Rmu_sc0000037.1_g000021

Protein of unknown function (DUF630)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000037.1
OG0005108
Physical Location & Seq
Forward (+)
130749 .. 132147
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
atgaaaatgctgaacccagtagctttatttcgctcagcatcctcgtctcgctcagcctcatccagatttttagtgacttcttcctgctccaaagatgaaggttatgaaagcagcagtgacttctcagaggagccctgcatgttttcaggtagtcaccaatcaacattggacagattgtatgcttgggagaagaaactctatgaggaagtcaagtctggagaaaaggttcggatgacatatgacaagaaattaaagcaactccggaaccaagatgtaaaaggctatgaccaatctgctctagaaaaaacaagggtatccattagagatctgcatacgcagatgaaggtttcgatacactcagttgaagctatttcaaagagaattgaaaccttaagggatgaagaactgcagcctcaactttcagaattggtgcaagggttggcaaggatgtggaaagtaatggcagagtgtcatcagacacagaaacgaagcctcgatgaagccaagcttctactagcaggcacaccttcaaaactgcaagctaaaaggctttcttcttccagttcagttactgatccaaaccagctagcacgttctgcagccaatcttgagaccgaactgaggaattggagagcctatttcgagtcatggatcacttctcagcgatcctacgtacacgcattgacaggatggcttctccgttgcatgagggctgatccagatgcatcaaagttgcctctctctcctcgtcgctcttacagtgctcctccaatatttggaatatgcatccaatggtcaaggtttctagatgccatacgtgagaccccagtgctagatggactagattttttcgcagctggtatgggatctctctatgcacagcaggttaaagaggatttacgtagagctcggataggttcaaagaggttcgagtcaactgaggaattcagtgggaatatggaactggtggaagttggccaagttgaacaagtaatgactgcagataaaatggctgaagttgccataagggtactttgtgctggaatgtcagttactatgagctcactgacagagttttctattgcttctgctgaggggtatgctgatcttgtgaatcaatgggacgatgccaagactagagcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

381

Amino Acids

43.0

Weight (kDa)

8.11

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 9 - 288 7e-95 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 874
AccB7I CCANNNNNTGG 1 cut(s) 776
AccIII TCCGGA 1 cut(s) 261
AclWI GGATC 5 cut(s) 569, 659, 660, 710, 874
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 1 cut(s) 944
AcuI CTGAAG 1 cut(s) 1035
AfaI GTAC 2 cut(s) 675, 1032
AfiI CCNNNNNNNGG 2 cut(s) 621, 776
AflII CTTAAG 1 cut(s) 391
AgsI TTSAA 6 cut(s) 365, 375, 386, 531, 921, 986
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 9 cut(s) 23, 368, 508, 542, 586, 857, 908, 1062, 1142
AluI AGCT 9 cut(s) 23, 368, 508, 542, 586, 857, 908, 1062, 1142
Alw21I GWGCWC 3 cut(s) 766, 910, 1064
Alw26I GTCTC 3 cut(s) 51, 605, 815
AlwI GGATC 5 cut(s) 569, 659, 660, 710, 874
AlwNI CAGNNNCTG 1 cut(s) 572
Aor13HI TCCGGA 1 cut(s) 261
AoxI GGCC 1 cut(s) 976
ApeKI GCWGC 4 cut(s) 111, 409, 599, 854
ApoI RAATTY 1 cut(s) 944
Asp700I GAANNNNTTC 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 146
AsuNHI GCTAGC 1 cut(s) 586
BaeI ACNNNNGTAYC 2 cut(s) 297, 330
BalI TGGCCA 1 cut(s) 978
BanII GRGCYC 3 cut(s) 135, 910, 1064
Bbv12I GWGCWC 3 cut(s) 766, 910, 1064
BbvCI CCTCAGC 1 cut(s) 1092
BbvI GCAGC 4 cut(s) 123, 421, 611, 866
BccI CCATC 2 cut(s) 684, 830
BciVI GTATCC 1 cut(s) 325
BcoDI GTCTC 3 cut(s) 51, 605, 815
BfaI CTAG 7 cut(s) 299, 515, 587, 806, 833, 842, 1137
BfmI CTRYAG 3 cut(s) 407, 597, 999
BfrI CTTAAG 1 cut(s) 391
BfuAI ACCTGC 1 cut(s) 874
BfuI GTATCC 1 cut(s) 325
BglII AGATCT 1 cut(s) 325
BisI GCNGC 4 cut(s) 112, 410, 600, 855
BlpI GCTNAGC 2 cut(s) 34, 52
BlsI GCNGC 4 cut(s) 113, 411, 601, 856
BmiI GGNNCC 2 cut(s) 132, 266
BmrI ACTGGG 2 cut(s) 11, 821
BmsI GCATC 6 cut(s) 47, 712, 734, 795, 799, 1117
BmtI GCTAGC 1 cut(s) 590
BmuI ACTGGG 2 cut(s) 11, 821
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 1092
Bpu1102I GCTNAGC 2 cut(s) 34, 52
BpuEI CTTGAG 1 cut(s) 629
BsaAI YACGTR 3 cut(s) 673, 818, 902
BsaI GGTCTC 2 cut(s) 605, 815
BsaWI WCCGGW 1 cut(s) 261
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 776
Bse1I ACTGG 4 cut(s) 17, 561, 827, 969
BseAI TCCGGA 1 cut(s) 261
BseGI GGATG 7 cut(s) 38, 59, 237, 403, 453, 695, 786
BseLI CCNNNNNNNGG 2 cut(s) 621, 776
BseMII CTCAG 8 cut(s) 48, 66, 138, 372, 611, 674, 930, 1083
BseNI ACTGG 4 cut(s) 17, 561, 827, 969
BseRI GAGGAG 3 cut(s) 143, 735, 756
BseXI GCAGC 4 cut(s) 123, 421, 611, 866
BshFI GGCC 1 cut(s) 978
BsiHKAI GWGCWC 3 cut(s) 766, 910, 1064
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 1136
BslI CCNNNNNNNGG 2 cut(s) 621, 776
BsmAI GTCTC 3 cut(s) 51, 605, 815
BsmBI CGTCTC 1 cut(s) 51
BsmFI GGGAC 1 cut(s) 1136
BsnI GGCC 1 cut(s) 978
Bso31I GGTCTC 2 cut(s) 605, 815
Bsp1286I GDGCHC 4 cut(s) 135, 766, 910, 1064
Bsp13I TCCGGA 1 cut(s) 261
Bsp143I GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
Bsp1720I GCTNAGC 2 cut(s) 34, 52
BspANI GGCC 1 cut(s) 978
BspCNI CTCAG 8 cut(s) 47, 65, 137, 371, 612, 673, 931, 1084
BspEI TCCGGA 1 cut(s) 261
BspLI GGNNCC 2 cut(s) 132, 266
BspMAI CTGCAG 3 cut(s) 411, 601, 1003
BspMI ACCTGC 1 cut(s) 874
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 5 cut(s) 569, 659, 660, 710, 874
BspTI CTTAAG 1 cut(s) 391
BspTNI GGTCTC 2 cut(s) 605, 815
BsrI ACTGG 4 cut(s) 17, 561, 827, 969
BssMI GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
Bst4CI ACNGT 1 cut(s) 761
BstAFI CTTAAG 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBAI YACGTR 3 cut(s) 673, 818, 902
BstC8I GCNNGC 3 cut(s) 520, 540, 588
BstDEI CTNAG 8 cut(s) 34, 52, 124, 358, 620, 660, 939, 1092
BstF5I GGATG 7 cut(s) 38, 59, 237, 403, 453, 695, 786
BstKTI GATC 7 cut(s) 328, 577, 654, 668, 718, 869, 1108
BstMAI GTCTC 3 cut(s) 51, 605, 815
BstMBI GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
BstMWI GCNNNNNNNGC 2 cut(s) 596, 1019
BstNSI RCATGY 1 cut(s) 142
BstSFI CTRYAG 3 cut(s) 407, 597, 999
BstSNI TACGTA 2 cut(s) 673, 902
BstV1I GCAGC 4 cut(s) 123, 421, 611, 866
BstX2I RGATCY 2 cut(s) 325, 866
BstYI RGATCY 2 cut(s) 325, 866
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 1 cut(s) 978
BtsCI GGATG 7 cut(s) 38, 59, 237, 403, 453, 695, 786
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 5 cut(s) 121, 766, 834, 955, 1064
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 3 cut(s) 520, 540, 588
CaiI CAGNNNCTG 1 cut(s) 572
Csp6I GTAC 2 cut(s) 674, 1031
CviAII CATG 3 cut(s) 139, 648, 706
CviQI GTAC 2 cut(s) 674, 1031
DdeI CTNAG 8 cut(s) 34, 52, 124, 358, 620, 660, 939, 1092
DpnI GATC 7 cut(s) 327, 576, 653, 667, 717, 868, 1107
DpnII GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
EaeI YGGCCR 1 cut(s) 976
Ecl136II GAGCTC 2 cut(s) 908, 1062
Eco105I TACGTA 2 cut(s) 673, 902
Eco24I GRGCYC 3 cut(s) 135, 910, 1064
Eco31I GGTCTC 2 cut(s) 605, 815
Eco53kI GAGCTC 2 cut(s) 908, 1062
Eco57I CTGAAG 1 cut(s) 1035
EcoICRI GAGCTC 2 cut(s) 908, 1062
EcoRI GAATTC 1 cut(s) 944
EcoT22I ATGCAT 2 cut(s) 727, 788
EcoT38I GRGCYC 3 cut(s) 135, 910, 1064
Esp3I CGTCTC 1 cut(s) 51
FaeI CATG 3 cut(s) 142, 651, 709
FalI AAGNNNNNCTT 4 cut(s) 492, 524, 538, 570
FaqI GGGAC 1 cut(s) 1136
FatI CATG 3 cut(s) 138, 647, 705
FauNDI CATATG 1 cut(s) 238
Fnu4HI GCNGC 4 cut(s) 112, 410, 600, 855
FokI GGATG 7 cut(s) 25, 46, 244, 410, 460, 702, 773
FriOI GRGCYC 3 cut(s) 135, 910, 1064
Fsp4HI GCNGC 4 cut(s) 112, 410, 600, 855
FspBI CTAG 7 cut(s) 299, 515, 587, 806, 833, 842, 1137
GluI GCNGC 4 cut(s) 112, 410, 600, 855
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 1 cut(s) 978
HapII CCGG 1 cut(s) 262
Hin1II CATG 3 cut(s) 142, 651, 709
HincII GTYRAC 1 cut(s) 936
HindII GTYRAC 1 cut(s) 936
HindIII AAGCTT 1 cut(s) 506
HinfI GANTC 3 cut(s) 644, 932, 1114
HpaII CCGG 1 cut(s) 262
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 2 cut(s) 676, 936
Hpy188I TCNGA 5 cut(s) 127, 231, 424, 477, 912
Hpy188III TCNNGA 7 cut(s) 63, 216, 262, 299, 608, 719, 806
Hpy8I GTNNAC 2 cut(s) 676, 936
Hpy99I CGWCG 1 cut(s) 753
HpyAV CCTTC 3 cut(s) 92, 337, 537
HpyCH4III ACNGT 1 cut(s) 761
HpyCH4IV ACGT 4 cut(s) 592, 672, 817, 901
HpyF10VI GCNNNNNNNGC 2 cut(s) 596, 1019
HpyF3I CTNAG 8 cut(s) 34, 52, 124, 358, 620, 660, 939, 1092
HpySE526I ACGT 4 cut(s) 592, 672, 817, 901
Hsp92II CATG 3 cut(s) 142, 651, 709
Kpn2I TCCGGA 1 cut(s) 261
Kzo9I GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
LmnI GCTCC 3 cut(s) 92, 130, 769
Lsp1109I GCAGC 4 cut(s) 123, 421, 611, 866
LweI GCATC 6 cut(s) 47, 712, 734, 795, 799, 1117
MaeI CTAG 7 cut(s) 299, 515, 587, 806, 833, 842, 1137
MaeII ACGT 4 cut(s) 592, 672, 817, 901
MaeIII GTNAC 5 cut(s) 73, 116, 152, 568, 1051
MalI GATC 7 cut(s) 327, 576, 653, 667, 717, 868, 1107
MboI GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
MboII GAAGA 5 cut(s) 72, 202, 413, 546, 549
MflI RGATCY 2 cut(s) 325, 866
MhlI GDGCHC 4 cut(s) 135, 766, 910, 1064
MlsI TGGCCA 1 cut(s) 978
MluCI AATT 5 cut(s) 248, 381, 425, 625, 944
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 2 cut(s) 653, 941
Mox20I TGGCCA 1 cut(s) 978
Mph1103I ATGCAT 2 cut(s) 727, 788
MroI TCCGGA 1 cut(s) 261
MroXI GAANNNNTTC 1 cut(s) 225
MscI TGGCCA 1 cut(s) 978
MseI TTAA 3 cut(s) 251, 392, 888
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 857
MspCI CTTAAG 1 cut(s) 391
MspI CCGG 1 cut(s) 262
MwoI GCNNNNNNNGC 2 cut(s) 596, 1019
NdeI CATATG 1 cut(s) 238
NdeII GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 3 cut(s) 142, 651, 709
NlaIV GGNNCC 2 cut(s) 132, 266
NmuCI GTSAC 3 cut(s) 73, 116, 152
NsiI ATGCAT 2 cut(s) 727, 788
NspI RCATGY 1 cut(s) 142
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 1114
PflMI CCANNNNNTGG 1 cut(s) 776
PkrI GCNGC 4 cut(s) 113, 411, 601, 856
PleI GAGTC 2 cut(s) 652, 940
PpsI GAGTC 2 cut(s) 652, 940
Ppu21I YACGTR 3 cut(s) 673, 818, 902
Psp124BI GAGCTC 2 cut(s) 910, 1064
PspN4I GGNNCC 2 cut(s) 132, 266
PstI CTGCAG 3 cut(s) 411, 601, 1003
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 2 cut(s) 325, 866
PvuII CAGCTG 1 cut(s) 857
RsaI GTAC 2 cut(s) 675, 1032
RsaNI GTAC 2 cut(s) 674, 1031
SacI GAGCTC 2 cut(s) 910, 1064
SaqAI TTAA 3 cut(s) 251, 392, 888
SatI GCNGC 4 cut(s) 112, 410, 600, 855
Sau3AI GATC 7 cut(s) 325, 574, 651, 665, 715, 866, 1105
SchI GAGTC 2 cut(s) 653, 941
SduI GDGCHC 4 cut(s) 135, 766, 910, 1064
SfaNI GCATC 6 cut(s) 47, 712, 734, 795, 799, 1117
SfcI CTRYAG 3 cut(s) 407, 597, 999
SmlI CTYRAG 2 cut(s) 391, 608
SmoI CTYRAG 2 cut(s) 391, 608
SnaBI TACGTA 2 cut(s) 673, 902
Sse9I AATT 5 cut(s) 248, 381, 425, 625, 944
SspI AATATT 1 cut(s) 774
SspMI CTAG 7 cut(s) 299, 515, 587, 806, 833, 842, 1137
SstI GAGCTC 2 cut(s) 910, 1064
TaaI ACNGT 1 cut(s) 761
TaiI ACGT 4 cut(s) 595, 675, 820, 904
TaqI TCGA 4 cut(s) 350, 495, 642, 930
TaqII GACCGA 1 cut(s) 629
TasI AATT 5 cut(s) 248, 381, 425, 625, 944
TfiI GAWTC 1 cut(s) 1114
Tru1I TTAA 3 cut(s) 251, 392, 888
Tru9I TTAA 3 cut(s) 251, 392, 888
TscAI CASTG 5 cut(s) 121, 766, 834, 955, 1071
TseFI GTSAC 3 cut(s) 73, 116, 152
TseI GCWGC 4 cut(s) 111, 409, 599, 854
Tsp45I GTSAC 3 cut(s) 73, 116, 152
TspDTI ATGAA 6 cut(s) 17, 111, 120, 356, 414, 513
TspGWI ACGGA 1 cut(s) 689
TspRI CASTG 5 cut(s) 121, 766, 834, 955, 1071
Van91I CCANNNNNTGG 1 cut(s) 776
Vha464I CTTAAG 1 cut(s) 391
XapI RAATTY 1 cut(s) 944
XbaI TCTAGA 2 cut(s) 298, 805
XceI RCATGY 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 7 cut(s) 299, 515, 587, 806, 833, 842, 1137
Zsp2I ATGCAT 2 cut(s) 727, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.