Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000332

Physical Location & Seq

Genomic Coordinates Forward (+)

31767442 .. 31775270

Gene Structure 7829 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1256800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3225 bp

ATGTCTTCCCCAACTGCAGAGGCAGATACAATCAAGAAAATGAAGAAGGAAGCAAAGGCAAAAGAGAAAGAATTGAAAAAGCAAAAAGCTTTGCAGAAAGCTATCAAGCTGCAGGAGCAACAAGCATGTGCTTCTACTTTGAAGACAATTAAAACGAAGAATGCAAGGCTTTTGGATGATGAAGATGACAATGCAAGGGACCCGGAGACTCCATTCGGCCAGAAGAAAAGGATGGCCAGTCAAATGGCCAAAATGTACCATCCGCGTGCTGTGGAGAAGTCGTGGTATGAGTGGTGGGAGAAAATGGGGTTCTTCTCGGCAGATGCTAACAGCTCTAAACCTCCATTTGTGATTGTTTTACCGCCGCCCAATGTGACCGGCACCCTTCACATAGGCCATGCTCTTACTGCTGCTATTGAGGACACTATCATTCGTTGGCGGAGAATGTCCGGCTACAATGCCATGTGGGTGCCTGGAATGGATCATGCCGGGATAGCAACACAGGTGGTTGTTGAAAAGAAGCTTATGAGTGAAAAAAATTTAACCAGACATGATATTGGTCGTGAGCAATTTGTGGCTAAGGTTTGGGACTGGAAGAAAAAGCATGGGAGCACCATTTTACAGCAGCTCCGTCGCTTGGGTGCTTCTTTGGATTGGTCCCGTGGGTGCTTCACGATGGATGAGAAAAGGTCAAATGCCGTGACAGAGGCTTTTGTAAGGCTGCATAAGCAGGGGCTTCTCTACAGGGCTAATCGCATAGTGAATTGGGATTGTGTGTTGCGTACAGCTATATCTGACATTGAGGTGGATTACATCGATATAGAAGAAAGGACATTACTGAGGGTTCCTGGATACGATAGTCCTGTTGAATTTGGTGTGTTAACTTCATTTGCTTACCCTTTGGAGGAAGGTTTAGGTGAGATTGTGGTGGCCACCACTAGAGTGGAAACTATGCTCGGTGATACTGCCATTGCTGTACATCCTAATGATAAGAGGTATGAACATCTTCATGGAAAACATGCCATCCATCCTTTCAATGGACGAAGGATTTGTATAGTCCTTGATGAAATTGTTGTTGATCCAGAGTTCGGGACTGGTGCTGTGAAGATTACCCCAGCCCATGATCCGAATGATTTTAATGCGGGAAAGCGCCATAACCTGGACTTTATTAACATCTTTACGGATGACGGAAAAATAAACGAGGAGGGTGGAGAATTTGCAGGAATGCCACGTTTTGAAGCCCGAGAAGCAGTGACAGAAGCACTAAAGAAGAAGGGTCTCTTTAAAGAAGCAAAGAACAACGAGATGCGACTTGGAATTTGCTCTAGAAGCCAAGATGTTGTGGAACCCATGATAAAGCCCCAATGGTATCTTAAATGCAGCGGTATGGGAAAGCAAGCCCTTGATGCTGTCGTAGATGATGAACACAGGAAGCTTGAGATCATCCCAAGAGAGTATACTGCTGAATGGAAGAGATGGCTTGAGAACATTCGTGATTGGTGCATCTCGAGGCAACTTTGGTGGGGTCACCGTATTCCTGCATGGTATGTTGTTTATGAAGGCGATAAGCAGGAAGATTTTGGATTTATTGATGACCGATGGGTAATTGCTAGAAATGAGGAAGAGGCTCAAGCAAAGGCTAGTGATGCATATCAGGGGAATTTTCAATTAATTCAGGATCCTGATGTCCTTGACACATGGTTTTCTTCTGGTTTGTTTCCATTGTCCGTGTTGGGCTGGCCAGATAACACAGAAGATCTGAAGGCTTTTTATCCGACTGCAGTTCTTGAAACTGGACATGATATTCTCTTTTTCTGGGTAGCTCGTATGGTAATGCTAGGAATGACATTGGGTGGCAACATACCTTTTACAAAGGTATATTTGCACCCAATGATTCGTGATGCCCATGGACGTAAAATGTCAAAATCCTTGGGGAATGTCATTGATCCACTTGATGTAATAAATGGTATTGATCTTGAAGGTCTACGGAAGAAGCTTTTAGAGGGAAATTTGGATGCCAAGGAAGTAGCCGTCTCAGAAGAAGGGCTAAAGAAAGACTTTCCTGCAGGAATTTGGGAATGTGGTGCAGATGCTCTCCGTTTTGCTCTCGTGTCTTATACTGCTCAGCATGATAAGATTAATTTGGACATCCAAAGGGTTGAGAGCTATAGTTGCTGGTGTAATAAATTGTGGAATGCAGTGCGATTTTCTCTGAGCATACTTGGGGATGACTATGTTCCACCCTCAAGTGTAAATCCGGATTTGCTTCCATTTAGTTGCCAGTGGATACTCTCGGTACTGAATACATCCATATCCAAAATTATTTCGTCACTGGAATCCTATGAGTTATCAGATGCGGCCACCGCAGTGCATGCTTGGTGGCAATACCAGTTGTGCGATGTTTTTCTTGAAACTATCAAGCCTTACTTTTTTGGTAACGATCCAAAGTTTGCATCTGAAAGGGGTTTCGCACGGGATACACTATGGTTATGTCTTGAACATGGGTTACGGTTGCTTCATCCTTTTATGCCATTTGTCACAGAAGAACTGTGGCAACGTCTTCCATCTTCCGGGGTTCACAAGAGGGCAATATCAATTATGATGTGCGAGTATCCATCCATTGTAGATTGCTGGACAAATGAAAGGGTGGAGTCTGAGATGAACCTTGTAGAGACTGTTGTGAAATCTCTCCGTTCACTTGCAAAAGAAAGTCGTGAAAGGCGACCAGCTCATGTAATGTGTCGAAACAATTTGGATTGGAATATAATCTGCAGCCATCGACTGGAGATTGAAGCTCTTGCCCATTTGTCTTCTTTGACGATACTCAGCGAGAATGACAGTTTTCCCACCAGCACCGTCTCCAGTGGATATGAGATGTGTCTTGTAAATGAATATCTGTCGGTTTTTCTGAGGGTTCCAAGAGTAAAGGCAGACCCTGAAAAGATCCGCGAGAAGATGGAAAAATTCATAAGGGAACGGGAAAAGCTGTATCTGATGATAAACTCTCCTGGTTACCAAGAAAAGACATCTGAAAAGATTCAAGAATCCAACGCTGACAAGCTAGAATGCTTGAAGCAGGAAATAAGTACTCTGGGAAGGTTATGGTGGATGAATTCTCCTGGTTACCGAAAAAAGGCGTCCGAGGAAGATCAAGCATGTAGTGCTGACAATCTAGAATTCTTAGAGAAGGAAATAATGGAAAGGCTGCGATGCTCCTTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004832 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006438 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

1075

Amino Acids

123.15

Weight (kDa)

6.01

Isoelectric Point (pI)

44.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	95 - 715	1.3e-143	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	121 - 184	5.8e-07	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	337 - 396	3e-06	Leucyl-tRNA synthetase, editing domain
Anticodon_1	PF08264	761 - 903	1.4e-29	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14610 AT1G27160
fragaria_vesca	FvH4_5g33250 FvH4_7g01700 FvH4_7g28940 FvH4_7g28940 FvH4_7g28940
malus_domestica	MD01G1196000.v1.1 MD03G1000400.v1.1 MD06G1107900.v1.1 MD07G1262800.v1.1 MD15G1255900.v1.1 MD15G1433500.v1.1 MD17G1256800.v1.1
prunus_persica	Prupe.1G576900_v2.0.a1 Prupe.1G576900_v2.0.a1 Prupe.2G289400_v2.0.a1 Prupe.2G289400_v2.0.a1
pyrus_communis	pycom01g20660 pycom03g00060 pycom07g24190 pycom15g38270 pycom17g25900
rosa_chinensis	RchiOBHm_Chr1g0376611 RchiOBHm_Chr2g0151751 RchiOBHm_Chr2g0151761 RchiOBHm_Chr5g0049821 RchiOBHm_Chr5g0066661 RchiOBHm_Chr7g0182401 RchiOBHm_Chr7g0218391 RchiOBHm_Chr7g0218401 RchiOBHm_Chr7g0218411 RchiOBHm_Chr7g0232241 RchiOBHm_Chr7g0232251 RchiOBHm_Chr7g0232281 RchiOBHm_Chr7g0232291 RchiOBHm_Chr7g0232301
rosa_laevigata	RLG00000001360 RLG00000001362 RLG00000001368 RLG00000005125 RLG00000005126 RLG00000026593
rosa_multiflora	Rmu_co8187388.1_g000001 Rmu_co8244885.1_g000001 Rmu_co8371739.1_g000001 Rmu_co8421977.1_g000001 Rmu_co8457289.1_g000001 Rmu_sc0000239.1_g000041 Rmu_sc0000927.1_g000001 Rmu_sc0000945.1_g000003 Rmu_sc0002042.1_g000017 Rmu_sc0004136.1_g000003 Rmu_sc0005294.1_g000036 Rmu_sc0017871.1_g000001 Rmu_sc0029500.1_g000001 Rmu_sc0037577.1_g000001 Rmu_sc0037577.1_g000002 Rmu_ssc0000034.1_g000001 Rmu_ssc0000115.1_g000001
rosa_roxburghii	Rroxscaffold_2G00112130 Rroxscaffold_3G00228490 Rroxscaffold_3G00228520 Rroxscaffold_3G00228530 Rroxscaffold_3G00228620 Rroxscaffold_3G00228650 Rroxscaffold_4G00281940
rosa_rugosa	Rorug01G0396800 Rorug01G0396800 Rorug06G0073800 Rorug06G0073900 Rorug06G0447200 Rorug06G0447200 Rorug06G0447300 Rorug06G0447400 Rorug07G0270800 Rorug07G0271800 Rorug07G0271900 Rorug07G0272000 Rorug07G0272200 Rorug07G0272300
rosa_samantha	Rh1AG413400 Rh1BG373100 Rh1CG021500 Rh1CG151000 Rh1CG387000 Rh1DG404000 Rh2BG502300 Rh2DG513300 Rh3AG126000 Rh4AG156700 Rh7AG050700 Rh7AG050800 Rh7AG425000 Rh7AG425500 Rh7AG426200 Rh7BG050400 Rh7BG399800 Rh7BG399900 Rh7BG400000 Rh7CG052000 Rh7CG445400 Rh7CG445600 Rh7CG445900 Rh7CG446000 Rh7DG417300
rosa_wichuraiana	Rw0G007060 Rw1G036250 Rw1G036280 Rw7G027040 Rw7G035320 Rw7G035350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	380, 469
AccB7I	CCANNNNNTGG	2 cut(s)	1159, 2782
AccI	GTMKAC	2 cut(s)	1457, 1984
AccII	CGCG	2 cut(s)	265, 2949
AccIII	TCCGGA	1 cut(s)	2257
AciI	CCGC	9 cut(s)	263, 362, 365, 439, 1142, 1383, 2357, 2364, 2947
AclWI	GGATC	8 cut(s)	489, 1073, 1118, 1673, 1686, 1940, 2435, 2938
AcoI	YGGCCR	6 cut(s)	217, 234, 246, 930, 1739, 2358
AcuI	CTGAAG	1 cut(s)	1781
AcyI	GRCGYC	1 cut(s)	3137
AfaI	GTAC	5 cut(s)	257, 784, 978, 2298, 3088
AfiI	CCNNNNNNNGG	8 cut(s)	637, 904, 1037, 1088, 1159, 2570, 2782, 3133
AjnI	CCWGG	5 cut(s)	472, 847, 1158, 3007, 3118
AleI	CACNNNNGTG	2 cut(s)	941, 2366
Alw21I	GWGCWC	1 cut(s)	614
Alw26I	GTCTC	5 cut(s)	200, 1283, 2038, 2666, 2863
AlwI	GGATC	8 cut(s)	489, 1073, 1118, 1673, 1686, 1940, 2435, 2938
AlwNI	CAGNNNCTG	1 cut(s)	2936
Ama87I	CYCGRG	2 cut(s)	1242, 1507
Aor13HI	TCCGGA	1 cut(s)	2257
AoxI	GGCC	7 cut(s)	217, 234, 246, 394, 930, 1739, 2358
ApeKI	GCWGC	7 cut(s)	109, 410, 625, 721, 1380, 2772, 3205
ArsI	GACNNNNNNTTYG	4 cut(s)	1032, 1064, 1302, 1334
AseI	ATTAAT	2 cut(s)	1670, 2139
Asp700I	GAANNNNTTC	2 cut(s)	1005, 3036
AspLEI	GCGC	1 cut(s)	1152
AspS9I	GGNCC	2 cut(s)	199, 657
AsuC2I	CCSGG	3 cut(s)	203, 490, 2572
AsuHPI	GGTGA	3 cut(s)	929, 971, 1520
AvaI	CYCGRG	2 cut(s)	1242, 1507
AvaII	GGWCC	2 cut(s)	199, 657
BaeI	ACNNNNGTAYC	2 cut(s)	844, 877
BalI	TGGCCA	4 cut(s)	236, 248, 932, 1741
BamHI	GGATCC	1 cut(s)	1678
BanI	GGYRCC	2 cut(s)	380, 469
BauI	CACGAG	1 cut(s)	2108
BbsI	GAAGAC	3 cut(s)	149, 2552, 2802
Bbv12I	GWGCWC	1 cut(s)	614
BbvI	GCAGC	7 cut(s)	96, 397, 637, 708, 1392, 2784, 3192
BceAI	ACGGC	2 cut(s)	683, 2015
BcgI	CGANNNNNNTGC	2 cut(s)	614, 648
BciT130I	CCWGG	5 cut(s)	474, 849, 1160, 3009, 3120
BciVI	GTATCC	4 cut(s)	845, 2280, 2470, 2622
BcnI	CCSGG	3 cut(s)	203, 490, 2572
BcoDI	GTCTC	5 cut(s)	200, 1283, 2038, 2666, 2863
BfaI	CTAG	7 cut(s)	939, 1326, 1611, 1641, 1838, 3062, 3173
BfmI	CTRYAG	7 cut(s)	15, 110, 742, 1779, 2064, 2167, 2770
BfoI	RGCGCY	1 cut(s)	1153
BfuI	GTATCC	4 cut(s)	845, 2280, 2470, 2622
BglII	AGATCT	1 cut(s)	1756
BisI	GCNGC	9 cut(s)	110, 365, 411, 626, 722, 1381, 2358, 2773, 3206
BlpI	GCTNAGC	1 cut(s)	2124
BlsI	GCNGC	9 cut(s)	111, 366, 412, 627, 723, 1382, 2359, 2774, 3207
BmcAI	AGTACT	1 cut(s)	3088
Bme1390I	CCNGG	8 cut(s)	203, 474, 490, 849, 1160, 2572, 3009, 3120
Bme18I	GGWCC	2 cut(s)	199, 657
BmeT110I	CYCGRG	2 cut(s)	1242, 1507
BmgT120I	GGNCC	2 cut(s)	199, 657
BmiI	GGNNCC	9 cut(s)	200, 201, 382, 471, 659, 846, 1347, 1680, 2916
BmrFI	CCNGG	8 cut(s)	203, 474, 490, 849, 1160, 2572, 3009, 3120
BpiI	GAAGAC	3 cut(s)	149, 2552, 2802
BpmI	CTGGAG	2 cut(s)	2804, 2845
Bpu10I	CCTNAGC	1 cut(s)	579
Bpu1102I	GCTNAGC	1 cut(s)	2124
BpuEI	CTTGAG	4 cut(s)	1457, 1502, 1614, 2230
BpuMI	CCSGG	3 cut(s)	203, 490, 2572
Bsa29I	ATCGAT	1 cut(s)	816
BsaBI	GATNNNNATC	1 cut(s)	1650
BsaHI	GRCGYC	1 cut(s)	3137
BsaI	GGTCTC	1 cut(s)	1283
BsaJI	CCNNGG	6 cut(s)	661, 1906, 1929, 2019, 2571, 3141
BsaWI	WCCGGW	1 cut(s)	2257
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 612, 642
Bsc4I	CCNNNNNNNGG	8 cut(s)	637, 904, 1037, 1088, 1159, 2570, 2782, 3133
Bse118I	RCCGGY	1 cut(s)	377
Bse1I	ACTGG	9 cut(s)	237, 596, 1099, 1798, 2281, 2337, 2389, 2787, 2862
Bse3DI	GCAATG	1 cut(s)	969
Bse8I	GATNNNNATC	1 cut(s)	1650
BseAI	TCCGGA	1 cut(s)	2257
BseBI	CCWGG	5 cut(s)	474, 849, 1160, 3009, 3120
BseCI	ATCGAT	1 cut(s)	816
BseDI	CCNNGG	6 cut(s)	661, 1906, 1929, 2019, 2571, 3141
BseJI	GATNNNNATC	1 cut(s)	1650
BseLI	CCNNNNNNNGG	8 cut(s)	637, 904, 1037, 1088, 1159, 2570, 2782, 3133
BseMI	GCAATG	1 cut(s)	969
BseMII	CTCAG	7 cut(s)	830, 2049, 2138, 2204, 2646, 2839, 2900
BseNI	ACTGG	9 cut(s)	237, 596, 1099, 1798, 2281, 2337, 2389, 2787, 2862
BseRI	GAGGAG	1 cut(s)	1217
BseXI	GCAGC	7 cut(s)	96, 397, 637, 708, 1392, 2784, 3192
BseYI	CCCAGC	1 cut(s)	1114
BsgI	GTGCAG	1 cut(s)	2106
Bsh1236I	CGCG	2 cut(s)	265, 2949
BshFI	GGCC	7 cut(s)	219, 236, 248, 396, 932, 1741, 2360
BshNI	GGYRCC	2 cut(s)	380, 469
BshVI	ATCGAT	1 cut(s)	816
BsiHKAI	GWGCWC	1 cut(s)	614
BsiHKCI	CYCGRG	2 cut(s)	1242, 1507
BsiSI	CCGG	6 cut(s)	203, 378, 450, 489, 2258, 2571
BslFI	GGGAC	4 cut(s)	212, 602, 643, 1105
BslI	CCNNNNNNNGG	8 cut(s)	637, 904, 1037, 1088, 1159, 2570, 2782, 3133
BsmAI	GTCTC	5 cut(s)	200, 1283, 2038, 2666, 2863
BsmBI	CGTCTC	2 cut(s)	2038, 2863
BsmFI	GGGAC	4 cut(s)	212, 602, 643, 1105
BsmI	GAATGC	4 cut(s)	166, 1230, 2200, 3071
BsnI	GGCC	7 cut(s)	219, 236, 248, 396, 932, 1741, 2360
Bso31I	GGTCTC	1 cut(s)	1283
BsoBI	CYCGRG	2 cut(s)	1242, 1507
Bsp1286I	GDGCHC	1 cut(s)	614
Bsp13I	TCCGGA	1 cut(s)	2257
Bsp1407I	TGTACA	1 cut(s)	976
Bsp1720I	GCTNAGC	1 cut(s)	2124
Bsp19I	CCATGG	1 cut(s)	1906
BspACI	CCGC	9 cut(s)	263, 362, 365, 439, 1142, 1383, 2357, 2364, 2947
BspANI	GGCC	7 cut(s)	219, 236, 248, 396, 932, 1741, 2360
BspCNI	CTCAG	7 cut(s)	831, 2048, 2137, 2205, 2647, 2838, 2901
BspDI	ATCGAT	1 cut(s)	816
BspEI	TCCGGA	1 cut(s)	2257
BspFNI	CGCG	2 cut(s)	265, 2949
BspLI	GGNNCC	9 cut(s)	200, 201, 382, 471, 659, 846, 1347, 1680, 2916
BspMAI	CTGCAG	5 cut(s)	19, 114, 1783, 2068, 2774
BspPI	GGATC	8 cut(s)	489, 1073, 1118, 1673, 1686, 1940, 2435, 2938
BspT107I	GGYRCC	2 cut(s)	380, 469
BspTNI	GGTCTC	1 cut(s)	1283
BsrDI	GCAATG	1 cut(s)	969
BsrFI	RCCGGY	1 cut(s)	377
BsrGI	TGTACA	1 cut(s)	976
BsrI	ACTGG	9 cut(s)	237, 596, 1099, 1798, 2281, 2337, 2389, 2787, 2862
BssAI	RCCGGY	1 cut(s)	377
BssECI	CCNNGG	6 cut(s)	661, 1906, 1929, 2019, 2571, 3141
BssNAI	GTATAC	1 cut(s)	1458
BssNI	GRCGYC	1 cut(s)	3137
BssSI	CACGAG	1 cut(s)	2108
BssT1I	CCWWGG	3 cut(s)	1906, 1929, 2019
Bst1107I	GTATAC	1 cut(s)	1458
Bst2BI	CACGAG	1 cut(s)	2108
Bst2UI	CCWGG	5 cut(s)	474, 849, 1160, 3009, 3120
Bst4CI	ACNGT	6 cut(s)	1532, 2511, 2550, 2677, 2840, 2857
Bst6I	CTCTTC	2 cut(s)	1466, 1617
BstACI	GRCGYC	1 cut(s)	3137
BstAPI	GCANNNNNTGC	2 cut(s)	2372, 3161
BstAUI	TGTACA	1 cut(s)	976
BstC8I	GCNNGC	4 cut(s)	267, 1398, 1739, 2373
BstDEI	CTNAG	9 cut(s)	579, 839, 2035, 2124, 2213, 2655, 2825, 2909, 3181
BstDSI	CCRYGG	2 cut(s)	661, 1906
BstEII	GGTNACC	3 cut(s)	1526, 3011, 3122
BstFNI	CGCG	2 cut(s)	265, 2949
BstH2I	RGCGCY	1 cut(s)	1153
BstHHI	GCGC	1 cut(s)	1152
BstMAI	GTCTC	5 cut(s)	200, 1283, 2038, 2666, 2863
BstNI	CCWGG	5 cut(s)	474, 849, 1160, 3009, 3120
BstNSI	RCATGY	4 cut(s)	129, 1022, 2375, 3159
BstPI	GGTNACC	3 cut(s)	1526, 3011, 3122
BstSCI	CCNGG	8 cut(s)	201, 472, 488, 847, 1158, 2570, 3007, 3118
BstSFI	CTRYAG	7 cut(s)	15, 110, 742, 1779, 2064, 2167, 2770
BstUI	CGCG	2 cut(s)	265, 2949
BstV1I	GCAGC	7 cut(s)	96, 397, 637, 708, 1392, 2784, 3192
BstV2I	GAAGAC	3 cut(s)	149, 2552, 2802
BstX2I	RGATCY	3 cut(s)	1678, 1756, 2943
BstXI	CCANNNNNNTGG	2 cut(s)	244, 943
BstYI	RGATCY	3 cut(s)	1678, 1756, 2943
BstZ17I	GTATAC	1 cut(s)	1458
Bsu15I	ATCGAT	1 cut(s)	816
BsuI	GTATCC	4 cut(s)	845, 2280, 2470, 2622
BsuRI	GGCC	7 cut(s)	219, 236, 248, 396, 932, 1741, 2360
BsuTUI	ATCGAT	1 cut(s)	816
BtgI	CCRYGG	2 cut(s)	661, 1906
BtgZI	GCGATG	1 cut(s)	2412
BtsI	GCAGTG	3 cut(s)	1257, 2205, 2373
BtsIMutI	CAGTG	6 cut(s)	1257, 2205, 2288, 2330, 2373, 2869
Cac8I	GCNNGC	4 cut(s)	267, 1398, 1739, 2373
CaiI	CAGNNNCTG	1 cut(s)	2936
CfoI	GCGC	1 cut(s)	1152
Cfr10I	RCCGGY	1 cut(s)	377
Cfr13I	GGNCC	2 cut(s)	199, 657
ClaI	ATCGAT	1 cut(s)	816
CseI	GACGC	1 cut(s)	3126
Csp6I	GTAC	5 cut(s)	256, 783, 977, 2297, 3087
CspCI	CAANNNNNGTGG	4 cut(s)	486, 521, 1502, 1537
CviQI	GTAC	5 cut(s)	256, 783, 977, 2297, 3087
DdeI	CTNAG	9 cut(s)	579, 839, 2035, 2124, 2213, 2655, 2825, 2909, 3181
DraI	TTTAAA	1 cut(s)	1285
EaeI	YGGCCR	6 cut(s)	217, 234, 246, 930, 1739, 2358
Eam1104I	CTCTTC	2 cut(s)	1466, 1617
EarI	CTCTTC	2 cut(s)	1466, 1617
EciI	GGCGGA	1 cut(s)	454
Eco130I	CCWWGG	3 cut(s)	1906, 1929, 2019
Eco31I	GGTCTC	1 cut(s)	1283
Eco47I	GGWCC	2 cut(s)	199, 657
Eco57I	CTGAAG	1 cut(s)	1781
Eco88I	CYCGRG	2 cut(s)	1242, 1507
Eco91I	GGTNACC	3 cut(s)	1526, 3011, 3122
EcoO109I	RGGNCCY	1 cut(s)	199
EcoO65I	GGTNACC	3 cut(s)	1526, 3011, 3122
EcoRI	GAATTC	2 cut(s)	3112, 3176
EcoRII	CCWGG	5 cut(s)	472, 847, 1158, 3007, 3118
EcoT14I	CCWWGG	3 cut(s)	1906, 1929, 2019
EcoT22I	ATGCAT	1 cut(s)	1651
ErhI	CCWWGG	3 cut(s)	1906, 1929, 2019
Esp3I	CGTCTC	2 cut(s)	2038, 2863
FalI	AAGNNNNNCTT	6 cut(s)	1265, 1297, 2042, 2074, 2411, 2443
FaqI	GGGAC	4 cut(s)	212, 602, 643, 1105
FauI	CCCGC	1 cut(s)	1135
FblI	GTMKAC	2 cut(s)	1457, 1984
Fnu4HI	GCNGC	9 cut(s)	110, 365, 411, 626, 722, 1381, 2358, 2773, 3206
Fsp4HI	GCNGC	9 cut(s)	110, 365, 411, 626, 722, 1381, 2358, 2773, 3206
FspBI	CTAG	7 cut(s)	939, 1326, 1611, 1641, 1838, 3062, 3173
GlaI	GCGC	1 cut(s)	1151
GluI	GCNGC	9 cut(s)	110, 365, 411, 626, 722, 1381, 2358, 2773, 3206
GsaI	CCCAGC	1 cut(s)	1118
GsuI	CTGGAG	2 cut(s)	2804, 2845
HaeII	RGCGCY	1 cut(s)	1153
HaeIII	GGCC	7 cut(s)	219, 236, 248, 396, 932, 1741, 2360
HapII	CCGG	6 cut(s)	203, 378, 450, 489, 2258, 2571
HgaI	GACGC	1 cut(s)	3126
HhaI	GCGC	1 cut(s)	1152
Hin1I	GRCGYC	1 cut(s)	3137
Hin6I	GCGC	1 cut(s)	1150
HinP1I	GCGC	1 cut(s)	1150
HincII	GTYRAC	1 cut(s)	882
HindII	GTYRAC	1 cut(s)	882
HindIII	AAGCTT	4 cut(s)	87, 521, 1433, 1994
HinfI	GANTC	6 cut(s)	208, 1894, 2336, 2651, 3037, 3044
HpaI	GTTAAC	1 cut(s)	882
HpaII	CCGG	6 cut(s)	203, 378, 450, 489, 2258, 2571
HphI	GGTGA	3 cut(s)	929, 971, 1520
Hpy166II	GTNNAC	5 cut(s)	882, 1458, 1985, 2578, 2696
Hpy8I	GTNNAC	5 cut(s)	882, 1458, 1985, 2578, 2696
Hpy99I	CGWCG	1 cut(s)	636
HpyCH4III	ACNGT	6 cut(s)	1532, 2511, 2550, 2677, 2840, 2857
HpyCH4IV	ACGT	3 cut(s)	1231, 1912, 2557
HpyF3I	CTNAG	9 cut(s)	579, 839, 2035, 2124, 2213, 2655, 2825, 2909, 3181
HpySE526I	ACGT	3 cut(s)	1231, 1912, 2557
Hsp92I	GRCGYC	1 cut(s)	3137
HspAI	GCGC	1 cut(s)	1150
KflI	GGGWCCC	1 cut(s)	199
Kpn2I	TCCGGA	1 cut(s)	2257
KspAI	GTTAAC	1 cut(s)	882
LmnI	GCTCC	4 cut(s)	115, 609, 633, 3218
Lsp1109I	GCAGC	7 cut(s)	96, 397, 637, 708, 1392, 2784, 3192
MaeI	CTAG	7 cut(s)	939, 1326, 1611, 1641, 1838, 3062, 3173
MaeII	ACGT	3 cut(s)	1231, 1912, 2557
MflI	RGATCY	3 cut(s)	1678, 1756, 2943
MhlI	GDGCHC	1 cut(s)	614
MlsI	TGGCCA	4 cut(s)	236, 248, 932, 1741
MluNI	TGGCCA	4 cut(s)	236, 248, 932, 1741
MlyI	GAGTC	2 cut(s)	202, 2660
MmeI	TCCRAC	2 cut(s)	1799, 3072
Mox20I	TGGCCA	4 cut(s)	236, 248, 932, 1741
Mph1103I	ATGCAT	1 cut(s)	1651
MroI	TCCGGA	1 cut(s)	2257
MroXI	GAANNNNTTC	2 cut(s)	1005, 3036
MscI	TGGCCA	4 cut(s)	236, 248, 932, 1741
MslI	CAYNNNNRTG	7 cut(s)	264, 467, 803, 941, 984, 1008, 2366
Msp20I	TGGCCA	4 cut(s)	236, 248, 932, 1741
MspA1I	CMGCKG	1 cut(s)	1383
MspI	CCGG	6 cut(s)	203, 378, 450, 489, 2258, 2571
MspR9I	CCNGG	8 cut(s)	203, 474, 490, 849, 1160, 2572, 3009, 3120
Mva1269I	GAATGC	4 cut(s)	166, 1230, 2200, 3071
MvaI	CCWGG	5 cut(s)	474, 849, 1160, 3009, 3120
MvnI	CGCG	2 cut(s)	265, 2949
NciI	CCSGG	3 cut(s)	203, 490, 2572
NcoI	CCATGG	1 cut(s)	1906
NlaIV	GGNNCC	9 cut(s)	200, 201, 382, 471, 659, 846, 1347, 1680, 2916
NmeAIII	GCCGAG	1 cut(s)	296
NmuCI	GTSAC	6 cut(s)	373, 700, 1252, 1526, 2328, 2536
NsiI	ATGCAT	1 cut(s)	1651
NspI	RCATGY	4 cut(s)	129, 1022, 2375, 3159
OliI	CACNNNNGTG	2 cut(s)	941, 2366
PaeI	GCATGC	1 cut(s)	2375
PaeR7I	CTCGAG	1 cut(s)	1507
PcsI	WCGNNNNNNNCGW	1 cut(s)	2719
PctI	GAATGC	4 cut(s)	166, 1230, 2200, 3071
PdmI	GAANNNNTTC	2 cut(s)	1005, 3036
PfeI	GAWTC	4 cut(s)	1894, 2336, 3037, 3044
PflMI	CCANNNNNTGG	2 cut(s)	1159, 2782
PfoI	TCCNGGA	1 cut(s)	847
PkrI	GCNGC	9 cut(s)	111, 366, 412, 627, 723, 1382, 2359, 2774, 3207
PleI	GAGTC	2 cut(s)	202, 2659
PpsI	GAGTC	2 cut(s)	202, 2659
PpuMI	RGGWCCY	1 cut(s)	199
PshBI	ATTAAT	2 cut(s)	1670, 2139
Psp5II	RGGWCCY	1 cut(s)	199
Psp6I	CCWGG	5 cut(s)	472, 847, 1158, 3007, 3118
PspEI	GGTNACC	3 cut(s)	1526, 3011, 3122
PspFI	CCCAGC	1 cut(s)	1114
PspGI	CCWGG	5 cut(s)	472, 847, 1158, 3007, 3118
PspN4I	GGNNCC	9 cut(s)	200, 201, 382, 471, 659, 846, 1347, 1680, 2916
PspPI	GGNCC	2 cut(s)	199, 657
PspPPI	RGGWCCY	1 cut(s)	199
PsrI	GAACNNNNNNTAC	2 cut(s)	2490, 2522
PstI	CTGCAG	5 cut(s)	19, 114, 1783, 2068, 2774
PstNI	CAGNNNCTG	1 cut(s)	2936
PsuI	RGATCY	3 cut(s)	1678, 1756, 2943
RsaI	GTAC	5 cut(s)	257, 784, 978, 2298, 3088
RsaNI	GTAC	5 cut(s)	256, 783, 977, 2297, 3087
RseI	CAYNNNNRTG	7 cut(s)	264, 467, 803, 941, 984, 1008, 2366
SatI	GCNGC	9 cut(s)	110, 365, 411, 626, 722, 1381, 2358, 2773, 3206
Sau96I	GGNCC	2 cut(s)	199, 657
SbfI	CCTGCAGG	1 cut(s)	2068
ScaI	AGTACT	1 cut(s)	3088
SchI	GAGTC	2 cut(s)	202, 2660
ScrFI	CCNGG	8 cut(s)	203, 474, 490, 849, 1160, 2572, 3009, 3120
SdaI	CCTGCAGG	1 cut(s)	2068
SduI	GDGCHC	1 cut(s)	614
SfcI	CTRYAG	7 cut(s)	15, 110, 742, 1779, 2064, 2167, 2770
Sfr274I	CTCGAG	1 cut(s)	1507
SinI	GGWCC	2 cut(s)	199, 657
SlaI	CTCGAG	1 cut(s)	1507
SmiMI	CAYNNNNRTG	7 cut(s)	264, 467, 803, 941, 984, 1008, 2366
SmlI	CTYRAG	5 cut(s)	1436, 1481, 1507, 1629, 2245
SmoI	CTYRAG	5 cut(s)	1436, 1481, 1507, 1629, 2245
SphI	GCATGC	1 cut(s)	2375
Sse8387I	CCTGCAGG	1 cut(s)	2068
SsiI	CCGC	9 cut(s)	263, 362, 365, 439, 1142, 1383, 2357, 2364, 2947
SspMI	CTAG	7 cut(s)	939, 1326, 1611, 1641, 1838, 3062, 3173
StyD4I	CCNGG	8 cut(s)	201, 472, 488, 847, 1158, 2570, 3007, 3118
StyI	CCWWGG	3 cut(s)	1906, 1929, 2019
TaaI	ACNGT	6 cut(s)	1532, 2511, 2550, 2677, 2840, 2857
TaiI	ACGT	3 cut(s)	1234, 1915, 2560
TaqI	TCGA	4 cut(s)	816, 1508, 2743, 2779
TaqII	GACCGA	1 cut(s)	1611
TatI	WGTACW	2 cut(s)	976, 3086
TauI	GCSGC	2 cut(s)	367, 2360
TfiI	GAWTC	4 cut(s)	1894, 2336, 3037, 3044
TscAI	CASTG	6 cut(s)	1257, 2205, 2288, 2337, 2373, 2869
TseFI	GTSAC	6 cut(s)	373, 700, 1252, 1526, 2328, 2536
TseI	GCWGC	7 cut(s)	109, 410, 625, 721, 1380, 2772, 3205
Tsp45I	GTSAC	6 cut(s)	373, 700, 1252, 1526, 2328, 2536
TspGWI	ACGGA	7 cut(s)	620, 1196, 1203, 1717, 2002, 2087, 2681
TspRI	CASTG	6 cut(s)	1257, 2205, 2288, 2337, 2373, 2869
Van91I	CCANNNNNTGG	2 cut(s)	1159, 2782
VpaK11BI	GGWCC	2 cut(s)	199, 657
VspI	ATTAAT	2 cut(s)	1670, 2139
XbaI	TCTAGA	2 cut(s)	1325, 3172
XceI	RCATGY	4 cut(s)	129, 1022, 2375, 3159
XcmI	CCANNNNNNNNNTGG	2 cut(s)	940, 1034
XhoI	CTCGAG	1 cut(s)	1507
XmiI	GTMKAC	2 cut(s)	1457, 1984
XmnI	GAANNNNTTC	2 cut(s)	1005, 3036
XspI	CTAG	7 cut(s)	939, 1326, 1611, 1641, 1838, 3062, 3173
ZrmI	AGTACT	1 cut(s)	3088
Zsp2I	ATGCAT	1 cut(s)	1651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.