Rh1CG225300

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0017033
Physical Location & Seq
Reverse (-)
47726765 .. 47733442
6678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG225300.1

Sequence Viewer

Length: 354 bp
ATGCAGAAGTTACAGCCACAAGACAAAGCAGCCAAACCGGGTAAACATTGGACTCCACCATCAAGACAACAGCTCAAAATCAATGTTGATGGAGCTTTCTTGCCTTCTAAAGATATTGGAGGCATTGGTGGAGTGATCAAAAGAAAAGATAGTTTTTTTGTAGCTAGTTTTTCAAAGTGTGGACATCATGTTTCACCTCCAAAGCACGTGGAGCTACTAGCAATCCGTGAGGGCATTGATTTTCTACGGCAGCGTAGCCTCCAAGAAGCATGCCTTGAGAATAATTGCCAGTTGAGGAGCAAGCAGTTGACAGGTGAAACTGTGAAAGGAGATGGACCCTTTGCCTTTGGTTAG

Protein Analysis

117

Amino Acids

12.89

Weight (kDa)

9.62

Isoelectric Point (pI)

52.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 28 - 108 9.3e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcvI CACGTG 1 cut(s) 208
AgsI TTSAA 1 cut(s) 174
AluBI AGCT 4 cut(s) 73, 95, 164, 214
AluI AGCT 4 cut(s) 73, 95, 164, 214
ApeKI GCWGC 2 cut(s) 29, 250
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 2 cut(s) 186, 326
AvaII GGWCC 1 cut(s) 335
BbrPI CACGTG 1 cut(s) 208
BbvI GCAGC 2 cut(s) 41, 262
BccI CCATC 3 cut(s) 67, 83, 326
BceAI ACGGC 1 cut(s) 263
BclI TGATCA 1 cut(s) 135
BcnI CCSGG 1 cut(s) 39
BfaI CTAG 2 cut(s) 165, 218
BisI GCNGC 2 cut(s) 30, 251
BlsI GCNGC 2 cut(s) 31, 252
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 289
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 1 cut(s) 310
BseXI GCAGC 2 cut(s) 41, 262
BsiSI CCGG 1 cut(s) 38
Bsp143I GATC 1 cut(s) 135
BspLI GGNNCC 1 cut(s) 337
BsrI ACTGG 1 cut(s) 289
BssMI GATC 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 322
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 271, 302
BstKTI GATC 1 cut(s) 138
BstMBI GATC 1 cut(s) 135
BstMWI GCNNNNNNNGC 1 cut(s) 211
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 41, 262
Cac8I GCNNGC 2 cut(s) 271, 302
Cfr13I GGNCC 1 cut(s) 335
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 2 cut(s) 188, 270
CviJI RGCY 7 cut(s) 16, 32, 73, 95, 164, 214, 258
CviKI_1 RGCY 7 cut(s) 16, 32, 73, 95, 164, 214, 258
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 335
Eco72I CACGTG 1 cut(s) 208
FaeI CATG 2 cut(s) 191, 273
FaiI YATR 2 cut(s) 189, 271
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FatI CATG 2 cut(s) 187, 269
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 2 cut(s) 30, 251
Fsp4HI GCNGC 2 cut(s) 30, 251
FspBI CTAG 2 cut(s) 165, 218
GluI GCNGC 2 cut(s) 30, 251
HapII CCGG 1 cut(s) 38
Hin1II CATG 2 cut(s) 191, 273
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 1 cut(s) 52
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 186, 326
Hpy166II GTNNAC 3 cut(s) 44, 182, 309
Hpy188III TCNNGA 1 cut(s) 63
Hpy8I GTNNAC 3 cut(s) 44, 182, 309
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 211
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 2 cut(s) 191, 273
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 1 cut(s) 135
LmnI GCTCC 3 cut(s) 92, 211, 297
LpnPI CCDG 3 cut(s) 51, 297, 302
Lsp1109I GCAGC 2 cut(s) 41, 262
MaeI CTAG 2 cut(s) 165, 218
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 9
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MluCI AATT 1 cut(s) 283
MlyI GAGTC 1 cut(s) 46
MnlI CCTC 5 cut(s) 113, 207, 223, 269, 288
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 39
NdeII GATC 1 cut(s) 135
NlaIII CATG 2 cut(s) 191, 273
NlaIV GGNNCC 1 cut(s) 337
NspI RCATGY 1 cut(s) 273
PaeI GCATGC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 31, 252
PleI GAGTC 1 cut(s) 46
PmaCI CACGTG 1 cut(s) 208
PmlI CACGTG 1 cut(s) 208
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 208
PspCI CACGTG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 335
SatI GCNGC 2 cut(s) 30, 251
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 39
SetI ASST 7 cut(s) 75, 97, 166, 199, 210, 216, 316
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SphI GCATGC 1 cut(s) 273
Sse9I AATT 1 cut(s) 283
SspMI CTAG 2 cut(s) 165, 218
StyD4I CCNGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 1 cut(s) 210
TasI AATT 1 cut(s) 283
TseI GCWGC 2 cut(s) 29, 250
TspGWI ACGGA 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 335
XceI RCATGY 1 cut(s) 273
XspI CTAG 2 cut(s) 165, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.