Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 94, 328 |
| AccII | CGCG | 1 cut(s) | 873 |
| AccIII | TCCGGA | 1 cut(s) | 1059 |
| AciI | CCGC | 3 cut(s) | 197, 208, 350 |
| AclWI | GGATC | 2 cut(s) | 545, 1051 |
| AdeI | CACNNNGTG | 1 cut(s) | 594 |
| AfaI | GTAC | 3 cut(s) | 227, 497, 722 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 191, 680, 1150, 1192 |
| AgsI | TTSAA | 6 cut(s) | 163, 1052, 1073, 1114, 1211, 1223 |
| AjnI | CCWGG | 4 cut(s) | 679, 813, 876, 1036 |
| AluBI | AGCT | 6 cut(s) | 502, 741, 763, 1000, 1018, 1177 |
| AluI | AGCT | 6 cut(s) | 502, 741, 763, 1000, 1018, 1177 |
| Alw26I | GTCTC | 5 cut(s) | 515, 777, 801, 826, 1209 |
| AlwI | GGATC | 2 cut(s) | 545, 1051 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 473, 983 |
| Ama87I | CYCGRG | 1 cut(s) | 1145 |
| Aor13HI | TCCGGA | 1 cut(s) | 1059 |
| AoxI | GGCC | 2 cut(s) | 144, 613 |
| ApeKI | GCWGC | 3 cut(s) | 467, 959, 1124 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 167 |
| AspLEI | GCGC | 1 cut(s) | 873 |
| AspS9I | GGNCC | 2 cut(s) | 124, 1141 |
| AsuHPI | GGTGA | 1 cut(s) | 73 |
| AvaI | CYCGRG | 1 cut(s) | 1145 |
| AvaII | GGWCC | 2 cut(s) | 124, 1141 |
| AxyI | CCTNAGG | 1 cut(s) | 617 |
| BbsI | GAAGAC | 1 cut(s) | 505 |
| BbvI | GCAGC | 3 cut(s) | 479, 946, 1111 |
| BccI | CCATC | 2 cut(s) | 170, 387 |
| BceAI | ACGGC | 1 cut(s) | 658 |
| BciT130I | CCWGG | 4 cut(s) | 681, 815, 878, 1038 |
| BclI | TGATCA | 1 cut(s) | 729 |
| BcoDI | GTCTC | 5 cut(s) | 515, 777, 801, 826, 1209 |
| BfaI | CTAG | 2 cut(s) | 86, 969 |
| BglII | AGATCT | 1 cut(s) | 1045 |
| BisI | GCNGC | 3 cut(s) | 468, 960, 1125 |
| BlsI | GCNGC | 3 cut(s) | 469, 961, 1126 |
| Bme1390I | CCNGG | 4 cut(s) | 681, 815, 878, 1038 |
| Bme18I | GGWCC | 2 cut(s) | 124, 1141 |
| BmeT110I | CYCGRG | 1 cut(s) | 1145 |
| BmgT120I | GGNCC | 2 cut(s) | 124, 1141 |
| BmiI | GGNNCC | 2 cut(s) | 126, 1143 |
| BmrFI | CCNGG | 4 cut(s) | 681, 815, 878, 1038 |
| BmsI | GCATC | 4 cut(s) | 190, 454, 707, 1034 |
| BpiI | GAAGAC | 1 cut(s) | 505 |
| BpmI | CTGGAG | 1 cut(s) | 1020 |
| BpuEI | CTTGAG | 2 cut(s) | 998, 1208 |
| Bsa29I | ATCGAT | 1 cut(s) | 945 |
| BsaJI | CCNNGG | 4 cut(s) | 129, 680, 813, 814 |
| BsaWI | WCCGGW | 1 cut(s) | 1059 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 823, 853 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 191, 680, 1150, 1192 |
| Bse1I | ACTGG | 1 cut(s) | 906 |
| Bse21I | CCTNAGG | 1 cut(s) | 617 |
| Bse3DI | GCAATG | 2 cut(s) | 52, 553 |
| BseAI | TCCGGA | 1 cut(s) | 1059 |
| BseBI | CCWGG | 4 cut(s) | 681, 815, 878, 1038 |
| BseCI | ATCGAT | 1 cut(s) | 945 |
| BseDI | CCNNGG | 4 cut(s) | 129, 680, 813, 814 |
| BseGI | GGATG | 3 cut(s) | 205, 343, 628 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 191, 680, 1150, 1192 |
| BseMI | GCAATG | 2 cut(s) | 52, 553 |
| BseMII | CTCAG | 5 cut(s) | 177, 248, 417, 474, 608 |
| BseNI | ACTGG | 1 cut(s) | 906 |
| BseXI | GCAGC | 3 cut(s) | 479, 946, 1111 |
| BsgI | GTGCAG | 1 cut(s) | 996 |
| Bsh1236I | CGCG | 1 cut(s) | 873 |
| BshFI | GGCC | 2 cut(s) | 146, 615 |
| BshVI | ATCGAT | 1 cut(s) | 945 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1145 |
| BsiSI | CCGG | 1 cut(s) | 1060 |
| BslFI | GGGAC | 3 cut(s) | 168, 599, 1127 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 191, 680, 1150, 1192 |
| BsmAI | GTCTC | 5 cut(s) | 515, 777, 801, 826, 1209 |
| BsmFI | GGGAC | 3 cut(s) | 168, 599, 1127 |
| BsmI | GAATGC | 1 cut(s) | 310 |
| BsnI | GGCC | 2 cut(s) | 146, 615 |
| BsoBI | CYCGRG | 1 cut(s) | 1145 |
| Bsp13I | TCCGGA | 1 cut(s) | 1059 |
| Bsp143I | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| BspACI | CCGC | 3 cut(s) | 197, 208, 350 |
| BspANI | GGCC | 2 cut(s) | 146, 615 |
| BspCNI | CTCAG | 5 cut(s) | 178, 247, 416, 473, 609 |
| BspDI | ATCGAT | 1 cut(s) | 945 |
| BspEI | TCCGGA | 1 cut(s) | 1059 |
| BspFNI | CGCG | 1 cut(s) | 873 |
| BspLI | GGNNCC | 2 cut(s) | 126, 1143 |
| BspPI | GGATC | 2 cut(s) | 545, 1051 |
| BsrDI | GCAATG | 2 cut(s) | 52, 553 |
| BsrI | ACTGG | 1 cut(s) | 906 |
| BssECI | CCNNGG | 4 cut(s) | 129, 680, 813, 814 |
| BssMI | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| BssNAI | GTATAC | 1 cut(s) | 329 |
| BssT1I | CCWWGG | 1 cut(s) | 129 |
| Bst1107I | GTATAC | 1 cut(s) | 329 |
| Bst2UI | CCWGG | 4 cut(s) | 681, 815, 878, 1038 |
| Bst4CI | ACNGT | 4 cut(s) | 281, 433, 660, 940 |
| Bst6I | CTCTTC | 1 cut(s) | 1206 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 473, 670 |
| BstC8I | GCNNGC | 2 cut(s) | 918, 1122 |
| BstDEI | CTNAG | 5 cut(s) | 186, 234, 403, 460, 617 |
| BstEII | GGTNACC | 1 cut(s) | 275 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1190 |
| BstF5I | GGATG | 3 cut(s) | 205, 343, 628 |
| BstFNI | CGCG | 1 cut(s) | 873 |
| BstHHI | GCGC | 1 cut(s) | 873 |
| BstKTI | GATC | 5 cut(s) | 553, 732, 806, 1048, 1059 |
| BstMAI | GTCTC | 5 cut(s) | 515, 777, 801, 826, 1209 |
| BstMBI | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 473, 482, 499, 670 |
| BstNI | CCWGG | 4 cut(s) | 681, 815, 878, 1038 |
| BstPI | GGTNACC | 1 cut(s) | 275 |
| BstSCI | CCNGG | 4 cut(s) | 679, 813, 876, 1036 |
| BstUI | CGCG | 1 cut(s) | 873 |
| BstV1I | GCAGC | 3 cut(s) | 479, 946, 1111 |
| BstV2I | GAAGAC | 1 cut(s) | 505 |
| BstX2I | RGATCY | 2 cut(s) | 1045, 1056 |
| BstYI | RGATCY | 2 cut(s) | 1045, 1056 |
| BstZ17I | GTATAC | 1 cut(s) | 329 |
| Bsu15I | ATCGAT | 1 cut(s) | 945 |
| Bsu36I | CCTNAGG | 1 cut(s) | 617 |
| BsuRI | GGCC | 2 cut(s) | 146, 615 |
| BsuTUI | ATCGAT | 1 cut(s) | 945 |
| BtsCI | GGATG | 3 cut(s) | 205, 343, 628 |
| BtsI | GCAGTG | 3 cut(s) | 228, 315, 984 |
| BtsIMutI | CAGTG | 5 cut(s) | 228, 290, 315, 411, 984 |
| Cac8I | GCNNGC | 2 cut(s) | 918, 1122 |
| CaiI | CAGNNNCTG | 2 cut(s) | 473, 983 |
| CfoI | GCGC | 1 cut(s) | 873 |
| Cfr13I | GGNCC | 2 cut(s) | 124, 1141 |
| ClaI | ATCGAT | 1 cut(s) | 945 |
| Csp6I | GTAC | 3 cut(s) | 226, 496, 721 |
| CviAII | CATG | 4 cut(s) | 98, 637, 1022, 1091 |
| CviQI | GTAC | 3 cut(s) | 226, 496, 721 |
| DdeI | CTNAG | 5 cut(s) | 186, 234, 403, 460, 617 |
| DpnI | GATC | 5 cut(s) | 552, 731, 805, 1047, 1058 |
| DpnII | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| DraIII | CACNNNGTG | 1 cut(s) | 594 |
| Eam1104I | CTCTTC | 1 cut(s) | 1206 |
| EarI | CTCTTC | 1 cut(s) | 1206 |
| EciI | GGCGGA | 1 cut(s) | 339 |
| Eco130I | CCWWGG | 1 cut(s) | 129 |
| Eco32I | GATATC | 1 cut(s) | 259 |
| Eco47I | GGWCC | 2 cut(s) | 124, 1141 |
| Eco81I | CCTNAGG | 1 cut(s) | 617 |
| Eco88I | CYCGRG | 1 cut(s) | 1145 |
| Eco91I | GGTNACC | 1 cut(s) | 275 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1190 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1141 |
| EcoO65I | GGTNACC | 1 cut(s) | 275 |
| EcoRII | CCWGG | 4 cut(s) | 679, 813, 876, 1036 |
| EcoRV | GATATC | 1 cut(s) | 259 |
| EcoT14I | CCWWGG | 1 cut(s) | 129 |
| EcoT22I | ATGCAT | 1 cut(s) | 1027 |
| ErhI | CCWWGG | 1 cut(s) | 129 |
| FaeI | CATG | 4 cut(s) | 101, 640, 1025, 1094 |
| FaqI | GGGAC | 3 cut(s) | 168, 599, 1127 |
| FatI | CATG | 4 cut(s) | 97, 636, 1021, 1090 |
| FauNDI | CATATG | 1 cut(s) | 643 |
| FbaI | TGATCA | 1 cut(s) | 729 |
| FblI | GTMKAC | 2 cut(s) | 94, 328 |
| Fnu4HI | GCNGC | 3 cut(s) | 468, 960, 1125 |
| FokI | GGATG | 3 cut(s) | 212, 350, 615 |
| Fsp4HI | GCNGC | 3 cut(s) | 468, 960, 1125 |
| FspBI | CTAG | 2 cut(s) | 86, 969 |
| GlaI | GCGC | 1 cut(s) | 872 |
| GluI | GCNGC | 3 cut(s) | 468, 960, 1125 |
| GsuI | CTGGAG | 1 cut(s) | 1020 |
| HaeIII | GGCC | 2 cut(s) | 146, 615 |
| HapII | CCGG | 1 cut(s) | 1060 |
| HhaI | GCGC | 1 cut(s) | 873 |
| Hin1II | CATG | 4 cut(s) | 101, 640, 1025, 1094 |
| Hin6I | GCGC | 1 cut(s) | 871 |
| HinP1I | GCGC | 1 cut(s) | 871 |
| HincII | GTYRAC | 1 cut(s) | 64 |
| HindII | GTYRAC | 1 cut(s) | 64 |
| HindIII | AAGCTT | 2 cut(s) | 998, 1016 |
| HinfI | GANTC | 5 cut(s) | 397, 446, 1181, 1214, 1219 |
| HpaII | CCGG | 1 cut(s) | 1060 |
| HphI | GGTGA | 1 cut(s) | 73 |
| Hpy166II | GTNNAC | 4 cut(s) | 64, 95, 228, 329 |
| Hpy188I | TCNGA | 3 cut(s) | 241, 463, 803 |
| Hpy8I | GTNNAC | 4 cut(s) | 64, 95, 228, 329 |
| Hpy99I | CGWCG | 1 cut(s) | 679 |
| HpyAV | CCTTC | 3 cut(s) | 157, 1144, 1186 |
| HpyCH4III | ACNGT | 4 cut(s) | 281, 433, 660, 940 |
| HpyCH4IV | ACGT | 2 cut(s) | 91, 667 |
| HpyCH4V | TGCA | 7 cut(s) | 14, 308, 467, 563, 581, 977, 1025 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 473, 482, 499, 670 |
| HpyF3I | CTNAG | 5 cut(s) | 186, 234, 403, 460, 617 |
| HpySE526I | ACGT | 2 cut(s) | 91, 667 |
| Hsp92II | CATG | 4 cut(s) | 101, 640, 1025, 1094 |
| HspAI | GCGC | 1 cut(s) | 871 |
| Kpn2I | TCCGGA | 1 cut(s) | 1059 |
| Ksp22I | TGATCA | 1 cut(s) | 729 |
| Kzo9I | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| LmnI | GCTCC | 2 cut(s) | 760, 967 |
| Lsp1109I | GCAGC | 3 cut(s) | 479, 946, 1111 |
| LweI | GCATC | 4 cut(s) | 190, 454, 707, 1034 |
| MaeI | CTAG | 2 cut(s) | 86, 969 |
| MaeII | ACGT | 2 cut(s) | 91, 667 |
| MaeIII | GTNAC | 5 cut(s) | 275, 290, 621, 646, 1153 |
| MalI | GATC | 5 cut(s) | 552, 731, 805, 1047, 1058 |
| MboI | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| MboII | GAAGA | 5 cut(s) | 505, 665, 1117, 1145, 1223 |
| MfeI | CAATTG | 1 cut(s) | 158 |
| MflI | RGATCY | 2 cut(s) | 1045, 1056 |
| MluCI | AATT | 5 cut(s) | 134, 158, 705, 906, 1109 |
| MlyI | GAGTC | 1 cut(s) | 1223 |
| MmeI | TCCRAC | 1 cut(s) | 989 |
| MnlI | CCTC | 7 cut(s) | 106, 145, 364, 564, 612, 874, 1089 |
| Mph1103I | ATGCAT | 1 cut(s) | 1027 |
| MroI | TCCGGA | 1 cut(s) | 1059 |
| MroXI | GAANNNNTTC | 1 cut(s) | 167 |
| MseI | TTAA | 2 cut(s) | 909, 1252 |
| MspA1I | CMGCKG | 1 cut(s) | 502 |
| MspI | CCGG | 1 cut(s) | 1060 |
| MspR9I | CCNGG | 4 cut(s) | 681, 815, 878, 1038 |
| MunI | CAATTG | 1 cut(s) | 158 |
| Mva1269I | GAATGC | 1 cut(s) | 310 |
| MvaI | CCWGG | 4 cut(s) | 681, 815, 878, 1038 |
| MvnI | CGCG | 1 cut(s) | 873 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 473, 482, 499, 670 |
| NdeI | CATATG | 1 cut(s) | 643 |
| NdeII | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| NlaIII | CATG | 4 cut(s) | 101, 640, 1025, 1094 |
| NlaIV | GGNNCC | 2 cut(s) | 126, 1143 |
| NmuCI | GTSAC | 1 cut(s) | 646 |
| NsiI | ATGCAT | 1 cut(s) | 1027 |
| PasI | CCCWGGG | 1 cut(s) | 814 |
| PctI | GAATGC | 1 cut(s) | 310 |
| PdmI | GAANNNNTTC | 1 cut(s) | 167 |
| PfeI | GAWTC | 4 cut(s) | 397, 446, 1181, 1219 |
| PfoI | TCCNGGA | 1 cut(s) | 1036 |
| PkrI | GCNGC | 3 cut(s) | 469, 961, 1126 |
| PleI | GAGTC | 1 cut(s) | 1222 |
| PpsI | GAGTC | 1 cut(s) | 1222 |
| PpuMI | RGGWCCY | 1 cut(s) | 1141 |
| Psp5II | RGGWCCY | 1 cut(s) | 1141 |
| Psp6I | CCWGG | 4 cut(s) | 679, 813, 876, 1036 |
| PspEI | GGTNACC | 1 cut(s) | 275 |
| PspGI | CCWGG | 4 cut(s) | 679, 813, 876, 1036 |
| PspN4I | GGNNCC | 2 cut(s) | 126, 1143 |
| PspPI | GGNCC | 2 cut(s) | 124, 1141 |
| PspPPI | RGGWCCY | 1 cut(s) | 1141 |
| PstNI | CAGNNNCTG | 2 cut(s) | 473, 983 |
| PsuI | RGATCY | 2 cut(s) | 1045, 1056 |
| PvuII | CAGCTG | 1 cut(s) | 502 |
| RsaI | GTAC | 3 cut(s) | 227, 497, 722 |
| RsaNI | GTAC | 3 cut(s) | 226, 496, 721 |
| SaqAI | TTAA | 2 cut(s) | 909, 1252 |
| SatI | GCNGC | 3 cut(s) | 468, 960, 1125 |
| Sau3AI | GATC | 5 cut(s) | 550, 729, 803, 1045, 1056 |
| Sau96I | GGNCC | 2 cut(s) | 124, 1141 |
| SchI | GAGTC | 1 cut(s) | 1223 |
| ScrFI | CCNGG | 4 cut(s) | 681, 815, 878, 1038 |
| SfaNI | GCATC | 4 cut(s) | 190, 454, 707, 1034 |
| SinI | GGWCC | 2 cut(s) | 124, 1141 |
| SmlI | CTYRAG | 2 cut(s) | 1013, 1187 |
| SmoI | CTYRAG | 2 cut(s) | 1013, 1187 |
| Sse9I | AATT | 5 cut(s) | 134, 158, 705, 906, 1109 |
| SsiI | CCGC | 3 cut(s) | 197, 208, 350 |
| SspMI | CTAG | 2 cut(s) | 86, 969 |
| StyD4I | CCNGG | 4 cut(s) | 679, 813, 876, 1036 |
| StyI | CCWWGG | 1 cut(s) | 129 |
| TaaI | ACNGT | 4 cut(s) | 281, 433, 660, 940 |
| TaiI | ACGT | 2 cut(s) | 94, 670 |
| TaqI | TCGA | 4 cut(s) | 7, 945, 1103, 1217 |
| TasI | AATT | 5 cut(s) | 134, 158, 705, 906, 1109 |
| TfiI | GAWTC | 4 cut(s) | 397, 446, 1181, 1219 |
| Tru1I | TTAA | 2 cut(s) | 909, 1252 |
| Tru9I | TTAA | 2 cut(s) | 909, 1252 |
| TscAI | CASTG | 5 cut(s) | 235, 290, 315, 411, 984 |
| TseFI | GTSAC | 1 cut(s) | 646 |
| TseI | GCWGC | 3 cut(s) | 467, 959, 1124 |
| Tsp45I | GTSAC | 1 cut(s) | 646 |
| TspDTI | ATGAA | 8 cut(s) | 429, 505, 617, 625, 765, 1008, 1010, 1146 |
| TspRI | CASTG | 5 cut(s) | 235, 290, 315, 411, 984 |
| VpaK11BI | GGWCC | 2 cut(s) | 124, 1141 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1190 |
| XmiI | GTMKAC | 2 cut(s) | 94, 328 |
| XmnI | GAANNNNTTC | 1 cut(s) | 167 |
| XspI | CTAG | 2 cut(s) | 86, 969 |
| Zsp2I | ATGCAT | 1 cut(s) | 1027 |