Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0000332

Physical Location & Seq

Genomic Coordinates Forward (+)

57097128 .. 57099766

Gene Structure 2639 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG425000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGGTGAACTGGGATTGTGTGTTGCGGACCGCAATATCTGGTATTGAGGTGGATCCTCCAATTGAAATAAAAGAAAGGACACTACTAGAAGTTTCTGGATATGAGAACCCTGTCGAATTTGGTGTTATGACTTTATTTGCATATCCTGTGGAAGAAGATTTAGGTGAGATAGTTGTAGCAACTACCCGAATAGAGACGATGCTCGGTGATACTGCTATCGCAGTGCATCCTGATGACGAAAGATATAAGCATCTTCATGGAAAACATGCAATCCATCCATTTAATGGAAGAAGGATTCCTATAATTACCCCGGCCCATGATCCTAATGACTTTATGGTTGGGAAGCGCCATAATCTTGAGTTTATCAACATCTTTACTGATGATGGAAAAATAAACCAGTATGGGGGAGCATTTGAAGGGATGCCACGCTTCAGAGCCCGAGAGGCTGTGACTGATGCATTAAAGAAGAAGGGCCTCTTCAAAGAAACTAAGACCAATAAGATGTGTCTTAGAATTTGCTCTAGAAGTAAAGATGTTGTGGAGCCTATGATAAAGCCCCAGTGGTATATTAAATGCAGCGACATGGGAAATGAAGCTCTCAATGCTGTCGCTGATGATGAAAATAGGAAGCTGGAGATTATCCCAAGACAGTATACTGCTGAATGGAAGAGGCCAGATGTGTTGCCATTCAGTTGCCGATGGATTCTCTCAGTGCTAAATGAAGCCATATCCAAAACTGTTATGTCATTGGAATCATTTGAGTTCTCGGATGCAGCAACTGCTGTTTATGCTTGGTGGCAGTACCAGCTGTGTGATGTCTTCATTGAGAATGTCAAACCTTACTTTTCTGGTAATGATCCAAAGTTTGCAACAGAGAGGGGTTTTGCACGGGACAGTCTGTGGGTATGTCTTGACAATGGCCTGAGGTTACTTCATCCTTTCATGCCGTATGTGACAGAAGAACTGTGGCAACGTCTGCCATCGCCAGGGGATTACAAAAGGGCATCATCAATTAGGAGTGCTCTGATAGTGGAGGAGTGTAGGGTCATGGAGTTAGCGGACTCCTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004832 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006438 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

356

Amino Acids

40.81

Weight (kDa)

5.43

Isoelectric Point (pI)

44.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Anticodon_1	PF08264	233 - 338	2.9e-25	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14610 AT1G27160
fragaria_vesca	FvH4_5g33250 FvH4_7g01700 FvH4_7g28940 FvH4_7g28940 FvH4_7g28940
malus_domestica	MD01G1196000.v1.1 MD03G1000400.v1.1 MD06G1107900.v1.1 MD07G1262800.v1.1 MD15G1255900.v1.1 MD15G1433500.v1.1 MD17G1256800.v1.1
prunus_persica	Prupe.1G576900_v2.0.a1 Prupe.1G576900_v2.0.a1 Prupe.2G289400_v2.0.a1 Prupe.2G289400_v2.0.a1
pyrus_communis	pycom01g20660 pycom03g00060 pycom07g24190 pycom15g38270 pycom17g25900
rosa_chinensis	RchiOBHm_Chr1g0376611 RchiOBHm_Chr2g0151751 RchiOBHm_Chr2g0151761 RchiOBHm_Chr5g0049821 RchiOBHm_Chr5g0066661 RchiOBHm_Chr7g0182401 RchiOBHm_Chr7g0218391 RchiOBHm_Chr7g0218401 RchiOBHm_Chr7g0218411 RchiOBHm_Chr7g0232241 RchiOBHm_Chr7g0232251 RchiOBHm_Chr7g0232281 RchiOBHm_Chr7g0232291 RchiOBHm_Chr7g0232301
rosa_laevigata	RLG00000001360 RLG00000001362 RLG00000001368 RLG00000005125 RLG00000005126 RLG00000026593
rosa_multiflora	Rmu_co8187388.1_g000001 Rmu_co8244885.1_g000001 Rmu_co8371739.1_g000001 Rmu_co8421977.1_g000001 Rmu_co8457289.1_g000001 Rmu_sc0000239.1_g000041 Rmu_sc0000927.1_g000001 Rmu_sc0000945.1_g000003 Rmu_sc0002042.1_g000017 Rmu_sc0004136.1_g000003 Rmu_sc0005294.1_g000036 Rmu_sc0017871.1_g000001 Rmu_sc0029500.1_g000001 Rmu_sc0037577.1_g000001 Rmu_sc0037577.1_g000002 Rmu_ssc0000034.1_g000001 Rmu_ssc0000115.1_g000001
rosa_roxburghii	Rroxscaffold_2G00112130 Rroxscaffold_3G00228490 Rroxscaffold_3G00228520 Rroxscaffold_3G00228530 Rroxscaffold_3G00228620 Rroxscaffold_3G00228650 Rroxscaffold_4G00281940
rosa_rugosa	Rorug01G0396800 Rorug01G0396800 Rorug06G0073800 Rorug06G0073900 Rorug06G0447200 Rorug06G0447200 Rorug06G0447300 Rorug06G0447400 Rorug07G0270800 Rorug07G0271800 Rorug07G0271900 Rorug07G0272000 Rorug07G0272200 Rorug07G0272300
rosa_samantha	Rh1AG413400 Rh1BG373100 Rh1CG021500 Rh1CG151000 Rh1CG387000 Rh1DG404000 Rh2BG502300 Rh2DG513300 Rh3AG126000 Rh4AG156700 Rh7AG050700 Rh7AG050800 Rh7AG425000 Rh7AG425500 Rh7AG426200 Rh7BG050400 Rh7BG399800 Rh7BG399900 Rh7BG400000 Rh7CG052000 Rh7CG445400 Rh7CG445600 Rh7CG445900 Rh7CG446000 Rh7DG417300
rosa_wichuraiana	Rw0G007060 Rw1G036250 Rw1G036280 Rw7G027040 Rw7G035320 Rw7G035350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	284
AccI	GTMKAC	1 cut(s)	653
AciI	CCGC	3 cut(s)	25, 30, 1058
AclWI	GGATC	4 cut(s)	47, 60, 314, 851
AcsI	RAATTY	2 cut(s)	116, 513
AcuI	CTGAAG	1 cut(s)	415
AfaI	GTAC	1 cut(s)	803
AfiI	CCNNNNNNNGG	3 cut(s)	284, 403, 986
AgsI	TTSAA	3 cut(s)	65, 416, 481
AjnI	CCWGG	1 cut(s)	985
AluBI	AGCT	3 cut(s)	596, 631, 808
AluI	AGCT	3 cut(s)	596, 631, 808
Alw21I	GWGCWC	1 cut(s)	1024
Alw26I	GTCTC	1 cut(s)	188
AlwI	GGATC	4 cut(s)	47, 60, 314, 851
AlwNI	CAGNNNCTG	1 cut(s)	779
Ama87I	CYCGRG	1 cut(s)	438
AoxI	GGCC	4 cut(s)	312, 472, 671, 919
ApeKI	GCWGC	2 cut(s)	576, 773
ApoI	RAATTY	2 cut(s)	116, 513
AspLEI	GCGC	1 cut(s)	348
AspS9I	GGNCC	3 cut(s)	27, 313, 472
AsuC2I	CCSGG	1 cut(s)	311
AsuHPI	GGTGA	3 cut(s)	16, 176, 218
AvaI	CYCGRG	1 cut(s)	438
AvaII	GGWCC	1 cut(s)	27
AxyI	CCTNAGG	1 cut(s)	923
BamHI	GGATCC	1 cut(s)	52
BanII	GRGCYC	1 cut(s)	439
BbsI	GAAGAC	1 cut(s)	811
Bbv12I	GWGCWC	1 cut(s)	1024
BbvI	GCAGC	2 cut(s)	588, 785
BccI	CCATC	4 cut(s)	282, 377, 693, 988
BceAI	ACGGC	1 cut(s)	931
BciT130I	CCWGG	1 cut(s)	987
BcnI	CCSGG	1 cut(s)	311
BcoDI	GTCTC	1 cut(s)	188
BfaI	CTAG	2 cut(s)	86, 522
BfoI	RGCGCY	1 cut(s)	349
BglI	GCCNNNNNGGC	1 cut(s)	443
BisI	GCNGC	2 cut(s)	577, 774
BlsI	GCNGC	2 cut(s)	578, 775
Bme1390I	CCNGG	2 cut(s)	311, 987
Bme18I	GGWCC	1 cut(s)	27
BmeT110I	CYCGRG	1 cut(s)	438
BmgT120I	GGNCC	3 cut(s)	27, 313, 472
BmiI	GGNNCC	2 cut(s)	54, 543
BmrFI	CCNGG	2 cut(s)	311, 987
BmrI	ACTGGG	2 cut(s)	19, 553
BmsI	GCATC	7 cut(s)	189, 235, 259, 411, 445, 760, 1013
BmuI	ACTGGG	2 cut(s)	19, 553
BpiI	GAAGAC	1 cut(s)	811
BpmI	CTGGAG	1 cut(s)	653
BpuEI	CTTGAG	1 cut(s)	377
BpuMI	CCSGG	1 cut(s)	311
BsaJI	CCNNGG	2 cut(s)	309, 986
Bsc4I	CCNNNNNNNGG	3 cut(s)	284, 403, 986
Bse1I	ACTGG	3 cut(s)	14, 397, 559
Bse21I	CCTNAGG	1 cut(s)	923
BseBI	CCWGG	1 cut(s)	987
BseDI	CCNNGG	2 cut(s)	309, 986
BseGI	GGATG	5 cut(s)	226, 274, 426, 775, 934
BseLI	CCNNNNNNNGG	3 cut(s)	284, 403, 986
BseMII	CTCAG	2 cut(s)	723, 914
BseNI	ACTGG	3 cut(s)	14, 397, 559
BseRI	GAGGAG	1 cut(s)	1049
BseXI	GCAGC	2 cut(s)	588, 785
BshFI	GGCC	4 cut(s)	314, 474, 673, 921
BsiHKAI	GWGCWC	1 cut(s)	1024
BsiHKCI	CYCGRG	1 cut(s)	438
BsiSI	CCGG	1 cut(s)	311
BslFI	GGGAC	1 cut(s)	905
BslI	CCNNNNNNNGG	3 cut(s)	284, 403, 986
BsmAI	GTCTC	1 cut(s)	188
BsmBI	CGTCTC	1 cut(s)	188
BsmFI	GGGAC	1 cut(s)	905
BsnI	GGCC	4 cut(s)	314, 474, 673, 921
BsoBI	CYCGRG	1 cut(s)	438
Bsp1286I	GDGCHC	2 cut(s)	439, 1024
Bsp143I	GATC	3 cut(s)	52, 319, 856
BspACI	CCGC	3 cut(s)	25, 30, 1058
BspANI	GGCC	4 cut(s)	314, 474, 673, 921
BspCNI	CTCAG	2 cut(s)	722, 915
BspLI	GGNNCC	2 cut(s)	54, 543
BspPI	GGATC	4 cut(s)	47, 60, 314, 851
BsrI	ACTGG	3 cut(s)	14, 397, 559
BssECI	CCNNGG	2 cut(s)	309, 986
BssMI	GATC	3 cut(s)	52, 319, 856
BssNAI	GTATAC	1 cut(s)	654
Bst1107I	GTATAC	1 cut(s)	654
Bst2UI	CCWGG	1 cut(s)	987
Bst4CI	ACNGT	4 cut(s)	651, 739, 896, 966
Bst6I	CTCTTC	2 cut(s)	482, 662
BstAPI	GCANNNNNTGC	2 cut(s)	779, 976
BstDEI	CTNAG	4 cut(s)	489, 509, 709, 923
BstF5I	GGATG	5 cut(s)	226, 274, 426, 775, 934
BstH2I	RGCGCY	1 cut(s)	349
BstHHI	GCGC	1 cut(s)	348
BstKTI	GATC	3 cut(s)	55, 322, 859
BstMAI	GTCTC	1 cut(s)	188
BstMBI	GATC	3 cut(s)	52, 319, 856
BstMWI	GCNNNNNNNGC	6 cut(s)	443, 602, 779, 788, 805, 976
BstNI	CCWGG	1 cut(s)	987
BstNSI	RCATGY	1 cut(s)	269
BstSCI	CCNGG	2 cut(s)	309, 985
BstV1I	GCAGC	2 cut(s)	588, 785
BstV2I	GAAGAC	1 cut(s)	811
BstX2I	RGATCY	1 cut(s)	52
BstYI	RGATCY	1 cut(s)	52
BstZ17I	GTATAC	1 cut(s)	654
Bsu36I	CCTNAGG	1 cut(s)	923
BsuRI	GGCC	4 cut(s)	314, 474, 673, 921
BtgZI	GCGATG	1 cut(s)	966
BtsCI	GGATG	5 cut(s)	226, 274, 426, 775, 934
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	3 cut(s)	228, 566, 717
CaiI	CAGNNNCTG	1 cut(s)	779
CfoI	GCGC	1 cut(s)	348
Cfr13I	GGNCC	3 cut(s)	27, 313, 472
CpoI	CGGWCCG	1 cut(s)	27
Csp6I	GTAC	1 cut(s)	802
CspI	CGGWCCG	1 cut(s)	27
CviAII	CATG	6 cut(s)	257, 266, 317, 583, 943, 1048
CviQI	GTAC	1 cut(s)	802
DdeI	CTNAG	4 cut(s)	489, 509, 709, 923
DpnI	GATC	3 cut(s)	54, 321, 858
DpnII	GATC	3 cut(s)	52, 319, 856
Eam1104I	CTCTTC	2 cut(s)	482, 662
EarI	CTCTTC	2 cut(s)	482, 662
Eco24I	GRGCYC	1 cut(s)	439
Eco47I	GGWCC	1 cut(s)	27
Eco57I	CTGAAG	1 cut(s)	415
Eco81I	CCTNAGG	1 cut(s)	923
Eco88I	CYCGRG	1 cut(s)	438
EcoO109I	RGGNCCY	1 cut(s)	472
EcoRII	CCWGG	1 cut(s)	985
EcoT22I	ATGCAT	1 cut(s)	460
EcoT38I	GRGCYC	1 cut(s)	439
Esp3I	CGTCTC	1 cut(s)	188
FaeI	CATG	6 cut(s)	260, 269, 320, 586, 946, 1051
FalI	AAGNNNNNCTT	2 cut(s)	461, 493
FaqI	GGGAC	1 cut(s)	905
FatI	CATG	6 cut(s)	256, 265, 316, 582, 942, 1047
FblI	GTMKAC	1 cut(s)	653
Fnu4HI	GCNGC	2 cut(s)	577, 774
FokI	GGATG	5 cut(s)	213, 261, 433, 782, 921
FriOI	GRGCYC	1 cut(s)	439
Fsp4HI	GCNGC	2 cut(s)	577, 774
FspBI	CTAG	2 cut(s)	86, 522
GlaI	GCGC	1 cut(s)	347
GluI	GCNGC	2 cut(s)	577, 774
GsuI	CTGGAG	1 cut(s)	653
HaeII	RGCGCY	1 cut(s)	349
HaeIII	GGCC	4 cut(s)	314, 474, 673, 921
HapII	CCGG	1 cut(s)	311
HhaI	GCGC	1 cut(s)	348
Hin1II	CATG	6 cut(s)	260, 269, 320, 586, 946, 1051
Hin6I	GCGC	1 cut(s)	346
HinP1I	GCGC	1 cut(s)	346
HinfI	GANTC	4 cut(s)	295, 703, 752, 1061
HpaII	CCGG	1 cut(s)	311
HphI	GGTGA	3 cut(s)	16, 176, 218
Hpy166II	GTNNAC	2 cut(s)	7, 654
Hpy188I	TCNGA	3 cut(s)	434, 769, 1026
Hpy188III	TCNNGA	5 cut(s)	96, 230, 356, 522, 911
Hpy8I	GTNNAC	2 cut(s)	7, 654
HpyAV	CCTTC	3 cut(s)	285, 410, 463
HpyCH4III	ACNGT	4 cut(s)	651, 739, 896, 966
HpyCH4IV	ACGT	1 cut(s)	973
HpyCH4V	TGCA	8 cut(s)	140, 226, 269, 458, 576, 773, 869, 887
HpyF10VI	GCNNNNNNNGC	6 cut(s)	443, 602, 779, 788, 805, 976
HpyF3I	CTNAG	4 cut(s)	489, 509, 709, 923
HpySE526I	ACGT	1 cut(s)	973
Hsp92II	CATG	6 cut(s)	260, 269, 320, 586, 946, 1051
HspAI	GCGC	1 cut(s)	346
Kzo9I	GATC	3 cut(s)	52, 319, 856
LmnI	GCTCC	2 cut(s)	407, 541
Lsp1109I	GCAGC	2 cut(s)	588, 785
LweI	GCATC	7 cut(s)	189, 235, 259, 411, 445, 760, 1013
MaeI	CTAG	2 cut(s)	86, 522
MaeII	ACGT	1 cut(s)	973
MaeIII	GTNAC	3 cut(s)	448, 927, 952
MalI	GATC	3 cut(s)	54, 321, 858
MboI	GATC	3 cut(s)	52, 319, 856
MboII	GAAGA	9 cut(s)	164, 167, 245, 300, 469, 478, 679, 811, 971
MfeI	CAATTG	1 cut(s)	60
MflI	RGATCY	1 cut(s)	52
MhlI	GDGCHC	2 cut(s)	439, 1024
MluCI	AATT	5 cut(s)	60, 116, 303, 513, 1011
MlyI	GAGTC	1 cut(s)	1055
MnlI	CCTC	8 cut(s)	40, 66, 436, 485, 663, 870, 918, 1027
Mph1103I	ATGCAT	1 cut(s)	460
MseI	TTAA	4 cut(s)	282, 461, 570, 1069
MslI	CAYNNNNRTG	2 cut(s)	231, 255
MspA1I	CMGCKG	1 cut(s)	808
MspI	CCGG	1 cut(s)	311
MspR9I	CCNGG	2 cut(s)	311, 987
MunI	CAATTG	1 cut(s)	60
MvaI	CCWGG	1 cut(s)	987
MwoI	GCNNNNNNNGC	6 cut(s)	443, 602, 779, 788, 805, 976
NciI	CCSGG	1 cut(s)	311
NdeII	GATC	3 cut(s)	52, 319, 856
NlaIII	CATG	6 cut(s)	260, 269, 320, 586, 946, 1051
NlaIV	GGNNCC	2 cut(s)	54, 543
NmuCI	GTSAC	2 cut(s)	448, 952
NsiI	ATGCAT	1 cut(s)	460
NspI	RCATGY	1 cut(s)	269
PfeI	GAWTC	3 cut(s)	295, 703, 752
PflMI	CCANNNNNTGG	1 cut(s)	284
PkrI	GCNGC	2 cut(s)	578, 775
PleI	GAGTC	1 cut(s)	1055
PpsI	GAGTC	1 cut(s)	1055
Psp6I	CCWGG	1 cut(s)	985
PspGI	CCWGG	1 cut(s)	985
PspN4I	GGNNCC	2 cut(s)	54, 543
PspPI	GGNCC	3 cut(s)	27, 313, 472
PstNI	CAGNNNCTG	1 cut(s)	779
PsuI	RGATCY	1 cut(s)	52
PvuII	CAGCTG	1 cut(s)	808
RsaI	GTAC	1 cut(s)	803
RsaNI	GTAC	1 cut(s)	802
RseI	CAYNNNNRTG	2 cut(s)	231, 255
Rsr2I	CGGWCCG	1 cut(s)	27
RsrII	CGGWCCG	1 cut(s)	27
SaqAI	TTAA	4 cut(s)	282, 461, 570, 1069
SatI	GCNGC	2 cut(s)	577, 774
Sau3AI	GATC	3 cut(s)	52, 319, 856
Sau96I	GGNCC	3 cut(s)	27, 313, 472
SchI	GAGTC	1 cut(s)	1055
ScrFI	CCNGG	2 cut(s)	311, 987
SduI	GDGCHC	2 cut(s)	439, 1024
SetI	ASST	8 cut(s)	51, 166, 598, 633, 810, 841, 929, 976
SfaNI	GCATC	7 cut(s)	189, 235, 259, 411, 445, 760, 1013
SinI	GGWCC	1 cut(s)	27
SmiMI	CAYNNNNRTG	2 cut(s)	231, 255
SmlI	CTYRAG	1 cut(s)	356
SmoI	CTYRAG	1 cut(s)	356
Sse9I	AATT	5 cut(s)	60, 116, 303, 513, 1011
SsiI	CCGC	3 cut(s)	25, 30, 1058
SspMI	CTAG	2 cut(s)	86, 522
StyD4I	CCNGG	2 cut(s)	309, 985
TaaI	ACNGT	4 cut(s)	651, 739, 896, 966
TaiI	ACGT	1 cut(s)	976
TaqI	TCGA	1 cut(s)	114
TasI	AATT	5 cut(s)	60, 116, 303, 513, 1011
TfiI	GAWTC	3 cut(s)	295, 703, 752
Tru1I	TTAA	4 cut(s)	282, 461, 570, 1069
Tru9I	TTAA	4 cut(s)	282, 461, 570, 1069
TscAI	CASTG	3 cut(s)	228, 566, 717
TseFI	GTSAC	2 cut(s)	448, 952
TseI	GCWGC	2 cut(s)	576, 773
Tsp45I	GTSAC	2 cut(s)	448, 952
TspDTI	ATGAA	7 cut(s)	245, 606, 633, 735, 811, 923, 931
TspRI	CASTG	3 cut(s)	228, 566, 717
Van91I	CCANNNNNTGG	1 cut(s)	284
VpaK11BI	GGWCC	1 cut(s)	27
XapI	RAATTY	2 cut(s)	116, 513
XbaI	TCTAGA	1 cut(s)	521
XceI	RCATGY	1 cut(s)	269
XcmI	CCANNNNNNNNNTGG	1 cut(s)	281
XmiI	GTMKAC	1 cut(s)	653
XspI	CTAG	2 cut(s)	86, 522
Zsp2I	ATGCAT	1 cut(s)	460

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.