Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 161 |
| Acc36I | ACCTGC | 1 cut(s) | 87 |
| AciI | CCGC | 2 cut(s) | 38, 787 |
| AclWI | GGATC | 2 cut(s) | 268, 493 |
| AcoI | YGGCCR | 1 cut(s) | 586 |
| AcsI | RAATTY | 1 cut(s) | 636 |
| AcyI | GRCGYC | 1 cut(s) | 158 |
| AfaI | GTAC | 2 cut(s) | 101, 271 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 109 |
| AgsI | TTSAA | 6 cut(s) | 641, 654, 663, 668, 710, 855 |
| AjnI | CCWGG | 1 cut(s) | 394 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 835, 867 |
| AluBI | AGCT | 3 cut(s) | 209, 592, 801 |
| AluI | AGCT | 3 cut(s) | 209, 592, 801 |
| Alw21I | GWGCWC | 1 cut(s) | 406 |
| Alw26I | GTCTC | 1 cut(s) | 149 |
| Alw44I | GTGCAC | 1 cut(s) | 402 |
| AlwI | GGATC | 2 cut(s) | 268, 493 |
| Ama87I | CYCGRG | 1 cut(s) | 368 |
| AoxI | GGCC | 2 cut(s) | 334, 586 |
| ApaLI | GTGCAC | 1 cut(s) | 402 |
| ApeKI | GCWGC | 5 cut(s) | 293, 531, 701, 911, 925 |
| ApoI | RAATTY | 1 cut(s) | 636 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 201 |
| AspS9I | GGNCC | 1 cut(s) | 137 |
| AsuHPI | GGTGA | 2 cut(s) | 187, 384 |
| AvaI | CYCGRG | 1 cut(s) | 368 |
| AvaII | GGWCC | 1 cut(s) | 137 |
| AxyI | CCTNAGG | 1 cut(s) | 309 |
| BaeGI | GKGCMC | 1 cut(s) | 406 |
| BalI | TGGCCA | 1 cut(s) | 588 |
| BbsI | GAAGAC | 1 cut(s) | 675 |
| Bbv12I | GWGCWC | 1 cut(s) | 406 |
| BbvI | GCAGC | 5 cut(s) | 305, 543, 713, 912, 923 |
| BccI | CCATC | 1 cut(s) | 237 |
| BceAI | ACGGC | 2 cut(s) | 159, 281 |
| BciT130I | CCWGG | 1 cut(s) | 396 |
| BclI | TGATCA | 1 cut(s) | 511 |
| BcoDI | GTCTC | 1 cut(s) | 149 |
| BfaI | CTAG | 4 cut(s) | 129, 147, 210, 729 |
| BfmI | CTRYAG | 1 cut(s) | 909 |
| BfuAI | ACCTGC | 1 cut(s) | 87 |
| BisI | GCNGC | 5 cut(s) | 294, 532, 702, 912, 926 |
| BlsI | GCNGC | 5 cut(s) | 295, 533, 703, 913, 927 |
| Bme1390I | CCNGG | 1 cut(s) | 396 |
| Bme18I | GGWCC | 1 cut(s) | 137 |
| BmeT110I | CYCGRG | 1 cut(s) | 368 |
| BmgT120I | GGNCC | 1 cut(s) | 137 |
| BmiI | GGNNCC | 1 cut(s) | 365 |
| BmrFI | CCNGG | 1 cut(s) | 396 |
| BmsI | GCATC | 2 cut(s) | 544, 857 |
| BoxI | GACNNNNGTC | 2 cut(s) | 388, 752 |
| BpiI | GAAGAC | 1 cut(s) | 675 |
| BpuEI | CTTGAG | 1 cut(s) | 849 |
| BsaHI | GRCGYC | 1 cut(s) | 158 |
| BsaJI | CCNNGG | 1 cut(s) | 395 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 33 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 109 |
| Bse118I | RCCGGY | 1 cut(s) | 336 |
| Bse1I | ACTGG | 1 cut(s) | 564 |
| Bse21I | CCTNAGG | 1 cut(s) | 309 |
| BseBI | CCWGG | 1 cut(s) | 396 |
| BseDI | CCNNGG | 1 cut(s) | 395 |
| BseGI | GGATG | 3 cut(s) | 248, 795, 930 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 109 |
| BseMII | CTCAG | 3 cut(s) | 323, 503, 515 |
| BseNI | ACTGG | 1 cut(s) | 564 |
| BseRI | GAGGAG | 1 cut(s) | 600 |
| BseSI | GKGCMC | 1 cut(s) | 406 |
| BseXI | GCAGC | 5 cut(s) | 305, 543, 713, 912, 923 |
| BseYI | CCCAGC | 1 cut(s) | 631 |
| BshFI | GGCC | 2 cut(s) | 336, 588 |
| BsiHKAI | GWGCWC | 1 cut(s) | 406 |
| BsiHKCI | CYCGRG | 1 cut(s) | 368 |
| BsiSI | CCGG | 1 cut(s) | 337 |
| BslFI | GGGAC | 1 cut(s) | 289 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 109 |
| BsmAI | GTCTC | 1 cut(s) | 149 |
| BsmBI | CGTCTC | 1 cut(s) | 149 |
| BsmFI | GGGAC | 1 cut(s) | 289 |
| BsnI | GGCC | 2 cut(s) | 336, 588 |
| BsoBI | CYCGRG | 1 cut(s) | 368 |
| Bsp1286I | GDGCHC | 1 cut(s) | 406 |
| Bsp143I | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| BspACI | CCGC | 2 cut(s) | 38, 787 |
| BspANI | GGCC | 2 cut(s) | 336, 588 |
| BspCNI | CTCAG | 3 cut(s) | 322, 502, 514 |
| BspLI | GGNNCC | 1 cut(s) | 365 |
| BspMAI | CTGCAG | 1 cut(s) | 913 |
| BspMI | ACCTGC | 1 cut(s) | 87 |
| BspPI | GGATC | 2 cut(s) | 268, 493 |
| BsrFI | RCCGGY | 1 cut(s) | 336 |
| BsrI | ACTGG | 1 cut(s) | 564 |
| BssAI | RCCGGY | 1 cut(s) | 336 |
| BssECI | CCNNGG | 1 cut(s) | 395 |
| BssMI | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| BssNI | GRCGYC | 1 cut(s) | 158 |
| Bst2UI | CCWGG | 1 cut(s) | 396 |
| Bst4CI | ACNGT | 5 cut(s) | 169, 202, 445, 463, 574 |
| BstACI | GRCGYC | 1 cut(s) | 158 |
| BstC8I | GCNNGC | 1 cut(s) | 590 |
| BstDEI | CTNAG | 4 cut(s) | 16, 309, 489, 501 |
| BstF5I | GGATG | 3 cut(s) | 248, 795, 930 |
| BstKTI | GATC | 5 cut(s) | 263, 488, 514, 626, 860 |
| BstMAI | GTCTC | 1 cut(s) | 149 |
| BstMBI | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 701, 845 |
| BstNI | CCWGG | 1 cut(s) | 396 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 388, 752 |
| BstSCI | CCNGG | 1 cut(s) | 394 |
| BstSFI | CTRYAG | 1 cut(s) | 909 |
| BstSLI | GKGCMC | 1 cut(s) | 406 |
| BstV1I | GCAGC | 5 cut(s) | 305, 543, 713, 912, 923 |
| BstV2I | GAAGAC | 1 cut(s) | 675 |
| BstX2I | RGATCY | 1 cut(s) | 485 |
| BstYI | RGATCY | 1 cut(s) | 485 |
| Bsu36I | CCTNAGG | 1 cut(s) | 309 |
| BsuRI | GGCC | 2 cut(s) | 336, 588 |
| BtsCI | GGATG | 3 cut(s) | 248, 795, 930 |
| BtsIMutI | CAGTG | 1 cut(s) | 571 |
| BveI | ACCTGC | 1 cut(s) | 87 |
| Cac8I | GCNNGC | 1 cut(s) | 590 |
| Cfr10I | RCCGGY | 1 cut(s) | 336 |
| Cfr13I | GGNCC | 1 cut(s) | 137 |
| CseI | GACGC | 1 cut(s) | 845 |
| Csp6I | GTAC | 2 cut(s) | 100, 270 |
| CviAII | CATG | 4 cut(s) | 229, 410, 419, 679 |
| CviQI | GTAC | 2 cut(s) | 100, 270 |
| DdeI | CTNAG | 4 cut(s) | 16, 309, 489, 501 |
| DpnI | GATC | 5 cut(s) | 262, 487, 513, 625, 859 |
| DpnII | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| EaeI | YGGCCR | 1 cut(s) | 586 |
| EciI | GGCGGA | 1 cut(s) | 802 |
| Eco47I | GGWCC | 1 cut(s) | 137 |
| Eco81I | CCTNAGG | 1 cut(s) | 309 |
| Eco88I | CYCGRG | 1 cut(s) | 368 |
| EcoRI | GAATTC | 1 cut(s) | 636 |
| EcoRII | CCWGG | 1 cut(s) | 394 |
| Esp3I | CGTCTC | 1 cut(s) | 149 |
| FaeI | CATG | 4 cut(s) | 232, 413, 422, 682 |
| FaqI | GGGAC | 1 cut(s) | 289 |
| FatI | CATG | 4 cut(s) | 228, 409, 418, 678 |
| FauNDI | CATATG | 1 cut(s) | 538 |
| FbaI | TGATCA | 1 cut(s) | 511 |
| Fnu4HI | GCNGC | 5 cut(s) | 294, 532, 702, 912, 926 |
| FokI | GGATG | 3 cut(s) | 255, 802, 917 |
| Fsp4HI | GCNGC | 5 cut(s) | 294, 532, 702, 912, 926 |
| FspBI | CTAG | 4 cut(s) | 129, 147, 210, 729 |
| GluI | GCNGC | 5 cut(s) | 294, 532, 702, 912, 926 |
| GsaI | CCCAGC | 1 cut(s) | 635 |
| HaeIII | GGCC | 2 cut(s) | 336, 588 |
| HapII | CCGG | 1 cut(s) | 337 |
| HgaI | GACGC | 1 cut(s) | 845 |
| Hin1I | GRCGYC | 1 cut(s) | 158 |
| Hin1II | CATG | 4 cut(s) | 232, 413, 422, 682 |
| HincII | GTYRAC | 2 cut(s) | 7, 757 |
| HindII | GTYRAC | 2 cut(s) | 7, 757 |
| HinfI | GANTC | 8 cut(s) | 12, 23, 413, 599, 605, 682, 764, 806 |
| HpaII | CCGG | 1 cut(s) | 337 |
| HphI | GGTGA | 2 cut(s) | 187, 384 |
| Hpy166II | GTNNAC | 4 cut(s) | 7, 215, 404, 757 |
| Hpy188I | TCNGA | 5 cut(s) | 163, 525, 604, 805, 823 |
| Hpy188III | TCNNGA | 4 cut(s) | 456, 509, 641, 855 |
| Hpy8I | GTNNAC | 4 cut(s) | 7, 215, 404, 757 |
| HpyAV | CCTTC | 4 cut(s) | 166, 326, 745, 945 |
| HpyCH4III | ACNGT | 5 cut(s) | 169, 202, 445, 463, 574 |
| HpyCH4IV | ACGT | 2 cut(s) | 91, 158 |
| HpyCH4V | TGCA | 3 cut(s) | 404, 689, 911 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 701, 845 |
| HpyF3I | CTNAG | 4 cut(s) | 16, 309, 489, 501 |
| HpySE526I | ACGT | 2 cut(s) | 91, 158 |
| Hsp92I | GRCGYC | 1 cut(s) | 158 |
| Hsp92II | CATG | 4 cut(s) | 232, 413, 422, 682 |
| Ksp22I | TGATCA | 1 cut(s) | 511 |
| Kzo9I | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| LmnI | GCTCC | 1 cut(s) | 369 |
| Lsp1109I | GCAGC | 5 cut(s) | 305, 543, 713, 912, 923 |
| LweI | GCATC | 2 cut(s) | 544, 857 |
| MaeI | CTAG | 4 cut(s) | 129, 147, 210, 729 |
| MaeII | ACGT | 2 cut(s) | 91, 158 |
| MaeIII | GTNAC | 4 cut(s) | 175, 349, 377, 390 |
| MalI | GATC | 5 cut(s) | 262, 487, 513, 625, 859 |
| MboI | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| MfeI | CAATTG | 1 cut(s) | 663 |
| MflI | RGATCY | 1 cut(s) | 485 |
| MhlI | GDGCHC | 1 cut(s) | 406 |
| MlsI | TGGCCA | 1 cut(s) | 588 |
| MluCI | AATT | 3 cut(s) | 636, 663, 705 |
| MluNI | TGGCCA | 1 cut(s) | 588 |
| MlyI | GAGTC | 1 cut(s) | 21 |
| MnlI | CCTC | 6 cut(s) | 143, 318, 621, 621, 685, 708 |
| Mox20I | TGGCCA | 1 cut(s) | 588 |
| MroXI | GAANNNNTTC | 1 cut(s) | 201 |
| MscI | TGGCCA | 1 cut(s) | 588 |
| MslI | CAYNNNNRTG | 1 cut(s) | 423 |
| Msp20I | TGGCCA | 1 cut(s) | 588 |
| MspI | CCGG | 1 cut(s) | 337 |
| MspR9I | CCNGG | 1 cut(s) | 396 |
| MunI | CAATTG | 1 cut(s) | 663 |
| MvaI | CCWGG | 1 cut(s) | 396 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 701, 845 |
| NdeI | CATATG | 1 cut(s) | 538 |
| NdeII | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| NlaIII | CATG | 4 cut(s) | 232, 413, 422, 682 |
| NlaIV | GGNNCC | 1 cut(s) | 365 |
| NmuCI | GTSAC | 2 cut(s) | 175, 390 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 623 |
| PdmI | GAANNNNTTC | 1 cut(s) | 201 |
| PfeI | GAWTC | 7 cut(s) | 23, 413, 599, 605, 682, 764, 806 |
| PflFI | GACNNNGTC | 1 cut(s) | 11 |
| PkrI | GCNGC | 5 cut(s) | 295, 533, 703, 913, 927 |
| PleI | GAGTC | 1 cut(s) | 20 |
| PpsI | GAGTC | 1 cut(s) | 20 |
| PshAI | GACNNNNGTC | 2 cut(s) | 388, 752 |
| Psp6I | CCWGG | 1 cut(s) | 394 |
| PspFI | CCCAGC | 1 cut(s) | 631 |
| PspGI | CCWGG | 1 cut(s) | 394 |
| PspN4I | GGNNCC | 1 cut(s) | 365 |
| PspPI | GGNCC | 1 cut(s) | 137 |
| PstI | CTGCAG | 1 cut(s) | 913 |
| PsuI | RGATCY | 1 cut(s) | 485 |
| PsyI | GACNNNGTC | 1 cut(s) | 11 |
| RsaI | GTAC | 2 cut(s) | 101, 271 |
| RsaNI | GTAC | 2 cut(s) | 100, 270 |
| RseI | CAYNNNNRTG | 1 cut(s) | 423 |
| SatI | GCNGC | 5 cut(s) | 294, 532, 702, 912, 926 |
| Sau3AI | GATC | 5 cut(s) | 260, 485, 511, 623, 857 |
| Sau96I | GGNCC | 1 cut(s) | 137 |
| SchI | GAGTC | 1 cut(s) | 21 |
| ScrFI | CCNGG | 1 cut(s) | 396 |
| SduI | GDGCHC | 1 cut(s) | 406 |
| SfaNI | GCATC | 2 cut(s) | 544, 857 |
| SfcI | CTRYAG | 1 cut(s) | 909 |
| SinI | GGWCC | 1 cut(s) | 137 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 423 |
| SmlI | CTYRAG | 1 cut(s) | 864 |
| SmoI | CTYRAG | 1 cut(s) | 864 |
| Sse9I | AATT | 3 cut(s) | 636, 663, 705 |
| SsiI | CCGC | 2 cut(s) | 38, 787 |
| SspMI | CTAG | 4 cut(s) | 129, 147, 210, 729 |
| StyD4I | CCNGG | 1 cut(s) | 394 |
| TaaI | ACNGT | 5 cut(s) | 169, 202, 445, 463, 574 |
| TaiI | ACGT | 2 cut(s) | 94, 161 |
| TaqI | TCGA | 3 cut(s) | 205, 613, 626 |
| TasI | AATT | 3 cut(s) | 636, 663, 705 |
| TfiI | GAWTC | 7 cut(s) | 23, 413, 599, 605, 682, 764, 806 |
| TscAI | CASTG | 1 cut(s) | 571 |
| TseFI | GTSAC | 2 cut(s) | 175, 390 |
| TseI | GCWGC | 5 cut(s) | 293, 531, 701, 911, 925 |
| Tsp45I | GTSAC | 2 cut(s) | 175, 390 |
| TspGWI | ACGGA | 1 cut(s) | 24 |
| TspRI | CASTG | 1 cut(s) | 571 |
| Tth111I | GACNNNGTC | 1 cut(s) | 11 |
| VneI | GTGCAC | 1 cut(s) | 402 |
| VpaK11BI | GGWCC | 1 cut(s) | 137 |
| XapI | RAATTY | 1 cut(s) | 636 |
| XmnI | GAANNNNTTC | 1 cut(s) | 201 |
| XspI | CTAG | 4 cut(s) | 129, 147, 210, 729 |
| ZraI | GACGTC | 1 cut(s) | 159 |