RLG00000034789

SPRY domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011366
Physical Location & Seq
Reverse (-)
54589663 .. 54592357
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034789

Sequence Viewer

Length: 1326 bp
ATGGACAATCTCCAATCCAATTACATGGACGAAGACGAGGAAGAGGACGAACAATCCAAAACCACCACCGCCGCCGCCGTCGCAATCACCGAGCTCGTTGAGCCCGCGACGACAACGGAGCCCGCAAACGACGACGAGCCCAATTCGGAGCCCATGATTAACCCACCGCCCACCGACCCGCTCAACGGCAACAAAACCGAGACCGAAGCCTCCGAAGACCTCTCCGAGTCCGTCAAATCCCTCGAAAACGACGTCGACGTCGACATCGAAGACGAAGACCCGCATCCCAAGAAGCAAAAGCAGCTCTCTTCTCTCACCGCCATTGCCGCGGCGACCACCCCACCCGCTTCTTCGGACCCTGACCAGGCCCAATTGCCCCAACCCGCACCCGAAGCCAACGGCAACGGCAACGGCGCTCTCCTCCTCCCGGCGCAACCCTCCAAGAAGTCGACCAAGAAGAAGAACAACAATGTGTGGGTCACCAAGTCGACCCGGAAAGGGAAGAAGAAGACCAAGCCCAGCACCAACAACAATGCCCCCGGAGAGGACAAGGTTCTCGTCACTCCGGTCACCAGATTCCCCGACAAAACCGACGACACGCCGGAGATGACTATCTGCCTCTCCAAGGTCTACAAGGCCGAGAAAGTTGAGGTCGGCGAGGACCGAATGAGCGCCGGGAGTACTAAAGGGTATAGAATGGTTAGGGCCACCAGGGGAGTTGTGGAAGGTGCTTGGTACTTTGAAATTAGGGTAGTCAGCTTGGGAGAGACTGGCCACACCCGGCTTGGCTGGTCCACTGACAAAGGTGACCTTCAGGCTCCGGTTGGATATGATGGGAATAGTTTCGGGTATCGGGACATTGATGGGAGTAAGGTGCATAAGGCTTTGAGGGAGAAGTATGGGGAGGAAGGGTATAAGGAAGGTGATGTTATAGGGTTCTATATAAATTTGCCGGATGGGGGGTCTTATGCCCCCAAACCTCCGCATTTGGTTTGGTATAAGGGGCAGAGGTATGCTACAGCACCTGAAGCCAAGGAGGATGCTGTCAAAGTAGTGCCTGGAAGTGAGATATCTTTTTTCAAAAATGGGGTATGCCAAGGTGTTGCTTTTAAAGATCTATATGGTGGTCGTTACTATCCTGCTGCTTCAATGTATACTCTTCCCCATCAACCAAATTGTGTGGTCAAGTTCAACTTTGGTCCTGACTTTGAATTCTTTCCAGAAGACTTCAATGAACGTCCAGTGCCCAGGCCCATGGTTGAAGTTCCTTATCATGGGTTTGAAAACCGTGCTGAAAATGGAGTGTCCAATGAGAAGAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0002376 GO:0002520 GO:0002682 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006325 GO:0006351 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007275 GO:0008013 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008284 GO:0008757 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009965 GO:0009987 GO:0010016 GO:0010154 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016278 GO:0016279 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018023 GO:0018024 GO:0018130 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0022414 GO:0022607 GO:0030097 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034708 GO:0034968 GO:0035097 GO:0036211 GO:0042054 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0043627 GO:0043933 GO:0044085 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044666 GO:0045595 GO:0045637 GO:0045652 GO:0046483 GO:0048188 GO:0048316 GO:0048366 GO:0048367 GO:0048513 GO:0048518 GO:0048522 GO:0048534 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051239 GO:0051252 GO:0051276 GO:0051568 GO:0051716 GO:0060255 GO:0060776 GO:0061458 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0080182 GO:0090304 GO:0097159 GO:0097659 GO:0099402 GO:0140096 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1903506 GO:1903706 GO:1904837 GO:1905392 GO:1990234 GO:2000026 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04212 ko04934 map04212 map04934
Pfam Domains
Protein Families
SPRY

Protein Analysis

442

Amino Acids

48.44

Weight (kDa)

4.9

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPRY PF00622 245 - 320 2.6e-09 SPRY domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51450
fragaria_vesca FvH4_3g28490 FvH4_3g28490 FvH4_3g28490
malus_domestica MD03G1172400.v1.1 MD11G1190300.v1.1
prunus_persica Prupe.4G254200_v2.0.a1
pyrus_communis pycom03g12960 pycom11g16680
rosa_chinensis RchiOBHm_Chr5g0052831
rosa_laevigata RLG00000034789
rosa_multiflora Rmu_ssc0000311.1_g000014
rosa_roxburghii Rroxscaffold_1G00028660
rosa_rugosa Rorug05G0269300 Rorug05G0269400
rosa_samantha Rh5AG341400 Rh5BG352100 Rh5CG378800 Rh5DG368400
rosa_wichuraiana Rw5G032190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AatII GACGTC 2 cut(s) 255, 261
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 6 cut(s) 255, 261, 449, 488, 630, 1154
AccII CGCG 2 cut(s) 107, 329
AcoI YGGCCR 1 cut(s) 772
AcsI RAATTY 2 cut(s) 946, 1211
AcuI CTGAAG 2 cut(s) 797, 1047
AcyI GRCGYC 2 cut(s) 252, 258
AfaI GTAC 2 cut(s) 682, 737
AfiI CCNNNNNNNGG 8 cut(s) 185, 364, 427, 498, 544, 625, 959, 1274
AgsI TTSAA 8 cut(s) 743, 1081, 1149, 1192, 1211, 1231, 1262, 1283
AjnI CCWGG 4 cut(s) 363, 710, 1057, 1247
AluBI AGCT 3 cut(s) 94, 304, 759
AluI AGCT 3 cut(s) 94, 304, 759
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 194, 761
AlwNI CAGNNNCTG 1 cut(s) 1025
AoxI GGCC 5 cut(s) 366, 636, 705, 772, 1250
ApeKI GCWGC 2 cut(s) 301, 1142
ApoI RAATTY 2 cut(s) 946, 1211
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
Asp700I GAANNNNTTC 2 cut(s) 842, 1215
AspLEI GCGC 3 cut(s) 416, 433, 674
AspS9I GGNCC 7 cut(s) 355, 367, 661, 705, 792, 1199, 1251
AsuC2I CCSGG 5 cut(s) 428, 493, 540, 676, 781
AsuHPI GGTGA 6 cut(s) 79, 307, 472, 562, 818, 935
AvaII GGWCC 4 cut(s) 355, 661, 792, 1199
BaeGI GKGCMC 1 cut(s) 1248
BalI TGGCCA 1 cut(s) 774
BanII GRGCYC 5 cut(s) 96, 105, 123, 141, 153
BbsI GAAGAC 6 cut(s) 39, 222, 276, 282, 515, 1230
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 313, 1129
BccI CCATC 4 cut(s) 827, 857, 950, 1173
BceAI ACGGC 5 cut(s) 62, 202, 415, 421, 427
BciT130I CCWGG 4 cut(s) 365, 712, 1059, 1249
BcnI CCSGG 5 cut(s) 428, 493, 540, 676, 781
BcoDI GTCTC 2 cut(s) 194, 761
BfmI CTRYAG 1 cut(s) 1017
BfoI RGCGCY 2 cut(s) 417, 675
BglII AGATCT 1 cut(s) 1114
BisI GCNGC 6 cut(s) 72, 75, 302, 327, 330, 1143
BlsI GCNGC 6 cut(s) 73, 76, 303, 328, 331, 1144
BmcAI AGTACT 1 cut(s) 682
Bme1390I CCNGG 9 cut(s) 365, 428, 493, 540, 676, 712, 781, 1059, 1249
Bme18I GGWCC 4 cut(s) 355, 661, 792, 1199
BmgT120I GGNCC 7 cut(s) 355, 367, 661, 705, 792, 1199, 1251
BmiI GGNNCC 4 cut(s) 120, 150, 357, 819
BmrFI CCNGG 9 cut(s) 365, 428, 493, 540, 676, 712, 781, 1059, 1249
BmsI GCATC 2 cut(s) 292, 1030
BpiI GAAGAC 6 cut(s) 39, 222, 276, 282, 515, 1230
BpuMI CCSGG 5 cut(s) 428, 493, 540, 676, 781
BsaBI GATNNNNATC 1 cut(s) 611
BsaHI GRCGYC 2 cut(s) 252, 258
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 8 cut(s) 327, 538, 624, 711, 1032, 1096, 1247, 1254
BsaWI WCCGGW 2 cut(s) 565, 820
BsaXI ACNNNNNCTCC 2 cut(s) 896, 926
Bsc4I CCNNNNNNNGG 8 cut(s) 185, 364, 427, 498, 544, 625, 959, 1274
Bse1I ACTGG 2 cut(s) 775, 1241
Bse3DI GCAATG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 611
BseBI CCWGG 4 cut(s) 365, 712, 1059, 1249
BseDI CCNNGG 8 cut(s) 327, 538, 624, 711, 1032, 1096, 1247, 1254
BseGI GGATG 3 cut(s) 283, 961, 1045
BseJI GATNNNNATC 1 cut(s) 611
BseLI CCNNNNNNNGG 8 cut(s) 185, 364, 427, 498, 544, 625, 959, 1274
BseMI GCAATG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 775, 1241
BseRI GAGGAG 2 cut(s) 410, 413
BseSI GKGCMC 1 cut(s) 1248
BseXI GCAGC 2 cut(s) 313, 1129
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 2 cut(s) 107, 329
BshFI GGCC 5 cut(s) 368, 638, 707, 774, 1252
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 9 cut(s) 428, 493, 540, 566, 602, 675, 781, 821, 953
BslFI GGGAC 1 cut(s) 869
BslI CCNNNNNNNGG 8 cut(s) 185, 364, 427, 498, 544, 625, 959, 1274
BsmAI GTCTC 2 cut(s) 194, 761
BsmFI GGGAC 1 cut(s) 869
BsnI GGCC 5 cut(s) 368, 638, 707, 774, 1252
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 6 cut(s) 96, 105, 123, 141, 153, 1248
Bsp143I GATC 1 cut(s) 1114
Bsp19I CCATGG 1 cut(s) 1254
BspANI GGCC 5 cut(s) 368, 638, 707, 774, 1252
BspFNI CGCG 2 cut(s) 107, 329
BspLI GGNNCC 4 cut(s) 120, 150, 357, 819
BspTNI GGTCTC 1 cut(s) 194
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 321
BsrI ACTGG 2 cut(s) 775, 1241
BssECI CCNNGG 8 cut(s) 327, 538, 624, 711, 1032, 1096, 1247, 1254
BssMI GATC 1 cut(s) 1114
BssNAI GTATAC 1 cut(s) 1155
BssNI GRCGYC 2 cut(s) 252, 258
BssT1I CCWWGG 4 cut(s) 624, 1032, 1096, 1254
Bst1107I GTATAC 1 cut(s) 1155
Bst2UI CCWGG 4 cut(s) 365, 712, 1059, 1249
Bst4CI ACNGT 1 cut(s) 1289
Bst6I CTCTTC 3 cut(s) 36, 313, 1164
BstACI GRCGYC 2 cut(s) 252, 258
BstC8I GCNNGC 2 cut(s) 105, 123
BstDSI CCRYGG 2 cut(s) 327, 1254
BstEII GGTNACC 3 cut(s) 478, 568, 806
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 3 cut(s) 283, 961, 1045
BstFNI CGCG 2 cut(s) 107, 329
BstH2I RGCGCY 2 cut(s) 417, 675
BstHHI GCGC 3 cut(s) 416, 433, 674
BstKTI GATC 1 cut(s) 1117
BstMAI GTCTC 2 cut(s) 194, 761
BstMBI GATC 1 cut(s) 1114
BstMWI GCNNNNNNNGC 6 cut(s) 80, 100, 301, 326, 392, 1028
BstNI CCWGG 4 cut(s) 365, 712, 1059, 1249
BstPI GGTNACC 3 cut(s) 478, 568, 806
BstSCI CCNGG 9 cut(s) 363, 426, 491, 538, 674, 710, 779, 1057, 1247
BstSFI CTRYAG 1 cut(s) 1017
BstSLI GKGCMC 1 cut(s) 1248
BstUI CGCG 2 cut(s) 107, 329
BstV1I GCAGC 2 cut(s) 313, 1129
BstV2I GAAGAC 6 cut(s) 39, 222, 276, 282, 515, 1230
BstX2I RGATCY 1 cut(s) 1114
BstXI CCANNNNNNTGG 2 cut(s) 25, 1255
BstYI RGATCY 1 cut(s) 1114
BstZ17I GTATAC 1 cut(s) 1155
BsuRI GGCC 5 cut(s) 368, 638, 707, 774, 1252
BtgI CCRYGG 2 cut(s) 327, 1254
BtsCI GGATG 3 cut(s) 283, 961, 1045
BtsIMutI CAGTG 2 cut(s) 795, 1248
Cac8I GCNNGC 2 cut(s) 105, 123
CaiI CAGNNNCTG 1 cut(s) 1025
CfoI GCGC 3 cut(s) 416, 433, 674
Cfr13I GGNCC 7 cut(s) 355, 367, 661, 705, 792, 1199, 1251
Cfr42I CCGCGG 1 cut(s) 330
Csp6I GTAC 2 cut(s) 681, 736
CspCI CAANNNNNGTGG 2 cut(s) 1161, 1196
CviAII CATG 4 cut(s) 25, 154, 1255, 1274
CviQI GTAC 2 cut(s) 681, 736
DpnI GATC 1 cut(s) 1116
DpnII GATC 1 cut(s) 1114
DraI TTTAAA 1 cut(s) 1111
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 1 cut(s) 772
Eam1104I CTCTTC 3 cut(s) 36, 313, 1164
EarI CTCTTC 3 cut(s) 36, 313, 1164
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 624, 1032, 1096, 1254
Eco24I GRGCYC 5 cut(s) 96, 105, 123, 141, 153
Eco31I GGTCTC 1 cut(s) 194
Eco32I GATATC 1 cut(s) 1071
Eco47I GGWCC 4 cut(s) 355, 661, 792, 1199
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 797, 1047
Eco91I GGTNACC 3 cut(s) 478, 568, 806
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoO65I GGTNACC 3 cut(s) 478, 568, 806
EcoRI GAATTC 1 cut(s) 1211
EcoRII CCWGG 4 cut(s) 363, 710, 1057, 1247
EcoRV GATATC 1 cut(s) 1071
EcoT14I CCWWGG 4 cut(s) 624, 1032, 1096, 1254
EcoT38I GRGCYC 5 cut(s) 96, 105, 123, 141, 153
ErhI CCWWGG 4 cut(s) 624, 1032, 1096, 1254
FaeI CATG 4 cut(s) 28, 157, 1258, 1277
FalI AAGNNNNNCTT 4 cut(s) 795, 827, 1178, 1210
FaqI GGGAC 1 cut(s) 869
FatI CATG 4 cut(s) 24, 153, 1254, 1273
FauI CCCGC 6 cut(s) 112, 130, 186, 288, 352, 391
FblI GTMKAC 6 cut(s) 255, 261, 449, 488, 630, 1154
Fnu4HI GCNGC 6 cut(s) 72, 75, 302, 327, 330, 1143
FokI GGATG 3 cut(s) 270, 968, 1052
FriOI GRGCYC 5 cut(s) 96, 105, 123, 141, 153
Fsp4HI GCNGC 6 cut(s) 72, 75, 302, 327, 330, 1143
GlaI GCGC 3 cut(s) 415, 432, 673
GluI GCNGC 6 cut(s) 72, 75, 302, 327, 330, 1143
GsaI CCCAGC 1 cut(s) 522
HaeII RGCGCY 2 cut(s) 417, 675
HaeIII GGCC 5 cut(s) 368, 638, 707, 774, 1252
HapII CCGG 9 cut(s) 428, 493, 540, 566, 602, 675, 781, 821, 953
HhaI GCGC 3 cut(s) 416, 433, 674
Hin1I GRCGYC 2 cut(s) 252, 258
Hin1II CATG 4 cut(s) 28, 157, 1258, 1277
Hin6I GCGC 3 cut(s) 414, 431, 672
HinP1I GCGC 3 cut(s) 414, 431, 672
HincII GTYRAC 4 cut(s) 256, 262, 450, 489
HindII GTYRAC 4 cut(s) 256, 262, 450, 489
HinfI GANTC 2 cut(s) 227, 576
HpaII CCGG 9 cut(s) 428, 493, 540, 566, 602, 675, 781, 821, 953
HphI GGTGA 6 cut(s) 79, 307, 472, 562, 818, 935
Hpy166II GTNNAC 7 cut(s) 256, 262, 450, 489, 631, 795, 1155
Hpy188I TCNGA 4 cut(s) 148, 214, 226, 355
Hpy188III TCNNGA 3 cut(s) 854, 1202, 1220
Hpy8I GTNNAC 7 cut(s) 256, 262, 450, 489, 631, 795, 1155
Hpy99I CGWCG 9 cut(s) 83, 112, 134, 137, 254, 257, 260, 263, 596
HpyAV CCTTC 4 cut(s) 719, 821, 902, 914
HpyCH4III ACNGT 1 cut(s) 1289
HpyCH4IV ACGT 3 cut(s) 252, 258, 1237
HpyCH4V TGCA 1 cut(s) 877
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 100, 301, 326, 392, 1028
HpySE526I ACGT 3 cut(s) 252, 258, 1237
Hsp92I GRCGYC 2 cut(s) 252, 258
Hsp92II CATG 4 cut(s) 28, 157, 1258, 1277
HspAI GCGC 3 cut(s) 414, 431, 672
KspI CCGCGG 1 cut(s) 330
Kzo9I GATC 1 cut(s) 1114
LmnI GCTCC 3 cut(s) 118, 148, 823
Lsp1109I GCAGC 2 cut(s) 313, 1129
LweI GCATC 2 cut(s) 292, 1030
MaeII ACGT 3 cut(s) 252, 258, 1237
MaeIII GTNAC 5 cut(s) 478, 559, 568, 806, 1130
MalI GATC 1 cut(s) 1116
MbiI CCGCTC 1 cut(s) 181
MboI GATC 1 cut(s) 1114
MfeI CAATTG 1 cut(s) 371
MflI RGATCY 1 cut(s) 1114
MhlI GDGCHC 6 cut(s) 96, 105, 123, 141, 153, 1248
MlsI TGGCCA 1 cut(s) 774
MluCI AATT 8 cut(s) 19, 142, 371, 744, 946, 1174, 1211, 1321
MluNI TGGCCA 1 cut(s) 774
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 805
Mox20I TGGCCA 1 cut(s) 774
MroXI GAANNNNTTC 2 cut(s) 842, 1215
MscI TGGCCA 1 cut(s) 774
MseI TTAA 2 cut(s) 159, 1110
Msp20I TGGCCA 1 cut(s) 774
MspA1I CMGCKG 1 cut(s) 329
MspI CCGG 9 cut(s) 428, 493, 540, 566, 602, 675, 781, 821, 953
MspR9I CCNGG 9 cut(s) 365, 428, 493, 540, 676, 712, 781, 1059, 1249
MunI CAATTG 1 cut(s) 371
MvaI CCWGG 4 cut(s) 365, 712, 1059, 1249
MvnI CGCG 2 cut(s) 107, 329
MwoI GCNNNNNNNGC 6 cut(s) 80, 100, 301, 326, 392, 1028
NciI CCSGG 5 cut(s) 428, 493, 540, 676, 781
NcoI CCATGG 1 cut(s) 1254
NdeII GATC 1 cut(s) 1114
NlaIII CATG 4 cut(s) 28, 157, 1258, 1277
NlaIV GGNNCC 4 cut(s) 120, 150, 357, 819
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 4 cut(s) 478, 559, 568, 806
PcsI WCGNNNNNNNCGW 5 cut(s) 87, 249, 255, 258, 264
PdmI GAANNNNTTC 2 cut(s) 842, 1215
PfeI GAWTC 1 cut(s) 576
PkrI GCNGC 6 cut(s) 73, 76, 303, 328, 331, 1144
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 96
Psp6I CCWGG 4 cut(s) 363, 710, 1057, 1247
PspEI GGTNACC 3 cut(s) 478, 568, 806
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 4 cut(s) 363, 710, 1057, 1247
PspN4I GGNNCC 4 cut(s) 120, 150, 357, 819
PspPI GGNCC 7 cut(s) 355, 367, 661, 705, 792, 1199, 1251
PstNI CAGNNNCTG 1 cut(s) 1025
PsuI RGATCY 1 cut(s) 1114
RsaI GTAC 2 cut(s) 682, 737
RsaNI GTAC 2 cut(s) 681, 736
SacI GAGCTC 1 cut(s) 96
SacII CCGCGG 1 cut(s) 330
SalI GTCGAC 4 cut(s) 254, 260, 448, 487
SaqAI TTAA 2 cut(s) 159, 1110
SatI GCNGC 6 cut(s) 72, 75, 302, 327, 330, 1143
Sau3AI GATC 1 cut(s) 1114
Sau96I GGNCC 7 cut(s) 355, 367, 661, 705, 792, 1199, 1251
ScaI AGTACT 1 cut(s) 682
SchI GAGTC 1 cut(s) 236
ScrFI CCNGG 9 cut(s) 365, 428, 493, 540, 676, 712, 781, 1059, 1249
SduI GDGCHC 6 cut(s) 96, 105, 123, 141, 153, 1248
SfaNI GCATC 2 cut(s) 292, 1030
SfcI CTRYAG 1 cut(s) 1017
Sfr303I CCGCGG 1 cut(s) 330
SgrBI CCGCGG 1 cut(s) 330
SgrDI CGTCGACG 1 cut(s) 254
SinI GGWCC 4 cut(s) 355, 661, 792, 1199
Sse9I AATT 8 cut(s) 19, 142, 371, 744, 946, 1174, 1211, 1321
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 9 cut(s) 363, 426, 491, 538, 674, 710, 779, 1057, 1247
StyI CCWWGG 4 cut(s) 624, 1032, 1096, 1254
TaaI ACNGT 1 cut(s) 1289
TaiI ACGT 3 cut(s) 255, 261, 1240
TaqI TCGA 6 cut(s) 243, 255, 261, 267, 449, 488
TaqII GACCGA 2 cut(s) 218, 678
TasI AATT 8 cut(s) 19, 142, 371, 744, 946, 1174, 1211, 1321
TatI WGTACW 1 cut(s) 680
TauI GCSGC 4 cut(s) 74, 77, 329, 332
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 2 cut(s) 159, 1110
Tru9I TTAA 2 cut(s) 159, 1110
TscAI CASTG 2 cut(s) 802, 1248
TseFI GTSAC 4 cut(s) 478, 559, 568, 806
TseI GCWGC 2 cut(s) 301, 1142
Tsp45I GTSAC 4 cut(s) 478, 559, 568, 806
TspDTI ATGAA 1 cut(s) 1248
TspGWI ACGGA 2 cut(s) 131, 220
TspRI CASTG 2 cut(s) 802, 1248
VpaK11BI GGWCC 4 cut(s) 355, 661, 792, 1199
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 2 cut(s) 946, 1211
XcmI CCANNNNNNNNNTGG 2 cut(s) 718, 782
XmiI GTMKAC 6 cut(s) 255, 261, 449, 488, 630, 1154
XmnI GAANNNNTTC 2 cut(s) 842, 1215
ZraI GACGTC 2 cut(s) 253, 259
ZrmI AGTACT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.