Rroxscaffold_1G00022260

Kiwellin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018914
Physical Location & Seq
Forward (+)
27507458 .. 27507934
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00022260.1

Sequence Viewer

Length: 477 bp
ATGGAAAGTACTTTAGTACTGTCGATTTCTCTTTTCCTCATTTTCAATGCCGTCGCCTCAACTCTCTCTTGTGATCCCTCAGGCTCTATAGTTTGCCAGGGAGCATTCTTCCCTACATTCACATGCTCGCCGCCAGTAACGTCACCAACTGGACCCAAACGGACCCTCAACGATTTCAGCAAAGGCTGTGACGGCGGGCAATATCATGACAACAGTGAGCTAATTCTGGCACTATCCACCGGGTGGTACAATAGTACGTCCCGTTGTGGGAGCATGATAACAGTTGCGGCAACTAATGGGGCGGTTGTGATGGCTAAAGTGGCGGACGAATGTGATTCGCGTGCTGGATGTAACGGAGAACATGGCCGTCAGCCGCCGTGCTTGAGCACTATTATTGACGGCTCTGCAGCTGTGTGGAATGCTTTGGGACTTGATCAATGGGTACGAATTGTGAACGTTGGTTGGGCCATGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

16.59

Weight (kDa)

4.88

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 27 - 155 1.4e-35 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018914)

Species Orthologous Gene IDs
prunus_persica Prupe.6G105500_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0058291 RchiOBHm_Chr5g0058321
rosa_laevigata RLG00000035210 RLG00000035211
rosa_roxburghii Rroxscaffold_1G00022240 Rroxscaffold_1G00022260
rosa_rugosa Rorug05G0313000
rosa_samantha Rh5BG392100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 243
AccII CGCG 1 cut(s) 340
AciI CCGC 6 cut(s) 131, 195, 287, 302, 323, 374
AclI AACGTT 1 cut(s) 456
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 364
AdeI CACNNNGTG 1 cut(s) 243
AfaI GTAC 5 cut(s) 10, 18, 248, 256, 444
AfiI CCNNNNNNNGG 2 cut(s) 243, 267
AgsI TTSAA 1 cut(s) 46
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 2 cut(s) 220, 410
AluI AGCT 2 cut(s) 220, 410
Alw21I GWGCWC 1 cut(s) 389
AlwI GGATC 1 cut(s) 68
AoxI GGCC 2 cut(s) 364, 465
ApeKI GCWGC 1 cut(s) 407
AspS9I GGNCC 3 cut(s) 152, 162, 465
AsuC2I CCSGG 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 135
AvaII GGWCC 2 cut(s) 152, 162
AxyI CCTNAGG 1 cut(s) 79
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
Bbv12I GWGCWC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 419
BccI CCATC 1 cut(s) 304
BceAI ACGGC 5 cut(s) 35, 208, 351, 361, 415
BciT130I CCWGG 1 cut(s) 98
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 241
BfaI CTAG 1 cut(s) 475
BfmI CTRYAG 2 cut(s) 87, 405
BisI GCNGC 4 cut(s) 131, 288, 374, 408
BlsI GCNGC 4 cut(s) 132, 289, 375, 409
BmcAI AGTACT 2 cut(s) 10, 18
Bme1390I CCNGG 2 cut(s) 98, 241
Bme18I GGWCC 2 cut(s) 152, 162
BmgT120I GGNCC 3 cut(s) 152, 162, 465
BmiI GGNNCC 2 cut(s) 154, 164
BmrFI CCNGG 2 cut(s) 98, 241
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 1 cut(s) 241
BsaJI CCNNGG 1 cut(s) 97
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 267
Bse1I ACTGG 2 cut(s) 134, 154
Bse21I CCTNAGG 1 cut(s) 79
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 243, 267
BseMII CTCAG 1 cut(s) 93
BseNI ACTGG 2 cut(s) 134, 154
BseXI GCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 340
BshFI GGCC 2 cut(s) 366, 467
BsiHKAI GWGCWC 1 cut(s) 389
BsiSI CCGG 1 cut(s) 240
BslFI GGGAC 2 cut(s) 244, 441
BslI CCNNNNNNNGG 2 cut(s) 243, 267
BsmFI GGGAC 2 cut(s) 244, 441
BsmI GAATGC 2 cut(s) 104, 424
BsnI GGCC 2 cut(s) 366, 467
Bsp1286I GDGCHC 1 cut(s) 389
Bsp143I GATC 2 cut(s) 73, 433
BspACI CCGC 6 cut(s) 131, 195, 287, 302, 323, 374
BspANI GGCC 2 cut(s) 366, 467
BspCNI CTCAG 1 cut(s) 92
BspFNI CGCG 1 cut(s) 340
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 154, 164
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 1 cut(s) 68
BsrI ACTGG 2 cut(s) 134, 154
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 2 cut(s) 73, 433
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 21, 215, 283
BstC8I GCNNGC 3 cut(s) 128, 197, 342
BstDEI CTNAG 1 cut(s) 79
BstF5I GGATG 1 cut(s) 353
BstFNI CGCG 1 cut(s) 340
BstKTI GATC 2 cut(s) 76, 436
BstMBI GATC 2 cut(s) 73, 433
BstMWI GCNNNNNNNGC 2 cut(s) 192, 320
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 2 cut(s) 96, 239
BstSFI CTRYAG 2 cut(s) 87, 405
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 1 cut(s) 419
Bsu36I CCTNAGG 1 cut(s) 79
BsuRI GGCC 2 cut(s) 366, 467
BtsCI GGATG 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 3 cut(s) 128, 197, 342
CciI TCATGA 1 cut(s) 205
Cfr13I GGNCC 3 cut(s) 152, 162, 465
Csp6I GTAC 5 cut(s) 9, 17, 247, 255, 443
CviAII CATG 5 cut(s) 123, 206, 274, 362, 469
CviJI RGCY 9 cut(s) 84, 186, 220, 314, 366, 373, 402, 410, 467
CviKI_1 RGCY 9 cut(s) 84, 186, 220, 314, 366, 373, 402, 410, 467
CviQI GTAC 5 cut(s) 9, 17, 247, 255, 443
DdeI CTNAG 1 cut(s) 79
DpnI GATC 2 cut(s) 75, 435
DpnII GATC 2 cut(s) 73, 433
DraIII CACNNNGTG 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 364
EciI GGCGGA 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 152, 162
Eco81I CCTNAGG 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 96
FaeI CATG 5 cut(s) 126, 209, 277, 365, 472
FaiI YATR 6 cut(s) 89, 124, 207, 275, 363, 470
FaqI GGGAC 2 cut(s) 244, 441
FatI CATG 5 cut(s) 122, 205, 273, 361, 468
FauI CCCGC 1 cut(s) 188
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 131, 288, 374, 408
FokI GGATG 1 cut(s) 360
Fsp4HI GCNGC 4 cut(s) 131, 288, 374, 408
FspBI CTAG 1 cut(s) 475
GluI GCNGC 4 cut(s) 131, 288, 374, 408
HaeIII GGCC 2 cut(s) 366, 467
HapII CCGG 1 cut(s) 240
Hin1II CATG 5 cut(s) 126, 209, 277, 365, 472
HinfI GANTC 1 cut(s) 335
HpaII CCGG 1 cut(s) 240
HphI GGTGA 1 cut(s) 135
Hpy166II GTNNAC 1 cut(s) 454
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 454
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 3 cut(s) 21, 215, 283
HpyCH4IV ACGT 3 cut(s) 140, 257, 456
HpyCH4V TGCA 1 cut(s) 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 192, 320
HpyF3I CTNAG 1 cut(s) 79
HpySE526I ACGT 3 cut(s) 140, 257, 456
Hsp92II CATG 5 cut(s) 126, 209, 277, 365, 472
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 2 cut(s) 73, 433
LmnI GCTCC 2 cut(s) 101, 270
LpnPI CCDG 8 cut(s) 66, 83, 110, 135, 147, 212, 253, 330
Lsp1109I GCAGC 1 cut(s) 419
MaeI CTAG 1 cut(s) 475
MaeII ACGT 3 cut(s) 140, 257, 456
MaeIII GTNAC 4 cut(s) 136, 141, 188, 350
MalI GATC 2 cut(s) 75, 435
MboI GATC 2 cut(s) 73, 433
MboII GAAGA 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 389
MluCI AATT 2 cut(s) 222, 447
MnlI CCTC 4 cut(s) 47, 67, 88, 176
MslI CAYNNNNRTG 1 cut(s) 121
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 240
MspR9I CCNGG 2 cut(s) 98, 241
Mva1269I GAATGC 2 cut(s) 104, 424
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 2 cut(s) 192, 320
NciI CCSGG 1 cut(s) 241
NdeII GATC 2 cut(s) 73, 433
NlaIII CATG 5 cut(s) 126, 209, 277, 365, 472
NlaIV GGNNCC 2 cut(s) 154, 164
NmuCI GTSAC 2 cut(s) 141, 188
NspI RCATGY 1 cut(s) 126
PagI TCATGA 1 cut(s) 205
PctI GAATGC 2 cut(s) 104, 424
PfeI GAWTC 1 cut(s) 335
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 4 cut(s) 132, 289, 375, 409
Psp1406I AACGTT 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 154, 164
PspPI GGNCC 3 cut(s) 152, 162, 465
PstI CTGCAG 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 5 cut(s) 10, 18, 248, 256, 444
RsaNI GTAC 5 cut(s) 9, 17, 247, 255, 443
RseI CAYNNNNRTG 1 cut(s) 121
SatI GCNGC 4 cut(s) 131, 288, 374, 408
Sau3AI GATC 2 cut(s) 73, 433
Sau96I GGNCC 3 cut(s) 152, 162, 465
ScaI AGTACT 2 cut(s) 10, 18
ScrFI CCNGG 2 cut(s) 98, 241
SduI GDGCHC 1 cut(s) 389
SetI ASST 6 cut(s) 143, 222, 260, 412, 459, 476
SfcI CTRYAG 2 cut(s) 87, 405
SinI GGWCC 2 cut(s) 152, 162
SmiMI CAYNNNNRTG 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 2 cut(s) 222, 447
SsiI CCGC 6 cut(s) 131, 195, 287, 302, 323, 374
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 2 cut(s) 96, 239
TaaI ACNGT 3 cut(s) 21, 215, 283
TaiI ACGT 3 cut(s) 143, 260, 459
TaqI TCGA 1 cut(s) 23
TasI AATT 2 cut(s) 222, 447
TatI WGTACW 2 cut(s) 8, 16
TauI GCSGC 3 cut(s) 133, 290, 376
TfiI GAWTC 1 cut(s) 335
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 2 cut(s) 141, 188
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 2 cut(s) 141, 188
TspGWI ACGGA 2 cut(s) 175, 369
TspRI CASTG 1 cut(s) 220
Van91I CCANNNNNTGG 1 cut(s) 243
VpaK11BI GGWCC 2 cut(s) 152, 162
XceI RCATGY 1 cut(s) 126
XspI CTAG 1 cut(s) 475
ZrmI AGTACT 2 cut(s) 10, 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.