Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1018 |
| AccI | GTMKAC | 1 cut(s) | 331 |
| AccIII | TCCGGA | 1 cut(s) | 1176 |
| AciI | CCGC | 4 cut(s) | 304, 364, 434, 445 |
| AclWI | GGATC | 2 cut(s) | 662, 1168 |
| AcsI | RAATTY | 1 cut(s) | 1315 |
| AdeI | CACNNNGTG | 1 cut(s) | 711 |
| AfaI | GTAC | 3 cut(s) | 464, 614, 653 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 390, 797, 1018, 1267, 1309 |
| AgsI | TTSAA | 5 cut(s) | 216, 1190, 1231, 1328, 1370 |
| AjnI | CCWGG | 2 cut(s) | 796, 930 |
| AleI | CACNNNNGTG | 1 cut(s) | 122 |
| AluBI | AGCT | 6 cut(s) | 135, 233, 619, 858, 1135, 1294 |
| AluI | AGCT | 6 cut(s) | 135, 233, 619, 858, 1135, 1294 |
| Alw26I | GTCTC | 7 cut(s) | 632, 696, 894, 918, 943, 1053, 1326 |
| AlwI | GGATC | 2 cut(s) | 662, 1168 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 590 |
| Ama87I | CYCGRG | 1 cut(s) | 74 |
| Aor13HI | TCCGGA | 1 cut(s) | 1176 |
| AoxI | GGCC | 4 cut(s) | 57, 98, 381, 730 |
| ApeKI | GCWGC | 4 cut(s) | 107, 584, 1076, 1241 |
| ApoI | RAATTY | 1 cut(s) | 1315 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1242, 1274 |
| AspS9I | GGNCC | 2 cut(s) | 361, 1258 |
| AsuC2I | CCSGG | 1 cut(s) | 995 |
| AsuHPI | GGTGA | 1 cut(s) | 786 |
| AvaI | CYCGRG | 1 cut(s) | 74 |
| AvaII | GGWCC | 2 cut(s) | 361, 1258 |
| AxyI | CCTNAGG | 1 cut(s) | 734 |
| BauI | CACGAG | 1 cut(s) | 699 |
| BbsI | GAAGAC | 1 cut(s) | 622 |
| BbvI | GCAGC | 4 cut(s) | 94, 596, 1063, 1228 |
| BccI | CCATC | 3 cut(s) | 76, 407, 1020 |
| BceAI | ACGGC | 1 cut(s) | 775 |
| BciT130I | CCWGG | 2 cut(s) | 798, 932 |
| BclI | TGATCA | 2 cut(s) | 18, 846 |
| BcnI | CCSGG | 1 cut(s) | 995 |
| BcoDI | GTCTC | 7 cut(s) | 632, 696, 894, 918, 943, 1053, 1326 |
| BfaI | CTAG | 1 cut(s) | 1086 |
| BfmI | CTRYAG | 1 cut(s) | 265 |
| BisI | GCNGC | 4 cut(s) | 108, 585, 1077, 1242 |
| BlsI | GCNGC | 4 cut(s) | 109, 586, 1078, 1243 |
| Bme1390I | CCNGG | 3 cut(s) | 798, 932, 995 |
| Bme18I | GGWCC | 2 cut(s) | 361, 1258 |
| BmeT110I | CYCGRG | 1 cut(s) | 74 |
| BmgT120I | GGNCC | 2 cut(s) | 361, 1258 |
| BmiI | GGNNCC | 2 cut(s) | 1010, 1260 |
| BmrFI | CCNGG | 3 cut(s) | 798, 932, 995 |
| BmrI | ACTGGG | 1 cut(s) | 298 |
| BmsI | GCATC | 5 cut(s) | 167, 427, 571, 824, 1151 |
| BmuI | ACTGGG | 1 cut(s) | 298 |
| BpiI | GAAGAC | 1 cut(s) | 622 |
| BpmI | CTGGAG | 2 cut(s) | 72, 1137 |
| BpuEI | CTTGAG | 2 cut(s) | 1115, 1325 |
| BpuMI | CCSGG | 1 cut(s) | 995 |
| Bsa29I | ATCGAT | 1 cut(s) | 1062 |
| BsaI | GGTCTC | 1 cut(s) | 632 |
| BsaJI | CCNNGG | 3 cut(s) | 797, 930, 931 |
| BsaWI | WCCGGW | 1 cut(s) | 1176 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 940, 970 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 390, 797, 1018, 1267, 1309 |
| Bse1I | ACTGG | 2 cut(s) | 293, 1023 |
| Bse21I | CCTNAGG | 1 cut(s) | 734 |
| BseAI | TCCGGA | 1 cut(s) | 1176 |
| BseBI | CCWGG | 2 cut(s) | 798, 932 |
| BseCI | ATCGAT | 1 cut(s) | 1062 |
| BseDI | CCNNGG | 3 cut(s) | 797, 930, 931 |
| BseGI | GGATG | 4 cut(s) | 99, 208, 442, 745 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 390, 797, 1018, 1267, 1309 |
| BseMII | CTCAG | 6 cut(s) | 153, 414, 485, 591, 725, 1254 |
| BseNI | ACTGG | 2 cut(s) | 293, 1023 |
| BseRI | GAGGAG | 2 cut(s) | 179, 182 |
| BseXI | GCAGC | 4 cut(s) | 94, 596, 1063, 1228 |
| BshFI | GGCC | 4 cut(s) | 59, 100, 383, 732 |
| BshVI | ATCGAT | 1 cut(s) | 1062 |
| BsiHKCI | CYCGRG | 1 cut(s) | 74 |
| BsiSI | CCGG | 2 cut(s) | 994, 1177 |
| BslFI | GGGAC | 3 cut(s) | 39, 270, 1244 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 390, 797, 1018, 1267, 1309 |
| BsmAI | GTCTC | 7 cut(s) | 632, 696, 894, 918, 943, 1053, 1326 |
| BsmBI | CGTCTC | 2 cut(s) | 1053, 1326 |
| BsmFI | GGGAC | 3 cut(s) | 39, 270, 1244 |
| BsmI | GAATGC | 2 cut(s) | 194, 547 |
| BsnI | GGCC | 4 cut(s) | 59, 100, 383, 732 |
| Bso31I | GGTCTC | 1 cut(s) | 632 |
| BsoBI | CYCGRG | 1 cut(s) | 74 |
| Bsp13I | TCCGGA | 1 cut(s) | 1176 |
| Bsp143I | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| BspACI | CCGC | 4 cut(s) | 304, 364, 434, 445 |
| BspANI | GGCC | 4 cut(s) | 59, 100, 383, 732 |
| BspCNI | CTCAG | 6 cut(s) | 154, 415, 484, 590, 726, 1255 |
| BspDI | ATCGAT | 1 cut(s) | 1062 |
| BspEI | TCCGGA | 1 cut(s) | 1176 |
| BspLI | GGNNCC | 2 cut(s) | 1010, 1260 |
| BspPI | GGATC | 2 cut(s) | 662, 1168 |
| BspTNI | GGTCTC | 1 cut(s) | 632 |
| BsrI | ACTGG | 2 cut(s) | 293, 1023 |
| BssECI | CCNNGG | 3 cut(s) | 797, 930, 931 |
| BssMI | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| BssSI | CACGAG | 1 cut(s) | 699 |
| Bst2BI | CACGAG | 1 cut(s) | 699 |
| Bst2UI | CCWGG | 2 cut(s) | 798, 932 |
| Bst4CI | ACNGT | 7 cut(s) | 13, 518, 550, 643, 651, 777, 1057 |
| Bst6I | CTCTTC | 1 cut(s) | 1323 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 590, 787 |
| BstC8I | GCNNGC | 5 cut(s) | 71, 1035, 1239, 1288, 1292 |
| BstDEI | CTNAG | 6 cut(s) | 162, 423, 471, 577, 734, 1263 |
| BstEII | GGTNACC | 1 cut(s) | 512 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1307 |
| BstF5I | GGATG | 4 cut(s) | 99, 208, 442, 745 |
| BstKTI | GATC | 5 cut(s) | 21, 670, 849, 923, 1176 |
| BstMAI | GTCTC | 7 cut(s) | 632, 696, 894, 918, 943, 1053, 1326 |
| BstMBI | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 590, 599, 616, 787 |
| BstNI | CCWGG | 2 cut(s) | 798, 932 |
| BstNSI | RCATGY | 1 cut(s) | 1203 |
| BstPI | GGTNACC | 1 cut(s) | 512 |
| BstSCI | CCNGG | 3 cut(s) | 796, 930, 993 |
| BstSFI | CTRYAG | 1 cut(s) | 265 |
| BstV1I | GCAGC | 4 cut(s) | 94, 596, 1063, 1228 |
| BstV2I | GAAGAC | 1 cut(s) | 622 |
| BstX2I | RGATCY | 1 cut(s) | 1173 |
| BstYI | RGATCY | 1 cut(s) | 1173 |
| Bsu15I | ATCGAT | 1 cut(s) | 1062 |
| Bsu36I | CCTNAGG | 1 cut(s) | 734 |
| BsuRI | GGCC | 4 cut(s) | 59, 100, 383, 732 |
| BsuTUI | ATCGAT | 1 cut(s) | 1062 |
| BtgZI | GCGATG | 1 cut(s) | 1373 |
| BtsCI | GGATG | 4 cut(s) | 99, 208, 442, 745 |
| BtsI | GCAGTG | 3 cut(s) | 30, 117, 465 |
| BtsIMutI | CAGTG | 5 cut(s) | 9, 30, 117, 465, 527 |
| Cac8I | GCNNGC | 5 cut(s) | 71, 1035, 1239, 1288, 1292 |
| CaiI | CAGNNNCTG | 1 cut(s) | 590 |
| Cfr13I | GGNCC | 2 cut(s) | 361, 1258 |
| ClaI | ATCGAT | 1 cut(s) | 1062 |
| Csp6I | GTAC | 3 cut(s) | 463, 613, 652 |
| CviAII | CATG | 5 cut(s) | 335, 553, 754, 1139, 1200 |
| CviQI | GTAC | 3 cut(s) | 463, 613, 652 |
| DdeI | CTNAG | 6 cut(s) | 162, 423, 471, 577, 734, 1263 |
| DpnI | GATC | 5 cut(s) | 20, 669, 848, 922, 1175 |
| DpnII | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| DraIII | CACNNNGTG | 1 cut(s) | 711 |
| Eam1104I | CTCTTC | 1 cut(s) | 1323 |
| EarI | CTCTTC | 1 cut(s) | 1323 |
| Eco147I | AGGCCT | 1 cut(s) | 100 |
| Eco31I | GGTCTC | 1 cut(s) | 632 |
| Eco47I | GGWCC | 2 cut(s) | 361, 1258 |
| Eco81I | CCTNAGG | 1 cut(s) | 734 |
| Eco88I | CYCGRG | 1 cut(s) | 74 |
| Eco91I | GGTNACC | 1 cut(s) | 512 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1307 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1258 |
| EcoO65I | GGTNACC | 1 cut(s) | 512 |
| EcoRII | CCWGG | 2 cut(s) | 796, 930 |
| EcoT22I | ATGCAT | 1 cut(s) | 1144 |
| Esp3I | CGTCTC | 2 cut(s) | 1053, 1326 |
| FaeI | CATG | 5 cut(s) | 338, 556, 757, 1142, 1203 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 53, 85 |
| FaqI | GGGAC | 3 cut(s) | 39, 270, 1244 |
| FatI | CATG | 5 cut(s) | 334, 552, 753, 1138, 1199 |
| FauNDI | CATATG | 1 cut(s) | 760 |
| FbaI | TGATCA | 2 cut(s) | 18, 846 |
| FblI | GTMKAC | 1 cut(s) | 331 |
| Fnu4HI | GCNGC | 4 cut(s) | 108, 585, 1077, 1242 |
| FokI | GGATG | 4 cut(s) | 106, 215, 449, 732 |
| Fsp4HI | GCNGC | 4 cut(s) | 108, 585, 1077, 1242 |
| FspBI | CTAG | 1 cut(s) | 1086 |
| GluI | GCNGC | 4 cut(s) | 108, 585, 1077, 1242 |
| GsuI | CTGGAG | 2 cut(s) | 72, 1137 |
| HaeIII | GGCC | 4 cut(s) | 59, 100, 383, 732 |
| HapII | CCGG | 2 cut(s) | 994, 1177 |
| Hin1II | CATG | 5 cut(s) | 338, 556, 757, 1142, 1203 |
| HincII | GTYRAC | 1 cut(s) | 1340 |
| HindII | GTYRAC | 1 cut(s) | 1340 |
| HindIII | AAGCTT | 2 cut(s) | 231, 1133 |
| HinfI | GANTC | 5 cut(s) | 27, 258, 563, 1298, 1331 |
| HpaII | CCGG | 2 cut(s) | 994, 1177 |
| HphI | GGTGA | 1 cut(s) | 786 |
| Hpy166II | GTNNAC | 4 cut(s) | 286, 332, 465, 1340 |
| Hpy188I | TCNGA | 7 cut(s) | 163, 319, 474, 492, 580, 920, 1053 |
| Hpy188III | TCNNGA | 8 cut(s) | 51, 76, 359, 722, 941, 1154, 1177, 1325 |
| Hpy8I | GTNNAC | 4 cut(s) | 286, 332, 465, 1340 |
| HpyAV | CCTTC | 2 cut(s) | 1261, 1303 |
| HpyCH4III | ACNGT | 7 cut(s) | 13, 518, 550, 643, 651, 777, 1057 |
| HpyCH4IV | ACGT | 2 cut(s) | 784, 1207 |
| HpyCH4V | TGCA | 7 cut(s) | 174, 227, 311, 545, 584, 680, 1142 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 590, 599, 616, 787 |
| HpyF3I | CTNAG | 6 cut(s) | 162, 423, 471, 577, 734, 1263 |
| HpySE526I | ACGT | 2 cut(s) | 784, 1207 |
| Hsp92II | CATG | 5 cut(s) | 338, 556, 757, 1142, 1203 |
| Kpn2I | TCCGGA | 1 cut(s) | 1176 |
| Ksp22I | TGATCA | 2 cut(s) | 18, 846 |
| Kzo9I | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| LmnI | GCTCC | 1 cut(s) | 1084 |
| Lsp1109I | GCAGC | 4 cut(s) | 94, 596, 1063, 1228 |
| LweI | GCATC | 5 cut(s) | 167, 427, 571, 824, 1151 |
| MaeI | CTAG | 1 cut(s) | 1086 |
| MaeII | ACGT | 2 cut(s) | 784, 1207 |
| MaeIII | GTNAC | 8 cut(s) | 512, 527, 662, 738, 763, 792, 989, 1270 |
| MalI | GATC | 5 cut(s) | 20, 669, 848, 922, 1175 |
| MboI | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| MboII | GAAGA | 4 cut(s) | 622, 782, 1234, 1340 |
| MflI | RGATCY | 1 cut(s) | 1173 |
| MluCI | AATT | 6 cut(s) | 371, 395, 822, 1023, 1315, 1377 |
| MlyI | GAGTC | 3 cut(s) | 21, 252, 1340 |
| MmeI | TCCRAC | 1 cut(s) | 1106 |
| Mph1103I | ATGCAT | 1 cut(s) | 1144 |
| MroI | TCCGGA | 1 cut(s) | 1176 |
| MseI | TTAA | 2 cut(s) | 398, 1026 |
| MslI | CAYNNNNRTG | 1 cut(s) | 122 |
| MspA1I | CMGCKG | 1 cut(s) | 619 |
| MspI | CCGG | 2 cut(s) | 994, 1177 |
| MspR9I | CCNGG | 3 cut(s) | 798, 932, 995 |
| Mva1269I | GAATGC | 2 cut(s) | 194, 547 |
| MvaI | CCWGG | 2 cut(s) | 798, 932 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 590, 599, 616, 787 |
| NciI | CCSGG | 1 cut(s) | 995 |
| NdeI | CATATG | 1 cut(s) | 760 |
| NdeII | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| NlaIII | CATG | 5 cut(s) | 338, 556, 757, 1142, 1203 |
| NlaIV | GGNNCC | 2 cut(s) | 1010, 1260 |
| NmuCI | GTSAC | 3 cut(s) | 763, 792, 989 |
| NsiI | ATGCAT | 1 cut(s) | 1144 |
| NspI | RCATGY | 1 cut(s) | 1203 |
| OliI | CACNNNNGTG | 1 cut(s) | 122 |
| PaeR7I | CTCGAG | 1 cut(s) | 74 |
| PasI | CCCWGGG | 1 cut(s) | 931 |
| PceI | AGGCCT | 1 cut(s) | 100 |
| PctI | GAATGC | 2 cut(s) | 194, 547 |
| PfeI | GAWTC | 2 cut(s) | 563, 1298 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1018 |
| PkrI | GCNGC | 4 cut(s) | 109, 586, 1078, 1243 |
| PleI | GAGTC | 3 cut(s) | 21, 252, 1339 |
| PpsI | GAGTC | 3 cut(s) | 21, 252, 1339 |
| PpuMI | RGGWCCY | 1 cut(s) | 1258 |
| Psp5II | RGGWCCY | 1 cut(s) | 1258 |
| Psp6I | CCWGG | 2 cut(s) | 796, 930 |
| PspEI | GGTNACC | 1 cut(s) | 512 |
| PspGI | CCWGG | 2 cut(s) | 796, 930 |
| PspN4I | GGNNCC | 2 cut(s) | 1010, 1260 |
| PspPI | GGNCC | 2 cut(s) | 361, 1258 |
| PspPPI | RGGWCCY | 1 cut(s) | 1258 |
| PstNI | CAGNNNCTG | 1 cut(s) | 590 |
| PsuI | RGATCY | 1 cut(s) | 1173 |
| PvuII | CAGCTG | 1 cut(s) | 619 |
| RsaI | GTAC | 3 cut(s) | 464, 614, 653 |
| RsaNI | GTAC | 3 cut(s) | 463, 613, 652 |
| RseI | CAYNNNNRTG | 1 cut(s) | 122 |
| SaqAI | TTAA | 2 cut(s) | 398, 1026 |
| SatI | GCNGC | 4 cut(s) | 108, 585, 1077, 1242 |
| Sau3AI | GATC | 5 cut(s) | 18, 667, 846, 920, 1173 |
| Sau96I | GGNCC | 2 cut(s) | 361, 1258 |
| SchI | GAGTC | 3 cut(s) | 21, 252, 1340 |
| ScrFI | CCNGG | 3 cut(s) | 798, 932, 995 |
| SfaNI | GCATC | 5 cut(s) | 167, 427, 571, 824, 1151 |
| SfcI | CTRYAG | 1 cut(s) | 265 |
| Sfr274I | CTCGAG | 1 cut(s) | 74 |
| SinI | GGWCC | 2 cut(s) | 361, 1258 |
| SlaI | CTCGAG | 1 cut(s) | 74 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 122 |
| SmlI | CTYRAG | 3 cut(s) | 74, 1130, 1304 |
| SmoI | CTYRAG | 3 cut(s) | 74, 1130, 1304 |
| Sse9I | AATT | 6 cut(s) | 371, 395, 822, 1023, 1315, 1377 |
| SseBI | AGGCCT | 1 cut(s) | 100 |
| SsiI | CCGC | 4 cut(s) | 304, 364, 434, 445 |
| SspMI | CTAG | 1 cut(s) | 1086 |
| StuI | AGGCCT | 1 cut(s) | 100 |
| StyD4I | CCNGG | 3 cut(s) | 796, 930, 993 |
| TaaI | ACNGT | 7 cut(s) | 13, 518, 550, 643, 651, 777, 1057 |
| TaiI | ACGT | 2 cut(s) | 787, 1210 |
| TaqI | TCGA | 3 cut(s) | 75, 1062, 1220 |
| TaqII | GACCGA | 1 cut(s) | 491 |
| TasI | AATT | 6 cut(s) | 371, 395, 822, 1023, 1315, 1377 |
| TfiI | GAWTC | 2 cut(s) | 563, 1298 |
| Tru1I | TTAA | 2 cut(s) | 398, 1026 |
| Tru9I | TTAA | 2 cut(s) | 398, 1026 |
| TscAI | CASTG | 5 cut(s) | 16, 37, 124, 472, 527 |
| TseFI | GTSAC | 3 cut(s) | 763, 792, 989 |
| TseI | GCWGC | 4 cut(s) | 107, 584, 1076, 1241 |
| Tsp45I | GTSAC | 3 cut(s) | 763, 792, 989 |
| TspDTI | ATGAA | 7 cut(s) | 622, 734, 742, 882, 1125, 1127, 1376 |
| TspRI | CASTG | 5 cut(s) | 16, 37, 124, 472, 527 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1018 |
| VpaK11BI | GGWCC | 2 cut(s) | 361, 1258 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1307 |
| XapI | RAATTY | 1 cut(s) | 1315 |
| XceI | RCATGY | 1 cut(s) | 1203 |
| XhoI | CTCGAG | 1 cut(s) | 74 |
| XmiI | GTMKAC | 1 cut(s) | 331 |
| XspI | CTAG | 1 cut(s) | 1086 |
| Zsp2I | ATGCAT | 1 cut(s) | 1144 |