Belongs to the class-I aminoacyl-tRNA synthetase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Transferase | PF02458 | 10 - 435 | 2e-74 | Transferase family |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 56, 797 |
| AatII | GACGTC | 1 cut(s) | 1310 |
| Acc36I | ACCTGC | 2 cut(s) | 236, 1189 |
| AccB1I | GGYRCC | 1 cut(s) | 1249 |
| AccI | GTMKAC | 2 cut(s) | 637, 901 |
| AccII | CGCG | 1 cut(s) | 489 |
| AccIII | TCCGGA | 1 cut(s) | 844 |
| AciI | CCGC | 3 cut(s) | 365, 692, 820 |
| AclWI | GGATC | 1 cut(s) | 611 |
| AcvI | CACGTG | 1 cut(s) | 884 |
| AcyI | GRCGYC | 1 cut(s) | 1307 |
| AdeI | CACNNNGTG | 1 cut(s) | 504 |
| AfaI | GTAC | 2 cut(s) | 1040, 1129 |
| AfeI | AGCGCT | 1 cut(s) | 683 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 193, 245, 409 |
| AflIII | ACRYGT | 1 cut(s) | 632 |
| AgeI | ACCGGT | 1 cut(s) | 136 |
| AgsI | TTSAA | 4 cut(s) | 168, 535, 557, 607 |
| AjnI | CCWGG | 1 cut(s) | 514 |
| AleI | CACNNNNGTG | 1 cut(s) | 586 |
| AluBI | AGCT | 2 cut(s) | 277, 947 |
| AluI | AGCT | 2 cut(s) | 277, 947 |
| AlwI | GGATC | 1 cut(s) | 611 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 950 |
| Ama87I | CYCGRG | 4 cut(s) | 338, 403, 612, 1298 |
| Aor13HI | TCCGGA | 1 cut(s) | 844 |
| Aor51HI | AGCGCT | 1 cut(s) | 683 |
| AoxI | GGCC | 7 cut(s) | 68, 118, 184, 263, 741, 1079, 1188 |
| ApeKI | GCWGC | 1 cut(s) | 947 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 80, 112 |
| AseI | ATTAAT | 1 cut(s) | 1353 |
| AsiGI | ACCGGT | 1 cut(s) | 136 |
| AspLEI | GCGC | 4 cut(s) | 489, 514, 684, 894 |
| AspS9I | GGNCC | 6 cut(s) | 185, 263, 523, 725, 741, 1178 |
| AsuC2I | CCSGG | 2 cut(s) | 404, 405 |
| AsuHPI | GGTGA | 3 cut(s) | 208, 460, 1018 |
| AvaI | CYCGRG | 4 cut(s) | 338, 403, 612, 1298 |
| AvaII | GGWCC | 3 cut(s) | 523, 725, 1178 |
| AxyI | CCTNAGG | 1 cut(s) | 122 |
| BanI | GGYRCC | 1 cut(s) | 1249 |
| BanII | GRGCYC | 1 cut(s) | 410 |
| BauI | CACGAG | 1 cut(s) | 78 |
| BbrPI | CACGTG | 1 cut(s) | 884 |
| BbsI | GAAGAC | 2 cut(s) | 899, 1310 |
| BbvI | GCAGC | 1 cut(s) | 934 |
| BccI | CCATC | 5 cut(s) | 407, 544, 580, 1071, 1174 |
| BceAI | ACGGC | 2 cut(s) | 940, 1175 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 916, 950 |
| BciT130I | CCWGG | 1 cut(s) | 516 |
| BclI | TGATCA | 1 cut(s) | 198 |
| BcnI | CCSGG | 2 cut(s) | 404, 405 |
| BfaI | CTAG | 1 cut(s) | 833 |
| BfoI | RGCGCY | 1 cut(s) | 685 |
| BfuAI | ACCTGC | 2 cut(s) | 236, 1189 |
| BglII | AGATCT | 2 cut(s) | 754, 1057 |
| BisI | GCNGC | 3 cut(s) | 693, 820, 948 |
| BlsI | GCNGC | 3 cut(s) | 694, 821, 949 |
| Bme1390I | CCNGG | 3 cut(s) | 404, 405, 516 |
| Bme18I | GGWCC | 3 cut(s) | 523, 725, 1178 |
| BmeT110I | CYCGRG | 4 cut(s) | 338, 403, 612, 1298 |
| BmgT120I | GGNCC | 6 cut(s) | 185, 263, 523, 725, 741, 1178 |
| BmiI | GGNNCC | 3 cut(s) | 187, 727, 1251 |
| BmrFI | CCNGG | 3 cut(s) | 404, 405, 516 |
| BmrI | ACTGGG | 1 cut(s) | 1107 |
| BmsI | GCATC | 5 cut(s) | 81, 343, 452, 499, 679 |
| BmuI | ACTGGG | 1 cut(s) | 1107 |
| BpiI | GAAGAC | 2 cut(s) | 899, 1310 |
| BpmI | CTGGAG | 1 cut(s) | 1019 |
| BpuMI | CCSGG | 2 cut(s) | 404, 405 |
| BsaAI | YACGTR | 1 cut(s) | 884 |
| BsaHI | GRCGYC | 1 cut(s) | 1307 |
| BsaJI | CCNNGG | 4 cut(s) | 403, 466, 1185, 1373 |
| BsaWI | WCCGGW | 2 cut(s) | 136, 844 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 288, 318 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 193, 245, 409 |
| Bse118I | RCCGGY | 1 cut(s) | 136 |
| Bse1I | ACTGG | 5 cut(s) | 584, 1002, 1102, 1135, 1253 |
| Bse21I | CCTNAGG | 1 cut(s) | 122 |
| Bse3DI | GCAATG | 3 cut(s) | 482, 934, 1134 |
| BseAI | TCCGGA | 1 cut(s) | 844 |
| BseBI | CCWGG | 1 cut(s) | 516 |
| BseDI | CCNNGG | 4 cut(s) | 403, 466, 1185, 1373 |
| BseGI | GGATG | 3 cut(s) | 418, 1082, 1371 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 193, 245, 409 |
| BseMI | GCAATG | 3 cut(s) | 482, 934, 1134 |
| BseMII | CTCAG | 2 cut(s) | 268, 687 |
| BseNI | ACTGG | 5 cut(s) | 584, 1002, 1102, 1135, 1253 |
| BseRI | GAGGAG | 1 cut(s) | 66 |
| BseXI | GCAGC | 1 cut(s) | 934 |
| BseYI | CCCAGC | 2 cut(s) | 42, 943 |
| Bsh1236I | CGCG | 1 cut(s) | 489 |
| BshFI | GGCC | 7 cut(s) | 70, 120, 186, 265, 743, 1081, 1190 |
| BshNI | GGYRCC | 1 cut(s) | 1249 |
| BshTI | ACCGGT | 1 cut(s) | 136 |
| BsiHKCI | CYCGRG | 4 cut(s) | 338, 403, 612, 1298 |
| BsiSI | CCGG | 4 cut(s) | 62, 137, 404, 845 |
| BslFI | GGGAC | 2 cut(s) | 386, 711 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 193, 245, 409 |
| BsmFI | GGGAC | 2 cut(s) | 386, 711 |
| BsmI | GAATGC | 3 cut(s) | 417, 613, 932 |
| BsnI | GGCC | 7 cut(s) | 70, 120, 186, 265, 743, 1081, 1190 |
| BsoBI | CYCGRG | 4 cut(s) | 338, 403, 612, 1298 |
| Bsp1286I | GDGCHC | 1 cut(s) | 410 |
| Bsp13I | TCCGGA | 1 cut(s) | 844 |
| Bsp143I | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| BspACI | CCGC | 3 cut(s) | 365, 692, 820 |
| BspANI | GGCC | 7 cut(s) | 70, 120, 186, 265, 743, 1081, 1190 |
| BspCNI | CTCAG | 2 cut(s) | 267, 686 |
| BspEI | TCCGGA | 1 cut(s) | 844 |
| BspFNI | CGCG | 1 cut(s) | 489 |
| BspLI | GGNNCC | 3 cut(s) | 187, 727, 1251 |
| BspMI | ACCTGC | 2 cut(s) | 236, 1189 |
| BspPI | GGATC | 1 cut(s) | 611 |
| BspT107I | GGYRCC | 1 cut(s) | 1249 |
| BsrDI | GCAATG | 3 cut(s) | 482, 934, 1134 |
| BsrFI | RCCGGY | 1 cut(s) | 136 |
| BsrI | ACTGG | 5 cut(s) | 584, 1002, 1102, 1135, 1253 |
| BssAI | RCCGGY | 1 cut(s) | 136 |
| BssECI | CCNNGG | 4 cut(s) | 403, 466, 1185, 1373 |
| BssMI | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| BssNAI | GTATAC | 1 cut(s) | 638 |
| BssNI | GRCGYC | 1 cut(s) | 1307 |
| BssSI | CACGAG | 1 cut(s) | 78 |
| BssT1I | CCWWGG | 1 cut(s) | 1185 |
| Bst1107I | GTATAC | 1 cut(s) | 638 |
| Bst2BI | CACGAG | 1 cut(s) | 78 |
| Bst2UI | CCWGG | 1 cut(s) | 516 |
| Bst4CI | ACNGT | 4 cut(s) | 541, 826, 899, 1377 |
| Bst6I | CTCTTC | 1 cut(s) | 1029 |
| BstACI | GRCGYC | 1 cut(s) | 1307 |
| BstBAI | YACGTR | 1 cut(s) | 884 |
| BstC8I | GCNNGC | 1 cut(s) | 1262 |
| BstDEI | CTNAG | 4 cut(s) | 19, 122, 254, 673 |
| BstDSI | CCRYGG | 1 cut(s) | 1373 |
| BstEII | GGTNACC | 1 cut(s) | 448 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 243 |
| BstF5I | GGATG | 3 cut(s) | 418, 1082, 1371 |
| BstFNI | CGCG | 1 cut(s) | 489 |
| BstH2I | RGCGCY | 1 cut(s) | 685 |
| BstHHI | GCGC | 4 cut(s) | 489, 514, 684, 894 |
| BstKTI | GATC | 5 cut(s) | 201, 619, 757, 1049, 1060 |
| BstMBI | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 414, 944 |
| BstNI | CCWGG | 1 cut(s) | 516 |
| BstNSI | RCATGY | 1 cut(s) | 636 |
| BstPI | GGTNACC | 1 cut(s) | 448 |
| BstSCI | CCNGG | 3 cut(s) | 402, 403, 514 |
| BstUI | CGCG | 1 cut(s) | 489 |
| BstV1I | GCAGC | 1 cut(s) | 934 |
| BstV2I | GAAGAC | 2 cut(s) | 899, 1310 |
| BstX2I | RGATCY | 3 cut(s) | 616, 754, 1057 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 588 |
| BstYI | RGATCY | 3 cut(s) | 616, 754, 1057 |
| BstZ17I | GTATAC | 1 cut(s) | 638 |
| Bsu36I | CCTNAGG | 1 cut(s) | 122 |
| BsuRI | GGCC | 7 cut(s) | 70, 120, 186, 265, 743, 1081, 1190 |
| BtgI | CCRYGG | 1 cut(s) | 1373 |
| BtsCI | GGATG | 3 cut(s) | 418, 1082, 1371 |
| BtsIMutI | CAGTG | 4 cut(s) | 591, 995, 1056, 1260 |
| BveI | ACCTGC | 2 cut(s) | 236, 1189 |
| Cac8I | GCNNGC | 1 cut(s) | 1262 |
| CaiI | CAGNNNCTG | 1 cut(s) | 950 |
| CfoI | GCGC | 4 cut(s) | 489, 514, 684, 894 |
| Cfr10I | RCCGGY | 1 cut(s) | 136 |
| Cfr13I | GGNCC | 6 cut(s) | 185, 263, 523, 725, 741, 1178 |
| Cfr9I | CCCGGG | 1 cut(s) | 403 |
| Csp6I | GTAC | 2 cut(s) | 1039, 1128 |
| CspAI | ACCGGT | 1 cut(s) | 136 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 865, 900 |
| CviAII | CATG | 4 cut(s) | 633, 745, 867, 1042 |
| CviQI | GTAC | 2 cut(s) | 1039, 1128 |
| DdeI | CTNAG | 4 cut(s) | 19, 122, 254, 673 |
| DpnI | GATC | 5 cut(s) | 200, 618, 756, 1048, 1059 |
| DpnII | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| DraIII | CACNNNGTG | 1 cut(s) | 504 |
| Eam1104I | CTCTTC | 1 cut(s) | 1029 |
| EarI | CTCTTC | 1 cut(s) | 1029 |
| Eco130I | CCWWGG | 1 cut(s) | 1185 |
| Eco147I | AGGCCT | 1 cut(s) | 120 |
| Eco24I | GRGCYC | 1 cut(s) | 410 |
| Eco47I | GGWCC | 3 cut(s) | 523, 725, 1178 |
| Eco47III | AGCGCT | 1 cut(s) | 683 |
| Eco72I | CACGTG | 1 cut(s) | 884 |
| Eco81I | CCTNAGG | 1 cut(s) | 122 |
| Eco88I | CYCGRG | 4 cut(s) | 338, 403, 612, 1298 |
| Eco91I | GGTNACC | 1 cut(s) | 448 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 243 |
| EcoO109I | RGGNCCY | 2 cut(s) | 185, 263 |
| EcoO65I | GGTNACC | 1 cut(s) | 448 |
| EcoRII | CCWGG | 1 cut(s) | 514 |
| EcoT14I | CCWWGG | 1 cut(s) | 1185 |
| EcoT22I | ATGCAT | 2 cut(s) | 872, 934 |
| EcoT38I | GRGCYC | 1 cut(s) | 410 |
| ErhI | CCWWGG | 1 cut(s) | 1185 |
| FaeI | CATG | 4 cut(s) | 636, 748, 870, 1045 |
| FaqI | GGGAC | 2 cut(s) | 386, 711 |
| FatI | CATG | 4 cut(s) | 632, 744, 866, 1041 |
| FbaI | TGATCA | 1 cut(s) | 198 |
| FblI | GTMKAC | 2 cut(s) | 637, 901 |
| Fnu4HI | GCNGC | 3 cut(s) | 693, 820, 948 |
| FokI | GGATG | 3 cut(s) | 425, 1089, 1378 |
| FriOI | GRGCYC | 1 cut(s) | 410 |
| Fsp4HI | GCNGC | 3 cut(s) | 693, 820, 948 |
| FspBI | CTAG | 1 cut(s) | 833 |
| GlaI | GCGC | 4 cut(s) | 488, 513, 683, 893 |
| GluI | GCNGC | 3 cut(s) | 693, 820, 948 |
| GsaI | CCCAGC | 2 cut(s) | 46, 947 |
| GsuI | CTGGAG | 1 cut(s) | 1019 |
| HaeII | RGCGCY | 1 cut(s) | 685 |
| HaeIII | GGCC | 7 cut(s) | 70, 120, 186, 265, 743, 1081, 1190 |
| HapII | CCGG | 4 cut(s) | 62, 137, 404, 845 |
| HhaI | GCGC | 4 cut(s) | 489, 514, 684, 894 |
| Hin1I | GRCGYC | 1 cut(s) | 1307 |
| Hin1II | CATG | 4 cut(s) | 636, 748, 870, 1045 |
| Hin6I | GCGC | 4 cut(s) | 487, 512, 682, 892 |
| HinP1I | GCGC | 4 cut(s) | 487, 512, 682, 892 |
| HincII | GTYRAC | 1 cut(s) | 1147 |
| HindII | GTYRAC | 1 cut(s) | 1147 |
| HindIII | AAGCTT | 1 cut(s) | 275 |
| HinfI | GANTC | 1 cut(s) | 1369 |
| HpaII | CCGG | 4 cut(s) | 62, 137, 404, 845 |
| HphI | GGTGA | 3 cut(s) | 208, 460, 1018 |
| Hpy166II | GTNNAC | 5 cut(s) | 493, 638, 902, 1147, 1195 |
| Hpy188I | TCNGA | 8 cut(s) | 100, 132, 257, 412, 676, 688, 1117, 1312 |
| Hpy188III | TCNNGA | 9 cut(s) | 80, 338, 532, 604, 644, 845, 957, 1176, 1300 |
| Hpy8I | GTNNAC | 5 cut(s) | 493, 638, 902, 1147, 1195 |
| Hpy99I | CGWCG | 1 cut(s) | 1312 |
| HpyAV | CCTTC | 4 cut(s) | 174, 254, 293, 1006 |
| HpyCH4III | ACNGT | 4 cut(s) | 541, 826, 899, 1377 |
| HpyCH4IV | ACGT | 5 cut(s) | 330, 883, 904, 1126, 1307 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 414, 944 |
| HpyF3I | CTNAG | 4 cut(s) | 19, 122, 254, 673 |
| HpySE526I | ACGT | 5 cut(s) | 330, 883, 904, 1126, 1307 |
| Hsp92I | GRCGYC | 1 cut(s) | 1307 |
| Hsp92II | CATG | 4 cut(s) | 636, 748, 870, 1045 |
| HspAI | GCGC | 4 cut(s) | 487, 512, 682, 892 |
| Kpn2I | TCCGGA | 1 cut(s) | 844 |
| Ksp22I | TGATCA | 1 cut(s) | 198 |
| Kzo9I | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| LmnI | GCTCC | 1 cut(s) | 592 |
| Lsp1109I | GCAGC | 1 cut(s) | 934 |
| LweI | GCATC | 5 cut(s) | 81, 343, 452, 499, 679 |
| MaeI | CTAG | 1 cut(s) | 833 |
| MaeII | ACGT | 5 cut(s) | 330, 883, 904, 1126, 1307 |
| MaeIII | GTNAC | 3 cut(s) | 331, 448, 917 |
| MalI | GATC | 5 cut(s) | 200, 618, 756, 1048, 1059 |
| MboI | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| MboII | GAAGA | 5 cut(s) | 749, 760, 899, 1046, 1315 |
| MfeI | CAATTG | 2 cut(s) | 775, 968 |
| MflI | RGATCY | 3 cut(s) | 616, 754, 1057 |
| MhlI | GDGCHC | 1 cut(s) | 410 |
| MluCI | AATT | 5 cut(s) | 345, 564, 627, 775, 968 |
| MmeI | TCCRAC | 1 cut(s) | 1290 |
| Mph1103I | ATGCAT | 2 cut(s) | 872, 934 |
| MroI | TCCGGA | 1 cut(s) | 844 |
| MseI | TTAA | 3 cut(s) | 33, 840, 1353 |
| MslI | CAYNNNNRTG | 1 cut(s) | 586 |
| MspA1I | CMGCKG | 1 cut(s) | 947 |
| MspI | CCGG | 4 cut(s) | 62, 137, 404, 845 |
| MspR9I | CCNGG | 3 cut(s) | 404, 405, 516 |
| MunI | CAATTG | 2 cut(s) | 775, 968 |
| Mva1269I | GAATGC | 3 cut(s) | 417, 613, 932 |
| MvaI | CCWGG | 1 cut(s) | 516 |
| MvnI | CGCG | 1 cut(s) | 489 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 414, 944 |
| NciI | CCSGG | 2 cut(s) | 404, 405 |
| NdeII | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| NlaIII | CATG | 4 cut(s) | 636, 748, 870, 1045 |
| NlaIV | GGNNCC | 3 cut(s) | 187, 727, 1251 |
| NmeAIII | GCCGAG | 1 cut(s) | 491 |
| NmuCI | GTSAC | 1 cut(s) | 448 |
| NsiI | ATGCAT | 2 cut(s) | 872, 934 |
| NspI | RCATGY | 1 cut(s) | 636 |
| OliI | CACNNNNGTG | 1 cut(s) | 586 |
| PaeR7I | CTCGAG | 2 cut(s) | 338, 1298 |
| PceI | AGGCCT | 1 cut(s) | 120 |
| PciI | ACATGT | 1 cut(s) | 632 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 336 |
| PctI | GAATGC | 3 cut(s) | 417, 613, 932 |
| PfeI | GAWTC | 1 cut(s) | 1369 |
| PinAI | ACCGGT | 1 cut(s) | 136 |
| PkrI | GCNGC | 3 cut(s) | 694, 821, 949 |
| PmaCI | CACGTG | 1 cut(s) | 884 |
| PmlI | CACGTG | 1 cut(s) | 884 |
| Ppu21I | YACGTR | 1 cut(s) | 884 |
| PscI | ACATGT | 1 cut(s) | 632 |
| PshBI | ATTAAT | 1 cut(s) | 1353 |
| PsiI | TTATAA | 2 cut(s) | 56, 797 |
| Psp6I | CCWGG | 1 cut(s) | 514 |
| PspCI | CACGTG | 1 cut(s) | 884 |
| PspEI | GGTNACC | 1 cut(s) | 448 |
| PspFI | CCCAGC | 2 cut(s) | 42, 943 |
| PspGI | CCWGG | 1 cut(s) | 514 |
| PspN4I | GGNNCC | 3 cut(s) | 187, 727, 1251 |
| PspPI | GGNCC | 6 cut(s) | 185, 263, 523, 725, 741, 1178 |
| PstNI | CAGNNNCTG | 1 cut(s) | 950 |
| PsuI | RGATCY | 3 cut(s) | 616, 754, 1057 |
| PvuII | CAGCTG | 1 cut(s) | 947 |
| RsaI | GTAC | 2 cut(s) | 1040, 1129 |
| RsaNI | GTAC | 2 cut(s) | 1039, 1128 |
| RseI | CAYNNNNRTG | 1 cut(s) | 586 |
| SaqAI | TTAA | 3 cut(s) | 33, 840, 1353 |
| SatI | GCNGC | 3 cut(s) | 693, 820, 948 |
| Sau3AI | GATC | 5 cut(s) | 198, 616, 754, 1046, 1057 |
| Sau96I | GGNCC | 6 cut(s) | 185, 263, 523, 725, 741, 1178 |
| ScrFI | CCNGG | 3 cut(s) | 404, 405, 516 |
| SduI | GDGCHC | 1 cut(s) | 410 |
| SfaNI | GCATC | 5 cut(s) | 81, 343, 452, 499, 679 |
| Sfr274I | CTCGAG | 2 cut(s) | 338, 1298 |
| SinI | GGWCC | 3 cut(s) | 523, 725, 1178 |
| SlaI | CTCGAG | 2 cut(s) | 338, 1298 |
| SmaI | CCCGGG | 1 cut(s) | 405 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 586 |
| SmlI | CTYRAG | 2 cut(s) | 338, 1298 |
| SmoI | CTYRAG | 2 cut(s) | 338, 1298 |
| Sse9I | AATT | 5 cut(s) | 345, 564, 627, 775, 968 |
| SseBI | AGGCCT | 1 cut(s) | 120 |
| SsiI | CCGC | 3 cut(s) | 365, 692, 820 |
| SspI | AATATT | 1 cut(s) | 1351 |
| SspMI | CTAG | 1 cut(s) | 833 |
| StuI | AGGCCT | 1 cut(s) | 120 |
| StyD4I | CCNGG | 3 cut(s) | 402, 403, 514 |
| StyI | CCWWGG | 1 cut(s) | 1185 |
| TaaI | ACNGT | 4 cut(s) | 541, 826, 899, 1377 |
| TaiI | ACGT | 5 cut(s) | 333, 886, 907, 1129, 1310 |
| TaqI | TCGA | 5 cut(s) | 37, 173, 339, 789, 1299 |
| TasI | AATT | 5 cut(s) | 345, 564, 627, 775, 968 |
| TatI | WGTACW | 1 cut(s) | 1038 |
| TauI | GCSGC | 2 cut(s) | 695, 822 |
| TfiI | GAWTC | 1 cut(s) | 1369 |
| Tru1I | TTAA | 3 cut(s) | 33, 840, 1353 |
| Tru9I | TTAA | 3 cut(s) | 33, 840, 1353 |
| TscAI | CASTG | 4 cut(s) | 591, 1002, 1056, 1260 |
| TseFI | GTSAC | 1 cut(s) | 448 |
| TseI | GCWGC | 1 cut(s) | 947 |
| Tsp45I | GTSAC | 1 cut(s) | 448 |
| TspDTI | ATGAA | 9 cut(s) | 463, 681, 761, 855, 1025, 1038, 1043, 1047, 1096 |
| TspGWI | ACGGA | 1 cut(s) | 309 |
| TspMI | CCCGGG | 1 cut(s) | 403 |
| TspRI | CASTG | 4 cut(s) | 591, 1002, 1056, 1260 |
| VpaK11BI | GGWCC | 3 cut(s) | 523, 725, 1178 |
| VspI | ATTAAT | 1 cut(s) | 1353 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 243 |
| XceI | RCATGY | 1 cut(s) | 636 |
| XhoI | CTCGAG | 2 cut(s) | 338, 1298 |
| XmaI | CCCGGG | 1 cut(s) | 403 |
| XmiI | GTMKAC | 2 cut(s) | 637, 901 |
| XspI | CTAG | 1 cut(s) | 833 |
| ZraI | GACGTC | 1 cut(s) | 1308 |
| Zsp2I | ATGCAT | 2 cut(s) | 872, 934 |