Rmu_sc0000145.1_g000015

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000145.1
OG0017033
Physical Location & Seq
Forward (+)
97992 .. 100203
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 909 bp
atgttagcaaagcaggattggaggatactttctaatccccactcactgattgctaaattatacaaggctcgatactatccaaatcactctttttgggatgcagaattgggcgaagctccatctttctcttggagaagcattcttgaaagtagatctgtgcttgctgcagggagacaatggaaagttggtgatgggccaagattgagcctgcaatccccgttttccagaaaatgtattctgtccttgtattttcacttcttatcctctacttcatcaggaggcccttacctttttctgtggaagaaaatatggaatgcaagggtgccgggaagggtgaaaatctgtgtatggagaactgtgaaaaatatcctgcctacaagagagcagttcagtcataagggctacactagtccggggaattgtttgttatgtcaggaagtgtgtgagacaaacactcatgtctttcgccattgccctgttgctaaagaagctcttagaatagcagctatcccacttcaaagcagcatgccttgccatttcaaagattggatgttaactcaagggaagcatcttgacaacggaaattgggagaggttaattatgctggtctgggctctttggaagaacaggaacaagaagttgtggaatgaaatcactcaaacagcacaagccattgtagttggttcgttgacttggtatcagaaatttttacaagtgcaacaaatgcaaatgcaaagtcaagcagctaagcctagtaaatattggactccaccgccaactcaaaagcttaaaatcaatgttgatggagctttcctaccctctaagcatattgggggtataggtggagttatcagaagagaggatggctcttttgttgctggtttttctcagtcctttcaatatgtgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

302

Amino Acids

34.7

Weight (kDa)

9.95

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 95 - 163 1.5e-15 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 322
AciI CCGC 1 cut(s) 773
AcsI RAATTY 1 cut(s) 704
AgsI TTSAA 4 cut(s) 146, 518, 541, 899
AhlI ACTAGT 1 cut(s) 407
AluBI AGCT 6 cut(s) 116, 491, 506, 746, 787, 809
AluI AGCT 6 cut(s) 116, 491, 506, 746, 787, 809
Alw26I GTCTC 2 cut(s) 166, 440
AoxI GGCC 2 cut(s) 194, 280
ApeKI GCWGC 4 cut(s) 164, 503, 522, 743
ApoI RAATTY 1 cut(s) 704
ArsI GACNNNNNNTTYG 2 cut(s) 721, 753
AspS9I GGNCC 2 cut(s) 194, 281
AsuC2I CCSGG 2 cut(s) 327, 414
AsuHPI GGTGA 2 cut(s) 200, 346
BanI GGYRCC 1 cut(s) 322
BanII GRGCYC 1 cut(s) 616
BarI GAAGNNNNNNTAC 2 cut(s) 239, 271
BbvI GCAGC 4 cut(s) 151, 515, 534, 755
BccI CCATC 4 cut(s) 127, 185, 797, 857
BciVI GTATCC 1 cut(s) 18
BcnI CCSGG 2 cut(s) 327, 414
BcoDI GTCTC 2 cut(s) 166, 440
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 2 cut(s) 408, 753
BfmI CTRYAG 1 cut(s) 165
BfuI GTATCC 1 cut(s) 18
BglII AGATCT 1 cut(s) 152
BisI GCNGC 4 cut(s) 165, 504, 523, 744
BlpI GCTNAGC 1 cut(s) 747
BlsI GCNGC 4 cut(s) 166, 505, 524, 745
Bme1390I CCNGG 2 cut(s) 327, 414
BmgT120I GGNCC 2 cut(s) 194, 281
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 2 cut(s) 327, 414
BmsI GCATC 2 cut(s) 88, 577
BplI GAGNNNNNCTC 2 cut(s) 851, 883
Bpu1102I GCTNAGC 1 cut(s) 747
BpuEI CTTGAG 1 cut(s) 543
BpuMI CCSGG 2 cut(s) 327, 414
BsaJI CCNNGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 3 cut(s) 103, 555, 868
BseMI GCAATG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 902
BseXI GCAGC 4 cut(s) 151, 515, 534, 755
BshFI GGCC 2 cut(s) 196, 282
BshNI GGYRCC 1 cut(s) 322
BsiSI CCGG 2 cut(s) 326, 413
BsmAI GTCTC 2 cut(s) 166, 440
BsmI GAATGC 2 cut(s) 138, 319
BsnI GGCC 2 cut(s) 196, 282
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 1 cut(s) 152
Bsp1720I GCTNAGC 1 cut(s) 747
BspACI CCGC 1 cut(s) 773
BspANI GGCC 2 cut(s) 196, 282
BspCNI CTCAG 1 cut(s) 901
BspLI GGNNCC 1 cut(s) 324
BspMAI CTGCAG 1 cut(s) 169
BspT107I GGYRCC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 1 cut(s) 850
BstAPI GCANNNNNTGC 2 cut(s) 531, 724
BstC8I GCNNGC 3 cut(s) 162, 209, 527
BstDEI CTNAG 4 cut(s) 494, 747, 822, 888
BstF5I GGATG 3 cut(s) 103, 555, 868
BstKTI GATC 1 cut(s) 155
BstMAI GTCTC 2 cut(s) 166, 440
BstMBI GATC 1 cut(s) 152
BstMWI GCNNNNNNNGC 3 cut(s) 488, 531, 724
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 2 cut(s) 325, 412
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 4 cut(s) 151, 515, 534, 755
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 2 cut(s) 196, 282
BtsCI GGATG 3 cut(s) 103, 555, 868
BtsIMutI CAGTG 1 cut(s) 44
Cac8I GCNNGC 3 cut(s) 162, 209, 527
Cfr13I GGNCC 2 cut(s) 194, 281
CviAII CATG 2 cut(s) 458, 526
DdeI CTNAG 4 cut(s) 494, 747, 822, 888
DpnI GATC 1 cut(s) 154
DpnII GATC 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 850
EarI CTCTTC 1 cut(s) 850
Eco24I GRGCYC 1 cut(s) 616
EcoO109I RGGNCCY 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 616
FaeI CATG 2 cut(s) 461, 529
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FatI CATG 2 cut(s) 457, 525
Fnu4HI GCNGC 4 cut(s) 165, 504, 523, 744
FokI GGATG 3 cut(s) 110, 562, 875
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 4 cut(s) 165, 504, 523, 744
FspBI CTAG 2 cut(s) 408, 753
GluI GCNGC 4 cut(s) 165, 504, 523, 744
HaeIII GGCC 2 cut(s) 196, 282
HapII CCGG 2 cut(s) 326, 413
Hin1II CATG 2 cut(s) 461, 529
HincII GTYRAC 2 cut(s) 555, 690
HindII GTYRAC 2 cut(s) 555, 690
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 1 cut(s) 766
HpaI GTTAAC 1 cut(s) 555
HpaII CCGG 2 cut(s) 326, 413
HphI GGTGA 2 cut(s) 200, 346
Hpy166II GTNNAC 2 cut(s) 555, 690
Hpy188I TCNGA 2 cut(s) 702, 854
Hpy188III TCNNGA 5 cut(s) 143, 225, 276, 434, 572
Hpy8I GTNNAC 2 cut(s) 555, 690
HpyAV CCTTC 1 cut(s) 324
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4V TGCA 7 cut(s) 101, 167, 211, 317, 718, 727, 733
HpyF10VI GCNNNNNNNGC 3 cut(s) 488, 531, 724
HpyF3I CTNAG 4 cut(s) 494, 747, 822, 888
Hsp92II CATG 2 cut(s) 461, 529
KspAI GTTAAC 1 cut(s) 555
Kzo9I GATC 1 cut(s) 152
LmnI GCTCC 2 cut(s) 121, 806
Lsp1109I GCAGC 4 cut(s) 151, 515, 534, 755
LweI GCATC 2 cut(s) 88, 577
MaeI CTAG 2 cut(s) 408, 753
MalI GATC 1 cut(s) 154
MboI GATC 1 cut(s) 152
MboII GAAGA 3 cut(s) 313, 634, 867
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 6 cut(s) 56, 104, 418, 583, 597, 704
MlyI GAGTC 1 cut(s) 760
MnlI CCTC 6 cut(s) 15, 272, 274, 585, 829, 853
MseI TTAA 3 cut(s) 554, 596, 789
MspI CCGG 2 cut(s) 326, 413
MspR9I CCNGG 2 cut(s) 327, 414
Mva1269I GAATGC 2 cut(s) 138, 319
MwoI GCNNNNNNNGC 3 cut(s) 488, 531, 724
NciI CCSGG 2 cut(s) 327, 414
NdeII GATC 1 cut(s) 152
NlaIII CATG 2 cut(s) 461, 529
NlaIV GGNNCC 1 cut(s) 324
NspI RCATGY 1 cut(s) 529
PaeI GCATGC 1 cut(s) 529
PctI GAATGC 2 cut(s) 138, 319
PkrI GCNGC 4 cut(s) 166, 505, 524, 745
PleI GAGTC 1 cut(s) 760
PpsI GAGTC 1 cut(s) 760
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 2 cut(s) 194, 281
PstI CTGCAG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 152
SaqAI TTAA 3 cut(s) 554, 596, 789
SatI GCNGC 4 cut(s) 165, 504, 523, 744
Sau3AI GATC 1 cut(s) 152
Sau96I GGNCC 2 cut(s) 194, 281
SchI GAGTC 1 cut(s) 760
ScrFI CCNGG 2 cut(s) 327, 414
SduI GDGCHC 1 cut(s) 616
SetI ASST 9 cut(s) 118, 291, 493, 508, 596, 748, 789, 811, 844
SfaNI GCATC 2 cut(s) 88, 577
SfcI CTRYAG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 558
SmoI CTYRAG 1 cut(s) 558
SpeI ACTAGT 1 cut(s) 407
SphI GCATGC 1 cut(s) 529
Sse9I AATT 6 cut(s) 56, 104, 418, 583, 597, 704
SsiI CCGC 1 cut(s) 773
SspI AATATT 1 cut(s) 761
SspMI CTAG 2 cut(s) 408, 753
StyD4I CCNGG 2 cut(s) 325, 412
TaaI ACNGT 1 cut(s) 358
TaqI TCGA 1 cut(s) 70
TasI AATT 6 cut(s) 56, 104, 418, 583, 597, 704
Tru1I TTAA 3 cut(s) 554, 596, 789
Tru9I TTAA 3 cut(s) 554, 596, 789
TscAI CASTG 1 cut(s) 51
TseI GCWGC 4 cut(s) 164, 503, 522, 743
TspDTI ATGAA 2 cut(s) 261, 663
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 1 cut(s) 51
XapI RAATTY 1 cut(s) 704
XceI RCATGY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XspI CTAG 2 cut(s) 408, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.