Rmu_co8323009.1_g000001

serine threonine-protein kinase HT1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8323009.1
OG0005049
Physical Location & Seq
Forward (+)
1 .. 953
953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8323009.1_g000001.1.cds

Sequence Viewer

Length: 604 bp
cttgcagaaagcctcctgtttattgtgtagtcacagaatatttagatgagggttccttgagggcttacttgcacaaacttgagtgcaaatctcttcctttagagaaactaattgccattgccttggacattgctcggggaatggaatacatccactcgcaaggagtcattcaccgggaccttaagcctgagaatgttcttattgataaagactttaggctgaaaattgctgattttggcatagcatgtgaggaggcatactgtgattcattggctgatgaccctggaacttaccgttggatggcccccgaaatgattaagcgtaaatcctatgggcgaaaggttgatgtctacagttttggactcgttttgtgggaactagtagccggaacaatcccttatgaggagatgaatcccattcaagcggcttttgcagttgtgaataagaatttgaggccggttatcccaaagaactgtccacctgctatgcgagccttgatcgagcaatgctggtcgttgcagccggataaaagacccgagttttggcaggttgtaaaggtgctagaacaatttgagtcttcccttgcccgagatggaacattgac
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

201

Amino Acids

22.99

Weight (kDa)

6.0

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 489
Acc36I ACCTGC 2 cut(s) 489, 537
AccI GTMKAC 1 cut(s) 350
AciI CCGC 1 cut(s) 424
AcsI RAATTY 1 cut(s) 447
AfiI CCNNNNNNNGG 3 cut(s) 300, 402, 542
AflII CTTAAG 1 cut(s) 181
AgsI TTSAA 1 cut(s) 421
AhlI ACTAGT 1 cut(s) 378
AjnI CCWGG 1 cut(s) 282
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
Ama87I CYCGRG 3 cut(s) 134, 535, 587
AoxI GGCC 2 cut(s) 302, 454
ApeKI GCWGC 1 cut(s) 519
ApoI RAATTY 1 cut(s) 447
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
AspS9I GGNCC 2 cut(s) 177, 303
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 163
AvaI CYCGRG 3 cut(s) 134, 535, 587
AvaII GGWCC 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 569
BbvI GCAGC 1 cut(s) 531
BccI CCATC 2 cut(s) 294, 586
BciT130I CCWGG 1 cut(s) 284
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 378
BfaI CTAG 2 cut(s) 379, 562
BfmI CTRYAG 1 cut(s) 351
BfrI CTTAAG 1 cut(s) 181
BfuAI ACCTGC 2 cut(s) 489, 537
BisI GCNGC 2 cut(s) 425, 520
BlsI GCNGC 2 cut(s) 426, 521
Bme1390I CCNGG 2 cut(s) 175, 284
Bme18I GGWCC 1 cut(s) 177
BmeT110I CYCGRG 3 cut(s) 134, 535, 587
BmgT120I GGNCC 2 cut(s) 177, 303
BmiI GGNNCC 3 cut(s) 54, 178, 305
BmrFI CCNGG 2 cut(s) 175, 284
BpiI GAAGAC 1 cut(s) 569
BpuEI CTTGAG 2 cut(s) 78, 100
BpuMI CCSGG 1 cut(s) 175
BsaJI CCNNGG 2 cut(s) 122, 282
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 402, 542
Bse118I RCCGGY 1 cut(s) 456
Bse3DI GCAATG 3 cut(s) 116, 128, 511
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 2 cut(s) 122, 282
BseGI GGATG 2 cut(s) 149, 305
BseLI CCNNNNNNNGG 3 cut(s) 300, 402, 542
BseMI GCAATG 3 cut(s) 116, 128, 511
BseMII CTCAG 1 cut(s) 179
BseRI GAGGAG 2 cut(s) 265, 418
BseXI GCAGC 1 cut(s) 531
BshFI GGCC 2 cut(s) 304, 456
BsiHKCI CYCGRG 3 cut(s) 134, 535, 587
BsiSI CCGG 4 cut(s) 174, 386, 457, 523
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 3 cut(s) 300, 402, 542
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 2 cut(s) 304, 456
BsoBI CYCGRG 3 cut(s) 134, 535, 587
Bsp143I GATC 1 cut(s) 497
BspACI CCGC 1 cut(s) 424
BspANI GGCC 2 cut(s) 304, 456
BspCNI CTCAG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 54, 178, 305
BspMI ACCTGC 2 cut(s) 489, 537
BspTI CTTAAG 1 cut(s) 181
BsrDI GCAATG 3 cut(s) 116, 128, 511
BsrFI RCCGGY 1 cut(s) 456
BssAI RCCGGY 1 cut(s) 456
BssECI CCNNGG 2 cut(s) 122, 282
BssMI GATC 1 cut(s) 497
BssT1I CCWWGG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 4 cut(s) 262, 295, 355, 475
Bst6I CTCTTC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 491
BstDEI CTNAG 1 cut(s) 188
BstF5I GGATG 2 cut(s) 149, 305
BstKTI GATC 1 cut(s) 500
BstMBI GATC 1 cut(s) 497
BstMWI GCNNNNNNNGC 2 cut(s) 430, 490
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 173, 282
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 531
BstV2I GAAGAC 1 cut(s) 569
BstXI CCANNNNNNTGG 1 cut(s) 123
BsuRI GGCC 2 cut(s) 304, 456
BtsCI GGATG 2 cut(s) 149, 305
BveI ACCTGC 2 cut(s) 489, 537
Cac8I GCNNGC 1 cut(s) 491
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 2 cut(s) 177, 303
CviAII CATG 1 cut(s) 245
DdeI CTNAG 1 cut(s) 188
DpnI GATC 1 cut(s) 499
DpnII GATC 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco130I CCWWGG 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 177
Eco88I CYCGRG 3 cut(s) 134, 535, 587
EcoO109I RGGNCCY 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 1 cut(s) 248
FaiI YATR 6 cut(s) 241, 246, 258, 332, 401, 487
FaqI GGGAC 1 cut(s) 190
FatI CATG 1 cut(s) 244
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 2 cut(s) 425, 520
FokI GGATG 2 cut(s) 136, 312
Fsp4HI GCNGC 2 cut(s) 425, 520
FspBI CTAG 2 cut(s) 379, 562
GluI GCNGC 2 cut(s) 425, 520
HaeIII GGCC 2 cut(s) 304, 456
HapII CCGG 4 cut(s) 174, 386, 457, 523
Hin1II CATG 1 cut(s) 248
HinfI GANTC 5 cut(s) 164, 265, 362, 411, 574
HpaII CCGG 4 cut(s) 174, 386, 457, 523
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 2 cut(s) 351, 478
Hpy8I GTNNAC 2 cut(s) 351, 478
HpyCH4III ACNGT 4 cut(s) 262, 295, 355, 475
HpyCH4V TGCA 5 cut(s) 5, 72, 86, 433, 519
HpyF10VI GCNNNNNNNGC 2 cut(s) 430, 490
HpyF3I CTNAG 1 cut(s) 188
Hsp92II CATG 1 cut(s) 248
Kzo9I GATC 1 cut(s) 497
Lsp1109I GCAGC 1 cut(s) 531
MaeI CTAG 2 cut(s) 379, 562
MaeIII GTNAC 1 cut(s) 30
MalI GATC 1 cut(s) 499
MboI GATC 1 cut(s) 497
MboII GAAGA 2 cut(s) 85, 569
MluCI AATT 4 cut(s) 110, 224, 447, 568
MlyI GAGTC 3 cut(s) 173, 356, 583
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 7 cut(s) 23, 42, 53, 243, 246, 396, 446
MseI TTAA 2 cut(s) 182, 317
MspCI CTTAAG 1 cut(s) 181
MspI CCGG 4 cut(s) 174, 386, 457, 523
MspR9I CCNGG 2 cut(s) 175, 284
MvaI CCWGG 1 cut(s) 284
MwoI GCNNNNNNNGC 2 cut(s) 430, 490
NciI CCSGG 1 cut(s) 175
NdeII GATC 1 cut(s) 497
NlaIII CATG 1 cut(s) 248
NlaIV GGNNCC 3 cut(s) 54, 178, 305
NmuCI GTSAC 1 cut(s) 30
NspI RCATGY 1 cut(s) 248
PaqCI CACCTGC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 265, 411
PkrI GCNGC 2 cut(s) 426, 521
PleI GAGTC 3 cut(s) 172, 356, 582
PpsI GAGTC 3 cut(s) 172, 356, 582
PpuMI RGGWCCY 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 282
PspGI CCWGG 1 cut(s) 282
PspN4I GGNNCC 3 cut(s) 54, 178, 305
PspPI GGNCC 2 cut(s) 177, 303
PspPPI RGGWCCY 1 cut(s) 177
SaqAI TTAA 2 cut(s) 182, 317
SatI GCNGC 2 cut(s) 425, 520
Sau3AI GATC 1 cut(s) 497
Sau96I GGNCC 2 cut(s) 177, 303
SchI GAGTC 3 cut(s) 173, 356, 583
ScrFI CCNGG 2 cut(s) 175, 284
SetI ASST 5 cut(s) 182, 344, 483, 551, 560
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 1 cut(s) 177
SmlI CTYRAG 3 cut(s) 57, 79, 181
SmoI CTYRAG 3 cut(s) 57, 79, 181
SpeI ACTAGT 1 cut(s) 378
Sse9I AATT 4 cut(s) 110, 224, 447, 568
SsiI CCGC 1 cut(s) 424
SspI AATATT 1 cut(s) 40
SspMI CTAG 2 cut(s) 379, 562
StyD4I CCNGG 2 cut(s) 173, 282
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 4 cut(s) 262, 295, 355, 475
TaqI TCGA 1 cut(s) 500
TasI AATT 4 cut(s) 110, 224, 447, 568
TauI GCSGC 1 cut(s) 427
TfiI GAWTC 2 cut(s) 265, 411
Tru1I TTAA 2 cut(s) 182, 317
Tru9I TTAA 2 cut(s) 182, 317
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 1 cut(s) 519
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 2 cut(s) 257, 424
Vha464I CTTAAG 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 177
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 350
XspI CTAG 2 cut(s) 379, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.