Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 2151 |
| Acc16I | TGCGCA | 2 cut(s) | 782, 3054 |
| Acc65I | GGTACC | 3 cut(s) | 380, 1368, 3012 |
| AccB1I | GGYRCC | 4 cut(s) | 380, 469, 1368, 3012 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 1088, 1387, 2782 |
| AccI | GTMKAC | 1 cut(s) | 1457 |
| AccII | CGCG | 1 cut(s) | 1597 |
| AclWI | GGATC | 8 cut(s) | 489, 687, 1073, 1118, 1673, 1686, 1940, 2763 |
| AcoI | YGGCCR | 2 cut(s) | 930, 1739 |
| AcsI | RAATTY | 6 cut(s) | 569, 1068, 1085, 1214, 1317, 2008 |
| AcuI | CTGAAG | 6 cut(s) | 95, 1278, 1764, 1773, 2553, 2930 |
| AcyI | GRCGYC | 1 cut(s) | 2148 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 904, 1037, 1088, 1387, 2570, 2782 |
| AjnI | CCWGG | 4 cut(s) | 472, 847, 1200, 3007 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 939, 971 |
| AleI | CACNNNNGTG | 2 cut(s) | 941, 2366 |
| Alw21I | GWGCWC | 3 cut(s) | 614, 981, 3007 |
| Alw26I | GTCTC | 5 cut(s) | 9, 200, 1718, 2469, 2679 |
| Alw44I | GTGCAC | 1 cut(s) | 977 |
| AlwI | GGATC | 8 cut(s) | 489, 687, 1073, 1118, 1673, 1686, 1940, 2763 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1256, 2363, 2936 |
| Ama87I | CYCGRG | 1 cut(s) | 1242 |
| AoxI | GGCC | 5 cut(s) | 394, 930, 1276, 1739, 2307 |
| ApaLI | GTGCAC | 1 cut(s) | 977 |
| ApeKI | GCWGC | 5 cut(s) | 410, 1409, 2357, 2554, 2772 |
| ApoI | RAATTY | 6 cut(s) | 569, 1068, 1085, 1214, 1317, 2008 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2438, 2470 |
| AseI | ATTAAT | 1 cut(s) | 1670 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1488, 2235 |
| Asp718I | GGTACC | 3 cut(s) | 380, 1368, 3012 |
| AspLEI | GCGC | 6 cut(s) | 633, 783, 1152, 1311, 1597, 3055 |
| AspS9I | GGNCC | 3 cut(s) | 199, 657, 1276 |
| AsuC2I | CCSGG | 2 cut(s) | 9, 490 |
| AsuHPI | GGTGA | 4 cut(s) | 929, 971, 1520, 1963 |
| AsuNHI | GCTAGC | 1 cut(s) | 3061 |
| AvaI | CYCGRG | 1 cut(s) | 1242 |
| AvaII | GGWCC | 2 cut(s) | 199, 657 |
| BaeGI | GKGCMC | 1 cut(s) | 981 |
| BalI | TGGCCA | 2 cut(s) | 932, 1741 |
| BamHI | GGATCC | 1 cut(s) | 1678 |
| BanI | GGYRCC | 4 cut(s) | 380, 469, 1368, 3012 |
| BauI | CACGAG | 1 cut(s) | 2472 |
| BbsI | GAAGAC | 1 cut(s) | 2552 |
| Bbv12I | GWGCWC | 3 cut(s) | 614, 981, 3007 |
| BbvCI | CCTCAGC | 1 cut(s) | 579 |
| BbvI | GCAGC | 5 cut(s) | 397, 1396, 2369, 2566, 2784 |
| BceAI | ACGGC | 1 cut(s) | 618 |
| BciT130I | CCWGG | 4 cut(s) | 474, 849, 1202, 3009 |
| BciVI | GTATCC | 1 cut(s) | 2280 |
| BcnI | CCSGG | 2 cut(s) | 9, 490 |
| BcoDI | GTCTC | 5 cut(s) | 9, 200, 1718, 2469, 2679 |
| BfaI | CTAG | 4 cut(s) | 650, 1326, 1641, 3062 |
| BfmI | CTRYAG | 6 cut(s) | 270, 742, 1985, 2167, 2364, 2622 |
| BfoI | RGCGCY | 1 cut(s) | 1153 |
| BfuI | GTATCC | 1 cut(s) | 2280 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1247 |
| BglII | AGATCT | 1 cut(s) | 1756 |
| BisI | GCNGC | 5 cut(s) | 411, 1410, 2358, 2555, 2773 |
| BlpI | GCTNAGC | 1 cut(s) | 2124 |
| BlsI | GCNGC | 5 cut(s) | 412, 1411, 2359, 2556, 2774 |
| BmcAI | AGTACT | 1 cut(s) | 2630 |
| Bme1390I | CCNGG | 6 cut(s) | 9, 474, 490, 849, 1202, 3009 |
| Bme18I | GGWCC | 2 cut(s) | 199, 657 |
| BmeT110I | CYCGRG | 1 cut(s) | 1242 |
| BmgT120I | GGNCC | 3 cut(s) | 199, 657, 1276 |
| BmrFI | CCNGG | 6 cut(s) | 9, 474, 490, 849, 1202, 3009 |
| BmrI | ACTGGG | 1 cut(s) | 1803 |
| BmsI | GCATC | 9 cut(s) | 313, 1012, 1296, 1396, 1582, 1891, 2005, 2080, 2344 |
| BmtI | GCTAGC | 1 cut(s) | 3065 |
| BmuI | ACTGGG | 1 cut(s) | 1803 |
| BoxI | GACNNNNGTC | 2 cut(s) | 558, 2822 |
| BpiI | GAAGAC | 1 cut(s) | 2552 |
| BpmI | CTGGAG | 2 cut(s) | 923, 2804 |
| Bpu10I | CCTNAGC | 1 cut(s) | 579 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2124 |
| BpuEI | CTTGAG | 3 cut(s) | 89, 1181, 1457 |
| BpuMI | CCSGG | 2 cut(s) | 9, 490 |
| BsaHI | GRCGYC | 1 cut(s) | 2148 |
| BsaI | GGTCTC | 1 cut(s) | 1718 |
| BsaJI | CCNNGG | 7 cut(s) | 1312, 1381, 1872, 2019, 2034, 3008, 3069 |
| BsaWI | WCCGGW | 1 cut(s) | 449 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2499, 2529 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 904, 1037, 1088, 1387, 2570, 2782 |
| Bse1I | ACTGG | 9 cut(s) | 382, 1099, 1798, 1949, 2281, 2337, 2389, 2636, 2787 |
| Bse3DI | GCAATG | 1 cut(s) | 1864 |
| BseBI | CCWGG | 4 cut(s) | 474, 849, 1202, 3009 |
| BseDI | CCNNGG | 7 cut(s) | 1312, 1381, 1872, 2019, 2034, 3008, 3069 |
| BseGI | GGATG | 8 cut(s) | 237, 1023, 1027, 1443, 2020, 2233, 2518, 2615 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 904, 1037, 1088, 1387, 2570, 2782 |
| BseMI | GCAATG | 1 cut(s) | 1864 |
| BseMII | CTCAG | 5 cut(s) | 570, 2138, 2204, 2646, 3021 |
| BseNI | ACTGG | 9 cut(s) | 382, 1099, 1798, 1949, 2281, 2337, 2389, 2636, 2787 |
| BseSI | GKGCMC | 1 cut(s) | 981 |
| BseXI | GCAGC | 5 cut(s) | 397, 1396, 2369, 2566, 2784 |
| BseYI | CCCAGC | 1 cut(s) | 1114 |
| BsgI | GTGCAG | 1 cut(s) | 2106 |
| Bsh1236I | CGCG | 1 cut(s) | 1597 |
| BshFI | GGCC | 5 cut(s) | 396, 932, 1278, 1741, 2309 |
| BshNI | GGYRCC | 4 cut(s) | 380, 469, 1368, 3012 |
| BsiHKAI | GWGCWC | 3 cut(s) | 614, 981, 3007 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1242 |
| BsiSI | CCGG | 4 cut(s) | 9, 450, 489, 1571 |
| BsiWI | CGTACG | 1 cut(s) | 2340 |
| BslFI | GGGAC | 2 cut(s) | 643, 1105 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 904, 1037, 1088, 1387, 2570, 2782 |
| BsmAI | GTCTC | 5 cut(s) | 9, 200, 1718, 2469, 2679 |
| BsmFI | GGGAC | 2 cut(s) | 643, 1105 |
| BsmI | GAATGC | 3 cut(s) | 876, 1230, 2200 |
| BsnI | GGCC | 5 cut(s) | 396, 932, 1278, 1741, 2309 |
| Bso31I | GGTCTC | 1 cut(s) | 1718 |
| BsoBI | CYCGRG | 1 cut(s) | 1242 |
| Bsp1286I | GDGCHC | 3 cut(s) | 614, 981, 3007 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2124 |
| Bsp19I | CCATGG | 1 cut(s) | 1381 |
| BspANI | GGCC | 5 cut(s) | 396, 932, 1278, 1741, 2309 |
| BspCNI | CTCAG | 5 cut(s) | 571, 2137, 2205, 2647, 3022 |
| BspFNI | CGCG | 1 cut(s) | 1597 |
| BspMAI | CTGCAG | 1 cut(s) | 2368 |
| BspOI | GCTAGC | 1 cut(s) | 3065 |
| BspPI | GGATC | 8 cut(s) | 489, 687, 1073, 1118, 1673, 1686, 1940, 2763 |
| BspT107I | GGYRCC | 4 cut(s) | 380, 469, 1368, 3012 |
| BspTNI | GGTCTC | 1 cut(s) | 1718 |
| BsrDI | GCAATG | 1 cut(s) | 1864 |
| BsrI | ACTGG | 9 cut(s) | 382, 1099, 1798, 1949, 2281, 2337, 2389, 2636, 2787 |
| BssECI | CCNNGG | 7 cut(s) | 1312, 1381, 1872, 2019, 2034, 3008, 3069 |
| BssNAI | GTATAC | 1 cut(s) | 1458 |
| BssNI | GRCGYC | 1 cut(s) | 2148 |
| BssSI | CACGAG | 1 cut(s) | 2472 |
| BssT1I | CCWWGG | 5 cut(s) | 1312, 1381, 1872, 2019, 3069 |
| Bst1107I | GTATAC | 1 cut(s) | 1458 |
| Bst2BI | CACGAG | 1 cut(s) | 2472 |
| Bst2UI | CCWGG | 4 cut(s) | 474, 849, 1202, 3009 |
| Bst4CI | ACNGT | 5 cut(s) | 1455, 1532, 2511, 2550, 2821 |
| Bst6I | CTCTTC | 5 cut(s) | 12, 1286, 1466, 1617, 1721 |
| BstACI | GRCGYC | 1 cut(s) | 2148 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2363, 2372 |
| BstC8I | GCNNGC | 4 cut(s) | 1398, 1739, 2373, 3063 |
| BstDEI | CTNAG | 6 cut(s) | 141, 579, 2124, 2213, 2655, 3030 |
| BstDSI | CCRYGG | 1 cut(s) | 1381 |
| BstEII | GGTNACC | 1 cut(s) | 1526 |
| BstF5I | GGATG | 8 cut(s) | 237, 1023, 1027, 1443, 2020, 2233, 2518, 2615 |
| BstFNI | CGCG | 1 cut(s) | 1597 |
| BstH2I | RGCGCY | 1 cut(s) | 1153 |
| BstHHI | GCGC | 6 cut(s) | 633, 783, 1152, 1311, 1597, 3055 |
| BstMAI | GTCTC | 5 cut(s) | 9, 200, 1718, 2469, 2679 |
| BstNI | CCWGG | 4 cut(s) | 474, 849, 1202, 3009 |
| BstNSI | RCATGY | 2 cut(s) | 1022, 2375 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 558, 2822 |
| BstPI | GGTNACC | 1 cut(s) | 1526 |
| BstSCI | CCNGG | 6 cut(s) | 7, 472, 488, 847, 1200, 3007 |
| BstSFI | CTRYAG | 6 cut(s) | 270, 742, 1985, 2167, 2364, 2622 |
| BstSLI | GKGCMC | 1 cut(s) | 981 |
| BstUI | CGCG | 1 cut(s) | 1597 |
| BstV1I | GCAGC | 5 cut(s) | 397, 1396, 2369, 2566, 2784 |
| BstV2I | GAAGAC | 1 cut(s) | 2552 |
| BstX2I | RGATCY | 2 cut(s) | 1678, 1756 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 943, 1208 |
| BstYI | RGATCY | 2 cut(s) | 1678, 1756 |
| BstZ17I | GTATAC | 1 cut(s) | 1458 |
| BsuI | GTATCC | 1 cut(s) | 2280 |
| BsuRI | GGCC | 5 cut(s) | 396, 932, 1278, 1741, 2309 |
| BtgI | CCRYGG | 1 cut(s) | 1381 |
| BtgZI | GCGATG | 1 cut(s) | 1612 |
| BtsCI | GGATG | 8 cut(s) | 237, 1023, 1027, 1443, 2020, 2233, 2518, 2615 |
| BtsI | GCAGTG | 5 cut(s) | 1257, 2093, 2361, 2373, 2846 |
| BtsIMutI | CAGTG | 8 cut(s) | 1257, 1749, 2093, 2288, 2330, 2361, 2373, 2846 |
| Cac8I | GCNNGC | 4 cut(s) | 1398, 1739, 2373, 3063 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1256, 2363, 2936 |
| CfoI | GCGC | 6 cut(s) | 633, 783, 1152, 1311, 1597, 3055 |
| Cfr13I | GGNCC | 3 cut(s) | 199, 657, 1276 |
| CseI | GACGC | 1 cut(s) | 2546 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1502, 1537 |
| DdeI | CTNAG | 6 cut(s) | 141, 579, 2124, 2213, 2655, 3030 |
| DraI | TTTAAA | 1 cut(s) | 93 |
| EaeI | YGGCCR | 2 cut(s) | 930, 1739 |
| Eam1104I | CTCTTC | 5 cut(s) | 12, 1286, 1466, 1617, 1721 |
| EarI | CTCTTC | 5 cut(s) | 12, 1286, 1466, 1617, 1721 |
| Eco130I | CCWWGG | 5 cut(s) | 1312, 1381, 1872, 2019, 3069 |
| Eco31I | GGTCTC | 1 cut(s) | 1718 |
| Eco47I | GGWCC | 2 cut(s) | 199, 657 |
| Eco57I | CTGAAG | 6 cut(s) | 95, 1278, 1764, 1773, 2553, 2930 |
| Eco88I | CYCGRG | 1 cut(s) | 1242 |
| Eco91I | GGTNACC | 1 cut(s) | 1526 |
| EcoO109I | RGGNCCY | 2 cut(s) | 199, 1276 |
| EcoO65I | GGTNACC | 1 cut(s) | 1526 |
| EcoRII | CCWGG | 4 cut(s) | 472, 847, 1200, 3007 |
| EcoT14I | CCWWGG | 5 cut(s) | 1312, 1381, 1872, 2019, 3069 |
| ErhI | CCWWGG | 5 cut(s) | 1312, 1381, 1872, 2019, 3069 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1265, 1297, 2042, 2074, 2411, 2443 |
| FaqI | GGGAC | 2 cut(s) | 643, 1105 |
| FauNDI | CATATG | 1 cut(s) | 1651 |
| FblI | GTMKAC | 1 cut(s) | 1457 |
| Fnu4HI | GCNGC | 5 cut(s) | 411, 1410, 2358, 2555, 2773 |
| FokI | GGATG | 8 cut(s) | 244, 1010, 1014, 1430, 2027, 2240, 2505, 2602 |
| Fsp4HI | GCNGC | 5 cut(s) | 411, 1410, 2358, 2555, 2773 |
| FspAI | RTGCGCAY | 1 cut(s) | 3054 |
| FspBI | CTAG | 4 cut(s) | 650, 1326, 1641, 3062 |
| FspI | TGCGCA | 2 cut(s) | 782, 3054 |
| GlaI | GCGC | 6 cut(s) | 632, 782, 1151, 1310, 1596, 3054 |
| GluI | GCNGC | 5 cut(s) | 411, 1410, 2358, 2555, 2773 |
| GsaI | CCCAGC | 1 cut(s) | 1118 |
| GsuI | CTGGAG | 2 cut(s) | 923, 2804 |
| HaeII | RGCGCY | 1 cut(s) | 1153 |
| HaeIII | GGCC | 5 cut(s) | 396, 932, 1278, 1741, 2309 |
| HapII | CCGG | 4 cut(s) | 9, 450, 489, 1571 |
| HgaI | GACGC | 1 cut(s) | 2546 |
| HhaI | GCGC | 6 cut(s) | 633, 783, 1152, 1311, 1597, 3055 |
| Hin1I | GRCGYC | 1 cut(s) | 2148 |
| Hin6I | GCGC | 6 cut(s) | 631, 781, 1150, 1309, 1595, 3053 |
| HinP1I | GCGC | 6 cut(s) | 631, 781, 1150, 1309, 1595, 3053 |
| HindIII | AAGCTT | 5 cut(s) | 87, 107, 521, 1433, 1994 |
| HpaII | CCGG | 4 cut(s) | 9, 450, 489, 1571 |
| HphI | GGTGA | 4 cut(s) | 929, 971, 1520, 1963 |
| Hpy166II | GTNNAC | 3 cut(s) | 979, 1458, 2696 |
| Hpy8I | GTNNAC | 3 cut(s) | 979, 1458, 2696 |
| HpyAV | CCTTC | 8 cut(s) | 40, 395, 1038, 1214, 1267, 2036, 2253, 2905 |
| HpyCH4III | ACNGT | 5 cut(s) | 1455, 1532, 2511, 2550, 2821 |
| HpyCH4IV | ACGT | 2 cut(s) | 1231, 2148 |
| HpyF3I | CTNAG | 6 cut(s) | 141, 579, 2124, 2213, 2655, 3030 |
| HpySE526I | ACGT | 2 cut(s) | 1231, 2148 |
| Hsp92I | GRCGYC | 1 cut(s) | 2148 |
| HspAI | GCGC | 6 cut(s) | 631, 781, 1150, 1309, 1595, 3053 |
| KpnI | GGTACC | 3 cut(s) | 384, 1372, 3016 |
| LmnI | GCTCC | 5 cut(s) | 115, 338, 609, 2520, 3010 |
| Lsp1109I | GCAGC | 5 cut(s) | 397, 1396, 2369, 2566, 2784 |
| LweI | GCATC | 9 cut(s) | 313, 1012, 1296, 1396, 1582, 1891, 2005, 2080, 2344 |
| MaeI | CTAG | 4 cut(s) | 650, 1326, 1641, 3062 |
| MaeII | ACGT | 2 cut(s) | 1231, 2148 |
| MflI | RGATCY | 2 cut(s) | 1678, 1756 |
| MhlI | GDGCHC | 3 cut(s) | 614, 981, 3007 |
| MlsI | TGGCCA | 2 cut(s) | 932, 1741 |
| MluNI | TGGCCA | 2 cut(s) | 932, 1741 |
| MlyI | GAGTC | 4 cut(s) | 202, 2584, 2660, 2681 |
| MmeI | TCCRAC | 2 cut(s) | 1123, 1799 |
| Mox20I | TGGCCA | 2 cut(s) | 932, 1741 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1488, 2235 |
| MscI | TGGCCA | 2 cut(s) | 932, 1741 |
| MseI | TTAA | 7 cut(s) | 92, 542, 1137, 1170, 1374, 1670, 3106 |
| MslI | CAYNNNNRTG | 5 cut(s) | 803, 941, 984, 1008, 2366 |
| Msp20I | TGGCCA | 2 cut(s) | 932, 1741 |
| MspI | CCGG | 4 cut(s) | 9, 450, 489, 1571 |
| MspR9I | CCNGG | 6 cut(s) | 9, 474, 490, 849, 1202, 3009 |
| Mva1269I | GAATGC | 3 cut(s) | 876, 1230, 2200 |
| MvaI | CCWGG | 4 cut(s) | 474, 849, 1202, 3009 |
| MvnI | CGCG | 1 cut(s) | 1597 |
| NciI | CCSGG | 2 cut(s) | 9, 490 |
| NcoI | CCATGG | 1 cut(s) | 1381 |
| NdeI | CATATG | 1 cut(s) | 1651 |
| NheI | GCTAGC | 1 cut(s) | 3061 |
| NmuCI | GTSAC | 8 cut(s) | 373, 700, 1252, 1526, 1969, 2328, 2536, 2576 |
| NsbI | TGCGCA | 2 cut(s) | 782, 3054 |
| NspI | RCATGY | 2 cut(s) | 1022, 2375 |
| OliI | CACNNNNGTG | 2 cut(s) | 941, 2366 |
| PaeI | GCATGC | 1 cut(s) | 2375 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2719 |
| PctI | GAATGC | 3 cut(s) | 876, 1230, 2200 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1488, 2235 |
| PfeI | GAWTC | 6 cut(s) | 1048, 1894, 2336, 2858, 3037, 3044 |
| Pfl23II | CGTACG | 1 cut(s) | 2340 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 1088, 1387, 2782 |
| PfoI | TCCNGGA | 2 cut(s) | 7, 847 |
| PkrI | GCNGC | 5 cut(s) | 412, 1411, 2359, 2556, 2774 |
| PleI | GAGTC | 4 cut(s) | 202, 2583, 2659, 2680 |
| PpsI | GAGTC | 4 cut(s) | 202, 2583, 2659, 2680 |
| PpuMI | RGGWCCY | 1 cut(s) | 199 |
| PshAI | GACNNNNGTC | 2 cut(s) | 558, 2822 |
| PshBI | ATTAAT | 1 cut(s) | 1670 |
| Psp5II | RGGWCCY | 1 cut(s) | 199 |
| Psp6I | CCWGG | 4 cut(s) | 472, 847, 1200, 3007 |
| PspEI | GGTNACC | 1 cut(s) | 1526 |
| PspFI | CCCAGC | 1 cut(s) | 1114 |
| PspGI | CCWGG | 4 cut(s) | 472, 847, 1200, 3007 |
| PspLI | CGTACG | 1 cut(s) | 2340 |
| PspPI | GGNCC | 3 cut(s) | 199, 657, 1276 |
| PspPPI | RGGWCCY | 1 cut(s) | 199 |
| PstI | CTGCAG | 1 cut(s) | 2368 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1256, 2363, 2936 |
| PsuI | RGATCY | 2 cut(s) | 1678, 1756 |
| RseI | CAYNNNNRTG | 5 cut(s) | 803, 941, 984, 1008, 2366 |
| SaqAI | TTAA | 7 cut(s) | 92, 542, 1137, 1170, 1374, 1670, 3106 |
| SatI | GCNGC | 5 cut(s) | 411, 1410, 2358, 2555, 2773 |
| Sau96I | GGNCC | 3 cut(s) | 199, 657, 1276 |
| ScaI | AGTACT | 1 cut(s) | 2630 |
| SchI | GAGTC | 4 cut(s) | 202, 2584, 2660, 2681 |
| ScrFI | CCNGG | 6 cut(s) | 9, 474, 490, 849, 1202, 3009 |
| SduI | GDGCHC | 3 cut(s) | 614, 981, 3007 |
| SfaNI | GCATC | 9 cut(s) | 313, 1012, 1296, 1396, 1582, 1891, 2005, 2080, 2344 |
| SfcI | CTRYAG | 6 cut(s) | 270, 742, 1985, 2167, 2364, 2622 |
| SinI | GGWCC | 2 cut(s) | 199, 657 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 803, 941, 984, 1008, 2366 |
| SmlI | CTYRAG | 3 cut(s) | 104, 1160, 1436 |
| SmoI | CTYRAG | 3 cut(s) | 104, 1160, 1436 |
| SphI | GCATGC | 1 cut(s) | 2375 |
| SspI | AATATT | 1 cut(s) | 1577 |
| SspMI | CTAG | 4 cut(s) | 650, 1326, 1641, 3062 |
| StyD4I | CCNGG | 6 cut(s) | 7, 472, 488, 847, 1200, 3007 |
| StyI | CCWWGG | 5 cut(s) | 1312, 1381, 1872, 2019, 3069 |
| TaaI | ACNGT | 5 cut(s) | 1455, 1532, 2511, 2550, 2821 |
| TaiI | ACGT | 2 cut(s) | 1234, 2151 |
| TaqI | TCGA | 3 cut(s) | 433, 1508, 1922 |
| TatI | WGTACW | 2 cut(s) | 134, 2628 |
| TfiI | GAWTC | 6 cut(s) | 1048, 1894, 2336, 2858, 3037, 3044 |
| Tru1I | TTAA | 7 cut(s) | 92, 542, 1137, 1170, 1374, 1670, 3106 |
| Tru9I | TTAA | 7 cut(s) | 92, 542, 1137, 1170, 1374, 1670, 3106 |
| TscAI | CASTG | 8 cut(s) | 1257, 1756, 2100, 2288, 2337, 2368, 2373, 2853 |
| TseFI | GTSAC | 8 cut(s) | 373, 700, 1252, 1526, 1969, 2328, 2536, 2576 |
| TseI | GCWGC | 5 cut(s) | 410, 1409, 2357, 2554, 2772 |
| Tsp45I | GTSAC | 8 cut(s) | 373, 700, 1252, 1526, 1969, 2328, 2536, 2576 |
| TspGWI | ACGGA | 4 cut(s) | 244, 1203, 2511, 2681 |
| TspRI | CASTG | 8 cut(s) | 1257, 1756, 2100, 2288, 2337, 2368, 2373, 2853 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 1088, 1387, 2782 |
| VneI | GTGCAC | 1 cut(s) | 977 |
| VpaK11BI | GGWCC | 2 cut(s) | 199, 657 |
| VspI | ATTAAT | 1 cut(s) | 1670 |
| XapI | RAATTY | 6 cut(s) | 569, 1068, 1085, 1214, 1317, 2008 |
| XbaI | TCTAGA | 1 cut(s) | 1325 |
| XceI | RCATGY | 2 cut(s) | 1022, 2375 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 940, 1034 |
| XmiI | GTMKAC | 1 cut(s) | 1457 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1488, 2235 |
| XspI | CTAG | 4 cut(s) | 650, 1326, 1641, 3062 |
| ZraI | GACGTC | 1 cut(s) | 2149 |
| ZrmI | AGTACT | 1 cut(s) | 2630 |