Belongs to the class-I aminoacyl-tRNA synthetase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| tRNA-synt_1 | PF00133 | 107 - 483 | 1.7e-65 | tRNA synthetases class I (I, L, M and V) |
| tRNA-synt_1_2 | PF13603 | 347 - 407 | 3.6e-06 | Leucyl-tRNA synthetase, editing domain |
| tRNA-synt_1 | PF00133 | 487 - 706 | 5.3e-42 | tRNA synthetases class I (I, L, M and V) |
| Anticodon_1 | PF08264 | 752 - 884 | 4.4e-29 | Anticodon-binding domain of tRNA ligase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 759, 1269, 2409 |
| AccB1I | GGYRCC | 1 cut(s) | 644 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1118 |
| AccI | GTMKAC | 1 cut(s) | 1619 |
| AccIII | TCCGGA | 1 cut(s) | 1012 |
| AciI | CCGC | 5 cut(s) | 117, 395, 472, 475, 2057 |
| AclWI | GGATC | 4 cut(s) | 1103, 1148, 1910, 2408 |
| AcoI | YGGCCR | 1 cut(s) | 1688 |
| AcsI | RAATTY | 3 cut(s) | 1115, 1247, 1583 |
| AcuI | CTGAAG | 4 cut(s) | 113, 893, 1311, 1968 |
| AfaI | GTAC | 4 cut(s) | 954, 1816, 2271, 2360 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 750, 1067, 1118, 2013 |
| AgsI | TTSAA | 6 cut(s) | 275, 857, 1517, 1739, 1971, 2384 |
| AjnI | CCWGG | 3 cut(s) | 505, 578, 880 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 969, 1001 |
| AleI | CACNNNNGTG | 1 cut(s) | 413 |
| Alw21I | GWGCWC | 1 cut(s) | 87 |
| Alw26I | GTCTC | 3 cut(s) | 273, 674, 1272 |
| AlwI | GGATC | 4 cut(s) | 1103, 1148, 1910, 2408 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 569, 1574 |
| Ama87I | CYCGRG | 1 cut(s) | 1143 |
| Aor13HI | TCCGGA | 1 cut(s) | 1012 |
| AoxI | GGCC | 4 cut(s) | 427, 907, 1688, 2464 |
| ApeKI | GCWGC | 7 cut(s) | 655, 658, 1231, 1574, 1831, 2330, 2534 |
| ApoI | RAATTY | 3 cut(s) | 1115, 1247, 1583 |
| AseI | ATTAAT | 1 cut(s) | 2226 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2027 |
| AspLEI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| AspS9I | GGNCC | 2 cut(s) | 514, 1627 |
| AsuC2I | CCSGG | 3 cut(s) | 8, 1144, 1145 |
| AsuHPI | GGTGA | 5 cut(s) | 221, 1001, 1297, 1454, 1817 |
| AsuII | TTCGAA | 1 cut(s) | 1167 |
| AvaI | CYCGRG | 1 cut(s) | 1143 |
| AvaII | GGWCC | 2 cut(s) | 514, 1627 |
| AxyI | CCTNAGG | 2 cut(s) | 1506, 2468 |
| BalI | TGGCCA | 1 cut(s) | 1690 |
| BanI | GGYRCC | 1 cut(s) | 644 |
| BanII | GRGCYC | 2 cut(s) | 87, 1383 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 936, 968 |
| BbsI | GAAGAC | 3 cut(s) | 1710, 1719, 2513 |
| Bbv12I | GWGCWC | 1 cut(s) | 87 |
| BbvI | GCAGC | 7 cut(s) | 667, 670, 1243, 1561, 1818, 2342, 2546 |
| BccI | CCATC | 7 cut(s) | 200, 1211, 1527, 1805, 2548, 2575, 2605 |
| BceAI | ACGGC | 1 cut(s) | 651 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 2130, 2164, 2526, 2560 |
| BciT130I | CCWGG | 3 cut(s) | 507, 580, 882 |
| BciVI | GTATCC | 2 cut(s) | 402, 2253 |
| BcnI | CCSGG | 3 cut(s) | 8, 1144, 1145 |
| BcoDI | GTCTC | 3 cut(s) | 273, 674, 1272 |
| BfaI | CTAG | 4 cut(s) | 425, 1359, 1563, 2654 |
| BfmI | CTRYAG | 2 cut(s) | 147, 775 |
| BfoI | RGCGCY | 1 cut(s) | 1183 |
| BfuI | GTATCC | 2 cut(s) | 402, 2253 |
| BisI | GCNGC | 9 cut(s) | 117, 473, 656, 659, 1232, 1575, 1832, 2331, 2535 |
| BlsI | GCNGC | 9 cut(s) | 118, 474, 657, 660, 1233, 1576, 1833, 2332, 2536 |
| BmcAI | AGTACT | 1 cut(s) | 2271 |
| Bme1390I | CCNGG | 6 cut(s) | 8, 507, 580, 882, 1144, 1145 |
| Bme18I | GGWCC | 2 cut(s) | 514, 1627 |
| BmeT110I | CYCGRG | 1 cut(s) | 1143 |
| BmgT120I | GGNCC | 2 cut(s) | 514, 1627 |
| BmiI | GGNNCC | 4 cut(s) | 646, 879, 1380, 1629 |
| BmrFI | CCNGG | 6 cut(s) | 8, 507, 580, 882, 1144, 1145 |
| BmrI | ACTGGG | 2 cut(s) | 579, 1752 |
| BmsI | GCATC | 8 cut(s) | 346, 1018, 1042, 1084, 1248, 1861, 2050, 2317 |
| BmuI | ACTGGG | 2 cut(s) | 579, 1752 |
| BpiI | GAAGAC | 3 cut(s) | 1710, 1719, 2513 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 707, 739, 827, 859 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2334 |
| Bpu14I | TTCGAA | 1 cut(s) | 1167 |
| BpuEI | CTTGAG | 2 cut(s) | 1211, 1283 |
| BpuMI | CCSGG | 3 cut(s) | 8, 1144, 1145 |
| Bsa29I | ATCGAT | 1 cut(s) | 2546 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1343 |
| BsaJI | CCNNGG | 4 cut(s) | 38, 154, 1143, 2571 |
| BsaWI | WCCGGW | 1 cut(s) | 1012 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2228, 2258 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 750, 1067, 1118, 2013 |
| Bse1I | ACTGG | 7 cut(s) | 574, 1000, 1129, 1747, 1919, 2150, 2362 |
| Bse21I | CCTNAGG | 2 cut(s) | 1506, 2468 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1343 |
| BseAI | TCCGGA | 1 cut(s) | 1012 |
| BseBI | CCWGG | 3 cut(s) | 507, 580, 882 |
| BseCI | ATCGAT | 1 cut(s) | 2546 |
| BseDI | CCNNGG | 4 cut(s) | 38, 154, 1143, 2571 |
| BseJI | GATNNNNATC | 1 cut(s) | 1343 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 750, 1067, 1118, 2013 |
| BseMII | CTCAG | 7 cut(s) | 548, 1317, 1409, 1497, 1637, 2280, 2459 |
| BseNI | ACTGG | 7 cut(s) | 574, 1000, 1129, 1747, 1919, 2150, 2362 |
| BseRI | GAGGAG | 2 cut(s) | 1463, 2249 |
| BseXI | GCAGC | 7 cut(s) | 667, 670, 1243, 1561, 1818, 2342, 2546 |
| BseYI | CCCAGC | 1 cut(s) | 521 |
| BsgI | GTGCAG | 2 cut(s) | 1838, 2076 |
| BshFI | GGCC | 4 cut(s) | 429, 909, 1690, 2466 |
| BshNI | GGYRCC | 1 cut(s) | 644 |
| BshVI | ATCGAT | 1 cut(s) | 2546 |
| BsiHKAI | GWGCWC | 1 cut(s) | 87 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1143 |
| BsiSI | CCGG | 3 cut(s) | 7, 1013, 1144 |
| BslFI | GGGAC | 2 cut(s) | 467, 2210 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 750, 1067, 1118, 2013 |
| BsmAI | GTCTC | 3 cut(s) | 273, 674, 1272 |
| BsmBI | CGTCTC | 1 cut(s) | 674 |
| BsmFI | GGGAC | 2 cut(s) | 467, 2210 |
| BsmI | GAATGC | 4 cut(s) | 1609, 1715, 2170, 2644 |
| BsnI | GGCC | 4 cut(s) | 429, 909, 1690, 2466 |
| BsoBI | CYCGRG | 1 cut(s) | 1143 |
| Bsp119I | TTCGAA | 1 cut(s) | 1167 |
| Bsp1286I | GDGCHC | 2 cut(s) | 87, 1383 |
| Bsp13I | TCCGGA | 1 cut(s) | 1012 |
| Bsp143I | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| Bsp19I | CCATGG | 2 cut(s) | 38, 2571 |
| BspACI | CCGC | 5 cut(s) | 117, 395, 472, 475, 2057 |
| BspANI | GGCC | 4 cut(s) | 429, 909, 1690, 2466 |
| BspCNI | CTCAG | 7 cut(s) | 547, 1318, 1408, 1498, 1636, 2279, 2460 |
| BspDI | ATCGAT | 1 cut(s) | 2546 |
| BspEI | TCCGGA | 1 cut(s) | 1012 |
| BspLI | GGNNCC | 4 cut(s) | 646, 879, 1380, 1629 |
| BspPI | GGATC | 4 cut(s) | 1103, 1148, 1910, 2408 |
| BspQI | GCTCTTC | 1 cut(s) | 2070 |
| BspT104I | TTCGAA | 1 cut(s) | 1167 |
| BspT107I | GGYRCC | 1 cut(s) | 644 |
| BsrI | ACTGG | 7 cut(s) | 574, 1000, 1129, 1747, 1919, 2150, 2362 |
| BssECI | CCNNGG | 4 cut(s) | 38, 154, 1143, 2571 |
| BssMI | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| BssT1I | CCWWGG | 2 cut(s) | 38, 2571 |
| Bst2UI | CCWGG | 3 cut(s) | 507, 580, 882 |
| Bst4CI | ACNGT | 8 cut(s) | 155, 264, 641, 1466, 1521, 2097, 2201, 2296 |
| Bst6I | CTCTTC | 6 cut(s) | 47, 276, 1551, 1670, 2070, 2499 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1481, 1574, 2429 |
| BstBI | TTCGAA | 1 cut(s) | 1167 |
| BstC8I | GCNNGC | 3 cut(s) | 470, 1178, 1688 |
| BstDEI | CTNAG | 9 cut(s) | 369, 534, 1326, 1395, 1506, 1623, 2266, 2334, 2468 |
| BstDSI | CCRYGG | 3 cut(s) | 38, 154, 2571 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 748 |
| BstH2I | RGCGCY | 1 cut(s) | 1183 |
| BstHHI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| BstKTI | GATC | 6 cut(s) | 6, 589, 1111, 1156, 1918, 2416 |
| BstMAI | GTCTC | 3 cut(s) | 273, 674, 1272 |
| BstMBI | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 113, 190, 527, 1481, 1574, 2336, 2429 |
| BstNI | CCWGG | 3 cut(s) | 507, 580, 882 |
| BstNSI | RCATGY | 1 cut(s) | 1052 |
| BstSCI | CCNGG | 6 cut(s) | 6, 505, 578, 880, 1142, 1143 |
| BstSFI | CTRYAG | 2 cut(s) | 147, 775 |
| BstV1I | GCAGC | 7 cut(s) | 667, 670, 1243, 1561, 1818, 2342, 2546 |
| BstV2I | GAAGAC | 3 cut(s) | 1710, 1719, 2513 |
| Bsu15I | ATCGAT | 1 cut(s) | 2546 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1506, 2468 |
| BsuI | GTATCC | 2 cut(s) | 402, 2253 |
| BsuRI | GGCC | 4 cut(s) | 429, 909, 1690, 2466 |
| BsuTUI | ATCGAT | 1 cut(s) | 2546 |
| BtgI | CCRYGG | 3 cut(s) | 38, 154, 2571 |
| BtsI | GCAGTG | 2 cut(s) | 1575, 2088 |
| BtsIMutI | CAGTG | 8 cut(s) | 637, 1072, 1209, 1403, 1575, 2088, 2143, 2261 |
| Cac8I | GCNNGC | 3 cut(s) | 470, 1178, 1688 |
| CaiI | CAGNNNCTG | 2 cut(s) | 569, 1574 |
| CfoI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| Cfr13I | GGNCC | 2 cut(s) | 514, 1627 |
| Cfr9I | CCCGGG | 1 cut(s) | 1143 |
| ClaI | ATCGAT | 1 cut(s) | 2546 |
| Csp6I | GTAC | 4 cut(s) | 953, 1815, 2270, 2359 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 285, 320, 714, 749, 2032, 2067 |
| CviQI | GTAC | 4 cut(s) | 953, 1815, 2270, 2359 |
| DdeI | CTNAG | 9 cut(s) | 369, 534, 1326, 1395, 1506, 1623, 2266, 2334, 2468 |
| DpnI | GATC | 6 cut(s) | 5, 588, 1110, 1155, 1917, 2415 |
| DpnII | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| EaeI | YGGCCR | 1 cut(s) | 1688 |
| Eam1104I | CTCTTC | 6 cut(s) | 47, 276, 1551, 1670, 2070, 2499 |
| EarI | CTCTTC | 6 cut(s) | 47, 276, 1551, 1670, 2070, 2499 |
| EciI | GGCGGA | 2 cut(s) | 384, 490 |
| Ecl136II | GAGCTC | 1 cut(s) | 85 |
| Eco130I | CCWWGG | 2 cut(s) | 38, 2571 |
| Eco24I | GRGCYC | 2 cut(s) | 87, 1383 |
| Eco32I | GATATC | 1 cut(s) | 2138 |
| Eco47I | GGWCC | 2 cut(s) | 514, 1627 |
| Eco53kI | GAGCTC | 1 cut(s) | 85 |
| Eco57I | CTGAAG | 4 cut(s) | 113, 893, 1311, 1968 |
| Eco81I | CCTNAGG | 2 cut(s) | 1506, 2468 |
| Eco88I | CYCGRG | 1 cut(s) | 1143 |
| EcoICRI | GAGCTC | 1 cut(s) | 85 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 748 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1627 |
| EcoRII | CCWGG | 3 cut(s) | 505, 578, 880 |
| EcoRV | GATATC | 1 cut(s) | 2138 |
| EcoT14I | CCWWGG | 2 cut(s) | 38, 2571 |
| EcoT22I | ATGCAT | 2 cut(s) | 73, 1609 |
| EcoT38I | GRGCYC | 2 cut(s) | 87, 1383 |
| ErhI | CCWWGG | 2 cut(s) | 38, 2571 |
| Esp3I | CGTCTC | 1 cut(s) | 674 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 362, 394 |
| FaqI | GGGAC | 2 cut(s) | 467, 2210 |
| FauNDI | CATATG | 1 cut(s) | 247 |
| FblI | GTMKAC | 1 cut(s) | 1619 |
| Fnu4HI | GCNGC | 9 cut(s) | 117, 473, 656, 659, 1232, 1575, 1832, 2331, 2535 |
| FriOI | GRGCYC | 2 cut(s) | 87, 1383 |
| Fsp4HI | GCNGC | 9 cut(s) | 117, 473, 656, 659, 1232, 1575, 1832, 2331, 2535 |
| FspBI | CTAG | 4 cut(s) | 425, 1359, 1563, 2654 |
| GlaI | GCGC | 3 cut(s) | 26, 1149, 1181 |
| GluI | GCNGC | 9 cut(s) | 117, 473, 656, 659, 1232, 1575, 1832, 2331, 2535 |
| GsaI | CCCAGC | 1 cut(s) | 525 |
| HaeII | RGCGCY | 1 cut(s) | 1183 |
| HaeIII | GGCC | 4 cut(s) | 429, 909, 1690, 2466 |
| HapII | CCGG | 3 cut(s) | 7, 1013, 1144 |
| HhaI | GCGC | 3 cut(s) | 27, 1150, 1182 |
| Hin6I | GCGC | 3 cut(s) | 25, 1148, 1180 |
| HinP1I | GCGC | 3 cut(s) | 25, 1148, 1180 |
| HindIII | AAGCTT | 4 cut(s) | 125, 182, 1100, 1964 |
| HinfI | GANTC | 6 cut(s) | 241, 842, 1077, 1616, 1864, 2309 |
| HpaII | CCGG | 3 cut(s) | 7, 1013, 1144 |
| HphI | GGTGA | 5 cut(s) | 221, 1001, 1297, 1454, 1817 |
| Hpy166II | GTNNAC | 5 cut(s) | 19, 841, 1620, 1955, 2651 |
| Hpy188I | TCNGA | 5 cut(s) | 402, 565, 829, 2187, 2326 |
| Hpy8I | GTNNAC | 5 cut(s) | 19, 841, 1620, 1955, 2651 |
| HpyAV | CCTTC | 9 cut(s) | 51, 855, 868, 938, 1300, 1943, 2006, 2378, 2503 |
| HpyCH4III | ACNGT | 8 cut(s) | 155, 264, 641, 1466, 1521, 2097, 2201, 2296 |
| HpyCH4IV | ACGT | 3 cut(s) | 866, 1264, 2518 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 113, 190, 527, 1481, 1574, 2336, 2429 |
| HpyF3I | CTNAG | 9 cut(s) | 369, 534, 1326, 1395, 1506, 1623, 2266, 2334, 2468 |
| HpySE526I | ACGT | 3 cut(s) | 866, 1264, 2518 |
| HspAI | GCGC | 3 cut(s) | 25, 1148, 1180 |
| Kpn2I | TCCGGA | 1 cut(s) | 1012 |
| Kzo9I | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| LguI | GCTCTTC | 1 cut(s) | 2070 |
| LmnI | GCTCC | 3 cut(s) | 812, 1378, 2236 |
| Lsp1109I | GCAGC | 7 cut(s) | 667, 670, 1243, 1561, 1818, 2342, 2546 |
| LweI | GCATC | 8 cut(s) | 346, 1018, 1042, 1084, 1248, 1861, 2050, 2317 |
| MaeI | CTAG | 4 cut(s) | 425, 1359, 1563, 2654 |
| MaeII | ACGT | 3 cut(s) | 866, 1264, 2518 |
| MaeIII | GTNAC | 5 cut(s) | 406, 867, 1285, 1364, 1460 |
| MalI | GATC | 6 cut(s) | 5, 588, 1110, 1155, 1917, 2415 |
| MboI | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| MfeI | CAATTG | 2 cut(s) | 796, 2283 |
| MhlI | GDGCHC | 2 cut(s) | 87, 1383 |
| MlsI | TGGCCA | 1 cut(s) | 1690 |
| MluCI | AATT | 8 cut(s) | 796, 831, 1115, 1247, 1583, 1791, 2223, 2283 |
| MluNI | TGGCCA | 1 cut(s) | 1690 |
| MlyI | GAGTC | 3 cut(s) | 836, 1625, 2318 |
| MmeI | TCCRAC | 3 cut(s) | 1503, 1881, 2441 |
| Mox20I | TGGCCA | 1 cut(s) | 1690 |
| Mph1103I | ATGCAT | 2 cut(s) | 73, 1609 |
| MroI | TCCGGA | 1 cut(s) | 1012 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2027 |
| MscI | TGGCCA | 1 cut(s) | 1690 |
| MseI | TTAA | 5 cut(s) | 1332, 1610, 1710, 2226, 2671 |
| MslI | CAYNNNNRTG | 6 cut(s) | 413, 1014, 1038, 1442, 1781, 2529 |
| Msp20I | TGGCCA | 1 cut(s) | 1690 |
| MspI | CCGG | 3 cut(s) | 7, 1013, 1144 |
| MspR9I | CCNGG | 6 cut(s) | 8, 507, 580, 882, 1144, 1145 |
| MunI | CAATTG | 2 cut(s) | 796, 2283 |
| Mva1269I | GAATGC | 4 cut(s) | 1609, 1715, 2170, 2644 |
| MvaI | CCWGG | 3 cut(s) | 507, 580, 882 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 113, 190, 527, 1481, 1574, 2336, 2429 |
| NciI | CCSGG | 3 cut(s) | 8, 1144, 1145 |
| NcoI | CCATGG | 2 cut(s) | 38, 2571 |
| NdeI | CATATG | 1 cut(s) | 247 |
| NdeII | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| NlaIV | GGNNCC | 4 cut(s) | 646, 879, 1380, 1629 |
| NmuCI | GTSAC | 3 cut(s) | 406, 1285, 1460 |
| NsiI | ATGCAT | 2 cut(s) | 73, 1609 |
| NspI | RCATGY | 1 cut(s) | 1052 |
| NspV | TTCGAA | 1 cut(s) | 1167 |
| OliI | CACNNNNGTG | 1 cut(s) | 413 |
| PciSI | GCTCTTC | 1 cut(s) | 2070 |
| PctI | GAATGC | 4 cut(s) | 1609, 1715, 2170, 2644 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2027 |
| PfeI | GAWTC | 3 cut(s) | 241, 1077, 1864 |
| PflFI | GACNNNGTC | 1 cut(s) | 2296 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1118 |
| PkrI | GCNGC | 9 cut(s) | 118, 474, 657, 660, 1233, 1576, 1833, 2332, 2536 |
| PleI | GAGTC | 3 cut(s) | 836, 1624, 2317 |
| PpsI | GAGTC | 3 cut(s) | 836, 1624, 2317 |
| PpuMI | RGGWCCY | 1 cut(s) | 1627 |
| PshBI | ATTAAT | 1 cut(s) | 2226 |
| PsiI | TTATAA | 3 cut(s) | 759, 1269, 2409 |
| Psp124BI | GAGCTC | 1 cut(s) | 87 |
| Psp5II | RGGWCCY | 1 cut(s) | 1627 |
| Psp6I | CCWGG | 3 cut(s) | 505, 578, 880 |
| PspFI | CCCAGC | 1 cut(s) | 521 |
| PspGI | CCWGG | 3 cut(s) | 505, 578, 880 |
| PspN4I | GGNNCC | 4 cut(s) | 646, 879, 1380, 1629 |
| PspPI | GGNCC | 2 cut(s) | 514, 1627 |
| PspPPI | RGGWCCY | 1 cut(s) | 1627 |
| PstNI | CAGNNNCTG | 2 cut(s) | 569, 1574 |
| PsyI | GACNNNGTC | 1 cut(s) | 2296 |
| RsaI | GTAC | 4 cut(s) | 954, 1816, 2271, 2360 |
| RsaNI | GTAC | 4 cut(s) | 953, 1815, 2270, 2359 |
| RseI | CAYNNNNRTG | 6 cut(s) | 413, 1014, 1038, 1442, 1781, 2529 |
| SacI | GAGCTC | 1 cut(s) | 87 |
| SapI | GCTCTTC | 1 cut(s) | 2070 |
| SaqAI | TTAA | 5 cut(s) | 1332, 1610, 1710, 2226, 2671 |
| SatI | GCNGC | 9 cut(s) | 117, 473, 656, 659, 1232, 1575, 1832, 2331, 2535 |
| Sau3AI | GATC | 6 cut(s) | 3, 586, 1108, 1153, 1915, 2413 |
| Sau96I | GGNCC | 2 cut(s) | 514, 1627 |
| ScaI | AGTACT | 1 cut(s) | 2271 |
| SchI | GAGTC | 3 cut(s) | 836, 1625, 2318 |
| ScrFI | CCNGG | 6 cut(s) | 8, 507, 580, 882, 1144, 1145 |
| SduI | GDGCHC | 2 cut(s) | 87, 1383 |
| SfaNI | GCATC | 8 cut(s) | 346, 1018, 1042, 1084, 1248, 1861, 2050, 2317 |
| SfcI | CTRYAG | 2 cut(s) | 147, 775 |
| SfuI | TTCGAA | 1 cut(s) | 1167 |
| SinI | GGWCC | 2 cut(s) | 514, 1627 |
| SmaI | CCCGGG | 1 cut(s) | 1145 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 413, 1014, 1038, 1442, 1781, 2529 |
| SmlI | CTYRAG | 2 cut(s) | 1190, 1298 |
| SmoI | CTYRAG | 2 cut(s) | 1190, 1298 |
| Sse9I | AATT | 8 cut(s) | 796, 831, 1115, 1247, 1583, 1791, 2223, 2283 |
| SsiI | CCGC | 5 cut(s) | 117, 395, 472, 475, 2057 |
| SspMI | CTAG | 4 cut(s) | 425, 1359, 1563, 2654 |
| SstI | GAGCTC | 1 cut(s) | 87 |
| StyD4I | CCNGG | 6 cut(s) | 6, 505, 578, 880, 1142, 1143 |
| StyI | CCWWGG | 2 cut(s) | 38, 2571 |
| TaaI | ACNGT | 8 cut(s) | 155, 264, 641, 1466, 1521, 2097, 2201, 2296 |
| TaiI | ACGT | 3 cut(s) | 869, 1267, 2521 |
| TaqI | TCGA | 4 cut(s) | 692, 1167, 2140, 2546 |
| TasI | AATT | 8 cut(s) | 796, 831, 1115, 1247, 1583, 1791, 2223, 2283 |
| TatI | WGTACW | 1 cut(s) | 2269 |
| TauI | GCSGC | 2 cut(s) | 119, 475 |
| TfiI | GAWTC | 3 cut(s) | 241, 1077, 1864 |
| Tru1I | TTAA | 5 cut(s) | 1332, 1610, 1710, 2226, 2671 |
| Tru9I | TTAA | 5 cut(s) | 1332, 1610, 1710, 2226, 2671 |
| TscAI | CASTG | 8 cut(s) | 644, 1072, 1216, 1403, 1575, 2095, 2150, 2261 |
| TseFI | GTSAC | 3 cut(s) | 406, 1285, 1460 |
| TseI | GCWGC | 7 cut(s) | 655, 658, 1231, 1574, 1831, 2330, 2534 |
| Tsp45I | GTSAC | 3 cut(s) | 406, 1285, 1460 |
| TspGWI | ACGGA | 3 cut(s) | 1050, 1713, 2232 |
| TspMI | CCCGGG | 1 cut(s) | 1143 |
| TspRI | CASTG | 8 cut(s) | 644, 1072, 1216, 1403, 1575, 2095, 2150, 2261 |
| Tth111I | GACNNNGTC | 1 cut(s) | 2296 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1118 |
| VpaK11BI | GGWCC | 2 cut(s) | 514, 1627 |
| VspI | ATTAAT | 1 cut(s) | 2226 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 748 |
| XapI | RAATTY | 3 cut(s) | 1115, 1247, 1583 |
| XbaI | TCTAGA | 1 cut(s) | 1358 |
| XceI | RCATGY | 1 cut(s) | 1052 |
| XmaI | CCCGGG | 1 cut(s) | 1143 |
| XmiI | GTMKAC | 1 cut(s) | 1619 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2027 |
| XspI | CTAG | 4 cut(s) | 425, 1359, 1563, 2654 |
| ZrmI | AGTACT | 1 cut(s) | 2271 |
| Zsp2I | ATGCAT | 2 cut(s) | 73, 1609 |