
ApeKI GCWGC 5 cut(s) 102, 238, 531, 1020, 1156
ApoI RAATTY 2 cut(s) 44, 466
ArsI GACNNNNNNTTYG 4 cut(s) 291, 323, 807, 839
Asp700I GAANNNNTTC 1 cut(s) 569
AspLEI GCGC 6 cut(s) 52, 497, 553, 687, 808, 1196
AspS9I GGNCC 4 cut(s) 810, 1034, 1035, 1266
AsuC2I CCSGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
AvaI CYCGRG 5 cut(s) 85, 421, 586, 955, 1006
AvaII GGWCC 1 cut(s) 1266
BaeGI GKGCMC 2 cut(s) 717, 1038
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 5 cut(s) 318, 890, 1007, 1038, 1130
BarI GAAGNNNNNNTAC 2 cut(s) 443, 475
BauI CACGAG 1 cut(s) 716
BbsI GAAGAC 2 cut(s) 315, 699
Bbv12I GWGCWC 3 cut(s) 422, 723, 1007
BbvI GCAGC 5 cut(s) 89, 225, 543, 1032, 1168
BccI CCATC 2 cut(s) 113, 683
BceAI ACGGC 6 cut(s) 88, 411, 505, 806, 834, 1207
BcnI CCSGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
BcoDI GTCTC 8 cut(s) 97, 156, 338, 358, 500, 676, 946, 1176
BfoI RGCGCY 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 738
BglI GCCNNNNNGGC 2 cut(s) 818, 923
BlpI GCTNAGC 2 cut(s) 80, 632
Bme1390I CCNGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
Bme18I GGWCC 1 cut(s) 1266
BmeRI GACNNNNNGTC 1 cut(s) 664
BmeT110I CYCGRG 5 cut(s) 85, 421, 586, 955, 1006
BmgT120I GGNCC 4 cut(s) 810, 1034, 1035, 1266
BmiI GGNNCC 5 cut(s) 552, 887, 1036, 1037, 1129
BmrFI CCNGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
BpiI GAAGAC 2 cut(s) 315, 699
BplI GAGNNNNNCTC 6 cut(s) 625, 657, 656, 688, 989, 1021
BpmI CTGGAG 1 cut(s) 216
Bpu1102I GCTNAGC 2 cut(s) 80, 632
BpuMI CCSGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 4 cut(s) 524, 551, 624, 939
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 5 cut(s) 413, 734, 921, 1037, 1123
BsaWI WCCGGW 5 cut(s) 169, 184, 226, 1093, 1263
BsaXI ACNNNNNCTCC 4 cut(s) 275, 305, 1052, 1082
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 75, 367, 751, 802, 1140
Bse118I RCCGGY 4 cut(s) 52, 113, 802, 901
Bse1I ACTGG 1 cut(s) 510
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 5 cut(s) 413, 734, 921, 1037, 1123
BseGI GGATG 1 cut(s) 614
BseLI CCNNNNNNNGG 6 cut(s) 71, 75, 367, 751, 802, 1140
BseMII CTCAG 4 cut(s) 94, 623, 1086, 1187
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 6 cut(s) 213, 280, 670, 910, 1120, 1161
BseSI GKGCMC 2 cut(s) 717, 1038
BseX3I CGGCCG 1 cut(s) 819
BseXI GCAGC 5 cut(s) 89, 225, 543, 1032, 1168
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 5 cut(s) 50, 497, 548, 685, 808
Bsh1285I CGRYCG 4 cut(s) 231, 822, 1067, 1273
BshFI GGCC 8 cut(s) 5, 126, 503, 812, 821, 901, 1036, 1215
BshNI GGYRCC 1 cut(s) 550
BsiEI CGRYCG 4 cut(s) 231, 822, 1067, 1273
BsiHKAI GWGCWC 3 cut(s) 422, 723, 1007
BsiHKCI CYCGRG 5 cut(s) 85, 421, 586, 955, 1006
BsiWI CGTACG 1 cut(s) 390
BslFI GGGAC 2 cut(s) 894, 1091
BslI CCNNNNNNNGG 6 cut(s) 71, 75, 367, 751, 802, 1140
BsmAI GTCTC 8 cut(s) 97, 156, 338, 358, 500, 676, 946, 1176
BsmBI CGTCTC 4 cut(s) 156, 338, 676, 946
BsmFI GGGAC 2 cut(s) 894, 1091
BsnI GGCC 8 cut(s) 5, 126, 503, 812, 821, 901, 1036, 1215
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 5 cut(s) 85, 421, 586, 955, 1006
Bsp120I GGGCCC 1 cut(s) 1034
Bsp1286I GDGCHC 8 cut(s) 318, 422, 717, 723, 890, 1007, 1038, 1130
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 6 cut(s) 181, 223, 330, 335, 590, 862
Bsp1720I GCTNAGC 2 cut(s) 80, 632
BspANI GGCC 8 cut(s) 5, 126, 503, 812, 821, 901, 1036, 1215
BspCNI CTCAG 4 cut(s) 93, 624, 1085, 1186
BspEI TCCGGA 1 cut(s) 184
BspFNI CGCG 5 cut(s) 50, 497, 548, 685, 808
BspLI GGNNCC 5 cut(s) 552, 887, 1036, 1037, 1129
BspMI ACCTGC 1 cut(s) 738
BspPI GGATC 3 cut(s) 176, 218, 870
BspT107I GGYRCC 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 4 cut(s) 280, 851, 1000, 1063
BsrFI RCCGGY 4 cut(s) 52, 113, 802, 901
BsrI ACTGG 1 cut(s) 510
BssAI RCCGGY 4 cut(s) 52, 113, 802, 901
BssECI CCNNGG 5 cut(s) 413, 734, 921, 1037, 1123
BssMI GATC 6 cut(s) 181, 223, 330, 335, 590, 862
BssNI GRCGYC 4 cut(s) 524, 551, 624, 939
BssSI CACGAG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 716
Bst4CI ACNGT 5 cut(s) 479, 485, 922, 1031, 1274
Bst6I CTCTTC 2 cut(s) 260, 1171
BstACI GRCGYC 4 cut(s) 524, 551, 624, 939
BstBAI YACGTR 1 cut(s) 394
BstDEI CTNAG 7 cut(s) 80, 141, 351, 632, 994, 1072, 1173
BstDSI CCRYGG 1 cut(s) 921
BstF5I GGATG 1 cut(s) 614
BstFNI CGCG 5 cut(s) 50, 497, 548, 685, 808
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 6 cut(s) 52, 497, 553, 687, 808, 1196
BstKTI GATC 6 cut(s) 184, 226, 333, 338, 593, 865
BstMAI GTCTC 8 cut(s) 97, 156, 338, 358, 500, 676, 946, 1176
BstMBI GATC 6 cut(s) 181, 223, 330, 335, 590, 862
BstMCI CGRYCG 4 cut(s) 231, 822, 1067, 1273
BstNSI RCATGY 1 cut(s) 34
BstSCI CCNGG 6 cut(s) 133, 1037, 1107, 1122, 1139, 1202
BstSLI GKGCMC 2 cut(s) 717, 1038
BstUI CGCG 5 cut(s) 50, 497, 548, 685, 808
BstV1I GCAGC 5 cut(s) 89, 225, 543, 1032, 1168
BstV2I GAAGAC 2 cut(s) 315, 699
BstX2I RGATCY 2 cut(s) 181, 223
BstYI RGATCY 2 cut(s) 181, 223
BstZI CGGCCG 1 cut(s) 819
BsuRI GGCC 8 cut(s) 5, 126, 503, 812, 821, 901, 1036, 1215
BtgI CCRYGG 1 cut(s) 921
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 1 cut(s) 614
BtsIMutI CAGTG 1 cut(s) 560
BveI ACCTGC 1 cut(s) 738
CfoI GCGC 6 cut(s) 52, 497, 553, 687, 808, 1196
Cfr10I RCCGGY 4 cut(s) 52, 113, 802, 901
Cfr13I GGNCC 4 cut(s) 810, 1034, 1035, 1266
CpoI CGGWCCG 1 cut(s) 1266
CseI GACGC 3 cut(s) 4, 632, 928
Csp6I GTAC 3 cut(s) 391, 460, 1069
CspI CGGWCCG 1 cut(s) 1266
CviAII CATG 3 cut(s) 31, 235, 274
CviQI GTAC 3 cut(s) 391, 460, 1069
DdeI CTNAG 7 cut(s) 80, 141, 351, 632, 994, 1072, 1173
DinI GGCGCC 1 cut(s) 552
DpnI GATC 6 cut(s) 183, 225, 332, 337, 592, 864
DpnII GATC 6 cut(s) 181, 223, 330, 335, 590, 862
DriI GACNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 3 cut(s) 124, 819, 1213
EagI CGGCCG 1 cut(s) 819
Eam1104I CTCTTC 2 cut(s) 260, 1171
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 2 cut(s) 260, 1171
EciI GGCGGA 1 cut(s) 875
Ecl136II GAGCTC 1 cut(s) 1005
EclXI CGGCCG 1 cut(s) 819
Eco24I GRGCYC 5 cut(s) 318, 890, 1007, 1038, 1130
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 1266
Eco52I CGGCCG 1 cut(s) 819
Eco53kI GAGCTC 1 cut(s) 1005
Eco57I CTGAAG 3 cut(s) 82, 584, 1064
Eco88I CYCGRG 5 cut(s) 85, 421, 586, 955, 1006
EcoICRI GAGCTC 1 cut(s) 1005
EcoO109I RGGNCCY 1 cut(s) 1035
EcoRI GAATTC 1 cut(s) 466
EcoT38I GRGCYC 5 cut(s) 318, 890, 1007, 1038, 1130
EgeI GGCGCC 1 cut(s) 552
EheI GGCGCC 1 cut(s) 552
Esp3I CGTCTC 4 cut(s) 156, 338, 676, 946
FaeI CATG 3 cut(s) 34, 238, 277
FaiI YATR 4 cut(s) 32, 236, 275, 1251
FaqI GGGAC 2 cut(s) 894, 1091
FatI CATG 3 cut(s) 30, 234, 273
FauI CCCGC 4 cut(s) 250, 257, 801, 922
FokI GGATG 1 cut(s) 601
FriOI GRGCYC 5 cut(s) 318, 890, 1007, 1038, 1130
GlaI GCGC 6 cut(s) 51, 496, 552, 686, 807, 1195
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 8 cut(s) 5, 126, 503, 812, 821, 901, 1036, 1215
HgaI GACGC 3 cut(s) 4, 632, 928
HhaI GCGC 6 cut(s) 52, 497, 553, 687, 808, 1196
Hin1I GRCGYC 4 cut(s) 524, 551, 624, 939
Hin1II CATG 3 cut(s) 34, 238, 277
Hin6I GCGC 6 cut(s) 50, 495, 551, 685, 806, 1194
HinP1I GCGC 6 cut(s) 50, 495, 551, 685, 806, 1194
HincII GTYRAC 2 cut(s) 19, 310
HindII GTYRAC 2 cut(s) 19, 310
HindIII AAGCTT 1 cut(s) 991
Hpy166II GTNNAC 2 cut(s) 19, 310
Hpy188III TCNNGA 6 cut(s) 179, 185, 195, 588, 826, 833
Hpy8I GTNNAC 2 cut(s) 19, 310
Hpy99I CGWCG 9 cut(s) 526, 529, 604, 607, 610, 613, 626, 728, 973
HpyAV CCTTC 4 cut(s) 106, 140, 493, 584
HpyCH4III ACNGT 5 cut(s) 479, 485, 922, 1031, 1274
HpyCH4IV ACGT 5 cut(s) 393, 524, 1080, 1104, 1230
HpyCH4V TGCA 2 cut(s) 531, 542
HpyF3I CTNAG 7 cut(s) 80, 141, 351, 632, 994, 1072, 1173
HpySE526I ACGT 5 cut(s) 393, 524, 1080, 1104, 1230
Hsp92I GRCGYC 4 cut(s) 524, 551, 624, 939
Hsp92II CATG 3 cut(s) 34, 238, 277
HspAI GCGC 6 cut(s) 50, 495, 551, 685, 806, 1194
KasI GGCGCC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 184
KroI GCCGGC 3 cut(s) 52, 802, 901
KroNI GCCGGC 3 cut(s) 54, 804, 903
Kzo9I GATC 6 cut(s) 181, 223, 330, 335, 590, 862
LmnI GCTCC 9 cut(s) 137, 187, 285, 415, 885, 1002, 1060, 1121, 1133
Lsp1109I GCAGC 5 cut(s) 89, 225, 543, 1032, 1168
MaeII ACGT 5 cut(s) 393, 524, 1080, 1104, 1230
MaeIII GTNAC 4 cut(s) 479, 485, 1087, 1274
MalI GATC 6 cut(s) 183, 225, 332, 337, 592, 864
MbiI CCGCTC 4 cut(s) 280, 851, 1000, 1063
MboI GATC 6 cut(s) 181, 223, 330, 335, 590, 862
MboII GAAGA 7 cut(s) 172, 233, 247, 315, 463, 699, 1158
MflI RGATCY 2 cut(s) 181, 223
MhlI GDGCHC 8 cut(s) 318, 422, 717, 723, 890, 1007, 1038, 1130
MluCI AATT 2 cut(s) 44, 466
Mly113I GGCGCC 1 cut(s) 551
MlyI GAGTC 7 cut(s) 99, 647, 674, 1084, 1178, 1249, 1255
MmeI TCCRAC 4 cut(s) 410, 498, 645, 681
MreI CGCCGGCG 1 cut(s) 52
MroI TCCGGA 1 cut(s) 184
MroNI GCCGGC 3 cut(s) 52, 802, 901
MroXI GAANNNNTTC 1 cut(s) 569
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 102
MspR9I CCNGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
MvnI CGCG 5 cut(s) 50, 497, 548, 685, 808
NaeI GCCGGC 3 cut(s) 54, 804, 903
NarI GGCGCC 1 cut(s) 551
NciI CCSGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
NdeII GATC 6 cut(s) 181, 223, 330, 335, 590, 862
NgoMIV GCCGGC 3 cut(s) 52, 802, 901
NlaIII CATG 3 cut(s) 34, 238, 277
NlaIV GGNNCC 5 cut(s) 552, 887, 1036, 1037, 1129
NmeAIII GCCGAG 2 cut(s) 529, 715
NmuCI GTSAC 1 cut(s) 1087
NspI RCATGY 1 cut(s) 34
PaeR7I CTCGAG 3 cut(s) 421, 586, 1006
PcsI WCGNNNNNNNCGW 7 cut(s) 155, 344, 599, 605, 608, 723, 758
PdiI GCCGGC 3 cut(s) 54, 804, 903
PdmI GAANNNNTTC 1 cut(s) 569
PfeI GAWTC 5 cut(s) 163, 749, 763, 829, 1149
Pfl23II CGTACG 1 cut(s) 390
PfoI TCCNGGA 2 cut(s) 133, 1202
PleI GAGTC 7 cut(s) 98, 647, 673, 1084, 1178, 1248, 1254
PluTI GGCGCC 1 cut(s) 554
PpsI GAGTC 7 cut(s) 98, 647, 673, 1084, 1178, 1248, 1254
Ppu21I YACGTR 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 1007
PspLI CGTACG 1 cut(s) 390
PspN4I GGNNCC 5 cut(s) 552, 887, 1036, 1037, 1129
PspOMI GGGCCC 1 cut(s) 1034
PspPI GGNCC 4 cut(s) 810, 1034, 1035, 1266
PspXI VCTCGAGB 2 cut(s) 421, 1006
PsuI RGATCY 2 cut(s) 181, 223
PvuII CAGCTG 1 cut(s) 102
RsaI GTAC 3 cut(s) 392, 461, 1070
RsaNI GTAC 3 cut(s) 391, 460, 1069
RseI CAYNNNNRTG 1 cut(s) 29
Rsr2I CGGWCCG 1 cut(s) 1266
RsrII CGGWCCG 1 cut(s) 1266
SacI GAGCTC 1 cut(s) 1007
Sau3AI GATC 6 cut(s) 181, 223, 330, 335, 590, 862
Sau96I GGNCC 4 cut(s) 810, 1034, 1035, 1266
SchI GAGTC 7 cut(s) 99, 647, 674, 1084, 1178, 1249, 1255
ScrFI CCNGG 6 cut(s) 135, 1039, 1109, 1124, 1141, 1204
SduI GDGCHC 8 cut(s) 318, 422, 717, 723, 890, 1007, 1038, 1130
SfiI GGCCNNNNNGGCC 1 cut(s) 818
SfoI GGCGCC 1 cut(s) 552
Sfr274I CTCGAG 3 cut(s) 421, 586, 1006
SgrAI CRCCGGYG 2 cut(s) 52, 113
SinI GGWCC 1 cut(s) 1266
SlaI CTCGAG 3 cut(s) 421, 586, 1006
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 3 cut(s) 421, 586, 1006
SmoI CTYRAG 3 cut(s) 421, 586, 1006
Sse9I AATT 2 cut(s) 44, 466
SspDI GGCGCC 1 cut(s) 550
SstI GAGCTC 1 cut(s) 1007
StyD4I CCNGG 6 cut(s) 133, 1037, 1107, 1122, 1139, 1202
TaaI ACNGT 5 cut(s) 479, 485, 922, 1031, 1274
TaiI ACGT 5 cut(s) 396, 527, 1083, 1107, 1233
TaqI TCGA 6 cut(s) 207, 422, 587, 747, 832, 1007
TasI AATT 2 cut(s) 44, 466
TfiI GAWTC 5 cut(s) 163, 749, 763, 829, 1149
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 5 cut(s) 102, 238, 531, 1020, 1156
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 2 cut(s) 264, 977
TspGWI ACGGA 3 cut(s) 283, 658, 898
TspRI CASTG 1 cut(s) 567
VpaK11BI GGWCC 1 cut(s) 1266
XapI RAATTY 2 cut(s) 44, 466
XceI RCATGY 1 cut(s) 34
XhoI CTCGAG 3 cut(s) 421, 586, 1006
XmnI GAANNNNTTC 1 cut(s) 569
ZraI GACGTC 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.