RLG00000035210

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018914
Physical Location & Seq
Reverse (-)
62889903 .. 62891781
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035210

Sequence Viewer

Length: 1008 bp
ATGAGTTTGATGGGACATGCTGGGAAGCGAAGAGCCTTTGGCAGAGTCAGAGTCGCAACTGAGGGCTCAACTTCAGTTGACACTTTGGCTGATGACTATTACTCTGTCTTGGGTTTGCTTCCAGATGCAACACCAGCACAGATCAAGAAAGCCTATTACAATTGTATGAAGGCTTGTCATCCTGACTTGAGTGGTGATAATCCGGAGACAACAAATTGCTGTATGTTCATCAATGAGGTCTATGAGCCGTTCCGTGACCATCGGAGTTGGCCGCAGTATGAATCATGTGATGGCTCTTTTCCAGTTGTTCTGCATGCCAGAAGAAGTAAAATAGAACTCAGGTTAATTCAGTACTGTATAAGTGCATGGAAATCAATCTCCTTCCTCAGCTCAAATCTTCCCCAATTAAGAATGGAAACTACTTTAGTACTGTTGATTTCTCTTTTCCTCATTTTCAATGCCGTCGCCTCAACTCTCTCTTGTGATCCCTCAGGCTCTATACTTTGCCAGGGAACATCCTTCCCTACATTCACATGCTCGCCGCCGGTAACGTCACCAACTGGAGCCAAATTGACCCTCAACGATTTCAGCAAATGCGGTGACGGCGGGCACCCATCGGAGTGTGACGGGCAATATCATGACAACAGTGAGCTAATTGTGGCACTATCCACTGGTTGGTACAATAGTGGGTCCCGTTGTGGGAGCATGATAACAGTTGCGGCAACTAATGGGGCGGGTGTGATGGCTAAAGTGGCGGACGAATGTGATTCGCGTGCTGGATGTAACGGAGAACATGGCCGTCAGCCGCCGTGCTTGAGCAATATTATTGACGGCTCTGCAGCTGTGTGGAATGCTTTGGGACTTGATCGGGGTAGGAATTGTGGACGTTGCTTGGGCCATGACCTAGCTAGTTACAATGCAAGTACATTACCTGTACTAATGCTAATTAAAAATAAAAAAGACCACCATACAACTCCAAGAAGTACTAGCTACCTCTTAGGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

336

Amino Acids

36.2

Weight (kDa)

6.77

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 32 - 93 1.9e-12 DnaJ domain
KWL1 PF24300 164 - 290 9.7e-43 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018914)

Species Orthologous Gene IDs
prunus_persica Prupe.6G105500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0058291 RchiOBHm_Chr5g0058321
rosa_laevigata RLG00000035210 RLG00000035211
rosa_roxburghii Rroxscaffold_1G00022240 Rroxscaffold_1G00022260
rosa_rugosa Rorug05G0313000
rosa_samantha Rh5BG392100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 609
AccB7I CCANNNNNTGG 1 cut(s) 675
AccII CGCG 1 cut(s) 772
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 8 cut(s) 272, 542, 597, 606, 719, 734, 755, 806
AclWI GGATC 1 cut(s) 479
AcoI YGGCCR 2 cut(s) 269, 796
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 6 cut(s) 353, 429, 680, 925, 936, 985
AfiI CCNNNNNNNGG 2 cut(s) 675, 699
AgsI TTSAA 1 cut(s) 457
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 5 cut(s) 390, 652, 842, 908, 990
AluI AGCT 5 cut(s) 390, 652, 842, 908, 990
Alw26I GTCTC 1 cut(s) 200
AlwI GGATC 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 3 cut(s) 269, 796, 895
ApeKI GCWGC 1 cut(s) 839
AspS9I GGNCC 2 cut(s) 690, 895
AsuHPI GGTGA 3 cut(s) 206, 546, 611
AvaII GGWCC 1 cut(s) 690
AxyI CCTNAGG 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 612
BaeI ACNNNNGTAYC 2 cut(s) 670, 703
BanI GGYRCC 1 cut(s) 609
BanII GRGCYC 1 cut(s) 68
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 1 cut(s) 851
BccI CCATC 5 cut(s) 4, 267, 284, 622, 736
BceAI ACGGC 6 cut(s) 232, 446, 619, 783, 793, 847
BciT130I CCWGG 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 200
BfaI CTAG 3 cut(s) 905, 909, 987
BfmI CTRYAG 1 cut(s) 837
BisI GCNGC 5 cut(s) 272, 542, 720, 806, 840
BlsI GCNGC 5 cut(s) 273, 543, 721, 807, 841
BmcAI AGTACT 3 cut(s) 353, 429, 985
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 690
BmgT120I GGNCC 2 cut(s) 690, 895
BmiI GGNNCC 4 cut(s) 565, 611, 691, 692
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 1 cut(s) 115
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 386
BpuEI CTTGAG 2 cut(s) 208, 835
BsaJI CCNNGG 1 cut(s) 508
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 675, 699
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 3 cut(s) 302, 565, 676
Bse21I CCTNAGG 1 cut(s) 490
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 3 cut(s) 178, 515, 785
BseLI CCNNNNNNNGG 2 cut(s) 675, 699
BseMII CTCAG 4 cut(s) 51, 352, 400, 504
BseNI ACTGG 3 cut(s) 302, 565, 676
BseSI GKGCMC 1 cut(s) 612
BseXI GCAGC 1 cut(s) 851
BseYI CCCAGC 1 cut(s) 20
Bsh1236I CGCG 1 cut(s) 772
BshFI GGCC 3 cut(s) 271, 798, 897
BshNI GGYRCC 1 cut(s) 609
BsiSI CCGG 2 cut(s) 203, 545
BslFI GGGAC 3 cut(s) 27, 676, 873
BslI CCNNNNNNNGG 2 cut(s) 675, 699
BsmAI GTCTC 1 cut(s) 200
BsmFI GGGAC 3 cut(s) 27, 676, 873
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 3 cut(s) 271, 798, 897
Bsp1286I GDGCHC 2 cut(s) 68, 612
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 4 cut(s) 141, 484, 865, 1001
BspACI CCGC 8 cut(s) 272, 542, 597, 606, 719, 734, 755, 806
BspANI GGCC 3 cut(s) 271, 798, 897
BspCNI CTCAG 4 cut(s) 52, 351, 399, 503
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 772
BspHI TCATGA 2 cut(s) 637, 1004
BspLI GGNNCC 4 cut(s) 565, 611, 691, 692
BspMAI CTGCAG 1 cut(s) 841
BspPI GGATC 1 cut(s) 479
BspQI GCTCTTC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 609
BsrFI RCCGGY 1 cut(s) 544
BsrI ACTGG 3 cut(s) 302, 565, 676
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 4 cut(s) 141, 484, 865, 1001
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 4 cut(s) 356, 432, 647, 715
Bst6I CTCTTC 1 cut(s) 25
BstC8I GCNNGC 4 cut(s) 315, 539, 608, 774
BstDEI CTNAG 5 cut(s) 60, 338, 386, 490, 997
BstF5I GGATG 3 cut(s) 178, 515, 785
BstFNI CGCG 1 cut(s) 772
BstKTI GATC 4 cut(s) 144, 487, 868, 1004
BstMAI GTCTC 1 cut(s) 200
BstMBI GATC 4 cut(s) 141, 484, 865, 1001
BstMWI GCNNNNNNNGC 3 cut(s) 134, 603, 752
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 3 cut(s) 20, 317, 537
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 837
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 1 cut(s) 772
BstV1I GCAGC 1 cut(s) 851
Bsu36I CCTNAGG 1 cut(s) 490
BsuRI GGCC 3 cut(s) 271, 798, 897
BtsCI GGATG 3 cut(s) 178, 515, 785
BtsIMutI CAGTG 2 cut(s) 652, 669
Cac8I GCNNGC 4 cut(s) 315, 539, 608, 774
CciI TCATGA 2 cut(s) 637, 1004
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 2 cut(s) 690, 895
Csp6I GTAC 6 cut(s) 352, 428, 679, 924, 935, 984
CviQI GTAC 6 cut(s) 352, 428, 679, 924, 935, 984
DdeI CTNAG 5 cut(s) 60, 338, 386, 490, 997
DpnI GATC 4 cut(s) 143, 486, 867, 1003
DpnII GATC 4 cut(s) 141, 484, 865, 1001
EaeI YGGCCR 2 cut(s) 269, 796
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 770
Eco24I GRGCYC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 490
EcoO109I RGGNCCY 1 cut(s) 690
EcoRII CCWGG 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 68
FaqI GGGAC 3 cut(s) 27, 676, 873
FauI CCCGC 2 cut(s) 599, 727
Fnu4HI GCNGC 5 cut(s) 272, 542, 720, 806, 840
FokI GGATG 3 cut(s) 165, 502, 792
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 5 cut(s) 272, 542, 720, 806, 840
FspBI CTAG 3 cut(s) 905, 909, 987
GluI GCNGC 5 cut(s) 272, 542, 720, 806, 840
GsaI CCCAGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 3 cut(s) 271, 798, 897
HapII CCGG 2 cut(s) 203, 545
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 4 cut(s) 45, 51, 281, 767
HpaII CCGG 2 cut(s) 203, 545
HphI GGTGA 3 cut(s) 206, 546, 611
Hpy166II GTNNAC 2 cut(s) 79, 884
Hpy188I TCNGA 3 cut(s) 50, 264, 619
Hpy188III TCNNGA 6 cut(s) 122, 145, 182, 203, 638, 1005
Hpy8I GTNNAC 2 cut(s) 79, 884
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 3 cut(s) 163, 391, 529
HpyCH4III ACNGT 4 cut(s) 356, 432, 647, 715
HpyCH4IV ACGT 2 cut(s) 551, 886
HpyCH4V TGCA 5 cut(s) 128, 313, 365, 839, 920
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 603, 752
HpyF3I CTNAG 5 cut(s) 60, 338, 386, 490, 997
HpySE526I ACGT 2 cut(s) 551, 886
KflI GGGWCCC 1 cut(s) 690
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 4 cut(s) 141, 484, 865, 1001
LguI GCTCTTC 1 cut(s) 25
LmnI GCTCC 2 cut(s) 563, 702
Lsp1109I GCAGC 1 cut(s) 851
LweI GCATC 1 cut(s) 115
MaeI CTAG 3 cut(s) 905, 909, 987
MaeII ACGT 2 cut(s) 551, 886
MaeIII GTNAC 7 cut(s) 254, 547, 552, 599, 623, 782, 911
MalI GATC 4 cut(s) 143, 486, 867, 1003
MboI GATC 4 cut(s) 141, 484, 865, 1001
MboII GAAGA 3 cut(s) 42, 333, 389
MfeI CAATTG 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 68, 612
MluCI AATT 8 cut(s) 160, 214, 345, 404, 569, 654, 877, 945
MlyI GAGTC 2 cut(s) 54, 60
MnlI CCTC 8 cut(s) 55, 229, 395, 458, 478, 499, 587, 1004
MroI TCCGGA 1 cut(s) 202
MseI TTAA 3 cut(s) 344, 407, 948
MslI CAYNNNNRTG 2 cut(s) 532, 619
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 2 cut(s) 203, 545
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 1 cut(s) 160
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 1 cut(s) 509
MvnI CGCG 1 cut(s) 772
MwoI GCNNNNNNNGC 3 cut(s) 134, 603, 752
NdeII GATC 4 cut(s) 141, 484, 865, 1001
NlaIV GGNNCC 4 cut(s) 565, 611, 691, 692
NmuCI GTSAC 4 cut(s) 254, 552, 599, 623
NspI RCATGY 3 cut(s) 20, 317, 537
PaeI GCATGC 1 cut(s) 317
PagI TCATGA 2 cut(s) 637, 1004
PciSI GCTCTTC 1 cut(s) 25
PctI GAATGC 1 cut(s) 856
PfeI GAWTC 2 cut(s) 281, 767
PflMI CCANNNNNTGG 1 cut(s) 675
PkrI GCNGC 5 cut(s) 273, 543, 721, 807, 841
PleI GAGTC 2 cut(s) 53, 59
PpsI GAGTC 2 cut(s) 53, 59
PpuMI RGGWCCY 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 20
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 4 cut(s) 565, 611, 691, 692
PspPI GGNCC 2 cut(s) 690, 895
PspPPI RGGWCCY 1 cut(s) 690
PstI CTGCAG 1 cut(s) 841
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 6 cut(s) 353, 429, 680, 925, 936, 985
RsaNI GTAC 6 cut(s) 352, 428, 679, 924, 935, 984
RseI CAYNNNNRTG 2 cut(s) 532, 619
SapI GCTCTTC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 344, 407, 948
SatI GCNGC 5 cut(s) 272, 542, 720, 806, 840
Sau3AI GATC 4 cut(s) 141, 484, 865, 1001
Sau96I GGNCC 2 cut(s) 690, 895
ScaI AGTACT 3 cut(s) 353, 429, 985
SchI GAGTC 2 cut(s) 54, 60
ScrFI CCNGG 1 cut(s) 509
SduI GDGCHC 2 cut(s) 68, 612
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 837
SinI GGWCC 1 cut(s) 690
SmiMI CAYNNNNRTG 2 cut(s) 532, 619
SmlI CTYRAG 2 cut(s) 187, 814
SmoI CTYRAG 2 cut(s) 187, 814
SphI GCATGC 1 cut(s) 317
Sse9I AATT 8 cut(s) 160, 214, 345, 404, 569, 654, 877, 945
SsiI CCGC 8 cut(s) 272, 542, 597, 606, 719, 734, 755, 806
SspI AATATT 1 cut(s) 823
SspMI CTAG 3 cut(s) 905, 909, 987
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 4 cut(s) 356, 432, 647, 715
TaiI ACGT 2 cut(s) 554, 889
TasI AATT 8 cut(s) 160, 214, 345, 404, 569, 654, 877, 945
TatI WGTACW 5 cut(s) 351, 427, 923, 934, 983
TauI GCSGC 4 cut(s) 274, 544, 722, 808
TfiI GAWTC 2 cut(s) 281, 767
Tru1I TTAA 3 cut(s) 344, 407, 948
Tru9I TTAA 3 cut(s) 344, 407, 948
TscAI CASTG 2 cut(s) 652, 676
TseFI GTSAC 4 cut(s) 254, 552, 599, 623
TseI GCWGC 1 cut(s) 839
Tsp45I GTSAC 4 cut(s) 254, 552, 599, 623
TspDTI ATGAA 3 cut(s) 182, 217, 294
TspGWI ACGGA 2 cut(s) 242, 801
TspRI CASTG 2 cut(s) 652, 676
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 690
XceI RCATGY 3 cut(s) 20, 317, 537
XspI CTAG 3 cut(s) 905, 909, 987
ZrmI AGTACT 3 cut(s) 353, 429, 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.