Rorug05G0147500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017033
Physical Location & Seq
Forward (+)
13724919 .. 13726083
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0147500.1

Sequence Viewer

Length: 519 bp
ATGGCTATGACTAAACCTCATTACCAAGCAGAGAAAGAAGAAATCATAAAGCTGGAGGGAGATAACTACCGTATCAAACCACGAGGACTTAATATTGTATGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAGGGACTGAGGATCCTGCAGAGCTGGTACAGGTCTCATGGCTGGAAGTAACAGGCTCAGTTGATGAGTTGACACCCGGGAAGGGGTATGATCTGACCTTCGAGGTGGGGCTGGCAGGAGATGCATTTGGTTGGAAAGACATACAAGTGTTCTTGATGGCAAAGGTAGGGAAAAAGGGAAGGTACAAATGGACAAGAGTTAAACTCGATCAGGATAGGAATAACGAGATGAAAGTAACAATTCCTGATAACCAGAAGCTTAGCATTGATGTTCCAGCTAATACAAGGGACACCACCCTTCATTTTGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAGGGAGGCTTGAAGATTTATAATGCGAGAGTAACTGCGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

172

Amino Acids

19.62

Weight (kDa)

8.63

Isoelectric Point (pI)

9.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 22 - 164 7.7e-22 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AclWI GGATC 2 cut(s) 143, 156
AfaI GTAC 2 cut(s) 165, 320
AfiI CCNNNNNNNGG 3 cut(s) 137, 218, 219
AgsI TTSAA 1 cut(s) 484
AluBI AGCT 4 cut(s) 52, 160, 394, 413
AluI AGCT 4 cut(s) 52, 160, 394, 413
Alw26I GTCTC 1 cut(s) 175
AlwI GGATC 2 cut(s) 143, 156
Ama87I CYCGRG 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 213, 214
AvaI CYCGRG 1 cut(s) 212
BaeI ACNNNNGTAYC 2 cut(s) 55, 88
BamHI GGATCC 1 cut(s) 148
BauI CACGAG 1 cut(s) 81
BccI CCATC 1 cut(s) 286
BcnI CCSGG 2 cut(s) 213, 214
BcoDI GTCTC 1 cut(s) 175
BfmI CTRYAG 1 cut(s) 153
BlpI GCTNAGC 1 cut(s) 395
Bme1390I CCNGG 2 cut(s) 213, 214
BmeT110I CYCGRG 1 cut(s) 212
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 2 cut(s) 213, 214
BmsI GCATC 1 cut(s) 247
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpmI CTGGAG 1 cut(s) 74
Bpu1102I GCTNAGC 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 213, 214
BsaI GGTCTC 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 212
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 218, 219
Bse1I ACTGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 112
BseDI CCNNGG 1 cut(s) 212
BseLI CCNNNNNNNGG 3 cut(s) 137, 218, 219
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 135, 207
BseNI ACTGG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 212
BsiSI CCGG 1 cut(s) 213
BslFI GGGAC 2 cut(s) 154, 437
BslI CCNNNNNNNGG 3 cut(s) 137, 218, 219
BsmAI GTCTC 1 cut(s) 175
BsmFI GGGAC 2 cut(s) 154, 437
Bso31I GGTCTC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 212
Bsp143I GATC 3 cut(s) 148, 226, 343
Bsp1720I GCTNAGC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 136, 206
BspLI GGNNCC 1 cut(s) 150
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 2 cut(s) 143, 156
BspTNI GGTCTC 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 112
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 3 cut(s) 148, 226, 343
BssSI CACGAG 1 cut(s) 81
Bst2BI CACGAG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 3 cut(s) 144, 193, 395
BstKTI GATC 3 cut(s) 151, 229, 346
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 3 cut(s) 148, 226, 343
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSCI CCNGG 2 cut(s) 211, 212
BstSFI CTRYAG 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 249
Cfr9I CCCGGG 1 cut(s) 212
Csp6I GTAC 2 cut(s) 164, 319
CviAII CATG 1 cut(s) 174
CviQI GTAC 2 cut(s) 164, 319
DdeI CTNAG 3 cut(s) 144, 193, 395
DpnI GATC 3 cut(s) 150, 228, 345
DpnII GATC 3 cut(s) 148, 226, 343
Eco31I GGTCTC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 212
EcoT22I ATGCAT 1 cut(s) 262
FaeI CATG 1 cut(s) 177
FaiI YATR 8 cut(s) 8, 47, 100, 175, 225, 278, 450, 492
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 2 cut(s) 154, 437
FatI CATG 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 74
HapII CCGG 1 cut(s) 213
Hin1II CATG 1 cut(s) 177
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 1 cut(s) 511
HpaII CCGG 1 cut(s) 213
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 3 cut(s) 289, 347, 380
Hpy8I GTNNAC 1 cut(s) 207
HpyAV CCTTC 5 cut(s) 211, 244, 309, 443, 466
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 2 cut(s) 155, 260
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 3 cut(s) 144, 193, 395
Hsp92II CATG 1 cut(s) 177
Kzo9I GATC 3 cut(s) 148, 226, 343
LweI GCATC 1 cut(s) 247
MaeIII GTNAC 3 cut(s) 184, 370, 502
MalI GATC 3 cut(s) 150, 228, 345
MboI GATC 3 cut(s) 148, 226, 343
MboII GAAGA 2 cut(s) 50, 496
MflI RGATCY 1 cut(s) 148
MluCI AATT 1 cut(s) 375
MmeI TCCRAC 1 cut(s) 248
MnlI CCTC 6 cut(s) 27, 49, 77, 139, 232, 470
Mph1103I ATGCAT 1 cut(s) 262
MseI TTAA 2 cut(s) 90, 336
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 2 cut(s) 213, 214
MwoI GCNNNNNNNGC 1 cut(s) 257
NciI CCSGG 2 cut(s) 213, 214
NdeII GATC 3 cut(s) 148, 226, 343
NlaIII CATG 1 cut(s) 177
NlaIV GGNNCC 1 cut(s) 150
NsiI ATGCAT 1 cut(s) 262
PsiI TTATAA 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 150
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 148
RsaI GTAC 2 cut(s) 165, 320
RsaNI GTAC 2 cut(s) 164, 319
RseI CAYNNNNRTG 1 cut(s) 281
SaqAI TTAA 2 cut(s) 90, 336
Sau3AI GATC 3 cut(s) 148, 226, 343
ScrFI CCNGG 2 cut(s) 213, 214
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 153
SmaI CCCGGG 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 281
Sse9I AATT 1 cut(s) 375
SspI AATATT 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 211, 212
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 2 cut(s) 237, 342
TasI AATT 1 cut(s) 375
Tru1I TTAA 2 cut(s) 90, 336
Tru9I TTAA 2 cut(s) 90, 336
TspDTI ATGAA 4 cut(s) 126, 380, 425, 465
TspMI CCCGGG 1 cut(s) 212
XmaI CCCGGG 1 cut(s) 212
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.