Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0000332

Physical Location & Seq

Genomic Coordinates Reverse (-)

52669347 .. 52712518

Gene Structure 43172 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G035350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3120 bp

ATGATCGGGGCTTTCTTTGACAGGCGCAGTATAGACCCATGGGAGAGAAAGAAGAGAAGGGAAGAAAAGGCATTAGAGAAAGAGCTCAAAAAGGACAAAGCAAAAGCTAAAGAAGCCGTCAGAAAGCTTCAGGAACAAGCAAACTCTACAGACAGTGGAAAGAAGGGGAAGAACAATGTGAAGCGTTCAAAGTCCAACGAGGATGATGGTGATGTTGATAACAATGCCAAGGACCCAGAGATTCCATACAGCGAGAAGAAACTGTTGTCTCTTCAAATGGACAAACACTACAATCCAAGTGCTGTGGAGAAGTCAAGGTGTGAGTGGTGGGAGATAAGGGGCTTGTTTATGGCAGATGCTAAAAGCTCCAAACCTCCCTTTGTGATGTTGTATCCGCCTCCGAATGTCACAGGTGTGCTTCATCTAGGCCATGCTCTCACTGCTGCTAATGGGGACACTATAATACGCTGGCGGCGGATGTCTGGCGACAACACCTTGTTGTTAGAACCAATCACAACTCAGGTTGTTGTGGAGAAGAAGCTCATGTCCGAAACCGGGAAAACCAGGCATGATCTTGGTAGAGATGAGTTTGTGAAACAAGTTTGGGAGTGGAAAAAAGAACACGGTGGCACCATTTTGCAGCAGCTACGCCGTCTCGGTGCTTCATTGGACTTTTCGAGGGAGAGCTTTACAATGGACGAGAAATGCTCAAAGGCTGTGGTAGAAGCCTTTGTAAGGCTTTATAACAAAGGACTTATCTACAGGGATAATCGTATAGTCAACTGGGATTGCTCCCTGAAAACAGCAATATCAGAAATTGAGGTGGACTCTCTGGATATTCAAGGAAGGACGTTACTGAAGGTTCCTGGTTATGAGAACAAGGTAAAGTTCGGCCTTTTGACTACATTCGCATACCCTTTGGAAGAAGAAGGAGAGGTACTTGTGGCGACTACAAGAATAGAAACCATGCTTGGTGATACTGGTATTGCTGTGCATCCGGATGACAGAAGATATAAGCATCTTCATGGAAAACATGCCATTCATCCGTTCAGTGGAAGAAGAATCCCTATAATATGTGATGCAAAGCTTGTTGATCCAGAATTTGGAACTGGTGCTGTGAAGATTACCCCGGCGCATGATCCTAATGACTTCGAAGTTGGCAAGCGCCATAATCTTGAGTTTATCAACATCTTCACTGATGATGGAGAAATAAACCAGCATGGTGGAGAAAAATTTGCAGGGATGCCACGTTATAAAGCGCGAGACTTGGTGACTGAAGCACTCAAGGAGAAGGGGCTGTATAGGAAAACTGAGGTTAATGAGATGAAAATCAAGATTTGTTCTAGAAGCAACGATGTTGTGGAGCCCATGATGAAGCCTCAGTGGTATGTCAAATGCTCTGGTATGGCGCATGAAGCTCTCATTAGCGTGGAGGAGGATGAAAGTAACATGAAGAAGCTAGAGATCATCCCCAAAGAGTATACTGATCACTGGAAGAGATGGCTTGAGAACATTCGTGACTGGTGCATCTCGAGGCAACTTTGGTGGGGTCACCGTATTCCGGCATGGTATGCTGTTTTTGAGGATGATAAGCGTGAGGATTTTGGACTGTTGGATGACAGATGGGTGGTTGCAAGAAACTTGGAAGAGGCTAGGGCAGTGGCTGCTGAAAAATTTGGGCAAGAAAAGAAGTATGAATTAAGTGAGTCTACTCAGGACCCTGATGTGCTTGACACTTGGTTTTCTTCTGCTCTCTTCCCATTGTCTGTATTCGGCTGGCCAGATGATACAGAAGACTTTAAGGCATTCTATCCGTCTTCACTTCTTGAAACTGGGCATGACATTCTCTTTTTCTGGGTTGCTCGCATGGAAATCTGCTGCCTTTTCCAAAGGTACGTATATCTGCACCCAATGATTCGTGATGCACAAGGACGAAAAATGTCAAAGTCGTTGGGGAATGTGATTGATCCAGTTGATGTAATCAATGGTATTACCCTTGAAGGTTTACATGAGAAGCTTTCAAAAGGAAACTTGGACAAAAAGGAAATAGAAGTAGCCAAAGAGGGGCAAAAGAAGTCATTCCCTAATGGTATCAAGGAATGTGGTGCAGATGCTCTTCGTTTTGCTCTCATTGACTACACTGCACAGTCTGAAAATATAAATCTGGACATCAAGAGGGTGGTGGGATATCGACACTGGTGCAACAAACTATGGAATGCAGTAAGGTTTGCTATTCTAACACTTGGGGACGGTTATGTTCCACCCTCAAATGCAATTAATCCAGAGGAGCTTCCATTTATTTGTCAGTGGATACTCTCAGTACTAAACAAAACAATTGATAAGACTGTGTCCTCATTGGAGTCCTATAAGTTATCTGATGCAGCTAAGGCTGTATATAGTTGGTGGCAGTACCAGTTATGCGATATTTTTATTGAAGGTATCAAGCCTTTCCTTTCTTATAATGATCCAACAGCAGAGTTTGCGAAACACACGCTATGGTTATGTCTTGATAATGGCCTGAGATTGCTTCATCCTTTTATGCCCTTCATCACAGAAGAGTTGTGGCAACGTCTTCATCTTCATGCAGCACCATCATCAATAATGGTAAGTGAATATCCATCCATGGTAGATTGCTGGAAGAACGAAAAAGTAGAGAATGAGATGGATAATCTTGTTATGTTTGTTGTGAATGCTCTGCGTTCACTAGCAAAAGGAAGTCGTCAACGGCGGCCAGGTTTTGTGCTATCTCAGGTGAAAGAAGAGAGACAATTGATATCCACTCAATCTGTCATTATTTCTCAATTAACCAATGTGTGGCCGTTGAAACATGCCTGCTACTTTATGAACTTTGAAATCATCAAGATATGGCATGCCCTCCTGGGAGTTGTGACTTCTCAACTTGAAGAGCAGCCAAGGACCCCAAAGAAGTTACGTTTTCCAGCATCGAGTGAATTACAAGCATATTCAATTTCTTCGATTGCCTCTCATGTTCCCTGTTGGTATAAAATATTAGCTGTTGTCAGAATCCTAAACACGAATAGAATTGGGGAAATATTGAGAATTTTTTTGTTTTTTAATAAAAAATCACAGGAGTACTTGCCCTCAAAGACGACACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004832 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006438 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

1039

Amino Acids

119.78

Weight (kDa)

7.56

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	107 - 723	7e-121	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1_2	PF13603	342 - 401	7.2e-08	Leucyl-tRNA synthetase, editing domain
Anticodon_1	PF08264	769 - 902	3.6e-29	Anticodon-binding domain of tRNA ligase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000332)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14610 AT1G27160
fragaria_vesca	FvH4_5g33250 FvH4_7g01700 FvH4_7g28940 FvH4_7g28940 FvH4_7g28940
malus_domestica	MD01G1196000.v1.1 MD03G1000400.v1.1 MD06G1107900.v1.1 MD07G1262800.v1.1 MD15G1255900.v1.1 MD15G1433500.v1.1 MD17G1256800.v1.1
prunus_persica	Prupe.1G576900_v2.0.a1 Prupe.1G576900_v2.0.a1 Prupe.2G289400_v2.0.a1 Prupe.2G289400_v2.0.a1
pyrus_communis	pycom01g20660 pycom03g00060 pycom07g24190 pycom15g38270 pycom17g25900
rosa_chinensis	RchiOBHm_Chr1g0376611 RchiOBHm_Chr2g0151751 RchiOBHm_Chr2g0151761 RchiOBHm_Chr5g0049821 RchiOBHm_Chr5g0066661 RchiOBHm_Chr7g0182401 RchiOBHm_Chr7g0218391 RchiOBHm_Chr7g0218401 RchiOBHm_Chr7g0218411 RchiOBHm_Chr7g0232241 RchiOBHm_Chr7g0232251 RchiOBHm_Chr7g0232281 RchiOBHm_Chr7g0232291 RchiOBHm_Chr7g0232301
rosa_laevigata	RLG00000001360 RLG00000001362 RLG00000001368 RLG00000005125 RLG00000005126 RLG00000026593
rosa_multiflora	Rmu_co8187388.1_g000001 Rmu_co8244885.1_g000001 Rmu_co8371739.1_g000001 Rmu_co8421977.1_g000001 Rmu_co8457289.1_g000001 Rmu_sc0000239.1_g000041 Rmu_sc0000927.1_g000001 Rmu_sc0000945.1_g000003 Rmu_sc0002042.1_g000017 Rmu_sc0004136.1_g000003 Rmu_sc0005294.1_g000036 Rmu_sc0017871.1_g000001 Rmu_sc0029500.1_g000001 Rmu_sc0037577.1_g000001 Rmu_sc0037577.1_g000002 Rmu_ssc0000034.1_g000001 Rmu_ssc0000115.1_g000001
rosa_roxburghii	Rroxscaffold_2G00112130 Rroxscaffold_3G00228490 Rroxscaffold_3G00228520 Rroxscaffold_3G00228530 Rroxscaffold_3G00228620 Rroxscaffold_3G00228650 Rroxscaffold_4G00281940
rosa_rugosa	Rorug01G0396800 Rorug01G0396800 Rorug06G0073800 Rorug06G0073900 Rorug06G0447200 Rorug06G0447200 Rorug06G0447300 Rorug06G0447400 Rorug07G0270800 Rorug07G0271800 Rorug07G0271900 Rorug07G0272000 Rorug07G0272200 Rorug07G0272300
rosa_samantha	Rh1AG413400 Rh1BG373100 Rh1CG021500 Rh1CG151000 Rh1CG387000 Rh1DG404000 Rh2BG502300 Rh2DG513300 Rh3AG126000 Rh4AG156700 Rh7AG050700 Rh7AG050800 Rh7AG425000 Rh7AG425500 Rh7AG426200 Rh7BG050400 Rh7BG399800 Rh7BG399900 Rh7BG400000 Rh7CG052000 Rh7CG445400 Rh7CG445600 Rh7CG445900 Rh7CG446000 Rh7DG417300
rosa_wichuraiana	Rw0G007060 Rw1G036250 Rw1G036280 Rw7G027040 Rw7G035320 Rw7G035350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	744, 1254, 2460
AccB1I	GGYRCC	1 cut(s)	629
AccB7I	CCANNNNNTGG	2 cut(s)	1103, 2814
AccI	GTMKAC	2 cut(s)	1481, 1709
AccII	CGCG	1 cut(s)	1261
AccIII	TCCGGA	1 cut(s)	997
AciI	CCGC	4 cut(s)	395, 472, 475, 2728
AclWI	GGATC	4 cut(s)	1088, 1133, 1961, 2459
AcoI	YGGCCR	3 cut(s)	1778, 2729, 2816
AcsI	RAATTY	4 cut(s)	1100, 1232, 1673, 3060
AcuI	CTGAAG	3 cut(s)	113, 878, 1296
AfaI	GTAC	5 cut(s)	939, 1895, 2322, 2411, 3095
AfiI	CCNNNNNNNGG	7 cut(s)	555, 735, 1052, 1103, 1561, 2064, 2814
AjnI	CCWGG	4 cut(s)	563, 865, 2731, 2877
AjuI	GAANNNNNNNTTGG	2 cut(s)	954, 986
AleI	CACNNNNGTG	1 cut(s)	413
Alw21I	GWGCWC	1 cut(s)	87
Alw26I	GTCTC	4 cut(s)	273, 659, 1257, 2758
AlwI	GGATC	4 cut(s)	1088, 1133, 1961, 2459
AlwNI	CAGNNNCTG	1 cut(s)	1664
Ama87I	CYCGRG	1 cut(s)	1531
Aor13HI	TCCGGA	1 cut(s)	997
AoxI	GGCC	6 cut(s)	427, 892, 1778, 2515, 2729, 2816
ApeKI	GCWGC	8 cut(s)	443, 640, 643, 1664, 1878, 2381, 2585, 2908
ApoI	RAATTY	4 cut(s)	1100, 1232, 1673, 3060
AseI	ATTAAT	1 cut(s)	2277
Asp700I	GAANNNNTTC	1 cut(s)	2078
AspLEI	GCGC	5 cut(s)	27, 1135, 1167, 1261, 1411
AspS9I	GGNCC	3 cut(s)	232, 1717, 2916
AsuC2I	CCSGG	2 cut(s)	556, 1130
AsuHPI	GGTGA	5 cut(s)	221, 986, 1282, 1544, 2764
AsuII	TTCGAA	1 cut(s)	1152
AvaI	CYCGRG	1 cut(s)	1531
AvaII	GGWCC	3 cut(s)	232, 1717, 2916
BalI	TGGCCA	1 cut(s)	1780
BanI	GGYRCC	1 cut(s)	629
BanII	GRGCYC	2 cut(s)	87, 1368
BarI	GAAGNNNNNNTAC	2 cut(s)	921, 953
BbsI	GAAGAC	3 cut(s)	1800, 1809, 2564
Bbv12I	GWGCWC	1 cut(s)	87
BbvI	GCAGC	8 cut(s)	430, 652, 655, 1651, 1865, 2393, 2597, 2920
BccI	CCATC	7 cut(s)	200, 1196, 1494, 1617, 2599, 2626, 2656
BceAI	ACGGC	4 cut(s)	101, 636, 2741, 2803
BcgI	CGANNNNNNTGC	2 cut(s)	2181, 2215
BciT130I	CCWGG	4 cut(s)	565, 867, 2733, 2879
BciVI	GTATCC	2 cut(s)	402, 2304
BclI	TGATCA	1 cut(s)	1486
BcnI	CCSGG	2 cut(s)	556, 1130
BcoDI	GTCTC	4 cut(s)	273, 659, 1257, 2758
BfaI	CTAG	5 cut(s)	425, 1344, 1460, 1653, 2705
BfmI	CTRYAG	2 cut(s)	147, 760
BfoI	RGCGCY	1 cut(s)	1168
BfuI	GTATCC	2 cut(s)	402, 2304
BmcAI	AGTACT	2 cut(s)	2322, 3095
Bme1390I	CCNGG	6 cut(s)	556, 565, 867, 1130, 2733, 2879
Bme18I	GGWCC	3 cut(s)	232, 1717, 2916
BmeT110I	CYCGRG	1 cut(s)	1531
BmgT120I	GGNCC	3 cut(s)	232, 1717, 2916
BmiI	GGNNCC	6 cut(s)	234, 631, 864, 1365, 1719, 2918
BmrFI	CCNGG	6 cut(s)	556, 565, 867, 1130, 2733, 2879
BmrI	ACTGGG	2 cut(s)	793, 1842
BmuI	ACTGGG	2 cut(s)	793, 1842
BpiI	GAAGAC	3 cut(s)	1800, 1809, 2564
BplI	GAGNNNNNCTC	4 cut(s)	692, 724, 812, 844
Bpu10I	CCTNAGC	1 cut(s)	2385
Bpu14I	TTCGAA	1 cut(s)	1152
BpuEI	CTTGAG	3 cut(s)	1196, 1268, 1526
BpuMI	CCSGG	2 cut(s)	556, 1130
BsaAI	YACGTR	1 cut(s)	1897
BsaBI	GATNNNNATC	1 cut(s)	1328
BsaJI	CCNNGG	6 cut(s)	38, 228, 1128, 2622, 2878, 2912
BsaWI	WCCGGW	1 cut(s)	997
Bsc4I	CCNNNNNNNGG	7 cut(s)	555, 735, 1052, 1103, 1561, 2064, 2814
Bse1I	ACTGG	9 cut(s)	788, 985, 1114, 1496, 1526, 1837, 1970, 2201, 2413
Bse8I	GATNNNNATC	1 cut(s)	1328
BseAI	TCCGGA	1 cut(s)	997
BseBI	CCWGG	4 cut(s)	565, 867, 2733, 2879
BseDI	CCNNGG	6 cut(s)	38, 228, 1128, 2622, 2878, 2912
BseJI	GATNNNNATC	1 cut(s)	1328
BseLI	CCNNNNNNNGG	7 cut(s)	555, 735, 1052, 1103, 1561, 2064, 2814
BseMII	CTCAG	7 cut(s)	533, 1302, 1394, 1727, 2331, 2510, 2762
BseNI	ACTGG	9 cut(s)	788, 985, 1114, 1496, 1526, 1837, 1970, 2201, 2413
BseRI	GAGGAG	2 cut(s)	1448, 2300
BseXI	GCAGC	8 cut(s)	430, 652, 655, 1651, 1865, 2393, 2597, 2920
BsgI	GTGCAG	3 cut(s)	1889, 2127, 2127
Bsh1236I	CGCG	1 cut(s)	1261
BshFI	GGCC	6 cut(s)	429, 894, 1780, 2517, 2731, 2818
BshNI	GGYRCC	1 cut(s)	629
BsiHKAI	GWGCWC	1 cut(s)	87
BsiHKCI	CYCGRG	1 cut(s)	1531
BsiSI	CCGG	4 cut(s)	555, 998, 1130, 1562
BslFI	GGGAC	2 cut(s)	467, 2261
BslI	CCNNNNNNNGG	7 cut(s)	555, 735, 1052, 1103, 1561, 2064, 2814
BsmAI	GTCTC	4 cut(s)	273, 659, 1257, 2758
BsmBI	CGTCTC	1 cut(s)	659
BsmFI	GGGAC	2 cut(s)	467, 2261
BsmI	GAATGC	3 cut(s)	1805, 2221, 2695
BsnI	GGCC	6 cut(s)	429, 894, 1780, 2517, 2731, 2818
BsoBI	CYCGRG	1 cut(s)	1531
Bsp119I	TTCGAA	1 cut(s)	1152
Bsp1286I	GDGCHC	2 cut(s)	87, 1368
Bsp13I	TCCGGA	1 cut(s)	997
Bsp143I	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
Bsp19I	CCATGG	2 cut(s)	38, 2622
BspACI	CCGC	4 cut(s)	395, 472, 475, 2728
BspANI	GGCC	6 cut(s)	429, 894, 1780, 2517, 2731, 2818
BspCNI	CTCAG	7 cut(s)	532, 1303, 1393, 1726, 2330, 2511, 2761
BspEI	TCCGGA	1 cut(s)	997
BspFNI	CGCG	1 cut(s)	1261
BspLI	GGNNCC	6 cut(s)	234, 631, 864, 1365, 1719, 2918
BspPI	GGATC	4 cut(s)	1088, 1133, 1961, 2459
BspQI	GCTCTTC	2 cut(s)	2121, 2898
BspT104I	TTCGAA	1 cut(s)	1152
BspT107I	GGYRCC	1 cut(s)	629
BsrI	ACTGG	9 cut(s)	788, 985, 1114, 1496, 1526, 1837, 1970, 2201, 2413
BssECI	CCNNGG	6 cut(s)	38, 228, 1128, 2622, 2878, 2912
BssMI	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
BssNAI	GTATAC	1 cut(s)	1482
BssT1I	CCWWGG	4 cut(s)	38, 228, 2622, 2912
Bst1107I	GTATAC	1 cut(s)	1482
Bst2UI	CCWGG	4 cut(s)	565, 867, 2733, 2879
Bst4CI	ACNGT	8 cut(s)	155, 264, 626, 1556, 1611, 2148, 2252, 2347
Bst6I	CTCTTC	9 cut(s)	47, 276, 1490, 1641, 1760, 2121, 2550, 2754, 2898
BstAPI	GCANNNNNTGC	3 cut(s)	1571, 1664, 2480
BstBAI	YACGTR	1 cut(s)	1897
BstBI	TTCGAA	1 cut(s)	1152
BstC8I	GCNNGC	6 cut(s)	470, 1163, 1778, 1864, 2833, 2871
BstDEI	CTNAG	8 cut(s)	519, 1311, 1380, 1713, 2317, 2385, 2519, 2748
BstDSI	CCRYGG	2 cut(s)	38, 2622
BstEII	GGTNACC	1 cut(s)	1550
BstENI	CCTNNNNNAGG	1 cut(s)	733
BstFNI	CGCG	1 cut(s)	1261
BstH2I	RGCGCY	1 cut(s)	1168
BstHHI	GCGC	5 cut(s)	27, 1135, 1167, 1261, 1411
BstKTI	GATC	8 cut(s)	6, 574, 1096, 1141, 1467, 1489, 1969, 2467
BstMAI	GTCTC	4 cut(s)	273, 659, 1257, 2758
BstMBI	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
BstMWI	GCNNNNNNNGC	7 cut(s)	113, 440, 1415, 1571, 1664, 2387, 2480
BstNI	CCWGG	4 cut(s)	565, 867, 2733, 2879
BstNSI	RCATGY	3 cut(s)	1037, 2831, 2873
BstPI	GGTNACC	1 cut(s)	1550
BstSCI	CCNGG	6 cut(s)	554, 563, 865, 1128, 2731, 2877
BstSFI	CTRYAG	2 cut(s)	147, 760
BstSNI	TACGTA	1 cut(s)	1897
BstUI	CGCG	1 cut(s)	1261
BstV1I	GCAGC	8 cut(s)	430, 652, 655, 1651, 1865, 2393, 2597, 2920
BstV2I	GAAGAC	3 cut(s)	1800, 1809, 2564
BstXI	CCANNNNNNTGG	1 cut(s)	1223
BstZ17I	GTATAC	1 cut(s)	1482
BsuI	GTATCC	2 cut(s)	402, 2304
BsuRI	GGCC	6 cut(s)	429, 894, 1780, 2517, 2731, 2818
BtgI	CCRYGG	2 cut(s)	38, 2622
BtsI	GCAGTG	3 cut(s)	438, 1665, 2139
Cac8I	GCNNGC	6 cut(s)	470, 1163, 1778, 1864, 2833, 2871
CaiI	CAGNNNCTG	1 cut(s)	1664
CfoI	GCGC	5 cut(s)	27, 1135, 1167, 1261, 1411
Cfr13I	GGNCC	3 cut(s)	232, 1717, 2916
Csp6I	GTAC	5 cut(s)	938, 1894, 2321, 2410, 3094
CspCI	CAANNNNNGTGG	8 cut(s)	285, 320, 699, 734, 1526, 1561, 2083, 2118
CviQI	GTAC	5 cut(s)	938, 1894, 2321, 2410, 3094
DdeI	CTNAG	8 cut(s)	519, 1311, 1380, 1713, 2317, 2385, 2519, 2748
DpnI	GATC	8 cut(s)	5, 573, 1095, 1140, 1466, 1488, 1968, 2466
DpnII	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
EaeI	YGGCCR	3 cut(s)	1778, 2729, 2816
Eam1104I	CTCTTC	9 cut(s)	47, 276, 1490, 1641, 1760, 2121, 2550, 2754, 2898
EarI	CTCTTC	9 cut(s)	47, 276, 1490, 1641, 1760, 2121, 2550, 2754, 2898
EciI	GGCGGA	2 cut(s)	384, 490
Ecl136II	GAGCTC	1 cut(s)	85
Eco105I	TACGTA	1 cut(s)	1897
Eco130I	CCWWGG	4 cut(s)	38, 228, 2622, 2912
Eco24I	GRGCYC	2 cut(s)	87, 1368
Eco32I	GATATC	2 cut(s)	2189, 2775
Eco47I	GGWCC	3 cut(s)	232, 1717, 2916
Eco53kI	GAGCTC	1 cut(s)	85
Eco57I	CTGAAG	3 cut(s)	113, 878, 1296
Eco88I	CYCGRG	1 cut(s)	1531
Eco91I	GGTNACC	1 cut(s)	1550
EcoICRI	GAGCTC	1 cut(s)	85
EcoNI	CCTNNNNNAGG	1 cut(s)	733
EcoO109I	RGGNCCY	3 cut(s)	232, 1717, 2916
EcoO65I	GGTNACC	1 cut(s)	1550
EcoRII	CCWGG	4 cut(s)	563, 865, 2731, 2877
EcoRV	GATATC	2 cut(s)	2189, 2775
EcoT14I	CCWWGG	4 cut(s)	38, 228, 2622, 2912
EcoT38I	GRGCYC	2 cut(s)	87, 1368
ErhI	CCWWGG	4 cut(s)	38, 228, 2622, 2912
Esp3I	CGTCTC	1 cut(s)	659
FaqI	GGGAC	2 cut(s)	467, 2261
FbaI	TGATCA	1 cut(s)	1486
FblI	GTMKAC	2 cut(s)	1481, 1709
FriOI	GRGCYC	2 cut(s)	87, 1368
FspBI	CTAG	5 cut(s)	425, 1344, 1460, 1653, 2705
GlaI	GCGC	5 cut(s)	26, 1134, 1166, 1260, 1410
HaeII	RGCGCY	1 cut(s)	1168
HaeIII	GGCC	6 cut(s)	429, 894, 1780, 2517, 2731, 2818
HapII	CCGG	4 cut(s)	555, 998, 1130, 1562
HhaI	GCGC	5 cut(s)	27, 1135, 1167, 1261, 1411
Hin6I	GCGC	5 cut(s)	25, 1133, 1165, 1259, 1409
HinP1I	GCGC	5 cut(s)	25, 1133, 1165, 1259, 1409
HincII	GTYRAC	2 cut(s)	781, 2723
HindII	GTYRAC	2 cut(s)	781, 2723
HindIII	AAGCTT	3 cut(s)	125, 1085, 2015
HinfI	GANTC	7 cut(s)	241, 827, 1062, 1706, 1915, 2360, 3024
HpaII	CCGG	4 cut(s)	555, 998, 1130, 1562
HphI	GGTGA	5 cut(s)	221, 986, 1282, 1544, 2764
Hpy166II	GTNNAC	7 cut(s)	781, 826, 1482, 1710, 2006, 2702, 2723
Hpy188I	TCNGA	7 cut(s)	122, 402, 550, 814, 2152, 2377, 3023
Hpy8I	GTNNAC	7 cut(s)	781, 826, 1482, 1710, 2006, 2702, 2723
HpyAV	CCTTC	9 cut(s)	51, 157, 840, 853, 923, 1285, 1994, 2429, 2554
HpyCH4III	ACNGT	8 cut(s)	155, 264, 626, 1556, 1611, 2148, 2252, 2347
HpyCH4IV	ACGT	5 cut(s)	851, 1249, 1896, 2569, 2932
HpyF10VI	GCNNNNNNNGC	7 cut(s)	113, 440, 1415, 1571, 1664, 2387, 2480
HpyF3I	CTNAG	8 cut(s)	519, 1311, 1380, 1713, 2317, 2385, 2519, 2748
HpySE526I	ACGT	5 cut(s)	851, 1249, 1896, 2569, 2932
HspAI	GCGC	5 cut(s)	25, 1133, 1165, 1259, 1409
Kpn2I	TCCGGA	1 cut(s)	997
Ksp22I	TGATCA	1 cut(s)	1486
Kzo9I	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
LguI	GCTCTTC	2 cut(s)	2121, 2898
LmnI	GCTCC	4 cut(s)	371, 797, 1363, 2287
Lsp1109I	GCAGC	8 cut(s)	430, 652, 655, 1651, 1865, 2393, 2597, 2920
MaeI	CTAG	5 cut(s)	425, 1344, 1460, 1653, 2705
MaeII	ACGT	5 cut(s)	851, 1249, 1896, 2569, 2932
MaeIII	GTNAC	8 cut(s)	406, 852, 1270, 1445, 1517, 1550, 2887, 2928
MalI	GATC	8 cut(s)	5, 573, 1095, 1140, 1466, 1488, 1968, 2466
MboI	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
MfeI	CAATTG	2 cut(s)	2334, 2768
MhlI	GDGCHC	2 cut(s)	87, 1368
MlsI	TGGCCA	1 cut(s)	1780
MluNI	TGGCCA	1 cut(s)	1780
MlyI	GAGTC	3 cut(s)	821, 1715, 2369
MmeI	TCCRAC	3 cut(s)	219, 1593, 2492
Mox20I	TGGCCA	1 cut(s)	1780
MroI	TCCGGA	1 cut(s)	997
MroXI	GAANNNNTTC	1 cut(s)	2078
MscI	TGGCCA	1 cut(s)	1780
MseI	TTAA	6 cut(s)	1317, 1700, 1800, 2277, 2804, 3075
MslI	CAYNNNNRTG	5 cut(s)	413, 999, 1023, 1427, 2580
Msp20I	TGGCCA	1 cut(s)	1780
MspI	CCGG	4 cut(s)	555, 998, 1130, 1562
MspR9I	CCNGG	6 cut(s)	556, 565, 867, 1130, 2733, 2879
MunI	CAATTG	2 cut(s)	2334, 2768
Mva1269I	GAATGC	3 cut(s)	1805, 2221, 2695
MvaI	CCWGG	4 cut(s)	565, 867, 2733, 2879
MvnI	CGCG	1 cut(s)	1261
MwoI	GCNNNNNNNGC	7 cut(s)	113, 440, 1415, 1571, 1664, 2387, 2480
NciI	CCSGG	2 cut(s)	556, 1130
NcoI	CCATGG	2 cut(s)	38, 2622
NdeII	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
NlaIV	GGNNCC	6 cut(s)	234, 631, 864, 1365, 1719, 2918
NmuCI	GTSAC	5 cut(s)	406, 1270, 1517, 1550, 2887
NspI	RCATGY	3 cut(s)	1037, 2831, 2873
NspV	TTCGAA	1 cut(s)	1152
OliI	CACNNNNGTG	1 cut(s)	413
PaeI	GCATGC	1 cut(s)	2873
PaeR7I	CTCGAG	1 cut(s)	1531
PciSI	GCTCTTC	2 cut(s)	2121, 2898
PctI	GAATGC	3 cut(s)	1805, 2221, 2695
PdmI	GAANNNNTTC	1 cut(s)	2078
PfeI	GAWTC	4 cut(s)	241, 1062, 1915, 3024
PflFI	GACNNNGTC	1 cut(s)	2347
PflMI	CCANNNNNTGG	2 cut(s)	1103, 2814
PleI	GAGTC	3 cut(s)	821, 1714, 2368
PpsI	GAGTC	3 cut(s)	821, 1714, 2368
Ppu21I	YACGTR	1 cut(s)	1897
PpuMI	RGGWCCY	3 cut(s)	232, 1717, 2916
PshBI	ATTAAT	1 cut(s)	2277
PsiI	TTATAA	3 cut(s)	744, 1254, 2460
Psp124BI	GAGCTC	1 cut(s)	87
Psp5II	RGGWCCY	3 cut(s)	232, 1717, 2916
Psp6I	CCWGG	4 cut(s)	563, 865, 2731, 2877
PspEI	GGTNACC	1 cut(s)	1550
PspGI	CCWGG	4 cut(s)	563, 865, 2731, 2877
PspN4I	GGNNCC	6 cut(s)	234, 631, 864, 1365, 1719, 2918
PspPI	GGNCC	3 cut(s)	232, 1717, 2916
PspPPI	RGGWCCY	3 cut(s)	232, 1717, 2916
PstNI	CAGNNNCTG	1 cut(s)	1664
PsyI	GACNNNGTC	1 cut(s)	2347
RsaI	GTAC	5 cut(s)	939, 1895, 2322, 2411, 3095
RsaNI	GTAC	5 cut(s)	938, 1894, 2321, 2410, 3094
RseI	CAYNNNNRTG	5 cut(s)	413, 999, 1023, 1427, 2580
SacI	GAGCTC	1 cut(s)	87
SapI	GCTCTTC	2 cut(s)	2121, 2898
SaqAI	TTAA	6 cut(s)	1317, 1700, 1800, 2277, 2804, 3075
Sau3AI	GATC	8 cut(s)	3, 571, 1093, 1138, 1464, 1486, 1966, 2464
Sau96I	GGNCC	3 cut(s)	232, 1717, 2916
ScaI	AGTACT	2 cut(s)	2322, 3095
SchI	GAGTC	3 cut(s)	821, 1715, 2369
ScrFI	CCNGG	6 cut(s)	556, 565, 867, 1130, 2733, 2879
SduI	GDGCHC	2 cut(s)	87, 1368
SfcI	CTRYAG	2 cut(s)	147, 760
Sfr274I	CTCGAG	1 cut(s)	1531
SfuI	TTCGAA	1 cut(s)	1152
SinI	GGWCC	3 cut(s)	232, 1717, 2916
SlaI	CTCGAG	1 cut(s)	1531
SmiMI	CAYNNNNRTG	5 cut(s)	413, 999, 1023, 1427, 2580
SmlI	CTYRAG	4 cut(s)	1175, 1283, 1505, 1531
SmoI	CTYRAG	4 cut(s)	1175, 1283, 1505, 1531
SnaBI	TACGTA	1 cut(s)	1897
SphI	GCATGC	1 cut(s)	2873
SsiI	CCGC	4 cut(s)	395, 472, 475, 2728
SspI	AATATT	2 cut(s)	3009, 3054
SspMI	CTAG	5 cut(s)	425, 1344, 1460, 1653, 2705
SstI	GAGCTC	1 cut(s)	87
StyD4I	CCNGG	6 cut(s)	554, 563, 865, 1128, 2731, 2877
StyI	CCWWGG	4 cut(s)	38, 228, 2622, 2912
TaaI	ACNGT	8 cut(s)	155, 264, 626, 1556, 1611, 2148, 2252, 2347
TaiI	ACGT	5 cut(s)	854, 1252, 1899, 2572, 2935
TaqI	TCGA	6 cut(s)	677, 1152, 1532, 2191, 2945, 2975
TatI	WGTACW	2 cut(s)	2320, 3093
TauI	GCSGC	2 cut(s)	475, 2731
TfiI	GAWTC	4 cut(s)	241, 1062, 1915, 3024
Tru1I	TTAA	6 cut(s)	1317, 1700, 1800, 2277, 2804, 3075
Tru9I	TTAA	6 cut(s)	1317, 1700, 1800, 2277, 2804, 3075
TseFI	GTSAC	5 cut(s)	406, 1270, 1517, 1550, 2887
TseI	GCWGC	8 cut(s)	443, 640, 643, 1664, 1878, 2381, 2585, 2908
Tsp45I	GTSAC	5 cut(s)	406, 1270, 1517, 1550, 2887
TspGWI	ACGGA	2 cut(s)	1035, 1803
Tth111I	GACNNNGTC	1 cut(s)	2347
Van91I	CCANNNNNTGG	2 cut(s)	1103, 2814
VpaK11BI	GGWCC	3 cut(s)	232, 1717, 2916
VspI	ATTAAT	1 cut(s)	2277
XagI	CCTNNNNNAGG	1 cut(s)	733
XapI	RAATTY	4 cut(s)	1100, 1232, 1673, 3060
XbaI	TCTAGA	1 cut(s)	1343
XceI	RCATGY	3 cut(s)	1037, 2831, 2873
XhoI	CTCGAG	1 cut(s)	1531
XmiI	GTMKAC	2 cut(s)	1481, 1709
XmnI	GAANNNNTTC	1 cut(s)	2078
XspI	CTAG	5 cut(s)	425, 1344, 1460, 1653, 2705
ZrmI	AGTACT	2 cut(s)	2322, 3095

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.