Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 94, 328 |
| AciI | CCGC | 3 cut(s) | 197, 208, 350 |
| AclWI | GGATC | 1 cut(s) | 545 |
| AfaI | GTAC | 4 cut(s) | 227, 312, 497, 714 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 153, 191, 672 |
| AjnI | CCWGG | 1 cut(s) | 671 |
| AluBI | AGCT | 2 cut(s) | 502, 733 |
| AluI | AGCT | 2 cut(s) | 502, 733 |
| Alw26I | GTCTC | 1 cut(s) | 515 |
| AlwI | GGATC | 1 cut(s) | 545 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 473 |
| AoxI | GGCC | 2 cut(s) | 144, 613 |
| ApeKI | GCWGC | 1 cut(s) | 467 |
| AspS9I | GGNCC | 1 cut(s) | 124 |
| AsuHPI | GGTGA | 2 cut(s) | 73, 661 |
| AvaII | GGWCC | 1 cut(s) | 124 |
| AxyI | CCTNAGG | 1 cut(s) | 617 |
| BbsI | GAAGAC | 1 cut(s) | 505 |
| BbvI | GCAGC | 1 cut(s) | 479 |
| BccI | CCATC | 2 cut(s) | 170, 387 |
| BceAI | ACGGC | 2 cut(s) | 617, 650 |
| BciT130I | CCWGG | 1 cut(s) | 673 |
| BclI | TGATCA | 1 cut(s) | 721 |
| BcoDI | GTCTC | 1 cut(s) | 515 |
| BfaI | CTAG | 1 cut(s) | 86 |
| BisI | GCNGC | 1 cut(s) | 468 |
| BlsI | GCNGC | 1 cut(s) | 469 |
| Bme1390I | CCNGG | 1 cut(s) | 673 |
| Bme18I | GGWCC | 1 cut(s) | 124 |
| BmgT120I | GGNCC | 1 cut(s) | 124 |
| BmiI | GGNNCC | 1 cut(s) | 126 |
| BmrFI | CCNGG | 1 cut(s) | 673 |
| BmsI | GCATC | 3 cut(s) | 190, 454, 699 |
| BpiI | GAAGAC | 1 cut(s) | 505 |
| BsaJI | CCNNGG | 2 cut(s) | 129, 672 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 153, 191, 672 |
| Bse21I | CCTNAGG | 1 cut(s) | 617 |
| Bse3DI | GCAATG | 2 cut(s) | 52, 553 |
| BseBI | CCWGG | 1 cut(s) | 673 |
| BseDI | CCNNGG | 2 cut(s) | 129, 672 |
| BseGI | GGATG | 2 cut(s) | 205, 343 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 153, 191, 672 |
| BseMI | GCAATG | 2 cut(s) | 52, 553 |
| BseMII | CTCAG | 5 cut(s) | 177, 248, 417, 474, 608 |
| BseXI | GCAGC | 1 cut(s) | 479 |
| BshFI | GGCC | 2 cut(s) | 146, 615 |
| BslFI | GGGAC | 1 cut(s) | 599 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 153, 191, 672 |
| BsmAI | GTCTC | 1 cut(s) | 515 |
| BsmFI | GGGAC | 1 cut(s) | 599 |
| BsmI | GAATGC | 1 cut(s) | 310 |
| BsnI | GGCC | 2 cut(s) | 146, 615 |
| Bsp143I | GATC | 2 cut(s) | 550, 721 |
| BspACI | CCGC | 3 cut(s) | 197, 208, 350 |
| BspANI | GGCC | 2 cut(s) | 146, 615 |
| BspCNI | CTCAG | 5 cut(s) | 178, 247, 416, 473, 609 |
| BspLI | GGNNCC | 1 cut(s) | 126 |
| BspPI | GGATC | 1 cut(s) | 545 |
| BsrDI | GCAATG | 2 cut(s) | 52, 553 |
| BssECI | CCNNGG | 2 cut(s) | 129, 672 |
| BssMI | GATC | 2 cut(s) | 550, 721 |
| BssNAI | GTATAC | 1 cut(s) | 329 |
| BssT1I | CCWWGG | 1 cut(s) | 129 |
| Bst1107I | GTATAC | 1 cut(s) | 329 |
| Bst2UI | CCWGG | 1 cut(s) | 673 |
| Bst4CI | ACNGT | 3 cut(s) | 281, 433, 652 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 473, 662 |
| BstDEI | CTNAG | 5 cut(s) | 186, 234, 403, 460, 617 |
| BstEII | GGTNACC | 1 cut(s) | 275 |
| BstF5I | GGATG | 2 cut(s) | 205, 343 |
| BstKTI | GATC | 2 cut(s) | 553, 724 |
| BstMAI | GTCTC | 1 cut(s) | 515 |
| BstMBI | GATC | 2 cut(s) | 550, 721 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 473, 482, 499, 662 |
| BstNI | CCWGG | 1 cut(s) | 673 |
| BstPI | GGTNACC | 1 cut(s) | 275 |
| BstSCI | CCNGG | 1 cut(s) | 671 |
| BstV1I | GCAGC | 1 cut(s) | 479 |
| BstV2I | GAAGAC | 1 cut(s) | 505 |
| BstZ17I | GTATAC | 1 cut(s) | 329 |
| Bsu36I | CCTNAGG | 1 cut(s) | 617 |
| BsuRI | GGCC | 2 cut(s) | 146, 615 |
| BtsCI | GGATG | 2 cut(s) | 205, 343 |
| BtsI | GCAGTG | 1 cut(s) | 228 |
| BtsIMutI | CAGTG | 3 cut(s) | 228, 290, 411 |
| CaiI | CAGNNNCTG | 1 cut(s) | 473 |
| Cfr13I | GGNCC | 1 cut(s) | 124 |
| Csp6I | GTAC | 4 cut(s) | 226, 311, 496, 713 |
| CviAII | CATG | 2 cut(s) | 98, 629 |
| CviJI | RGCY | 6 cut(s) | 39, 146, 419, 502, 615, 733 |
| CviKI_1 | RGCY | 6 cut(s) | 39, 146, 419, 502, 615, 733 |
| CviQI | GTAC | 4 cut(s) | 226, 311, 496, 713 |
| DdeI | CTNAG | 5 cut(s) | 186, 234, 403, 460, 617 |
| DpnI | GATC | 2 cut(s) | 552, 723 |
| DpnII | GATC | 2 cut(s) | 550, 721 |
| EciI | GGCGGA | 1 cut(s) | 339 |
| Eco130I | CCWWGG | 1 cut(s) | 129 |
| Eco32I | GATATC | 1 cut(s) | 259 |
| Eco47I | GGWCC | 1 cut(s) | 124 |
| Eco81I | CCTNAGG | 1 cut(s) | 617 |
| Eco91I | GGTNACC | 1 cut(s) | 275 |
| EcoO65I | GGTNACC | 1 cut(s) | 275 |
| EcoRII | CCWGG | 1 cut(s) | 671 |
| EcoRV | GATATC | 1 cut(s) | 259 |
| EcoT14I | CCWWGG | 1 cut(s) | 129 |
| ErhI | CCWWGG | 1 cut(s) | 129 |
| FaeI | CATG | 2 cut(s) | 101, 632 |
| FaqI | GGGAC | 1 cut(s) | 599 |
| FatI | CATG | 2 cut(s) | 97, 628 |
| FbaI | TGATCA | 1 cut(s) | 721 |
| FblI | GTMKAC | 2 cut(s) | 94, 328 |
| Fnu4HI | GCNGC | 1 cut(s) | 468 |
| FokI | GGATG | 2 cut(s) | 212, 350 |
| Fsp4HI | GCNGC | 1 cut(s) | 468 |
| FspBI | CTAG | 1 cut(s) | 86 |
| GluI | GCNGC | 1 cut(s) | 468 |
| HaeIII | GGCC | 2 cut(s) | 146, 615 |
| Hin1II | CATG | 2 cut(s) | 101, 632 |
| HincII | GTYRAC | 1 cut(s) | 64 |
| HindII | GTYRAC | 1 cut(s) | 64 |
| HinfI | GANTC | 2 cut(s) | 397, 446 |
| HphI | GGTGA | 2 cut(s) | 73, 661 |
| Hpy166II | GTNNAC | 4 cut(s) | 64, 95, 228, 329 |
| Hpy188I | TCNGA | 2 cut(s) | 241, 463 |
| Hpy188III | TCNNGA | 6 cut(s) | 8, 122, 185, 254, 368, 605 |
| Hpy8I | GTNNAC | 4 cut(s) | 64, 95, 228, 329 |
| HpyCH4III | ACNGT | 3 cut(s) | 281, 433, 652 |
| HpyCH4IV | ACGT | 2 cut(s) | 91, 659 |
| HpyCH4V | TGCA | 5 cut(s) | 14, 308, 467, 563, 581 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 473, 482, 499, 662 |
| HpyF3I | CTNAG | 5 cut(s) | 186, 234, 403, 460, 617 |
| HpySE526I | ACGT | 2 cut(s) | 91, 659 |
| Hsp92II | CATG | 2 cut(s) | 101, 632 |
| Ksp22I | TGATCA | 1 cut(s) | 721 |
| Kzo9I | GATC | 2 cut(s) | 550, 721 |
| Lsp1109I | GCAGC | 1 cut(s) | 479 |
| LweI | GCATC | 3 cut(s) | 190, 454, 699 |
| MaeI | CTAG | 1 cut(s) | 86 |
| MaeII | ACGT | 2 cut(s) | 91, 659 |
| MaeIII | GTNAC | 5 cut(s) | 275, 290, 621, 638, 667 |
| MalI | GATC | 2 cut(s) | 552, 723 |
| MboI | GATC | 2 cut(s) | 550, 721 |
| MboII | GAAGA | 2 cut(s) | 505, 657 |
| MluCI | AATT | 3 cut(s) | 134, 158, 697 |
| MnlI | CCTC | 5 cut(s) | 106, 145, 364, 564, 612 |
| MseI | TTAA | 1 cut(s) | 161 |
| MspA1I | CMGCKG | 1 cut(s) | 502 |
| MspR9I | CCNGG | 1 cut(s) | 673 |
| Mva1269I | GAATGC | 1 cut(s) | 310 |
| MvaI | CCWGG | 1 cut(s) | 673 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 473, 482, 499, 662 |
| NdeII | GATC | 2 cut(s) | 550, 721 |
| NlaIII | CATG | 2 cut(s) | 101, 632 |
| NlaIV | GGNNCC | 1 cut(s) | 126 |
| NmuCI | GTSAC | 2 cut(s) | 638, 667 |
| PctI | GAATGC | 1 cut(s) | 310 |
| PfeI | GAWTC | 2 cut(s) | 397, 446 |
| PkrI | GCNGC | 1 cut(s) | 469 |
| Psp6I | CCWGG | 1 cut(s) | 671 |
| PspEI | GGTNACC | 1 cut(s) | 275 |
| PspGI | CCWGG | 1 cut(s) | 671 |
| PspN4I | GGNNCC | 1 cut(s) | 126 |
| PspPI | GGNCC | 1 cut(s) | 124 |
| PstNI | CAGNNNCTG | 1 cut(s) | 473 |
| PvuII | CAGCTG | 1 cut(s) | 502 |
| RsaI | GTAC | 4 cut(s) | 227, 312, 497, 714 |
| RsaNI | GTAC | 4 cut(s) | 226, 311, 496, 713 |
| SaqAI | TTAA | 1 cut(s) | 161 |
| SatI | GCNGC | 1 cut(s) | 468 |
| Sau3AI | GATC | 2 cut(s) | 550, 721 |
| Sau96I | GGNCC | 1 cut(s) | 124 |
| ScrFI | CCNGG | 1 cut(s) | 673 |
| SetI | ASST | 6 cut(s) | 94, 504, 535, 623, 662, 735 |
| SfaNI | GCATC | 3 cut(s) | 190, 454, 699 |
| SinI | GGWCC | 1 cut(s) | 124 |
| Sse9I | AATT | 3 cut(s) | 134, 158, 697 |
| SsiI | CCGC | 3 cut(s) | 197, 208, 350 |
| SspMI | CTAG | 1 cut(s) | 86 |
| StyD4I | CCNGG | 1 cut(s) | 671 |
| StyI | CCWWGG | 1 cut(s) | 129 |
| TaaI | ACNGT | 3 cut(s) | 281, 433, 652 |
| TaiI | ACGT | 2 cut(s) | 94, 662 |
| TaqI | TCGA | 1 cut(s) | 7 |
| TasI | AATT | 3 cut(s) | 134, 158, 697 |
| TfiI | GAWTC | 2 cut(s) | 397, 446 |
| Tru1I | TTAA | 1 cut(s) | 161 |
| Tru9I | TTAA | 1 cut(s) | 161 |
| TscAI | CASTG | 3 cut(s) | 235, 290, 411 |
| TseFI | GTSAC | 2 cut(s) | 638, 667 |
| TseI | GCWGC | 1 cut(s) | 467 |
| Tsp45I | GTSAC | 2 cut(s) | 638, 667 |
| TspDTI | ATGAA | 3 cut(s) | 429, 505, 617 |
| TspRI | CASTG | 3 cut(s) | 235, 290, 411 |
| VpaK11BI | GGWCC | 1 cut(s) | 124 |
| XmiI | GTMKAC | 2 cut(s) | 94, 328 |
| XspI | CTAG | 1 cut(s) | 86 |