Rh4DG325500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017033
Physical Location & Seq
Reverse (-)
54999818 .. 55000471
654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG325500.1

Sequence Viewer

Length: 654 bp
ATGCTCCTTTGGGCTCTCTGGAAGAATAGAAACAGTAAGTTGTGGCACAATACGTGCATCAATGCTCATGCAATTGTCGTTACAACGTTCTCTTGGTATGAACAGTTTATACAAGTGCAGAAGCCTTCTGTGCATGAACCAGCTGCCAAACCGAGTAAACATTGGTCTCCACCATCTAGCAATCAGCTCAAAATCAATGTTGATGGAGCTTTCTTGCCTTCAAAGGACTTTGGAGGTATTGGTGGAGTAATCCGACGAAGTGATGGCTCTTTTGTAGCTGCATTCTCCAAACTTGTGCAGCATGTCTCATCTCTAATGCAAGTGGAACTATTAGCAATTAGAGAAGGCCTGGACTTTCTCAAGCAGAGAGGCCTTCACGATGTATGCCTTGAAAGTGACTGTCAAGGAGTGATTCAAGACATTAATACGGGGGATTGTGACTTGATTGAAAATGCGAACCTGCTGCTCGACATCCAACAGTGTAGTAAGTTTGTGCATAATTTGAAATTCTCCTTTTCCCCTAGGACTGCCAATTTTGTGGCACATAGATTAGCAGCTTTTGCTTATGAATCCAATACTACTAATGTATGGATAGACCTTGCTCCAGTAAATATTCTGGATGCTCTAGCTTCTGATTGTATTCGATCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

217

Amino Acids

24.24

Weight (kDa)

6.69

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 66 - 188 1.4e-21 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 468
AclI AACGTT 1 cut(s) 86
AcsI RAATTY 1 cut(s) 506
AgsI TTSAA 5 cut(s) 222, 392, 416, 449, 505
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 6 cut(s) 143, 187, 209, 278, 557, 629
AluI AGCT 6 cut(s) 143, 187, 209, 278, 557, 629
Alw26I GTCTC 2 cut(s) 171, 310
AoxI GGCC 2 cut(s) 346, 370
ApeKI GCWGC 5 cut(s) 143, 278, 298, 463, 554
ApoI RAATTY 1 cut(s) 506
AseI ATTAAT 1 cut(s) 423
AspA2I CCTAGG 1 cut(s) 521
AvrII CCTAGG 1 cut(s) 521
BanII GRGCYC 1 cut(s) 16
BbvI GCAGC 5 cut(s) 130, 265, 310, 450, 566
BccI CCATC 3 cut(s) 181, 197, 257
BcgI CGANNNNNNTGC 2 cut(s) 445, 479
BciT130I CCWGG 1 cut(s) 350
BcoDI GTCTC 2 cut(s) 171, 310
BfaI CTAG 3 cut(s) 177, 522, 626
BfuAI ACCTGC 1 cut(s) 468
BisI GCNGC 5 cut(s) 144, 279, 299, 464, 555
BlnI CCTAGG 1 cut(s) 521
BlsI GCNGC 5 cut(s) 145, 280, 300, 465, 556
Bme1390I CCNGG 1 cut(s) 350
BmrFI CCNGG 1 cut(s) 350
BmsI GCATC 2 cut(s) 66, 610
BpmI CTGGAG 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 54
BsaI GGTCTC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 521
Bse1I ACTGG 1 cut(s) 605
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 521
BseGI GGATG 2 cut(s) 471, 625
BseNI ACTGG 1 cut(s) 605
BseXI GCAGC 5 cut(s) 130, 265, 310, 450, 566
BsgI GTGCAG 2 cut(s) 137, 317
Bsh1285I CGRYCG 1 cut(s) 647
BshFI GGCC 2 cut(s) 348, 372
BsiEI CGRYCG 1 cut(s) 647
BsmAI GTCTC 2 cut(s) 171, 310
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 2 cut(s) 348, 372
Bso31I GGTCTC 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 1 cut(s) 644
BspANI GGCC 2 cut(s) 348, 372
BspMI ACCTGC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 171
BsrI ACTGG 1 cut(s) 605
BssECI CCNNGG 1 cut(s) 521
BssMI GATC 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 521
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 4 cut(s) 35, 105, 401, 480
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 54
BstF5I GGATG 2 cut(s) 471, 625
BstKTI GATC 1 cut(s) 647
BstMAI GTCTC 2 cut(s) 171, 310
BstMBI GATC 1 cut(s) 644
BstMCI CGRYCG 1 cut(s) 647
BstMWI GCNNNNNNNGC 2 cut(s) 130, 560
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 348
BstV1I GCAGC 5 cut(s) 130, 265, 310, 450, 566
BstXI CCANNNNNNTGG 1 cut(s) 538
BsuRI GGCC 2 cut(s) 348, 372
BtsCI GGATG 2 cut(s) 471, 625
BtsIMutI CAGTG 1 cut(s) 485
BveI ACCTGC 1 cut(s) 468
CviAII CATG 3 cut(s) 68, 134, 302
DpnI GATC 1 cut(s) 646
DpnII GATC 1 cut(s) 644
Eco130I CCWWGG 1 cut(s) 521
Eco147I AGGCCT 2 cut(s) 348, 372
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 521
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 521
FaeI CATG 3 cut(s) 71, 137, 305
FatI CATG 3 cut(s) 67, 133, 301
Fnu4HI GCNGC 5 cut(s) 144, 279, 299, 464, 555
FokI GGATG 2 cut(s) 458, 632
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 5 cut(s) 144, 279, 299, 464, 555
FspBI CTAG 3 cut(s) 177, 522, 626
GluI GCNGC 5 cut(s) 144, 279, 299, 464, 555
GsuI CTGGAG 1 cut(s) 588
HaeIII GGCC 2 cut(s) 348, 372
Hin1II CATG 3 cut(s) 71, 137, 305
HinfI GANTC 2 cut(s) 412, 569
Hpy166II GTNNAC 1 cut(s) 158
Hpy188I TCNGA 2 cut(s) 254, 634
Hpy188III TCNNGA 4 cut(s) 19, 377, 416, 617
Hpy8I GTNNAC 1 cut(s) 158
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 4 cut(s) 135, 228, 338, 383
HpyCH4III ACNGT 4 cut(s) 35, 105, 401, 480
HpyCH4IV ACGT 2 cut(s) 53, 86
HpyCH4V TGCA 8 cut(s) 57, 71, 118, 133, 281, 298, 319, 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 560
HpySE526I ACGT 2 cut(s) 53, 86
Hsp92II CATG 3 cut(s) 71, 137, 305
Kzo9I GATC 1 cut(s) 644
LmnI GCTCC 3 cut(s) 9, 206, 607
LpnPI CCDG 7 cut(s) 4, 153, 335, 362, 473, 602, 618
Lsp1109I GCAGC 5 cut(s) 130, 265, 310, 450, 566
LweI GCATC 2 cut(s) 66, 610
MaeI CTAG 3 cut(s) 177, 522, 626
MaeII ACGT 2 cut(s) 53, 86
MaeIII GTNAC 3 cut(s) 79, 395, 437
MalI GATC 1 cut(s) 646
MboI GATC 1 cut(s) 644
MboII GAAGA 1 cut(s) 34
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 6 cut(s) 72, 336, 499, 506, 532, 649
MmeI TCCRAC 2 cut(s) 277, 499
MnlI CCTC 2 cut(s) 227, 362
MseI TTAA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 1 cut(s) 350
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 130, 560
NdeII GATC 1 cut(s) 644
NlaIII CATG 3 cut(s) 71, 137, 305
NmuCI GTSAC 2 cut(s) 395, 437
NspI RCATGY 1 cut(s) 305
PceI AGGCCT 2 cut(s) 348, 372
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 412, 569
PkrI GCNGC 5 cut(s) 145, 280, 300, 465, 556
Ple19I CGATCG 1 cut(s) 647
Ppu21I YACGTR 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 423
Psp1406I AACGTT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 348
PspGI CCWGG 1 cut(s) 348
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PvuI CGATCG 1 cut(s) 647
PvuII CAGCTG 1 cut(s) 143
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 5 cut(s) 144, 279, 299, 464, 555
Sau3AI GATC 1 cut(s) 644
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 2 cut(s) 66, 610
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 6 cut(s) 72, 336, 499, 506, 532, 649
SseBI AGGCCT 2 cut(s) 348, 372
SspI AATATT 1 cut(s) 613
SspMI CTAG 3 cut(s) 177, 522, 626
StuI AGGCCT 2 cut(s) 348, 372
StyD4I CCNGG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 521
TaaI ACNGT 4 cut(s) 35, 105, 401, 480
TaiI ACGT 2 cut(s) 56, 89
TaqI TCGA 3 cut(s) 468, 643, 647
TasI AATT 6 cut(s) 72, 336, 499, 506, 532, 649
TfiI GAWTC 2 cut(s) 412, 569
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 485
TseFI GTSAC 2 cut(s) 395, 437
TseI GCWGC 5 cut(s) 143, 278, 298, 463, 554
Tsp45I GTSAC 2 cut(s) 395, 437
TspDTI ATGAA 3 cut(s) 114, 150, 582
TspRI CASTG 1 cut(s) 485
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 305
XmaJI CCTAGG 1 cut(s) 521
XspI CTAG 3 cut(s) 177, 522, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.