Belongs to the class-I aminoacyl-tRNA synthetase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| tRNA-synt_1 | PF00133 | 145 - 766 | 4.4e-143 | tRNA synthetases class I (I, L, M and V) |
| tRNA-synt_1g | PF09334 | 171 - 233 | 6.9e-07 | tRNA synthetases class I (M) |
| tRNA-synt_1_2 | PF13603 | 387 - 447 | 5.7e-07 | Leucyl-tRNA synthetase, editing domain |
| Anticodon_1 | PF08264 | 812 - 952 | 4.7e-33 | Anticodon-binding domain of tRNA ligase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1319 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1289 |
| AccB1I | GGYRCC | 1 cut(s) | 619 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 1187, 1238, 3259 |
| AccI | GTMKAC | 1 cut(s) | 2926 |
| AccII | CGCG | 1 cut(s) | 533 |
| AccIII | TCCGGA | 1 cut(s) | 3093 |
| AciI | CCGC | 7 cut(s) | 35, 112, 512, 904, 1681, 2393, 2710 |
| AclWI | GGATC | 8 cut(s) | 344, 1223, 1268, 1741, 1826, 1839, 2093, 3085 |
| AcoI | YGGCCR | 5 cut(s) | 138, 367, 396, 1539, 1892 |
| AcsI | RAATTY | 3 cut(s) | 719, 1019, 2600 |
| AcuI | CTGAAG | 4 cut(s) | 750, 1428, 1917, 3068 |
| AcvI | CACGTG | 1 cut(s) | 1851 |
| AcyI | GRCGYC | 1 cut(s) | 2875 |
| AfaI | GTAC | 9 cut(s) | 407, 461, 1004, 1128, 1999, 2016, 2355, 2451, 2540 |
| AflIII | ACRYGT | 1 cut(s) | 1848 |
| AjnI | CCWGG | 4 cut(s) | 255, 622, 997, 1350 |
| AleI | CACNNNNGTG | 3 cut(s) | 1091, 2027, 2519 |
| Alw21I | GWGCWC | 1 cut(s) | 485 |
| Alw26I | GTCTC | 8 cut(s) | 1594, 1660, 1871, 2269, 2859, 2909, 3066, 3240 |
| AlwI | GGATC | 8 cut(s) | 344, 1223, 1268, 1741, 1826, 1839, 2093, 3085 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1406, 2516 |
| Ama87I | CYCGRG | 3 cut(s) | 1392, 3176, 3218 |
| Aor13HI | TCCGGA | 1 cut(s) | 3093 |
| ApaI | GGGCCC | 2 cut(s) | 12, 1552 |
| ApoI | RAATTY | 3 cut(s) | 719, 1019, 2600 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3156, 3188 |
| AspLEI | GCGC | 6 cut(s) | 85, 533, 535, 1302, 2248, 3268 |
| AsuC2I | CCSGG | 3 cut(s) | 173, 1393, 1394 |
| AsuHPI | GGTGA | 6 cut(s) | 1079, 1121, 1670, 1724, 2663, 3154 |
| AsuII | TTCGAA | 1 cut(s) | 281 |
| AvaI | CYCGRG | 3 cut(s) | 1392, 3176, 3218 |
| AvaII | GGWCC | 4 cut(s) | 631, 807, 2167, 3172 |
| BaeGI | GKGCMC | 2 cut(s) | 12, 1552 |
| BalI | TGGCCA | 3 cut(s) | 369, 398, 1541 |
| BamHI | GGATCC | 1 cut(s) | 1831 |
| BanI | GGYRCC | 1 cut(s) | 619 |
| BanII | GRGCYC | 3 cut(s) | 12, 485, 1552 |
| BbrPI | CACGTG | 1 cut(s) | 1851 |
| BbsI | GAAGAC | 4 cut(s) | 174, 1062, 1318, 3114 |
| Bbv12I | GWGCWC | 1 cut(s) | 485 |
| BceAI | ACGGC | 3 cut(s) | 951, 1676, 2678 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 764, 798 |
| BciT130I | CCWGG | 4 cut(s) | 257, 624, 999, 1352 |
| BciVI | GTATCC | 2 cut(s) | 2433, 2644 |
| BclI | TGATCA | 2 cut(s) | 2112, 3144 |
| BcnI | CCSGG | 3 cut(s) | 173, 1393, 1394 |
| BcoDI | GTCTC | 8 cut(s) | 1594, 1660, 1871, 2269, 2859, 2909, 3066, 3240 |
| BfaI | CTAG | 4 cut(s) | 908, 1476, 1823, 2936 |
| BfmI | CTRYAG | 6 cut(s) | 770, 892, 1563, 2184, 2517, 3069 |
| BfoI | RGCGCY | 2 cut(s) | 86, 1303 |
| BfuI | GTATCC | 2 cut(s) | 2433, 2644 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1397, 1547 |
| BglII | AGATCT | 3 cut(s) | 2134, 2837, 3079 |
| Bme1390I | CCNGG | 7 cut(s) | 173, 257, 624, 999, 1352, 1393, 1394 |
| Bme18I | GGWCC | 4 cut(s) | 631, 807, 2167, 3172 |
| BmeT110I | CYCGRG | 3 cut(s) | 1392, 3176, 3218 |
| BmrFI | CCNGG | 7 cut(s) | 173, 257, 624, 999, 1352, 1393, 1394 |
| BmrI | ACTGGG | 2 cut(s) | 1507, 1956 |
| BmuI | ACTGGG | 2 cut(s) | 1507, 1956 |
| BpiI | GAAGAC | 4 cut(s) | 174, 1062, 1318, 3114 |
| Bpu14I | TTCGAA | 1 cut(s) | 281 |
| BpuEI | CTTGAG | 4 cut(s) | 1010, 1331, 1652, 2841 |
| BpuMI | CCSGG | 3 cut(s) | 173, 1393, 1394 |
| BsaAI | YACGTR | 1 cut(s) | 1851 |
| BsaHI | GRCGYC | 1 cut(s) | 2875 |
| BsaI | GGTCTC | 3 cut(s) | 1871, 2269, 3066 |
| BsaJI | CCNNGG | 6 cut(s) | 150, 998, 1392, 1542, 2082, 2171 |
| BsaWI | WCCGGW | 3 cut(s) | 361, 2844, 3093 |
| Bse118I | RCCGGY | 3 cut(s) | 140, 2322, 2433 |
| Bse1I | ACTGG | 8 cut(s) | 532, 1249, 1513, 1791, 1951, 2133, 2659, 3007 |
| BseAI | TCCGGA | 1 cut(s) | 3093 |
| BseBI | CCWGG | 4 cut(s) | 257, 624, 999, 1352 |
| BseDI | CCNNGG | 6 cut(s) | 150, 998, 1392, 1542, 2082, 2171 |
| BseMII | CTCAG | 2 cut(s) | 665, 2291 |
| BseNI | ACTGG | 8 cut(s) | 532, 1249, 1513, 1791, 1951, 2133, 2659, 3007 |
| BsePI | GCGCGC | 1 cut(s) | 531 |
| BseRI | GAGGAG | 2 cut(s) | 83, 108 |
| BseSI | GKGCMC | 2 cut(s) | 12, 1552 |
| BseX3I | CGGCCG | 1 cut(s) | 138 |
| BseYI | CCCAGC | 2 cut(s) | 638, 2878 |
| Bsh1236I | CGCG | 1 cut(s) | 533 |
| Bsh1285I | CGRYCG | 1 cut(s) | 141 |
| BshNI | GGYRCC | 1 cut(s) | 619 |
| BsiEI | CGRYCG | 1 cut(s) | 141 |
| BsiHKAI | GWGCWC | 1 cut(s) | 485 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1392, 3176, 3218 |
| BslFI | GGGAC | 3 cut(s) | 793, 1255, 3158 |
| BsmAI | GTCTC | 8 cut(s) | 1594, 1660, 1871, 2269, 2859, 2909, 3066, 3240 |
| BsmBI | CGTCTC | 1 cut(s) | 1660 |
| BsmFI | GGGAC | 3 cut(s) | 793, 1255, 3158 |
| BsmI | GAATGC | 3 cut(s) | 277, 1683, 2353 |
| Bso31I | GGTCTC | 3 cut(s) | 1871, 2269, 3066 |
| BsoBI | CYCGRG | 3 cut(s) | 1392, 3176, 3218 |
| Bsp119I | TTCGAA | 1 cut(s) | 281 |
| Bsp120I | GGGCCC | 2 cut(s) | 8, 1548 |
| Bsp1286I | GDGCHC | 3 cut(s) | 12, 485, 1552 |
| Bsp13I | TCCGGA | 1 cut(s) | 3093 |
| Bsp1407I | TGTACA | 1 cut(s) | 1126 |
| Bsp19I | CCATGG | 1 cut(s) | 150 |
| BspACI | CCGC | 7 cut(s) | 35, 112, 512, 904, 1681, 2393, 2710 |
| BspCNI | CTCAG | 2 cut(s) | 664, 2290 |
| BspEI | TCCGGA | 1 cut(s) | 3093 |
| BspFNI | CGCG | 1 cut(s) | 533 |
| BspMAI | CTGCAG | 2 cut(s) | 2521, 3073 |
| BspPI | GGATC | 8 cut(s) | 344, 1223, 1268, 1741, 1826, 1839, 2093, 3085 |
| BspT104I | TTCGAA | 1 cut(s) | 281 |
| BspT107I | GGYRCC | 1 cut(s) | 619 |
| BspTNI | GGTCTC | 3 cut(s) | 1871, 2269, 3066 |
| BsrFI | RCCGGY | 3 cut(s) | 140, 2322, 2433 |
| BsrGI | TGTACA | 1 cut(s) | 1126 |
| BsrI | ACTGG | 8 cut(s) | 532, 1249, 1513, 1791, 1951, 2133, 2659, 3007 |
| BssAI | RCCGGY | 3 cut(s) | 140, 2322, 2433 |
| BssECI | CCNNGG | 6 cut(s) | 150, 998, 1392, 1542, 2082, 2171 |
| BssHII | GCGCGC | 1 cut(s) | 531 |
| BssNI | GRCGYC | 1 cut(s) | 2875 |
| BssT1I | CCWWGG | 4 cut(s) | 150, 1542, 2082, 2171 |
| Bst2UI | CCWGG | 4 cut(s) | 257, 624, 999, 1352 |
| Bst4CI | ACNGT | 9 cut(s) | 405, 410, 758, 1013, 1605, 2404, 2853, 2974, 3234 |
| BstACI | GRCGYC | 1 cut(s) | 2875 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1697, 2516, 2525 |
| BstAUI | TGTACA | 1 cut(s) | 1126 |
| BstBAI | YACGTR | 1 cut(s) | 1851 |
| BstBI | TTCGAA | 1 cut(s) | 281 |
| BstC8I | GCNNGC | 4 cut(s) | 142, 533, 1170, 3250 |
| BstDEI | CTNAG | 4 cut(s) | 387, 477, 651, 2277 |
| BstDSI | CCRYGG | 1 cut(s) | 150 |
| BstEII | GGTNACC | 2 cut(s) | 1676, 2318 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 16, 3221 |
| BstFNI | CGCG | 1 cut(s) | 533 |
| BstH2I | RGCGCY | 2 cut(s) | 86, 1303 |
| BstHHI | GCGC | 6 cut(s) | 85, 533, 535, 1302, 2248, 3268 |
| BstMAI | GTCTC | 8 cut(s) | 1594, 1660, 1871, 2269, 2859, 2909, 3066, 3240 |
| BstMCI | CGRYCG | 1 cut(s) | 141 |
| BstNI | CCWGG | 4 cut(s) | 257, 624, 999, 1352 |
| BstNSI | RCATGY | 1 cut(s) | 1172 |
| BstPI | GGTNACC | 2 cut(s) | 1676, 2318 |
| BstSCI | CCNGG | 7 cut(s) | 171, 255, 622, 997, 1350, 1391, 1392 |
| BstSFI | CTRYAG | 6 cut(s) | 770, 892, 1563, 2184, 2517, 3069 |
| BstSLI | GKGCMC | 2 cut(s) | 12, 1552 |
| BstUI | CGCG | 1 cut(s) | 533 |
| BstV2I | GAAGAC | 4 cut(s) | 174, 1062, 1318, 3114 |
| BstX2I | RGATCY | 5 cut(s) | 1831, 2134, 2837, 3079, 3090 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1093, 1358, 2083 |
| BstYI | RGATCY | 5 cut(s) | 1831, 2134, 2837, 3079, 3090 |
| BstZI | CGGCCG | 1 cut(s) | 138 |
| BsuI | GTATCC | 2 cut(s) | 2433, 2644 |
| BtgI | CCRYGG | 1 cut(s) | 150 |
| BtsI | GCAGTG | 4 cut(s) | 2271, 2333, 2514, 2526 |
| Cac8I | GCNNGC | 4 cut(s) | 142, 533, 1170, 3250 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1406, 2516 |
| CfoI | GCGC | 6 cut(s) | 85, 533, 535, 1302, 2248, 3268 |
| Cfr10I | RCCGGY | 3 cut(s) | 140, 2322, 2433 |
| Cfr9I | CCCGGG | 1 cut(s) | 1392 |
| CseI | GACGC | 4 cut(s) | 164, 1448, 1642, 2883 |
| Csp6I | GTAC | 9 cut(s) | 406, 460, 1003, 1127, 1998, 2015, 2354, 2450, 2539 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1513, 1548, 1652, 1687 |
| CviQI | GTAC | 9 cut(s) | 406, 460, 1003, 1127, 1998, 2015, 2354, 2450, 2539 |
| DdeI | CTNAG | 4 cut(s) | 387, 477, 651, 2277 |
| DraI | TTTAAA | 1 cut(s) | 1435 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1289 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1289 |
| EaeI | YGGCCR | 5 cut(s) | 138, 367, 396, 1539, 1892 |
| EagI | CGGCCG | 1 cut(s) | 138 |
| EciI | GGCGGA | 2 cut(s) | 101, 2382 |
| Ecl136II | GAGCTC | 1 cut(s) | 483 |
| EclXI | CGGCCG | 1 cut(s) | 138 |
| Eco130I | CCWWGG | 4 cut(s) | 150, 1542, 2082, 2171 |
| Eco24I | GRGCYC | 3 cut(s) | 12, 485, 1552 |
| Eco31I | GGTCTC | 3 cut(s) | 1871, 2269, 3066 |
| Eco32I | GATATC | 2 cut(s) | 2302, 3273 |
| Eco47I | GGWCC | 4 cut(s) | 631, 807, 2167, 3172 |
| Eco52I | CGGCCG | 1 cut(s) | 138 |
| Eco53kI | GAGCTC | 1 cut(s) | 483 |
| Eco57I | CTGAAG | 4 cut(s) | 750, 1428, 1917, 3068 |
| Eco72I | CACGTG | 1 cut(s) | 1851 |
| Eco88I | CYCGRG | 3 cut(s) | 1392, 3176, 3218 |
| Eco91I | GGTNACC | 2 cut(s) | 1676, 2318 |
| EcoICRI | GAGCTC | 1 cut(s) | 483 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 16, 3221 |
| EcoO109I | RGGNCCY | 5 cut(s) | 9, 1426, 1548, 1549, 3172 |
| EcoO65I | GGTNACC | 2 cut(s) | 1676, 2318 |
| EcoRII | CCWGG | 4 cut(s) | 255, 622, 997, 1350 |
| EcoRV | GATATC | 2 cut(s) | 2302, 3273 |
| EcoT14I | CCWWGG | 4 cut(s) | 150, 1542, 2082, 2171 |
| EcoT38I | GRGCYC | 3 cut(s) | 12, 485, 1552 |
| ErhI | CCWWGG | 4 cut(s) | 150, 1542, 2082, 2171 |
| Esp3I | CGTCTC | 1 cut(s) | 1660 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1415, 1447 |
| FaqI | GGGAC | 3 cut(s) | 793, 1255, 3158 |
| FauNDI | CATATG | 2 cut(s) | 2494, 3258 |
| FbaI | TGATCA | 2 cut(s) | 2112, 3144 |
| FblI | GTMKAC | 1 cut(s) | 2926 |
| FriOI | GRGCYC | 3 cut(s) | 12, 485, 1552 |
| FspBI | CTAG | 4 cut(s) | 908, 1476, 1823, 2936 |
| GlaI | GCGC | 6 cut(s) | 84, 532, 534, 1301, 2247, 3267 |
| GsaI | CCCAGC | 2 cut(s) | 642, 2882 |
| HaeII | RGCGCY | 2 cut(s) | 86, 1303 |
| HgaI | GACGC | 4 cut(s) | 164, 1448, 1642, 2883 |
| HhaI | GCGC | 6 cut(s) | 85, 533, 535, 1302, 2248, 3268 |
| Hin1I | GRCGYC | 1 cut(s) | 2875 |
| Hin6I | GCGC | 6 cut(s) | 83, 531, 533, 1300, 2246, 3266 |
| HinP1I | GCGC | 6 cut(s) | 83, 531, 533, 1300, 2246, 3266 |
| HindIII | AAGCTT | 2 cut(s) | 1583, 2147 |
| HphI | GGTGA | 6 cut(s) | 1079, 1121, 1670, 1724, 2663, 3154 |
| Hpy166II | GTNNAC | 4 cut(s) | 683, 958, 2927, 3025 |
| Hpy8I | GTNNAC | 4 cut(s) | 683, 958, 2927, 3025 |
| Hpy99I | CGWCG | 1 cut(s) | 786 |
| HpyAV | CCTTC | 8 cut(s) | 184, 193, 1188, 1417, 2189, 3043, 3133, 3217 |
| HpyCH4III | ACNGT | 9 cut(s) | 405, 410, 758, 1013, 1605, 2404, 2853, 2974, 3234 |
| HpyCH4IV | ACGT | 3 cut(s) | 781, 1323, 1850 |
| HpyF3I | CTNAG | 4 cut(s) | 387, 477, 651, 2277 |
| HpySE526I | ACGT | 3 cut(s) | 781, 1323, 1850 |
| Hsp92I | GRCGYC | 1 cut(s) | 2875 |
| HspAI | GCGC | 6 cut(s) | 83, 531, 533, 1300, 2246, 3266 |
| Kpn2I | TCCGGA | 1 cut(s) | 3093 |
| KroI | GCCGGC | 1 cut(s) | 140 |
| KroNI | GCCGGC | 1 cut(s) | 142 |
| Ksp22I | TGATCA | 2 cut(s) | 2112, 3144 |
| LmnI | GCTCC | 2 cut(s) | 139, 2718 |
| MaeI | CTAG | 4 cut(s) | 908, 1476, 1823, 2936 |
| MaeII | ACGT | 3 cut(s) | 781, 1323, 1850 |
| MfeI | CAATTG | 1 cut(s) | 2543 |
| MflI | RGATCY | 5 cut(s) | 1831, 2134, 2837, 3079, 3090 |
| MhlI | GDGCHC | 3 cut(s) | 12, 485, 1552 |
| MlsI | TGGCCA | 3 cut(s) | 369, 398, 1541 |
| MluNI | TGGCCA | 3 cut(s) | 369, 398, 1541 |
| MlyI | GAGTC | 5 cut(s) | 352, 2813, 2819, 2824, 2872 |
| MmeI | TCCRAC | 3 cut(s) | 380, 1273, 1952 |
| Mox20I | TGGCCA | 3 cut(s) | 369, 398, 1541 |
| MroI | TCCGGA | 1 cut(s) | 3093 |
| MroNI | GCCGGC | 1 cut(s) | 140 |
| MscI | TGGCCA | 3 cut(s) | 369, 398, 1541 |
| MseI | TTAA | 8 cut(s) | 692, 1031, 1185, 1415, 1434, 1818, 1913, 3114 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1091, 1134, 1158, 2027, 2519 |
| Msp20I | TGGCCA | 3 cut(s) | 369, 398, 1541 |
| MspA1I | CMGCKG | 2 cut(s) | 338, 2710 |
| MspR9I | CCNGG | 7 cut(s) | 173, 257, 624, 999, 1352, 1393, 1394 |
| MunI | CAATTG | 1 cut(s) | 2543 |
| Mva1269I | GAATGC | 3 cut(s) | 277, 1683, 2353 |
| MvaI | CCWGG | 4 cut(s) | 257, 624, 999, 1352 |
| MvnI | CGCG | 1 cut(s) | 533 |
| NaeI | GCCGGC | 1 cut(s) | 142 |
| NciI | CCSGG | 3 cut(s) | 173, 1393, 1394 |
| NcoI | CCATGG | 1 cut(s) | 150 |
| NdeI | CATATG | 2 cut(s) | 2494, 3258 |
| NgoMIV | GCCGGC | 1 cut(s) | 140 |
| NmuCI | GTSAC | 7 cut(s) | 98, 523, 713, 850, 1676, 1712, 2847 |
| NspI | RCATGY | 1 cut(s) | 1172 |
| NspV | TTCGAA | 1 cut(s) | 281 |
| OliI | CACNNNNGTG | 3 cut(s) | 1091, 2027, 2519 |
| PaeI | GCATGC | 1 cut(s) | 1172 |
| PaeR7I | CTCGAG | 1 cut(s) | 3218 |
| PauI | GCGCGC | 1 cut(s) | 531 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 162 |
| PctI | GAATGC | 3 cut(s) | 277, 1683, 2353 |
| PdiI | GCCGGC | 1 cut(s) | 142 |
| PfeI | GAWTC | 7 cut(s) | 162, 580, 1198, 2047, 2489, 3184, 3212 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 1187, 1238, 3259 |
| PleI | GAGTC | 5 cut(s) | 352, 2812, 2819, 2823, 2871 |
| PmaCI | CACGTG | 1 cut(s) | 1851 |
| PmlI | CACGTG | 1 cut(s) | 1851 |
| PpsI | GAGTC | 5 cut(s) | 352, 2812, 2819, 2823, 2871 |
| Ppu21I | YACGTR | 1 cut(s) | 1851 |
| PpuMI | RGGWCCY | 1 cut(s) | 3172 |
| PsiI | TTATAA | 1 cut(s) | 1319 |
| Psp124BI | GAGCTC | 1 cut(s) | 485 |
| Psp5II | RGGWCCY | 1 cut(s) | 3172 |
| Psp6I | CCWGG | 4 cut(s) | 255, 622, 997, 1350 |
| PspCI | CACGTG | 1 cut(s) | 1851 |
| PspEI | GGTNACC | 2 cut(s) | 1676, 2318 |
| PspFI | CCCAGC | 2 cut(s) | 638, 2878 |
| PspGI | CCWGG | 4 cut(s) | 255, 622, 997, 1350 |
| PspOMI | GGGCCC | 2 cut(s) | 8, 1548 |
| PspPPI | RGGWCCY | 1 cut(s) | 3172 |
| PstI | CTGCAG | 2 cut(s) | 2521, 3073 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1406, 2516 |
| PsuI | RGATCY | 5 cut(s) | 1831, 2134, 2837, 3079, 3090 |
| PteI | GCGCGC | 1 cut(s) | 531 |
| PvuII | CAGCTG | 1 cut(s) | 338 |
| RsaI | GTAC | 9 cut(s) | 407, 461, 1004, 1128, 1999, 2016, 2355, 2451, 2540 |
| RsaNI | GTAC | 9 cut(s) | 406, 460, 1003, 1127, 1998, 2015, 2354, 2450, 2539 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1091, 1134, 1158, 2027, 2519 |
| SacI | GAGCTC | 1 cut(s) | 485 |
| SaqAI | TTAA | 8 cut(s) | 692, 1031, 1185, 1415, 1434, 1818, 1913, 3114 |
| SchI | GAGTC | 5 cut(s) | 352, 2813, 2819, 2824, 2872 |
| ScrFI | CCNGG | 7 cut(s) | 173, 257, 624, 999, 1352, 1393, 1394 |
| SduI | GDGCHC | 3 cut(s) | 12, 485, 1552 |
| SfcI | CTRYAG | 6 cut(s) | 770, 892, 1563, 2184, 2517, 3069 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 1547 |
| Sfr274I | CTCGAG | 1 cut(s) | 3218 |
| SfuI | TTCGAA | 1 cut(s) | 281 |
| SinI | GGWCC | 4 cut(s) | 631, 807, 2167, 3172 |
| SlaI | CTCGAG | 1 cut(s) | 3218 |
| SmaI | CCCGGG | 1 cut(s) | 1394 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1091, 1134, 1158, 2027, 2519 |
| SmlI | CTYRAG | 5 cut(s) | 989, 1310, 1631, 2856, 3218 |
| SmoI | CTYRAG | 5 cut(s) | 989, 1310, 1631, 2856, 3218 |
| SphI | GCATGC | 1 cut(s) | 1172 |
| SsiI | CCGC | 7 cut(s) | 35, 112, 512, 904, 1681, 2393, 2710 |
| SspI | AATATT | 1 cut(s) | 1639 |
| SspMI | CTAG | 4 cut(s) | 908, 1476, 1823, 2936 |
| SstI | GAGCTC | 1 cut(s) | 485 |
| StyD4I | CCNGG | 7 cut(s) | 171, 255, 622, 997, 1350, 1391, 1392 |
| StyI | CCWWGG | 4 cut(s) | 150, 1542, 2082, 2171 |
| TaaI | ACNGT | 9 cut(s) | 405, 410, 758, 1013, 1605, 2404, 2853, 2974, 3234 |
| TaiI | ACGT | 3 cut(s) | 784, 1326, 1853 |
| TaqI | TCGA | 6 cut(s) | 165, 281, 423, 2896, 3219, 3240 |
| TaqII | GACCGA | 1 cut(s) | 1761 |
| TatI | WGTACW | 3 cut(s) | 405, 1126, 1997 |
| TauI | GCSGC | 3 cut(s) | 37, 514, 2713 |
| TfiI | GAWTC | 7 cut(s) | 162, 580, 1198, 2047, 2489, 3184, 3212 |
| Tru1I | TTAA | 8 cut(s) | 692, 1031, 1185, 1415, 1434, 1818, 1913, 3114 |
| Tru9I | TTAA | 8 cut(s) | 692, 1031, 1185, 1415, 1434, 1818, 1913, 3114 |
| TseFI | GTSAC | 7 cut(s) | 98, 523, 713, 850, 1676, 1712, 2847 |
| Tsp45I | GTSAC | 7 cut(s) | 98, 523, 713, 850, 1676, 1712, 2847 |
| TspDTI | ATGAA | 9 cut(s) | 333, 342, 527, 786, 863, 1148, 1166, 2548, 2808 |
| TspGWI | ACGGA | 4 cut(s) | 86, 483, 869, 1870 |
| TspMI | CCCGGG | 1 cut(s) | 1392 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 1187, 1238, 3259 |
| VpaK11BI | GGWCC | 4 cut(s) | 631, 807, 2167, 3172 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 16, 3221 |
| XapI | RAATTY | 3 cut(s) | 719, 1019, 2600 |
| XbaI | TCTAGA | 1 cut(s) | 1475 |
| XceI | RCATGY | 1 cut(s) | 1172 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2312 |
| XhoI | CTCGAG | 1 cut(s) | 3218 |
| XmaI | CCCGGG | 1 cut(s) | 1392 |
| XmiI | GTMKAC | 1 cut(s) | 2926 |
| XspI | CTAG | 4 cut(s) | 908, 1476, 1823, 2936 |